Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,534 C→T 14.3% intergenic (+346/‑585) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5340C→T14.3% 208.2 ‑0.3 0.0615intergenic (+346/‑585)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (44/40):  ref base (244/242):  total (313/302)
Fisher's exact test strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 700 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGT  >  NC_014034/1395434‑1395634
                                                                                                       |                                                                                                       
gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                                                                                         >  1:5274391/1‑101 (MQ=255)
gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                                                                                         >  1:220432/1‑101 (MQ=255)
 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                                                                                        <  1:5406707/101‑1 (MQ=255)
 gcGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                                                                                        <  1:2671250/100‑1 (MQ=255)
  cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                                                                                       <  2:5466039/101‑1 (MQ=255)
    cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                                                                                    <  1:2804564/101‑5 (MQ=2)
    cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                                                                                                     <  1:5983300/101‑1 (MQ=255)
    cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                                                                                                     >  2:2797609/1‑101 (MQ=255)
     tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                                                                                    >  2:2033522/1‑101 (MQ=255)
     tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                                                                                    >  1:1021907/1‑101 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                                                                                <  2:7764576/101‑1 (MQ=255)
          ccGCGGGGGCGTGGATCGAAACACCCCGGACCTGTCGGCGG.CGA.ACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtcgatgatcaa                                                                                                <  1:6389223/101‑10 (MQ=0)
            gcgGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGGGCCGTCg                                                                                             >  2:161489/1‑101 (MQ=255)
              gggggCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                                                                                           >  1:2490001/1‑101 (MQ=255)
               ggggCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                                                                                          >  2:4617521/1‑101 (MQ=255)
                gggCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCg                                                                                         >  1:7569269/1‑101 (MQ=255)
                 ggCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.t                                                                                       <  2:5319858/101‑1 (MQ=255)
                  gCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tc                                                                                      >  1:4414197/1‑101 (MQ=255)
                   cGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                     <  1:8211035/101‑1 (MQ=255)
                   cGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                     >  2:3887663/1‑101 (MQ=255)
                   cGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                     >  1:4746214/1‑101 (MQ=255)
                   cGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                     <  2:3466314/101‑1 (MQ=255)
                    gTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                    >  1:7112904/1‑101 (MQ=255)
                    gTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                    >  1:548174/1‑101 (MQ=255)
                    gTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                    >  1:7962610/1‑101 (MQ=255)
                     tGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                   >  1:8673302/1‑101 (MQ=255)
                      ggATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcacgc                                                                                  >  2:8668522/1‑97 (MQ=255)
                      ggATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc                                                                                  >  2:7435126/1‑101 (MQ=255)
                        aTCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCCGCCg                                                                                >  2:4840182/1‑101 (MQ=255)
                          cGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.c                                                                             <  2:5161117/101‑1 (MQ=255)
                            aaaCATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                           <  1:139914/101‑1 (MQ=255)
                             aaCATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                         >  1:5613439/1‑101 (MQ=255)
                                      g..cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCt                                                                <  1:4859523/101‑1 (MQ=255)
                                      g..cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCt                                                                <  2:8040966/101‑1 (MQ=255)
                                          t.ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcc                                                              >  2:3634490/1‑101 (MQ=255)
                                          t.ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcc                                                              >  1:8460216/1‑101 (MQ=255)
                                            ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTccc                                                             >  1:9116857/1‑101 (MQ=255)
                                             tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.TCTCGTCGCTcccc                                                            >  1:4181644/1‑101 (MQ=255)
                                              ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTccccc                                                           <  1:7871644/101‑1 (MQ=255)
                                                 ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgc                                                        <  2:4972721/101‑1 (MQ=255)
                                                  gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgcg                                                       >  1:3874324/1‑101 (MQ=255)
                                                    cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggg                                                     <  2:876586/101‑1 (MQ=255)
                                                    cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggg                                                     <  1:7726037/101‑1 (MQ=255)
                                                      aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                   >  1:4504889/1‑101 (MQ=255)
                                                         ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                >  2:7599604/1‑101 (MQ=255)
                                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                               >  1:5107637/1‑101 (MQ=255)
                                                          gCTGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                               <  2:2984236/101‑1 (MQ=255)
                                                           aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                              >  2:2491168/11‑101 (MQ=2)
                                                           aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                              >  2:6806090/11‑101 (MQ=2)
                                                            tGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  2:9010832/101‑1 (MQ=255)
                                                            ccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:650085/92‑1 (MQ=2)
                                                            aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  2:3266448/10‑101 (MQ=0)
                                                             ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  2:594796/93‑1 (MQ=2)
                                                             ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:3724141/101‑1 (MQ=255)
                                                              gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:6252423/101‑1 (MQ=255)
                                                               gcggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  1:4326767/95‑1 (MQ=2)
                                                               gcggcggtCGCGCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  1:929655/95‑1 (MQ=2)
                                                               ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  1:162210/95‑1 (MQ=2)
                                                                cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         >  1:3976264/6‑101 (MQ=2)
                                                                aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         >  2:2902391/1‑101 (MQ=255)
                                                                 tgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  2:658029/5‑101 (MQ=2)
                                                                 ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCGCCGCGGGGGCGTGGATCGaa                                        >  1:7233711/5‑101 (MQ=2)
                                                                 aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  1:2353096/1‑101 (MQ=255)
                                                                 aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                        >  1:80515/1‑101 (MQ=255)
                                                                  gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  2:9117267/4‑101 (MQ=2)
                                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  1:504094/1‑101 (MQ=255)
                                                                   tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                      >  2:6099803/3‑101 (MQ=2)
                                                                   tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                      <  2:2238697/99‑1 (MQ=2)
                                                                   gtgtCCCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                      <  2:40575/101‑1 (MQ=255)
                                                                   gcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaca                                       >  2:8376901/3‑99 (MQ=2)
                                                                   acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                      <  2:5737871/99‑1 (MQ=2)
                                                                    tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATAGaaaac                                     >  2:1380596/1‑99 (MQ=255)
                                                                    ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                     <  1:6710342/100‑2 (MQ=2)
                                                                    cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                     <  2:1163501/100‑2 (MQ=2)
                                                                    cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCCCCCCCCGCGGGGGCGGGGATCGGAacc                                     >  2:7868358/2‑101 (MQ=0)
                                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                    <  2:7112600/101‑1 (MQ=255)
                                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                    >  1:3522516/1‑99 (MQ=2)
                                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGCGGagcccaacta                                    >  2:4247191/1‑92 (MQ=0)
                                                                      tCGCTCCCCCCGCGGGTGCGTGGATCGAAGC.GCCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                   <  2:1882764/101‑4 (MQ=0)
                                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                   <  1:5863125/101‑4 (MQ=2)
                                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                   >  2:854082/1‑98 (MQ=2)
                                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                  >  1:7442806/1‑97 (MQ=2)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                 <  1:2022478/101‑1 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                 <  2:6242314/101‑1 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                 >  1:3230067/1‑97 (MQ=2)
                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                >  1:6804024/1‑95 (MQ=2)
                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                <  1:1320837/101‑7 (MQ=2)
                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                >  1:861247/1‑95 (MQ=2)
                                                                          tCCCCGCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                               >  2:4520254/1‑101 (MQ=255)
                                                                          tCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcgg                               >  2:1787650/1‑95 (MQ=2)
                                                                           ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                              <  1:8908229/101‑9 (MQ=2)
                                                                           ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                              >  1:6147512/1‑94 (MQ=2)
                                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                              >  1:7659743/1‑93 (MQ=2)
                                                                            cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                             <  2:147876/101‑10 (MQ=2)
                                                                            cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                             <  2:6492797/101‑10 (MQ=2)
                                                                                     gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                    <  2:376693/101‑1 (MQ=255)
                                                                                        cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                 <  1:2899397/101‑1 (MQ=255)
                                                                                                  aaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa       >  1:1814906/1‑101 (MQ=255)
                                                                                                    cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg     >  1:3067444/1‑101 (MQ=255)
                                                                                                     aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCAAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc    >  2:7283846/1‑101 (MQ=255)
                                                                                                       |                                                                                                       
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGT  >  NC_014034/1395434‑1395634

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG