Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,537 G→T 1.9% intergenic (+349/‑582) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5370G→T 1.9% 16.1 0.0 ‑0.3630intergenic (+349/‑582)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (311/306):  total (318/312)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.82e-01

Only 100 of 660 total aligned reads displayed.

GCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGC  >  NC_014034/1395437‑1395637
                                                                                                    |                                                                                                      
gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                                                                        <  1:5409483/101‑1 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                                                                                                       >  2:2797609/1‑101 (MQ=255)
  tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                                                                                      >  1:1021907/1‑101 (MQ=255)
      cccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                                                                                  <  2:7864697/101‑1 (MQ=255)
      cccGCGGGGGCGTGGATCGAAACATCGCCGCTGTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                                                                                  <  1:4551165/101‑1 (MQ=255)
        cgcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCTTc                                                                                                <  2:8750039/101‑1 (MQ=255)
         gcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCg                                                                                               <  1:8005695/101‑1 (MQ=255)
               gCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tc                                                                                        <  1:8314668/101‑1 (MQ=255)
                ggTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                       <  2:4156515/100‑1 (MQ=255)
                cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                       <  1:5122420/101‑1 (MQ=255)
                  tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATTTCGGGGCCGTCGCTCG.TCCAg                                                                                     >  1:9129070/1‑101 (MQ=255)
                   ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc                                                                                    >  2:7435126/1‑101 (MQ=255)
                   ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc                                                                                    >  1:966265/1‑101 (MQ=255)
                     aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCCGCCg                                                                                  >  2:4840182/1‑101 (MQ=255)
                        gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                              >  1:9046347/1‑101 (MQ=255)
                         aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                             <  1:139914/101‑1 (MQ=255)
                         aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGGGGCGCTCCCCCCGCGGGGGGGTGGATGGAGACATCTCGGTGCCGTGCCTCG.CCCCGGCGG.CCt                                                                             >  1:2938768/1‑101 (MQ=255)
                              tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcg                                                                        <  2:705073/101‑1 (MQ=255)
                               cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcgt                                                                       >  1:6912365/1‑101 (MQ=255)
                               cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcgt                                                                       >  1:358467/1‑101 (MQ=255)
                               cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtcgt                                                                       <  1:5878758/101‑1 (MQ=255)
                                    cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTc                                                                  >  1:7935480/1‑101 (MQ=255)
                                    cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTc                                                                  <  2:1551433/101‑1 (MQ=255)
                                       tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGCGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTcccc                                                               >  1:627306/1‑101 (MQ=255)
                                         gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTcccccc                                                             <  2:5785393/101‑1 (MQ=255)
                                          cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcg                                                            <  2:6104329/101‑1 (MQ=255)
                                          cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcg                                                            <  2:7201468/101‑1 (MQ=255)
                                              cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCggg                                                        >  1:7692466/1‑101 (MQ=255)
                                                aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCggggg                                                      <  2:3730513/101‑1 (MQ=255)
                                                   ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGt                                                   >  2:7599604/1‑101 (MQ=255)
                                                    gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTg                                                  >  1:4016161/1‑101 (MQ=255)
                                                     ggcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                 <  1:2198193/91‑1 (MQ=0)
                                                     ggcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                 <  1:2630252/91‑1 (MQ=0)
                                                     gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTgg                                                 <  1:5062933/91‑1 (MQ=0)
                                                     cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                 >  1:2454346/1‑101 (MQ=255)
                                                     aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                 >  2:6806090/11‑101 (MQ=2)
                                                      tGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  2:9010832/101‑1 (MQ=255)
                                                      gcggctgcagtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                >  1:53226/10‑101 (MQ=0)
                                                      ccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  1:4193187/92‑1 (MQ=2)
                                                      aggccggtggtCGCTCCCCCTGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  1:5640027/92‑1 (MQ=0)
                                                      aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                >  2:3266448/10‑101 (MQ=0)
                                                       ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  2:594796/93‑1 (MQ=2)
                                                        gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCTGGGGCGTGGATc                                              <  1:6637602/94‑1 (MQ=2)
                                                        ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  2:387315/94‑1 (MQ=2)
                                                        ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                              >  1:6020223/8‑101 (MQ=2)
                                                         ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:162210/95‑1 (MQ=2)
                                                          cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                            >  2:8307371/6‑101 (MQ=2)
                                                          aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                            >  2:3522281/1‑101 (MQ=255)
                                                           tgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           >  2:658029/5‑101 (MQ=2)
                                                           ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           >  2:5405687/5‑101 (MQ=2)
                                                           aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           >  2:2117/1‑101 (MQ=255)
                                                           aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           >  1:2353096/1‑101 (MQ=255)
                                                            cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          >  2:1831356/4‑101 (MQ=2)
                                                            cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          <  1:7543215/98‑1 (MQ=2)
                                                            cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaca                                          >  2:4592983/1‑99 (MQ=255)
                                                            cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          <  1:8549270/101‑1 (MQ=255)
                                                            cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          >  1:6494940/1‑101 (MQ=255)
                                                             tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                         <  1:3786135/99‑1 (MQ=2)
                                                             tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                         >  1:3900503/3‑101 (MQ=2)
                                                              tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                        <  2:3037089/101‑1 (MQ=255)
                                                               gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAaccc                                       >  2:7748055/1‑100 (MQ=255)
                                                               gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                       >  1:721176/1‑100 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                      <  1:615905/101‑1 (MQ=255)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                      <  1:8136173/101‑3 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                      >  2:854082/1‑98 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                     >  1:5507219/1‑98 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                     >  1:2850166/1‑97 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                     <  1:4756590/101‑5 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                    <  2:6242314/101‑1 (MQ=255)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                    <  2:5282064/101‑1 (MQ=255)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                    >  1:7609220/1‑96 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                    >  1:8641261/1‑96 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGAGGATCGAAactgcgg                                    >  1:6786822/1‑96 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                    >  2:5132853/1‑97 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                   >  1:4869269/1‑101 (MQ=255)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                   <  1:1320837/101‑7 (MQ=2)
                                                                    tCCCCGCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                  >  2:4520254/1‑101 (MQ=255)
                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                  >  1:3141886/1‑94 (MQ=2)
                                                                    cccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                  <  2:4972082/100‑8 (MQ=2)
                                                                    cccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                  <  1:4434953/100‑8 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGAGGGGATCGAAaccccgcgga                                 >  2:2907277/1‑94 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                 >  2:1644556/1‑93 (MQ=2)
                                                                      cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                <  2:6492797/101‑10 (MQ=2)
                                                                       ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                               <  2:8649667/101‑1 (MQ=255)
                                                                               gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTAAc                       <  1:731139/101‑1 (MQ=255)
                                                                                ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                      >  1:437379/1‑101 (MQ=255)
                                                                                ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                      <  2:1939510/101‑1 (MQ=255)
                                                                                  cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                    >  2:4012143/1‑101 (MQ=255)
                                                                                   gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                   <  1:7805918/101‑1 (MQ=255)
                                                                                   gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                   <  2:141549/101‑1 (MQ=255)
                                                                                     ggACCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACGTTGCGGATTGTCACTTTcgc                 >  1:8835709/1‑101 (MQ=255)
                                                                                        tCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg              >  2:7974419/1‑101 (MQ=255)
                                                                                         cGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg             >  2:2565646/1‑101 (MQ=255)
                                                                                           aaaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg           >  1:3482576/1‑101 (MQ=255)
                                                                                           aaaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg           <  1:184963/101‑1 (MQ=255)
                                                                                              cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg        <  2:2284934/101‑1 (MQ=255)
                                                                                               aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc       <  2:1363474/101‑1 (MQ=255)
                                                                                                  tcGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc    <  2:4340612/101‑1 (MQ=255)
                                                                                                  tcGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc    <  2:9116435/101‑1 (MQ=255)
                                                                                                    ggTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGc  >  1:2031153/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
GCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGC  >  NC_014034/1395437‑1395637

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG