Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,547 C→A 7.0% intergenic (+359/‑572) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5470C→A 7.0% 91.0 ‑0.4 0.0625intergenic (+359/‑572)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (24/20):  ref base (289/291):  total (313/312)
Fisher's exact test strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 665 total aligned reads displayed.

CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCG  >  NC_014034/1395447‑1395647
                                                                                                     |                                                                                                        
cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGc                                                                                                          <  2:6124818/101‑1 (MQ=255)
cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGc                                                                                                          >  1:2618791/1‑101 (MQ=255)
 gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCt                                                                                                         >  1:2490001/1‑101 (MQ=255)
    ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.t                                                                                                     <  2:5319858/101‑1 (MQ=255)
    ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.t                                                                                                     <  1:6865199/101‑1 (MQ=255)
      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.Tcc                                                                                                   <  2:3466314/101‑1 (MQ=255)
      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.Tcc                                                                                                   >  1:4746214/1‑101 (MQ=255)
       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCa                                                                                                  <  2:7411876/101‑1 (MQ=255)
       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCa                                                                                                  <  1:7901781/101‑1 (MQ=255)
        tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAg                                                                                                 <  2:5174377/101‑1 (MQ=255)
         ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGc                                                                                                <  1:1329052/101‑1 (MQ=255)
         ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATC.TCGGTGCCGTCCCTCG.TCCCGc                                                                                                >  1:873700/1‑101 (MQ=255)
          gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGcc                                                                                               >  2:8620030/1‑101 (MQ=255)
              gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                          <  2:910809/101‑1 (MQ=255)
                aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                       >  1:168157/1‑101 (MQ=255)
                  cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.Gcc                                                                                     >  2:2022417/1‑100 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtc                                                                                    <  2:5797300/101‑1 (MQ=255)
                           tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TGGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcc                                                                            >  1:6572113/1‑101 (MQ=255)
                              ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTccccc                                                                         >  1:5322001/1‑101 (MQ=255)
                               gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcccccc                                                                        <  1:2797691/101‑1 (MQ=255)
                               gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcccccc                                                                        <  1:831375/101‑1 (MQ=255)
                                 ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgc                                                                      >  1:6151859/1‑101 (MQ=255)
                                  gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgcg                                                                     >  1:7196538/1‑101 (MQ=255)
                                    cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggg                                                                   <  1:7726037/101‑1 (MQ=255)
                                     gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCgggg                                                                  >  1:141549/1‑101 (MQ=255)
                                        aGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCg                                                               >  1:450028/1‑101 (MQ=255)
                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                             >  1:5107637/1‑101 (MQ=255)
                                           ggcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                            <  1:2198193/91‑1 (MQ=0)
                                           cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                            <  1:3839996/101‑1 (MQ=255)
                                           aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                                            <  1:2912554/91‑1 (MQ=2)
                                           aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                                            >  2:6806090/11‑101 (MQ=2)
                                            aggccggtggtCGCTCCCCCTGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                           <  1:5640027/92‑1 (MQ=0)
                                             ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                          <  2:3690671/93‑1 (MQ=2)
                                             ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                          >  2:7727392/1‑101 (MQ=255)
                                              ggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGCGGCGTGGATc                                                         >  1:7503487/8‑101 (MQ=0)
                                              ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:3841601/94‑1 (MQ=2)
                                              ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:6490177/94‑1 (MQ=2)
                                              atcccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.GATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                          <  2:4849076/94‑1 (MQ=0)
                                              ataccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.GATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                          >  2:5040457/8‑101 (MQ=0)
                                               ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        >  1:6783587/7‑101 (MQ=2)
                                               ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        >  1:7666141/7‑101 (MQ=2)
                                                cgcacgtggCTCCCCCCGGGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                       <  1:5869474/93‑1 (MQ=2)
                                                cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                       >  1:4033000/6‑101 (MQ=2)
                                                aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                       <  1:905733/101‑1 (MQ=255)
                                                 tgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      >  2:658029/5‑101 (MQ=2)
                                                 tgcggtCGCTCCCCCCGCGGGGGCGGGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      >  1:930313/5‑101 (MQ=2)
                                                 ggtggccGCGCCCCCCGCGGGCGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  2:7576541/95‑1 (MQ=2)
                                                 aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      >  1:2353096/1‑101 (MQ=255)
                                                  cgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.GATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  2:4073892/98‑1 (MQ=2)
                                                  cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     <  1:8131660/98‑1 (MQ=2)
                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     <  1:8549270/101‑1 (MQ=255)
                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     >  1:504094/1‑101 (MQ=255)
                                                   tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                    >  1:4249799/3‑101 (MQ=2)
                                                   acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                    <  2:3134892/99‑1 (MQ=2)
                                                    cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAact                                                   >  1:112315/2‑100 (MQ=2)
                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCG.GCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                   <  2:1869087/101‑2 (MQ=2)
                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                                  <  2:7112600/101‑1 (MQ=255)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                 >  1:7298447/1‑101 (MQ=255)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                                 <  1:6040862/101‑4 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcg                                                >  1:5409380/1‑97 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                                >  2:1601022/1‑98 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                                <  2:8977377/101‑4 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                >  1:4399587/1‑97 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                               <  2:6242314/101‑1 (MQ=255)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                               >  1:8641261/1‑96 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                               <  1:5537300/101‑6 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                              <  2:556544/101‑7 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                             >  2:8243296/1‑94 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcgg                                             >  2:1787650/1‑95 (MQ=2)
                                                           ccccccGGGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAtccccgcggc                                            <  2:5952817/101‑8 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                            >  2:2210904/1‑101 (MQ=255)
                                                           ccccccGCGGGGGCGTGGATCGAAACAAA.AAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                            <  1:3218659/101‑8 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                            >  2:143247/1‑93 (MQ=2)
                                                            cccccGCGGGGGCGTGGATCGAAACA.C.CCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                           <  2:6492797/101‑10 (MQ=2)
                                                             ccccGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                          <  1:3522388/101‑1 (MQ=255)
                                                             ccccGCGGGGGCGTGGATCGAAACAAT.GCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgggcgcg                                          >  2:8462096/1‑91 (MQ=2)
                                                                  cgGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGt                                    <  1:3966219/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCACCTTGCGGC.TTGGCAc                                 >  2:4543237/1‑101 (MQ=255)
                                                                     gggCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCAc                                 <  1:3680966/101‑1 (MQ=255)
                                                                      ggCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACt                                <  1:7599931/101‑1 (MQ=255)
                                                                       gCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACtt                               <  2:2870238/101‑1 (MQ=255)
                                                                       gCGTGGATCGAAACATC.TCGGTGCCGTCACTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACtt                               <  1:7963954/101‑1 (MQ=255)
                                                                        ggTGGATCGAAACATC.CCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACttt                              <  2:786285/100‑1 (MQ=255)
                                                                          tGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTGACTTTcc                            >  2:2964309/1‑100 (MQ=255)
                                                                          tGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTcg                            >  2:7261399/1‑101 (MQ=255)
                                                                            gATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGGGGA..TGTCACCTTcgccg                         >  2:8732183/1‑99 (MQ=38)
                                                                              tCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCggg                        >  2:7974419/1‑101 (MQ=255)
                                                                                gAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGc                      >  1:6294451/1‑101 (MQ=255)
                                                                                  aaCATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGa                    >  2:3974344/1‑101 (MQ=255)
                                                                                     aTC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGc                 <  2:1363474/101‑1 (MQ=255)
                                                                                     aTC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGc                 <  2:2711552/101‑1 (MQ=255)
                                                                                       c.tcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGt               >  1:4861963/1‑101 (MQ=255)
                                                                                          cGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTTGTC.CTTTCCGGGGGCGCAgggcgg             >  2:4274490/1‑96 (MQ=25)
                                                                                           ggTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGc            >  1:2031153/1‑101 (MQ=255)
                                                                                              gCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCCGA.TTGTCACTTTCGCGGGGCGAGGGGTccggcc         >  1:8950354/1‑96 (MQ=37)
                                                                                               ccGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTccc        <  1:1897454/101‑1 (MQ=255)
                                                                                                  tCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc     <  2:4742774/101‑1 (MQ=255)
                                                                                                  gcGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc     <  2:4414072/100‑1 (MQ=255)
                                                                                                  gatcTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc     <  1:3121553/98‑1 (MQ=255)
                                                                                                   cGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcg    >  2:245200/1‑101 (MQ=255)
                                                                                                     |                                                                                                        
CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATC.TCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCG  >  NC_014034/1395447‑1395647

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG