Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,552 C→G 33.4% intergenic (+364/‑567) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5520C→G33.4% 0.0 ‑0.1 ‑0.2625intergenic (+364/‑567)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (103/101):  ref base (207/200):  total (317/308)
Fisher's exact test strand distribution p-value = 9.32e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.83e-01

Only 100 of 663 total aligned reads displayed.

GCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395452‑1395652
                                                                                                     |                                                                                                       
 ggTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                                        <  2:4156515/100‑1 (MQ=255)
 cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                                        <  1:8211035/101‑1 (MQ=255)
  gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                       >  1:7112904/1‑101 (MQ=255)
  gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                       <  2:7411876/101‑1 (MQ=255)
  gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                       >  1:4373998/1‑101 (MQ=255)
   tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                                      >  1:8673302/1‑101 (MQ=255)
      aTCGAAACATCGTCGCTTTGGCGGTTGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCg                                                                                                   >  1:6527748/1‑101 (MQ=255)
        cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.c                                                                                                <  2:5161117/101‑1 (MQ=255)
        cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.c                                                                                                >  1:4000803/1‑101 (MQ=255)
         gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                               <  2:910809/101‑1 (MQ=255)
           aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                            >  1:168157/1‑101 (MQ=255)
           aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                            >  1:5613439/1‑101 (MQ=255)
              aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtc                                                                                         <  2:5797300/101‑1 (MQ=255)
               tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcg                                                                                        <  1:58239/101‑1 (MQ=255)
               tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcg                                                                                        <  2:705073/101‑1 (MQ=255)
                cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcgt                                                                                       >  1:358467/1‑101 (MQ=255)
                     cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTc                                                                                  >  1:7706014/1‑101 (MQ=255)
                     cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTc                                                                                  <  2:1551433/101‑1 (MQ=255)
                     cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTc                                                                                  >  1:7935480/1‑101 (MQ=255)
                      tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcc                                                                                 >  1:8460216/1‑101 (MQ=255)
                         ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTccccc                                                                              <  1:7871644/101‑1 (MQ=255)
                           cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcg                                                                            <  2:7201468/101‑1 (MQ=255)
                            ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgc                                                                           <  2:4972721/101‑1 (MQ=255)
                              tCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCgg                                                                         <  2:3329915/101‑1 (MQ=255)
                               cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgcgtg                                                                        >  1:6794157/1‑99 (MQ=255)
                               cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggg                                                                        >  1:2870336/1‑101 (MQ=255)
                                gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCgggg                                                                       >  1:141549/1‑101 (MQ=255)
                                      gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:5382477/91‑1 (MQ=0)
                                      gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:5924246/91‑1 (MQ=0)
                                      cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:5076726/101‑1 (MQ=255)
                                      cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:2284555/101‑1 (MQ=255)
                                      cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                                 >  2:4421483/1‑101 (MQ=255)
                                      agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:4856064/91‑1 (MQ=0)
                                        ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               >  2:7727392/1‑101 (MQ=255)
                                         gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  1:6252423/101‑1 (MQ=255)
                                         ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              >  2:2203188/8‑101 (MQ=2)
                                         ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  2:387315/94‑1 (MQ=2)
                                          taccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  1:4692891/95‑1 (MQ=2)
                                          tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:3495948/101‑1 (MQ=255)
                                           cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  1:3039163/6‑101 (MQ=2)
                                            gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  2:5325716/5‑101 (MQ=2)
                                            aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  2:1868206/101‑1 (MQ=255)
                                             cgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:9054990/98‑1 (MQ=2)
                                              tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                         <  1:3786135/99‑1 (MQ=2)
                                               ctacGCTCCCCCCGCGGGGGCGTGGATCCAAACATCGATGATCAAACCCACA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                         <  2:3071953/98‑1 (MQ=2)
                                               cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                        <  2:1163501/100‑2 (MQ=2)
                                               cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCCCCCCCCGCGGGGGCGGGGATCGGAacc                                                        >  2:7868358/2‑101 (MQ=0)
                                                gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                                       <  1:5491698/101‑3 (MQ=2)
                                                gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                       >  2:2678889/1‑99 (MQ=2)
                                                gtCCCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                                       <  1:4221552/101‑3 (MQ=2)
                                                 tCGCGCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGTGGCGTGGATCGAAactgc                                                      <  2:1699528/101‑4 (MQ=2)
                                                  ccgaccccccGCCGGGGCGTGGATCAAAACATCTCGTTTCCGTCCCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                     <  2:2942246/97‑1 (MQ=37)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcg                                                     <  1:3906554/101‑5 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                                     <  2:8977377/101‑4 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                     >  1:7613918/1‑97 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                                    <  1:2022478/101‑1 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                                    <  1:6713629/101‑1 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                                    >  2:5963056/1‑96 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                                    <  2:8596952/101‑6 (MQ=2)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                   <  2:7978084/101‑1 (MQ=255)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                                   <  1:1320837/101‑7 (MQ=2)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                   <  2:556544/101‑7 (MQ=2)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                   >  1:861247/1‑95 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTGCCCCCGCGGGGGCGCGGATCGAAactgcgtgc                                                  >  1:6103923/1‑94 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                  >  2:870401/1‑94 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                  >  2:8243296/1‑94 (MQ=2)
                                                     cccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                  <  1:4446809/100‑1 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                                 <  1:8908229/101‑9 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                 <  1:8021347/101‑8 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                 >  2:4891126/1‑93 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                 >  1:7567963/1‑93 (MQ=2)
                                                       cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                                <  2:6492797/101‑10 (MQ=2)
                                                          ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACACCTTGCGGA.t                                            <  1:9163856/101‑1 (MQ=255)
                                                            gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTg                                          <  2:2399238/101‑1 (MQ=255)
                                                                gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCACCTTGCGGC.TTGGCAc                                      >  2:4543237/1‑101 (MQ=255)
                                                                gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCAc                                      <  2:2862725/101‑1 (MQ=255)
                                                                gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCAc                                      <  2:2000483/101‑1 (MQ=255)
                                                                  gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACtt                                    >  1:5175997/1‑101 (MQ=255)
                                                                   cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACttt                                   >  1:722816/1‑101 (MQ=255)
                                                                   cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACttt                                   >  2:4012143/1‑101 (MQ=255)
                                                                    gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTc                                  <  2:141549/101‑1 (MQ=255)
                                                                            aaaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCg                          <  1:184963/101‑1 (MQ=255)
                                                                                  ctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGt                    >  2:3737941/1‑101 (MQ=255)
                                                                                    cGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTTGTC.CTTTCCGGGGGCGCAgggcgg                  >  2:4274490/1‑96 (MQ=25)
                                                                                      gTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCt                >  2:7559985/1‑101 (MQ=255)
                                                                                        gCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTcc              >  2:6163794/1‑101 (MQ=255)
                                                                                          cGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGACGCTcccc            >  1:6388189/1‑101 (MQ=255)
                                                                                            tCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc          <  2:4742774/101‑1 (MQ=255)
                                                                                            tCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc          >  2:6592739/1‑101 (MQ=255)
                                                                                             cGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcg         <  1:3161444/101‑1 (MQ=255)
                                                                                              gCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc        <  1:2515787/101‑1 (MQ=255)
                                                                                               cTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGCCGCTCCCCCcgcg       >  1:2724853/1‑101 (MQ=255)
                                                                                                tCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgg      >  1:104875/1‑101 (MQ=255)
                                                                                                 cG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCggg     >  1:7391149/1‑101 (MQ=255)
                                                                                                 cG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCggg     >  2:3561710/1‑101 (MQ=255)
                                                                                                  g.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg    <  1:4971061/101‑1 (MQ=255)
                                                                                                  g.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg    <  2:8459836/101‑1 (MQ=255)
                                                                                                  g.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg    >  2:6890821/1‑101 (MQ=255)
                                                                                                  g.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg    <  1:2182179/101‑1 (MQ=255)
                                                                                                     ccAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGc  <  2:627296/101‑1 (MQ=255)
                                                                                                     |                                                                                                       
GCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGA.TTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395452‑1395652

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG