Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,673 G→C 9.0% intergenic (+485/‑446) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6730G→C 9.0% 76.6 ‑2.6 ‑1.5391intergenic (+485/‑446)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/22):  ref base (192/163):  total (205/186)
Fisher's exact test strand distribution p-value = 7.50e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.07e-02

Only 100 of 450 total aligned reads displayed.

CCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCC  >  NC_014034/1395573‑1395773
                                                                                                    |                                                                                                      
ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                        >  1:6749829/1‑101 (MQ=255)
ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                        <  2:2712063/101‑1 (MQ=255)
 cccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGt                                                                                                       <  1:8377408/101‑1 (MQ=255)
  ccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTa                                                                                                      <  2:2489928/101‑1 (MQ=255)
   cccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAg                                                                                                     <  2:2111984/101‑1 (MQ=255)
      gcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcg                                                                                                  >  2:7370200/1‑101 (MQ=255)
      gcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcg                                                                                                  >  2:2282387/1‑101 (MQ=255)
         ggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcg                                                                                               >  2:6256647/1‑101 (MQ=255)
          gggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcgg                                                                                              >  1:4003085/1‑101 (MQ=255)
            gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.Gc                                                                                           <  2:2811561/101‑1 (MQ=255)
              gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATTTGCATGTGTTCGGGCG.GCAg                                                                                         >  2:3245016/1‑101 (MQ=37)
              gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAg                                                                                         >  2:8709075/1‑101 (MQ=255)
                ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGGG.GCAGtt                                                                                       >  2:3202177/1‑101 (MQ=255)
                ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGtt                                                                                       >  2:7731172/1‑101 (MQ=255)
                 gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTc                                                                                      <  2:1858858/101‑1 (MQ=255)
                  aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                     <  1:1006223/101‑1 (MQ=255)
                     gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gc                                                                                 >  1:855678/1‑101 (MQ=255)
                        accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGc                                                                              >  1:3307998/1‑101 (MQ=255)
                         ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCa                                                                             <  2:4986334/101‑1 (MQ=255)
                          caccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAg                                                                            >  2:6511051/1‑101 (MQ=255)
                            ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTc                                                                          <  2:7637537/101‑1 (MQ=255)
                             cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCg                                                                         <  1:6633202/101‑1 (MQ=255)
                              ttGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGc                                                                        >  1:1486275/1‑101 (MQ=255)
                                gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTc                                                                      >  2:9046997/1‑101 (MQ=255)
                                gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTc                                                                      <  1:4803976/101‑1 (MQ=255)
                                  ggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGTAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTccc                                                                    >  2:5225923/1‑101 (MQ=255)
                                   gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccc                                                                   <  2:7985955/101‑1 (MQ=255)
                                   gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccc                                                                   >  2:1022323/1‑101 (MQ=255)
                                      tGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                <  2:1236883/101‑1 (MQ=255)
                                      tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                <  2:5345495/101‑1 (MQ=255)
                                      tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCCGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                >  1:6253794/1‑101 (MQ=255)
                                       gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                               >  2:7488964/1‑101 (MQ=255)
                                       gTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                               <  2:2437878/101‑1 (MQ=255)
                                         cACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgg                                                             >  1:1731193/1‑101 (MQ=255)
                                         cACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GACGCAGTCGCTCCCCCCGCgg                                                             >  1:5642593/1‑101 (MQ=255)
                                         cACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCTGCATGCAGTCGGCGG.GCAGCTCTA.GCCGCAGTCGCTCCCGCCGCgg                                                             >  1:2979398/1‑101 (MQ=38)
                                            tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                          >  1:805534/1‑101 (MQ=255)
                                               cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGGCGGCGG.GCAGTTCGA.GCCGCAGTCGCTTTCCCCGCGGGGGCGt                                                       >  2:1092811/1‑101 (MQ=38)
                                                tggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGt                                                       <  1:7908246/91‑1 (MQ=2)
                                                gcgGGGCGAAGCTTCGCTCCCCCCGCGGGGTCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTg                                                      <  1:9047419/101‑1 (MQ=255)
                                                 ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTg                                                      >  2:4030451/10‑101 (MQ=2)
                                                 ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTg                                                      >  1:6473577/10‑101 (MQ=2)
                                                  gtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                     <  2:1471612/93‑1 (MQ=2)
                                                   gggCGAGGCGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  1:1140279/101‑1 (MQ=255)
                                                   gggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  1:3738050/101‑1 (MQ=255)
                                                    ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  1:7370524/101‑1 (MQ=255)
                                                    ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  2:5154487/101‑1 (MQ=255)
                                                    ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  2:8575674/101‑1 (MQ=255)
                                                    gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  2:4398267/7‑101 (MQ=2)
                                                     ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  2:2993034/96‑1 (MQ=2)
                                                      cGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  1:1905394/101‑1 (MQ=255)
                                                        ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               <  1:6909263/99‑1 (MQ=2)
                                                         aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                             >  2:8386340/1‑101 (MQ=255)
                                                          gCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                            <  2:7270363/101‑1 (MQ=255)
                                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCCGGAGGTAGTGGCCGG.GCAGTTCGA.GCGGGAGTGGCGCCCCCCGCGGGGGCGTGGGGCGAAACTg                                           >  2:4252254/1‑101 (MQ=18)
                                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                           <  2:1339611/101‑1 (MQ=255)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                        >  2:3718437/1‑101 (MQ=255)
                                                               gcgccccccGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                       <  1:718584/98‑1 (MQ=255)
                                                               gccccccccGCGGGGGGGGGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                       <  1:2322296/98‑1 (MQ=255)
                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTTGATCGCAACTGGTAt                                       >  1:8642442/1‑101 (MQ=255)
                                                               cctgcccccGCGGGGGGGTGGATCGAAACATCTGCATGTGGTCGGCGG.GCAGGTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                       <  2:609244/97‑1 (MQ=37)
                                                                cccccccgGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATg                                      <  2:8498000/99‑1 (MQ=255)
                                                                 cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                     <  1:5226092/100‑1 (MQ=255)
                                                                 cccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                     <  1:2479275/100‑1 (MQ=255)
                                                                    ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATc                                  <  1:5551317/101‑1 (MQ=255)
                                                                    ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATc                                  <  1:6584868/101‑1 (MQ=255)
                                                                     gccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCGGTGCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                 <  1:746269/100‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                 >  1:2678052/1‑101 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                 <  1:437804/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                 <  2:6674320/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                 <  1:4520389/101‑1 (MQ=255)
                                                                      ccGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTg                                <  1:2033584/101‑1 (MQ=255)
                                                                      ccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTg                                <  1:6138406/101‑1 (MQ=255)
                                                                        gcgGGGGCGTGGATCGAAACTTCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                              <  1:8494141/101‑1 (MQ=255)
                                                                         cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                             <  1:5083734/101‑1 (MQ=255)
                                                                          gggggCGTGGATCGAAACATCTGCATGTAGGCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                            <  1:3834646/101‑1 (MQ=255)
                                                                           ggggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                           >  2:5456337/1‑101 (MQ=255)
                                                                           ggggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                           <  2:5613232/101‑1 (MQ=255)
                                                                            gggCGTGGATCGAAACATCTGCATGTAGGCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGGATGGATCTGGCGCaa                          >  1:1572453/1‑101 (MQ=255)
                                                                              gCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                        <  1:945916/101‑1 (MQ=255)
                                                                              gCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                        <  1:2084392/101‑1 (MQ=255)
                                                                                gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCATTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                      <  2:5898783/101‑1 (MQ=255)
                                                                                gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                      >  2:6313985/1‑101 (MQ=255)
                                                                                gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                      >  1:3637647/1‑101 (MQ=255)
                                                                                  ggATCGAAACATCTGGATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTGTGGATCTGGCGCAAACGGaa                    >  1:8842894/1‑101 (MQ=255)
                                                                                  ggATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                    >  2:705893/1‑101 (MQ=255)
                                                                                   gATCGAAACACGTGCAGAGGGTGGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                   <  2:8854089/101‑1 (MQ=25)
                                                                                    aTCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                  >  2:4761205/1‑101 (MQ=255)
                                                                                        aaaCATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg              >  2:5681865/1‑101 (MQ=255)
                                                                                        aaaCATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg              >  2:7539837/1‑101 (MQ=255)
                                                                                          aCATCTGCAGGTAGTGGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtc            <  2:2031092/101‑1 (MQ=255)
                                                                                            cttggCATGTAGTCGGCGG.GCAGTTGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt          <  2:80515/97‑1 (MQ=255)
                                                                                            ctCTGCATGTGGCCGGCGG.GCGGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt          <  2:2240799/100‑1 (MQ=255)
                                                                                            aTCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt          >  2:6801328/1‑101 (MQ=255)
                                                                                              cTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg        >  1:7659338/1‑101 (MQ=255)
                                                                                               tGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc       >  1:2921958/1‑101 (MQ=255)
                                                                                                gCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGcc      >  1:284226/1‑100 (MQ=255)
                                                                                                gCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt      >  1:5940120/1‑101 (MQ=255)
                                                                                                 cATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc     >  2:1631048/1‑101 (MQ=255)
                                                                                                    gTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc  <  2:4530945/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
CCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCC  >  NC_014034/1395573‑1395773

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG