Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,741 C→A 16.2% intergenic (+553/‑378) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7410C→A16.2% 225.2 ‑1.6 ‑0.0523intergenic (+553/‑378)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (50/35):  ref base (223/215):  total (273/250)
Fisher's exact test strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 542 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCC  >  NC_014034/1395641‑1395841
                                                                                                     |                                                                                                          
ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATc                                                                                                            <  1:5551317/101‑1 (MQ=255)
ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATc                                                                                                            <  1:6584868/101‑1 (MQ=255)
      gggggCGTGGATCGAAACATCTGCAAGTAGGCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..gcgc                                                                                                    <  2:7526628/101‑1 (MQ=255)
       ggggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCa                                                                                                   >  2:2437669/1‑101 (MQ=255)
        gggCGTGGATCGAAACATCTGCATGTAGGCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGGATGGATCTG..GCGCaa                                                                                                  >  1:1572453/1‑101 (MQ=255)
         ggggTGGATCGAAACATCTGCATGTAGTCGGCTGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCaaa                                                                                                 <  2:6749558/98‑1 (MQ=255)
         ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCaaa                                                                                                 <  2:1021889/101‑1 (MQ=255)
         ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCaaa                                                                                                 <  1:8744175/101‑1 (MQ=255)
         ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCaaa                                                                                                 >  1:81524/1‑101 (MQ=255)
         ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCaaa                                                                                                 >  2:7380700/1‑101 (MQ=255)
          gCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAAc                                                                                                <  1:6296872/101‑1 (MQ=255)
           cGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACg                                                                                               <  2:3497480/101‑1 (MQ=255)
           cGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACg                                                                                               <  1:2565719/101‑1 (MQ=255)
            gTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACgg                                                                                              >  2:6313985/1‑101 (MQ=255)
               gATCGAAACACGTGCAGAGGGTGGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..aaa                                                                                         <  2:8854089/101‑1 (MQ=25)
                aTCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAg                                                                                        <  1:6748140/101‑1 (MQ=255)
                aTCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAg                                                                                        <  1:7489288/101‑1 (MQ=255)
                aTCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAg                                                                                        <  1:2038867/101‑1 (MQ=255)
                   gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCt                                                                                     >  1:1499670/1‑101 (MQ=255)
                   gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCt                                                                                     >  1:5123917/1‑101 (MQ=255)
                    aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g                                                                                   >  2:4446939/1‑101 (MQ=255)
                      aCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tc                                                                                >  1:5419411/1‑101 (MQ=255)
                         tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tcgtc                                                                             >  2:7667616/1‑101 (MQ=255)
                         tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGAAAACGG..AAAGTCT.g.tcgtc                                                                             >  2:2254865/1‑101 (MQ=255)
                          cTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.tcgtcg                                                                            >  1:7659338/1‑101 (MQ=255)
                            gCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                          >  2:2704956/1‑101 (MQ=255)
                              aTGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                        <  1:8883201/101‑1 (MQ=255)
                              aTGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                        <  1:5941947/101‑1 (MQ=255)
                              aTGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                        <  2:7953520/101‑1 (MQ=255)
                               tGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccc                                                                       <  1:913718/101‑1 (MQ=255)
                                gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                      >  2:6874540/1‑101 (MQ=255)
                                  aGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccccc                                                                    >  1:437297/1‑101 (MQ=255)
                                             aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCgcg                                                         >  1:7162967/1‑99 (MQ=255)
                                              gTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                        >  2:5496538/1‑101 (MQ=255)
                                                 caagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  1:1267601/94‑1 (MQ=2)
                                                 cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:1092829/101‑1 (MQ=255)
                                                 cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:7084986/101‑1 (MQ=255)
                                                 cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:4761355/101‑1 (MQ=255)
                                                  gggCCGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:5131107/99‑1 (MQ=255)
                                                  gAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:3855793/101‑1 (MQ=255)
                                                  aagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  1:287577/7‑101 (MQ=2)
                                                  aagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  2:3305846/7‑101 (MQ=2)
                                                   agattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  1:6913411/96‑1 (MQ=2)
                                                   aGCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  1:3412370/101‑1 (MQ=255)
                                                    gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:1417513/95‑1 (MQ=0)
                                                    gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:5279901/97‑1 (MQ=2)
                                                    gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  >  2:4861690/1‑101 (MQ=255)
                                                    gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:2641861/101‑1 (MQ=255)
                                                     attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:2180621/4‑101 (MQ=2)
                                                      ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  2:1799774/99‑1 (MQ=2)
                                                      ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   >  1:5752179/3‑101 (MQ=2)
                                                      gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:4165738/97‑1 (MQ=2)
                                                       tcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  >  1:1793170/2‑101 (MQ=2)
                                                       ccAGGCGCCCCCCCCGCGGGGGCGTGGATCGAAGCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGGGGGGGCGTGGATCGAAAcc                                               <  2:8642056/100‑1 (MQ=38)
                                                        tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                >  1:6352263/3‑101 (MQ=2)
                                                        tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                <  1:299392/99‑1 (MQ=2)
                                                        cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                 >  2:3148402/1‑101 (MQ=2)
                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAcc                                               >  1:7352713/1‑101 (MQ=2)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcac                                              <  1:2796310/101‑3 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                             <  2:5751946/101‑4 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                             <  2:939097/101‑4 (MQ=2)
                                                            cGCTCCCCCCGCAGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcgg                                          >  2:6699896/1‑101 (MQ=255)
                                                            cGCTCCCACCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                            <  2:1186990/101‑3 (MQ=2)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                            >  2:8329997/1‑97 (MQ=2)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                            <  1:4915336/101‑5 (MQ=2)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                            <  1:7900976/101‑5 (MQ=2)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                           >  2:532018/1‑98 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                          >  2:6976672/1‑95 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                          >  1:8352658/1‑95 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                         >  1:1870992/1‑96 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggcgg                                       >  2:4742426/1‑100 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                      <  1:4540226/101‑1 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                      >  2:6476349/1‑101 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.CCGTCGCTCCCACCGCGGGGGCGTGGATCGACACCTGCGGCGTg                                      >  2:9050991/1‑101 (MQ=255)
                                                                 cccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcg                                        >  1:3156675/1‑93 (MQ=2)
                                                                 cccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcg                                        >  2:6210854/1‑93 (MQ=2)
                                                                 cccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGa                                     <  1:3634598/101‑1 (MQ=255)
                                                                   cccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCC.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                                      <  2:7825424/101‑11 (MQ=2)
                                                                       cgGGGGCGTGGATCGAAACT.GGTAGGGATCTG..GCGCAAACGG..GAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                               >  2:4372518/1‑101 (MQ=255)
                                                                         ggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                             <  1:3725637/101‑1 (MQ=255)
                                                                         ggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                             <  2:5906091/101‑1 (MQ=255)
                                                                         ggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                             >  2:6070831/1‑101 (MQ=255)
                                                                              gTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                        <  1:2686942/101‑1 (MQ=255)
                                                                                  aTCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                    <  1:1012692/101‑1 (MQ=255)
                                                                                  aTCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                    <  2:6625740/101‑1 (MQ=255)
                                                                                   tCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                   <  1:8617715/101‑1 (MQ=255)
                                                                                    cGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGcc                  >  1:4220314/1‑101 (MQ=255)
                                                                                    cGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGcc                  <  2:8055995/101‑1 (MQ=255)
                                                                                     gAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCg                 >  1:2332773/1‑101 (MQ=255)
                                                                                      aaaCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGt                >  1:6140128/1‑101 (MQ=255)
                                                                                        aCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg              <  2:710818/101‑1 (MQ=255)
                                                                                          t.GGTATTGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc            <  2:2921860/101‑1 (MQ=255)
                                                                                          t.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc            <  2:1572406/101‑1 (MQ=255)
                                                                                            ggTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACg           >  2:5595285/1‑101 (MQ=255)
                                                                                            ggTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACg           >  2:5013921/1‑101 (MQ=255)
                                                                                            ggTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACg           >  2:1985232/1‑101 (MQ=255)
                                                                                              tATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc         >  1:2230903/1‑101 (MQ=255)
                                                                                               aTGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCg        >  2:369108/1‑101 (MQ=255)
                                                                                               aTGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCg        >  2:2833403/1‑101 (MQ=255)
                                                                                                tGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGc       >  1:6427008/1‑101 (MQ=255)
                                                                                                     |                                                                                                          
CCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCC  >  NC_014034/1395641‑1395841

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG