Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,839 C→A 10.9% intergenic (+651/‑280) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8390C→A10.9% 121.9 ‑1.3 ‑1.3545intergenic (+651/‑280)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/31):  ref base (272/211):  total (301/244)
Fisher's exact test strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.51e-02

Only 100 of 599 total aligned reads displayed.

ATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTC  >  NC_014034/1395739‑1395939
                                                                                                       |                                                                                                            
aTCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcc                                                                                                              >  2:7676564/1‑101 (MQ=255)
 tCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccc                                                                                                             >  2:6913591/1‑101 (MQ=255)
     gcgcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcg                                                                                                         >  2:8055461/1‑101 (MQ=255)
         aaaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                                                                                                     >  2:4701696/1‑101 (MQ=255)
         aaaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                                                                                                     >  1:2558939/1‑101 (MQ=255)
              gAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGt                                                                                                <  1:8225510/101‑1 (MQ=255)
              gAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGt                                                                                                <  1:3423946/101‑1 (MQ=255)
              gAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGt                                                                                                >  1:4412105/1‑101 (MQ=255)
               ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCgg                                                                                                >  1:3104804/10‑100 (MQ=0)
               ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCgg                                                                                                >  2:2626653/10‑100 (MQ=0)
               aaaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                                               <  2:3616515/101‑1 (MQ=255)
                aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.TCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                                              <  2:1893884/101‑1 (MQ=255)
                   tCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                                           <  1:2358964/101‑1 (MQ=255)
                   tCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                                           >  1:6300076/1‑101 (MQ=255)
                   tCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                                           >  2:1642099/1‑101 (MQ=255)
                   tCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                                           <  1:7778554/101‑1 (MQ=255)
                      gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                                        <  2:7832106/101‑1 (MQ=255)
                        cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                      >  1:6091341/1‑101 (MQ=255)
                        cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                      >  1:969588/1‑101 (MQ=255)
                        cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                      >  2:3409311/1‑101 (MQ=255)
                        cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                      <  2:6253555/101‑1 (MQ=255)
                           cgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                                                                                   >  2:8963417/1‑101 (MQ=255)
                            gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaagatc                                                                                  >  1:6205664/1‑96 (MQ=255)
                            gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                                                  >  1:5472150/1‑101 (MQ=255)
                             cTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg.c                                                                                >  1:5125249/1‑101 (MQ=255)
                               ccccccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcg.ccg                                                                              >  2:7172555/1‑101 (MQ=255)
                                 ccccGCGGGGGCGTGGATCGAAACCTGCG.GCGGGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCg.ccgc.c                                                                           >  2:3227267/1‑101 (MQ=255)
                                  cccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                          >  1:8786067/1‑101 (MQ=255)
                                   ccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                         >  1:7597107/1‑101 (MQ=255)
                                     gcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                       <  1:5014081/101‑1 (MQ=255)
                                       gggggCGTGGATCGAAACCTGCG.GCGTGATTTGCCAGTAATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCg.ccgc.ccgcctt                                                                     <  2:5693325/101‑7 (MQ=37)
                                       gggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGc                                                                     <  1:2773506/101‑1 (MQ=255)
                                        ggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCa                                                                    >  1:5835785/1‑101 (MQ=255)
                                          ggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                  >  2:142827/1‑101 (MQ=255)
                                           gCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcc                                                                 >  1:2144917/1‑101 (MQ=255)
                                           gCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcc                                                                 <  1:1088598/101‑1 (MQ=255)
                                            cGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                <  1:7996891/101‑1 (MQ=255)
                                             gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGGGGGGGTCGGAAGTTGG.CCCA.CTATCGAAGCCgg                                                               >  2:2920546/1‑101 (MQ=255)
                                             gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                               >  1:3762810/1‑101 (MQ=255)
                                             gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                               >  2:7455115/1‑101 (MQ=255)
                                             gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                               <  1:4785729/101‑1 (MQ=255)
                                             gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                               >  2:6403180/1‑101 (MQ=255)
                                              tGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...t                                                           <  1:2634323/101‑1 (MQ=255)
                                                      aaCCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgcc                                                   >  1:8991156/1‑101 (MQ=255)
                                                          tGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.gcg.c                                             >  2:3108342/1‑101 (MQ=255)
                                                          tGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.gcg.c                                             >  1:2975385/1‑101 (MQ=255)
                                                          tGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.gcg.c                                             >  1:126105/1‑101 (MQ=255)
                                                           gcg.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.Ca                                            <  1:7172859/101‑1 (MQ=255)
                                                           gcg.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.Ca                                            >  1:5935295/1‑101 (MQ=255)
                                                            cg.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.g                                          >  1:6633721/1‑101 (MQ=255)
                                                               gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTc                                        >  2:6137465/1‑101 (MQ=255)
                                                               gcgTGAGTTGCCAGTGATAAA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTc                                        <  2:6563097/101‑1 (MQ=255)
                                                               gcgTGAGTTGCCAGTCATAGA.CGCCGTCGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTc                                        <  2:4220196/101‑1 (MQ=255)
                                                                cgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCg                                       <  1:3721431/101‑1 (MQ=255)
                                                                cgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCg                                       >  2:81744/1‑101 (MQ=255)
                                                                cgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCg                                       <  2:5233996/101‑1 (MQ=255)
                                                                 gTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGc                                      >  2:3854064/1‑101 (MQ=255)
                                                                 gTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGc                                      <  2:486955/101‑1 (MQ=255)
                                                                    aGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGAGGATCGAAACTGAG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcc                                   >  1:845745/1‑101 (MQ=255)
                                                                     gTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTccc                                  >  1:4072680/1‑101 (MQ=255)
                                                                        gCCAGTGATAGG.CGCCGTGGA.CGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                               <  2:8806665/101‑1 (MQ=255)
                                                                           aGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgcg                            >  2:6681059/1‑101 (MQ=255)
                                                                             tGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCggg                          >  1:423853/1‑101 (MQ=255)
                                                                              gATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCgggg                         >  2:2404976/1‑101 (MQ=255)
                                                                                tAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGc                       <  1:6804938/101‑1 (MQ=255)
                                                                                 aGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...CCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                      >  2:2747556/1‑101 (MQ=255)
                                                                                  gA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                     <  1:563711/101‑1 (MQ=255)
                                                                                   a.CGCCGTGGA.TGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                    >  2:8283198/1‑101 (MQ=255)
                                                                                      gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                   <  2:5631704/91‑1 (MQ=9)
                                                                                       ccGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                 >  2:1216888/1‑101 (MQ=255)
                                                                                        ggcccggcgccGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CTGC.GTCTCGAAGACGGTCTTCTGGTGCC.GCC.GC.GTCGCTCCCCCCGCGGGGGCGTg                    >  2:4070325/11‑101 (MQ=2)
                                                                                        ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                    >  2:7064506/8‑101 (MQ=2)
                                                                                        caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                 >  1:1690911/9‑101 (MQ=9)
                                                                                        cGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                <  2:855665/101‑1 (MQ=255)
                                                                                         gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                   >  1:8631975/7‑101 (MQ=2)
                                                                                         gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg               <  1:6913876/101‑1 (MQ=255)
                                                                                         gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg               >  2:1767133/1‑101 (MQ=255)
                                                                                         aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                <  2:2151917/94‑1 (MQ=9)
                                                                                          cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGa                  <  2:5477712/96‑1 (MQ=0)
                                                                                          cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGa                  >  1:5590735/6‑101 (MQ=0)
                                                                                          acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg               <  2:7230290/95‑1 (MQ=9)
                                                                                          acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg               <  1:4879703/95‑1 (MQ=9)
                                                                                           ggA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa             >  2:6468597/1‑101 (MQ=255)
                                                                                           cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                <  2:2003215/97‑1 (MQ=2)
                                                                                            gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg               <  1:1003239/98‑1 (MQ=2)
                                                                                            ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa             >  1:6463146/5‑101 (MQ=9)
                                                                                             ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg               <  2:5409195/99‑1 (MQ=2)
                                                                                              ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa             >  1:8336635/2‑101 (MQ=2)
                                                                                              atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc           <  1:1456844/99‑1 (MQ=9)
                                                                                                 tctccccccccGCGGGGTCGTGGACCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGAGGGGGCGTGGATCGAAACGAt        <  2:4465042/96‑1 (MQ=38)
                                                                                                 tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt        <  1:737957/101‑1 (MQ=9)
                                                                                                 tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt        <  1:5263338/101‑1 (MQ=9)
                                                                                                 gcGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt        <  1:1995535/100‑1 (MQ=9)
                                                                                                 gcGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt        <  2:591697/100‑1 (MQ=255)
                                                                                                   gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc      <  2:1290315/101‑1 (MQ=9)
                                                                                                   gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc      <  2:8415453/101‑1 (MQ=9)
                                                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg     >  1:356836/1‑101 (MQ=255)
                                                                                                    cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg     >  1:6704049/1‑101 (MQ=9)
                                                                                                    cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg     >  1:4576094/1‑101 (MQ=9)
                                                                                                      ccccccGCGGGGGCGTGGATCGAAACTTCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt   <  2:5102342/101‑1 (MQ=255)
                                                                                                       |                                                                                                            
ATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTC  >  NC_014034/1395739‑1395939

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG