Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,865 G→C 6.5% intergenic (+677/‑254) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8650G→C 6.5% 77.8 0.0 ‑1.9583intergenic (+677/‑254)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/20):  ref base (259/284):  total (277/306)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.36e-02

Only 100 of 621 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGG  >  NC_014034/1395765‑1395965
                                                                                                    |                                                                                                            
       ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCg.ccgc.c                                                                                                     >  2:3595378/1‑101 (MQ=255)
        cccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                                                    <  2:7435890/101‑1 (MQ=255)
         ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                                                   <  1:7748388/101‑1 (MQ=255)
         ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                                                   >  1:7597107/1‑101 (MQ=255)
         ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCT.CCGC.CTa                                                                                                   >  2:2100441/1‑101 (MQ=255)
          cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                  <  1:4447066/101‑1 (MQ=255)
           gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                 <  1:5014081/101‑1 (MQ=255)
               gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                             <  2:16431/101‑1 (MQ=255)
                ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                                            <  1:4701845/101‑1 (MQ=255)
                 gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcc                                                                                           >  1:2144917/1‑101 (MQ=255)
                  cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                          <  2:4514164/101‑1 (MQ=255)
                   gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                         >  1:6104279/1‑101 (MQ=255)
                    tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...t                                                                                     <  2:5123748/101‑1 (MQ=255)
                    tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...t                                                                                     <  2:6955761/101‑1 (MQ=255)
                    tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...t                                                                                     >  2:6511961/1‑101 (MQ=255)
                     ggATCGAACCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                    <  1:4252370/101‑1 (MQ=255)
                     ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CGGC.CTATAGCATCCGG...Tc                                                                                    >  2:5423314/1‑101 (MQ=255)
                     ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                    >  1:644436/1‑101 (MQ=255)
                     ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                    >  2:1301202/1‑101 (MQ=255)
                        tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGTCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                 <  1:6266632/101‑1 (MQ=255)
                        tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                 >  1:1279011/1‑101 (MQ=255)
                         cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgc                                                                                >  2:8139903/1‑101 (MQ=255)
                         cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgc                                                                                >  2:1771778/1‑101 (MQ=255)
                         cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGAATCCGG...TCGAgc                                                                                >  1:8925272/1‑101 (MQ=255)
                           aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcgc                                                                              >  2:6442721/1‑101 (MQ=255)
                             aCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgcc.g                                                                           >  2:7683025/1‑101 (MQ=255)
                                 gcggggTGAGTCCCCAGTGATAGACGCCGTGGACCTCCCCCCCCCCGCGGGGCAGGGCATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.TCG.Ca                                                                      <  2:5973596/101‑1 (MQ=255)
                                  cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.g                                                                    >  1:6633721/1‑101 (MQ=255)
                                    gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTc                                                                  >  2:6137465/1‑101 (MQ=255)
                                        gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTc                                                              >  1:9063813/1‑101 (MQ=255)
                                          gTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTccc                                                            >  1:4072680/1‑101 (MQ=255)
                                             gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                                                         <  2:408524/101‑1 (MQ=255)
                                              cagatgctAGCCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcg                                                        <  1:2913843/94‑1 (MQ=38)
                                                  tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCggg                                                    >  2:8831390/1‑101 (MQ=255)
                                                  tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCggg                                                    >  2:7763331/1‑101 (MQ=255)
                                                   gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCgggg                                                   >  2:2404976/1‑101 (MQ=255)
                                                    aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCggggg                                                  <  1:705903/101‑1 (MQ=255)
                                                     tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGc                                                 <  1:3725089/101‑1 (MQ=255)
                                                      aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                >  1:832075/1‑101 (MQ=255)
                                                      aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                <  1:1186412/101‑1 (MQ=255)
                                                        aCGCCGTGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                              >  2:8283198/1‑101 (MQ=255)
                                                         cGCCGTGGACGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTgg                                             <  2:2959158/101‑1 (MQ=255)
                                                          gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGa                                            <  2:6275556/101‑1 (MQ=255)
                                                          ccaacctatgTCGCTCGCCCCGCGGGGGCGTGTATCGTAACTGTG.CCGG.TTCCCGGAGAC.G...CGGGGCCCC.TCG.CACTTCGCTCCCCCCGCGGGGGCGTGGa                                            <  2:3169353/92‑1 (MQ=2)
                                                          ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                                            <  2:6630180/92‑1 (MQ=9)
                                                           ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                              >  2:7078100/8‑101 (MQ=2)
                                                           ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:6718845/101‑1 (MQ=255)
                                                           ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:5636510/101‑1 (MQ=255)
                                                           ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           >  2:1216888/1‑101 (MQ=255)
                                                           ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           >  2:7210822/1‑101 (MQ=255)
                                                           ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           >  2:4928236/1‑101 (MQ=255)
                                                           caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                           >  1:1690911/9‑101 (MQ=9)
                                                             gTGGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:7343877/101‑1 (MQ=255)
                                                             gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:2568107/101‑1 (MQ=255)
                                                              cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  1:5932566/97‑1 (MQ=2)
                                                              cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  1:4870085/97‑1 (MQ=2)
                                                              cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  2:8511203/6‑101 (MQ=9)
                                                              ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           >  2:5538293/5‑101 (MQ=2)
                                                               gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:1003239/98‑1 (MQ=2)
                                                               ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  1:8399746/5‑101 (MQ=9)
                                                                ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:2694983/3‑101 (MQ=2)
                                                                 atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     >  1:5138792/3‑101 (MQ=9)
                                                                 atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     <  2:3911311/99‑1 (MQ=9)
                                                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  2:1955237/101‑1 (MQ=2)
                                                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                    >  2:5284783/1‑101 (MQ=255)
                                                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  1:2515867/1‑101 (MQ=2)
                                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GG.GTCGCTCCCCCCGCGGGGGCGCGGATCGaac                                      >  1:2981722/1‑100 (MQ=2)
                                                                   ctCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                   <  2:5835550/100‑1 (MQ=255)
                                                                     cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACAATg                                 >  1:5297549/1‑101 (MQ=9)
                                                                      gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                <  2:8997320/101‑1 (MQ=9)
                                                                      gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                <  1:5397039/101‑1 (MQ=9)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                               >  1:356836/1‑101 (MQ=255)
                                                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                               >  1:4576094/1‑101 (MQ=9)
                                                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATAGatcggaagag                               >  1:8556946/1‑92 (MQ=9)
                                                                        tcaccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                              >  2:8039788/4‑100 (MQ=9)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                              >  1:7898144/1‑101 (MQ=255)
                                                                         caccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCCCCCCCCGCGGGGGCGAGGATCGAAACAATGCggc                             >  2:8590016/3‑99 (MQ=9)
                                                                          cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                            <  2:2724771/101‑1 (MQ=255)
                                                                           ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                           <  1:995089/101‑1 (MQ=255)
                                                                            cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                          <  2:1486228/101‑1 (MQ=255)
                                                                            cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                          <  1:6910944/101‑1 (MQ=255)
                                                                             ccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgg                         <  2:228851/101‑7 (MQ=2)
                                                                             ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                            <  1:1465877/101‑10 (MQ=2)
                                                                               gcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGc                       >  1:8690462/1‑101 (MQ=255)
                                                                                cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                      <  1:81110/101‑10 (MQ=2)
                                                                                cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                      >  2:3355327/1‑92 (MQ=2)
                                                                                 gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                     >  2:635560/1‑101 (MQ=255)
                                                                                 gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                     >  1:5560317/1‑101 (MQ=255)
                                                                                  ggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACGATGCGTTCATGGCCTa                    >  1:5632861/1‑101 (MQ=255)
                                                                                  ggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                    <  1:7444097/101‑1 (MQ=255)
                                                                                  ggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                    <  1:3854173/101‑1 (MQ=255)
                                                                                   gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                   <  1:236182/101‑1 (MQ=255)
                                                                                   gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                   <  2:6681486/101‑1 (MQ=255)
                                                                                   gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                   <  2:4786632/101‑1 (MQ=255)
                                                                                   gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                   <  2:4351099/101‑1 (MQ=255)
                                                                                       gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg               <  2:3053753/101‑1 (MQ=255)
                                                                                        tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg              >  1:6789852/1‑101 (MQ=255)
                                                                                        tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGCTCATGGCCTACGCCgg              >  1:1973480/1‑101 (MQ=255)
                                                                                                aaCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa      <  1:3374176/101‑1 (MQ=255)
                                                                                                  cTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt    <  1:3134720/101‑1 (MQ=255)
                                                                                                    |                                                                                                            
TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGG  >  NC_014034/1395765‑1395965

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG