Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,871 C→G 17.6% intergenic (+683/‑248) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8710C→G17.6% 259.6 ‑0.2 ‑0.1575intergenic (+683/‑248)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (46/55):  ref base (222/252):  total (268/307)
Fisher's exact test strand distribution p-value = 8.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.60e-01

Only 100 of 610 total aligned reads displayed.

CCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTC  >  NC_014034/1395771‑1395971
                                                                                                     |                                                                                                            
  cccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                                                           >  1:8786067/1‑101 (MQ=255)
  cccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                                                           <  2:6299836/101‑1 (MQ=255)
    cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                         <  1:6874812/101‑1 (MQ=255)
    cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                         <  2:3326531/101‑1 (MQ=255)
    cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                         <  1:3567342/101‑1 (MQ=255)
     gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                        >  2:1100724/1‑101 (MQ=255)
     gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                        <  1:6700164/101‑1 (MQ=255)
       gggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGc                                                                                                      >  2:6218048/1‑101 (MQ=255)
        ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCa                                                                                                     <  2:7162663/101‑1 (MQ=255)
         gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                    >  1:4465177/1‑101 (MQ=255)
         gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                    >  2:6641853/1‑101 (MQ=255)
          ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                                                   >  2:142827/1‑101 (MQ=255)
          ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                                                   <  1:8231073/101‑1 (MQ=255)
           gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc.c                                                                                                 <  1:7381024/101‑1 (MQ=255)
            cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cg                                                                                                <  2:4514164/101‑1 (MQ=255)
            cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cg                                                                                                >  2:236179/1‑101 (MQ=255)
            cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cg                                                                                                <  2:1702010/101‑1 (MQ=255)
            cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cg                                                                                                >  1:8519928/1‑101 (MQ=255)
            cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cg                                                                                                <  1:7996891/101‑1 (MQ=255)
            cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cg                                                                                                >  2:2051880/1‑101 (MQ=255)
            agTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cg                                                                                                <  1:2097728/100‑1 (MQ=255)
             gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cgg                                                                                               >  2:6403180/1‑101 (MQ=255)
             gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cgg                                                                                               >  1:6104279/1‑101 (MQ=255)
             gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cgg                                                                                               <  1:4785729/101‑1 (MQ=255)
             gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cgg                                                                                               <  1:7192468/101‑1 (MQ=255)
             gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cgg                                                                                               >  1:3762810/1‑101 (MQ=255)
              tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...t                                                                                           <  1:2634323/101‑1 (MQ=255)
               ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...Tc                                                                                          <  2:1569560/101‑1 (MQ=255)
                  tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGTCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAg                                                                                       <  1:6266632/101‑1 (MQ=255)
                  tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAg                                                                                       >  1:1279011/1‑101 (MQ=255)
                    gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgcg                                                                                     >  2:5683986/1‑101 (MQ=255)
                      aaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGcgcc                                                                                   >  1:8991156/1‑101 (MQ=255)
                             ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.Gt                                                                         <  1:1956741/101‑1 (MQ=255)
                              gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTc                                                                        >  2:5894344/1‑101 (MQ=255)
                              gcgTGAGTTGCCAGTGATAAACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTc                                                                        <  2:6563097/101‑1 (MQ=255)
                               cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                       >  1:8647933/1‑101 (MQ=255)
                               cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                       <  2:5233996/101‑1 (MQ=255)
                               cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                       <  1:3721431/101‑1 (MQ=255)
                               cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                       >  2:7963118/1‑101 (MQ=255)
                               cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                       >  2:2698991/1‑101 (MQ=255)
                                gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGc                                                                      <  2:486955/101‑1 (MQ=255)
                                gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGc                                                                      >  2:3854064/1‑101 (MQ=255)
                                 tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCt                                                                     >  2:6776599/1‑101 (MQ=255)
                                   aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcc                                                                   >  2:3134621/1‑101 (MQ=255)
                                    gTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTccc                                                                  <  2:215881/101‑1 (MQ=255)
                                     ttGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcccc                                                                 <  1:4196794/101‑1 (MQ=255)
                                       gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                                                               >  1:6588591/1‑101 (MQ=255)
                                         cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgc                                                             >  1:5901632/1‑101 (MQ=255)
                                          aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgcg                                                            >  2:1271348/1‑101 (MQ=255)
                                                aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                      <  1:1186412/101‑1 (MQ=255)
                                                aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GGCGCTCCCCCCGCGGGGGCg                                                      >  2:8822694/1‑101 (MQ=255)
                                                 gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                     <  1:6737475/101‑1 (MQ=255)
                                                  aCGCCGTGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                    >  2:8283198/1‑101 (MQ=255)
                                                   gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACA.CCGG.GGCTCTGATCACGG...T.TTGGGAC.G.G..A.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  1:224643/91‑1 (MQ=2)
                                                   cGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTgg                                                   <  2:7716835/101‑1 (MQ=255)
                                                    cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGt                                                     >  1:5501247/9‑101 (MQ=2)
                                                     ggcccggcgccGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CTGC.GTCTCGAAGA.CGGTCTTCTGGTGCC.GCC.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                    >  2:4070325/11‑101 (MQ=2)
                                                     caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  1:5349325/93‑1 (MQ=9)
                                                      cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:5016146/101‑1 (MQ=255)
                                                      aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                >  1:4614776/8‑101 (MQ=9)
                                                       cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  1:1787703/96‑1 (MQ=0)
                                                       cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  2:5477712/96‑1 (MQ=0)
                                                       acctatgTCGCTCGCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:6967103/95‑1 (MQ=9)
                                                       acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:4879703/95‑1 (MQ=9)
                                                         ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  2:6468597/1‑101 (MQ=255)
                                                         gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:1003239/98‑1 (MQ=2)
                                                         cggcctCGCTCCCCCGCGGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  2:7357145/96‑1 (MQ=2)
                                                         cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  2:3579659/98‑1 (MQ=2)
                                                         cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:3355431/98‑1 (MQ=2)
                                                         cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:9151687/98‑1 (MQ=2)
                                                         cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:8340378/98‑1 (MQ=2)
                                                          ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:3102999/99‑1 (MQ=2)
                                                          ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  2:5409195/99‑1 (MQ=2)
                                                          ggcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:4273423/99‑1 (MQ=2)
                                                           ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  2:6129773/100‑1 (MQ=2)
                                                           aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           >  2:4307400/1‑101 (MQ=255)
                                                            tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                          <  1:3919646/100‑2 (MQ=9)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                         >  2:1867321/1‑101 (MQ=255)
                                                                gttgcggcggcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                      >  2:5622341/10‑101 (MQ=2)
                                                                gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                      <  2:8997320/101‑1 (MQ=9)
                                                                   ccccccGCGGGGGCGTGGATCGAAACTTCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                   <  2:5102342/101‑1 (MQ=255)
                                                                   caccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                   >  2:8131311/3‑99 (MQ=9)
                                                                    accccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                  >  2:8566572/2‑98 (MQ=9)
                                                                     ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                 <  2:2558866/101‑1 (MQ=255)
                                                                      cccGGGGGGGCGGGCATCGAAACTGCG.CCGC.CTATGGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                <  2:2850320/101‑1 (MQ=38)
                                                                       ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                               <  1:517080/101‑1 (MQ=255)
                                                                       ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                  <  1:1465877/101‑10 (MQ=2)
                                                                        cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                              <  1:7560309/101‑1 (MQ=255)
                                                                           gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                           <  2:3004023/101‑11 (MQ=2)
                                                                           gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                           <  1:2833501/101‑1 (MQ=255)
                                                                           gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                           >  1:2461093/1‑101 (MQ=255)
                                                                            ggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACGATGCGTTCATGGCCTa                          >  1:5632861/1‑101 (MQ=255)
                                                                             gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                         <  1:7676890/101‑1 (MQ=255)
                                                                                    gATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                  <  2:700419/101‑1 (MQ=255)
                                                                                       cGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt               >  1:2978713/1‑101 (MQ=255)
                                                                                       cGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt               >  2:8506744/1‑101 (MQ=255)
                                                                                           aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAATCGATGCGTTCATGGCCTACGTCGGGCAGTGGAc           <  1:6442970/101‑1 (MQ=255)
                                                                                            cTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt          >  2:7656308/1‑101 (MQ=255)
                                                                                                     c.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc  <  2:474025/101‑1 (MQ=255)
                                                                                                     c.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc  >  1:7877931/1‑101 (MQ=255)
                                                                                                     |                                                                                                            
CCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTC  >  NC_014034/1395771‑1395971

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG