Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,881 C→A 24.4% intergenic (+693/‑238) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8810C→A24.4% 0.0 ‑0.7 0.0569intergenic (+693/‑238)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (64/74):  ref base (210/211):  total (276/293)
Fisher's exact test strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 601 total aligned reads displayed.

GGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGG  >  NC_014034/1395781‑1395981
                                                                                                      |                                                                                                             
 gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcc                                                                                                              <  1:9092110/101‑1 (MQ=255)
  cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                                             >  1:1153850/1‑101 (MQ=255)
  cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                                             >  2:236179/1‑101 (MQ=255)
   gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGGTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                                            >  1:8200155/1‑101 (MQ=255)
   gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                                            <  1:4785729/101‑1 (MQ=255)
   gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                                            >  1:3762810/1‑101 (MQ=255)
    tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...t                                                                                                        >  2:6511961/1‑101 (MQ=255)
     ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                                       >  2:4404649/1‑101 (MQ=255)
      gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..g                                                                                                    <  1:2217169/101‑1 (MQ=255)
      gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..g                                                                                                    <  2:2603834/101‑1 (MQ=255)
         cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAg.c                                                                                                >  2:8139903/1‑101 (MQ=255)
         cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAg.c                                                                                                >  2:5581224/1‑101 (MQ=255)
         cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...CC..GAg.c                                                                                                >  2:5468707/1‑101 (MQ=255)
          gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAg.cg                                                                                               >  2:5683986/1‑101 (MQ=255)
           aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAg.cgc                                                                                              >  2:6442721/1‑101 (MQ=255)
             aCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.cgcc.g                                                                                           >  2:7683025/1‑101 (MQ=255)
                     cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCg                                                                                 <  2:5233996/101‑1 (MQ=255)
                         aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTcc                                                                             >  2:6761381/1‑101 (MQ=255)
                         aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTcc                                                                             >  1:7640640/1‑101 (MQ=255)
                          gTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTccc                                                                            <  2:215881/101‑1 (MQ=255)
                           ttGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTcccc                                                                           <  1:4196794/101‑1 (MQ=255)
                              ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCcg                                                                        <  2:3773256/101‑1 (MQ=255)
                                  tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCggg                                                                    >  2:8831390/1‑101 (MQ=255)
                                  tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCggg                                                                    >  2:7763331/1‑101 (MQ=255)
                                    aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCggggg                                                                  >  2:7974560/1‑101 (MQ=255)
                                     tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGc                                                                 <  1:6804938/101‑1 (MQ=255)
                                      aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...CC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                >  2:2747556/1‑101 (MQ=255)
                                           caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTGCCCCCGCGGGGGCGTGGAt                                                           >  2:1003221/9‑101 (MQ=9)
                                           caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  2:8312240/93‑1 (MQ=9)
                                           caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  1:5349325/93‑1 (MQ=9)
                                            cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:5016146/101‑1 (MQ=255)
                                            cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGGGGATc                                                          >  2:4206457/1‑101 (MQ=255)
                                            aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:3694693/94‑1 (MQ=9)
                                            aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  1:4614776/8‑101 (MQ=9)
                                            aacctatgTCGCGCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  2:1233093/94‑1 (MQ=9)
                                             gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:6913876/101‑1 (MQ=255)
                                             cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  1:1787703/96‑1 (MQ=0)
                                             cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                            >  1:602646/6‑101 (MQ=0)
                                             acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:4879703/95‑1 (MQ=9)
                                              cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TC..GAG.CGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  2:2003215/97‑1 (MQ=2)
                                              cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  2:8511203/6‑101 (MQ=9)
                                               ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  1:6726806/1‑101 (MQ=255)
                                               gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TC..GAG.CGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:1003239/98‑1 (MQ=2)
                                               ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  1:6463146/5‑101 (MQ=9)
                                               cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:2037097/98‑1 (MQ=2)
                                                ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:3102999/99‑1 (MQ=2)
                                                ggcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:4273423/99‑1 (MQ=2)
                                                gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  1:6224343/101‑1 (MQ=255)
                                                cccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TC..GAG.CGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  1:2657028/3‑101 (MQ=2)
                                                 atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATccacac                                                     >  2:1340075/3‑96 (MQ=9)
                                                  tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                    <  1:3919646/100‑2 (MQ=9)
                                                  ggTCGCGCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TCCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                    <  1:736641/100‑2 (MQ=2)
                                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  2:6255251/101‑1 (MQ=2)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                   >  2:3092871/1‑101 (MQ=255)
                                                   gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaa                                                   >  2:5382293/1‑99 (MQ=9)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                  >  2:6957781/1‑101 (MQ=255)
                                                    tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                  <  1:5887491/101‑1 (MQ=9)
                                                    gcGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                  <  2:7132211/100‑1 (MQ=9)
                                                    gcGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                  <  2:591697/100‑1 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                 >  2:295976/1‑101 (MQ=255)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                >  1:3477329/1‑101 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                               >  1:5499652/1‑101 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGG...TCTTCTGTTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGGGTGGGTCGagcaccc                                                  >  1:8942870/1‑95 (MQ=0)
                                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACAATGCg                                               >  2:4035197/1‑101 (MQ=9)
                                                        tcaccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                              <  2:3487612/98‑2 (MQ=9)
                                                        tCCGCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGACGTGGATCGAAACGATGCGt                                              >  1:5619794/1‑101 (MQ=255)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                              >  2:1693938/1‑101 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACTTCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                             <  2:5102342/101‑1 (MQ=255)
                                                         caccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                             >  2:2464150/3‑99 (MQ=9)
                                                         caccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCCCCCCCCGCGGGGGCGAGGATCGAAACAATGCggc                                             >  2:8590016/3‑99 (MQ=9)
                                                          accccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                            >  1:8700168/2‑98 (MQ=9)
                                                           ccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATCCggcgc                                           >  2:4571040/1‑97 (MQ=9)
                                                           ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                           <  2:8999403/101‑1 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                          <  1:7455439/101‑1 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                          <  2:1486228/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                         <  2:4900972/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                         <  1:517080/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                         <  2:3219003/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                         <  1:2866039/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC..TGG.TGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                            <  1:1465877/101‑10 (MQ=2)
                                                              cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                        <  1:8333774/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG..GGG.C.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                      >  1:6639198/1‑92 (MQ=2)
                                                                cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                      <  2:6633461/101‑1 (MQ=255)
                                                                 gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                     >  2:635560/1‑101 (MQ=255)
                                                                 gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                     <  1:7974900/101‑1 (MQ=255)
                                                                 gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                     >  1:2461093/1‑101 (MQ=255)
                                                                 gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                     <  2:7241347/101‑1 (MQ=255)
                                                                   gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                   <  2:4786632/101‑1 (MQ=255)
                                                                   gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                   >  1:8416052/1‑101 (MQ=255)
                                                                       gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                               <  1:7156067/101‑1 (MQ=255)
                                                                         ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                             >  2:612991/1‑101 (MQ=255)
                                                                         ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                             <  1:784391/101‑1 (MQ=255)
                                                                           aTCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCa                           >  1:8596363/1‑101 (MQ=255)
                                                                             cGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                         >  1:2978713/1‑101 (MQ=255)
                                                                               aaaCTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                       >  1:4457574/1‑101 (MQ=255)
                                                                                          gc.cTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt             >  2:1850680/1‑101 (MQ=255)
                                                                                          gc.cTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt             <  1:6361454/101‑1 (MQ=255)
                                                                                              tATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc          >  1:1903090/1‑101 (MQ=255)
                                                                                                   cATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgc     >  1:1266537/1‑101 (MQ=255)
                                                                                                    aTCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg    >  1:3329256/1‑101 (MQ=255)
                                                                                                      |                                                                                                             
GGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC..GAG.CGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGG  >  NC_014034/1395781‑1395981

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG