Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,931 G→A 11.2% intergenic (+743/‑188) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,9310G→A11.2% 127.1 ‑0.6 ‑0.1471intergenic (+743/‑188)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (23/29):  ref base (200/206):  total (225/246)
Fisher's exact test strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.64e-01

Only 100 of 488 total aligned reads displayed.

CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGC  >  NC_014034/1395831‑1396031
                                                                                                     |                                                                                                    
cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                                                      >  2:5284783/1‑101 (MQ=255)
  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                                                                    >  2:6957781/1‑101 (MQ=255)
  gcGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                                                                    <  2:4120838/100‑1 (MQ=255)
  gcGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                                                                    <  2:591697/100‑1 (MQ=255)
   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                                                                   <  1:4256157/101‑1 (MQ=9)
   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                                                                   >  2:295976/1‑101 (MQ=255)
    gttgcggcggcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                                                                                  >  2:5622341/10‑101 (MQ=2)
      cccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                                                                                <  2:5419226/100‑1 (MQ=255)
       ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTNGATCGAAACGATGCGtt                                                                                               >  2:1523740/1‑101 (MQ=255)
       caccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                                                                               >  2:2464150/3‑99 (MQ=9)
        accccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                                                                              >  2:8566572/2‑98 (MQ=9)
         ccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATCCggcgc                                                                                             >  2:4571040/1‑97 (MQ=9)
         ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                             >  1:9158381/1‑101 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                                                                                            >  2:5106172/1‑96 (MQ=9)
           ccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgg                                                                                           <  2:228851/101‑7 (MQ=2)
           ccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgg                                                                                           <  2:7913438/101‑7 (MQ=2)
           ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                                                                           <  1:2866039/101‑1 (MQ=255)
            cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                          <  1:8333774/101‑1 (MQ=255)
             gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                                                         <  1:4600242/101‑9 (MQ=2)
              cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                                                                        <  1:81110/101‑10 (MQ=2)
              cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                                                        <  2:6633461/101‑1 (MQ=255)
               gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                                                                       <  2:9095494/101‑11 (MQ=2)
               gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                       >  2:1968291/1‑101 (MQ=255)
               gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                       <  2:7774503/101‑1 (MQ=255)
               gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                       >  1:3905723/1‑101 (MQ=255)
                ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACGATGCGTTCATGGCCTa                                                                                      >  1:5632861/1‑101 (MQ=255)
                ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                                                                      >  1:4401505/1‑101 (MQ=255)
                ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                                                                      <  1:3854173/101‑1 (MQ=255)
                ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                                                                      <  1:7081460/101‑1 (MQ=255)
                 gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                     <  1:3189064/101‑1 (MQ=255)
                 gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                     >  1:8806101/1‑101 (MQ=255)
                   gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                                                                   <  2:2368536/101‑1 (MQ=255)
                     gTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                                                                 >  2:6313615/1‑101 (MQ=255)
                     gTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                                                                 >  1:7419266/1‑101 (MQ=255)
                      tGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                                                                >  2:6473552/1‑101 (MQ=255)
                       ggATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                                                               >  1:7951468/1‑101 (MQ=255)
                         aTCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCa                                                                             >  1:8596363/1‑101 (MQ=255)
                            gAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                                                                          <  1:30869/101‑1 (MQ=255)
                             aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                                                         >  2:639558/1‑101 (MQ=255)
                                cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                                                      <  2:6091104/101‑1 (MQ=255)
                                  gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                    >  1:4102075/1‑101 (MQ=255)
                                  gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                    <  2:7138080/101‑1 (MQ=255)
                                   cgccgcCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                   <  1:1812374/101‑1 (MQ=255)
                                       gccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                                                               >  1:495628/1‑101 (MQ=255)
                                          tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                            >  1:1903090/1‑101 (MQ=255)
                                           atctgacccGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                                                           <  2:3482469/94‑1 (MQ=255)
                                            tCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc                                                          <  1:5875532/101‑1 (MQ=255)
                                             cGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                         <  1:5437816/101‑1 (MQ=255)
                                             cGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                         <  1:639568/101‑1 (MQ=255)
                                              gCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                                                        >  1:5982589/1‑101 (MQ=255)
                                               cATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgc                                                       >  1:1266537/1‑101 (MQ=255)
                                               cATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgc                                                       >  2:121855/1‑101 (MQ=255)
                                                aTCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg                                                      >  1:1040732/1‑101 (MQ=255)
                                                     gTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                 <  1:5541052/101‑1 (MQ=255)
                                                       cGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGGGGGGATCGAAACGATGCGTTCATGCCCTACGCCGGGCAGTGGCCTGGCGGCTCCCCCCCCgggggggg                                               >  1:3668264/1‑98 (MQ=25)
                                                        gAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg                                              <  1:2088990/101‑1 (MQ=255)
                                                         aGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg                                             <  1:6723351/101‑1 (MQ=255)
                                                           cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCACTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:6473801/101‑1 (MQ=255)
                                                            gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  1:3130363/101‑1 (MQ=255)
                                                             gcccgcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:3797025/98‑1 (MQ=255)
                                                             ccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:6218287/101‑1 (MQ=255)
                                                             ccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:1015367/101‑1 (MQ=255)
                                                             ccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:7552801/1‑101 (MQ=255)
                                                             ccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:1731140/101‑1 (MQ=255)
                                                               gcgcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  1:847792/101‑1 (MQ=255)
                                                               caggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  2:6697061/95‑1 (MQ=2)
                                                                   a.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                   >  2:8914290/1‑99 (MQ=255)
                                                                   a.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTc                                   <  2:8361505/101‑1 (MQ=255)
                                                                   a.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTc                                   >  2:7614076/1‑101 (MQ=255)
                                                                     gacGCACCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCg                                  <  2:845732/99‑1 (MQ=255)
                                                                      cggtcaccaccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGc                                 >  2:737943/10‑101 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGccc                               >  1:3554587/1‑101 (MQ=255)
                                                                          tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                             >  1:238278/1‑101 (MQ=255)
                                                                          cccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                             <  1:7667943/100‑1 (MQ=255)
                                                                           ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                            >  2:269554/1‑101 (MQ=255)
                                                                            cccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGa                           <  2:3573698/101‑1 (MQ=255)
                                                                               ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                        <  2:4401379/101‑1 (MQ=255)
                                                                                  cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgcg                     >  1:9054055/1‑101 (MQ=255)
                                                                                  cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgcg                     >  1:1387160/1‑101 (MQ=255)
                                                                                   gggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCgcgc                    >  1:4703419/1‑101 (MQ=255)
                                                                                    ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                   >  1:3362449/1‑101 (MQ=255)
                                                                                     gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                  <  1:5225646/101‑1 (MQ=255)
                                                                                       gCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGc                <  1:2704737/101‑1 (MQ=255)
                                                                                        cGTGGATCGAAACGATGCGTCCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTGGcc               <  1:7211126/101‑1 (MQ=255)
                                                                                         gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATTCGCCCTTGCCt              >  2:4893192/1‑101 (MQ=255)
                                                                                         gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt              >  1:2593630/1‑101 (MQ=255)
                                                                                         gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt              >  1:5284971/1‑101 (MQ=255)
                                                                                         gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt              >  1:7783928/1‑101 (MQ=255)
                                                                                           ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa            >  2:6502908/1‑101 (MQ=255)
                                                                                           ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa            <  1:6543053/101‑1 (MQ=255)
                                                                                             aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg          >  2:8192610/1‑101 (MQ=255)
                                                                                                gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt       <  2:8111220/101‑1 (MQ=255)
                                                                                                 aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg      >  2:8745815/1‑101 (MQ=255)
                                                                                                  aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc     >  2:289747/1‑101 (MQ=255)
                                                                                                  aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc     >  2:4312820/1‑101 (MQ=255)
                                                                                                   aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc    <  1:1693985/101‑1 (MQ=255)
                                                                                                   aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc    <  2:4965100/101‑1 (MQ=255)
                                                                                                    cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg   >  2:3110708/1‑101 (MQ=255)
                                                                                                    cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcg   >  1:6809462/1‑101 (MQ=255)
                                                                                                     gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgc  >  2:6268938/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGC  >  NC_014034/1395831‑1396031

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG