Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,000 C→G 15.8% intergenic (+812/‑119) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0000C→G15.8% 197.6 ‑0.8 ‑0.3500intergenic (+812/‑119)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (37/42):  ref base (217/204):  total (254/246)
Fisher's exact test strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.78e-01

Only 100 of 530 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGT  >  NC_014034/1395900‑1396100
                                                                                                     |                                                                                                        
cggtcaccaccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGc                                                                                                          >  2:737943/10‑101 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGccc                                                                                                        <  1:1130103/101‑1 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGccc                                                                                                        <  2:7897811/101‑1 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCt                                                                                                       <  2:8415689/101‑1 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                                                                                      >  2:7525314/1‑101 (MQ=255)
    cccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                                                                                      <  1:7667943/100‑1 (MQ=255)
    cccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTc                                                                                                      <  2:293056/100‑1 (MQ=255)
        cccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATc                                                                                                  <  2:8805696/101‑1 (MQ=255)
         ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATcc                                                                                                 <  2:2677970/101‑1 (MQ=255)
         ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATcc                                                                                                 >  2:6502235/1‑101 (MQ=255)
           gcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCcgc                                                                                               >  1:5892491/1‑101 (MQ=255)
             gggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCgggc                                                                                             >  1:4006525/1‑101 (MQ=255)
             gggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCgcgc                                                                                             >  2:6930532/1‑101 (MQ=255)
             gggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCgcgc                                                                                             >  1:4703419/1‑101 (MQ=255)
              ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCt                                                                                            >  1:2898355/1‑101 (MQ=255)
              ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCt                                                                                            >  1:3362449/1‑101 (MQ=255)
                 gCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGc                                                                                         >  2:228622/1‑101 (MQ=255)
                  cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGcc                                                                                        >  1:9124645/1‑101 (MQ=255)
                  cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGcc                                                                                        <  1:6080178/101‑1 (MQ=255)
                  cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGcc                                                                                        >  2:7727881/1‑101 (MQ=255)
                   gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                                                                                       >  1:5284971/1‑101 (MQ=255)
                     ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                                                                                     <  1:857279/101‑1 (MQ=255)
                     ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                                                                                     >  2:2016841/1‑101 (MQ=255)
                      gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                                                                    <  1:6761652/101‑1 (MQ=255)
                      gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                                                                    <  1:4885652/101‑1 (MQ=255)
                          gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                                                                <  1:6403429/101‑1 (MQ=255)
                          gAAACGATGCGTTCATGGCCTACGTCGGCCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                                                                >  1:6515640/1‑101 (MQ=255)
                            aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.Gc                                                                             >  2:4312820/1‑101 (MQ=255)
                             aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.Gc.c                                                                           <  2:5284787/101‑1 (MQ=255)
                              cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.cg                                                                          <  2:5935060/101‑1 (MQ=255)
                                  gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTc                                                                    <  1:2051942/101‑1 (MQ=255)
                                   cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCg                                                                   >  1:7296722/1‑101 (MQ=255)
                                     ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCt                                                                 >  2:3124487/1‑101 (MQ=255)
                                       cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTcc                                                               <  2:4101956/101‑1 (MQ=255)
                                        aTGACCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTGACGCCT.GC.CGC..GTCGCTccc                                                              >  1:7227486/1‑101 (MQ=255)
                                          ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTccccc                                                            >  1:2623788/1‑101 (MQ=255)
                                             cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCcgc                                                         <  2:4645393/101‑1 (MQ=255)
                                                cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCggg                                                      >  2:4053518/1‑101 (MQ=255)
                                                     ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCgg                                                 >  2:3649714/1‑100 (MQ=255)
                                                     ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                 <  1:4966214/101‑1 (MQ=255)
                                                     ggCAGTGGACTGGTCGCTCCCCCCCCGGGGGCGGGGGACCGAAACCCCCCCTCG.TCCGCGGCTTCCTCACCCCTGGC.CCC..GCCCCCCCCCCCCCggggcgg                                                  >  1:3863660/1‑98 (MQ=255)
                                                      gCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTg                                                <  1:6958066/101‑1 (MQ=255)
                                                       cAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                               >  1:2363854/1‑101 (MQ=255)
                                                         tctgcgcggTCGCGCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                              <  2:3009688/94‑1 (MQ=2)
                                                         gTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  2:125178/1‑101 (MQ=255)
                                                         gTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GA.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  2:3197487/1‑101 (MQ=255)
                                                          tGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:3595486/101‑1 (MQ=255)
                                                          tGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:5159378/101‑1 (MQ=255)
                                                          tGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  2:7956998/1‑101 (MQ=255)
                                                          gggACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:1049657/100‑1 (MQ=255)
                                                          ctgcgcggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:1685241/95‑1 (MQ=2)
                                                           tgcgcggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  2:4534696/6‑101 (MQ=2)
                                                           tgcgcggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:3481374/96‑1 (MQ=2)
                                                             cttGGTCGCTACCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:8099240/99‑1 (MQ=255)
                                                              gcggTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         >  2:356669/3‑101 (MQ=2)
                                                              cTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  2:6081721/1‑101 (MQ=255)
                                                              cTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  2:4796459/101‑1 (MQ=255)
                                                               tGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaag                                       >  2:8098891/1‑100 (MQ=255)
                                                               tGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  1:877667/101‑1 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                      >  1:978268/1‑100 (MQ=2)
                                                                 gTCGCGCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                     <  2:3774205/101‑1 (MQ=255)
                                                                  tCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                     >  2:8296902/1‑99 (MQ=2)
                                                                   cGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                    <  1:4279260/101‑4 (MQ=2)
                                                                   cGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                   >  2:8741892/1‑101 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                   >  1:1896110/1‑101 (MQ=255)
                                                                    gCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                   >  1:1417545/1‑97 (MQ=2)
                                                                    gCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGCGGGCGTGGATCGAAACAGgcg                                  >  2:4948237/1‑101 (MQ=255)
                                                                     cTCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                  <  1:61432/101‑6 (MQ=2)
                                                                     cTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                 <  2:5232737/101‑1 (MQ=255)
                                                                      tCCCCCCGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                 >  1:9026087/1‑95 (MQ=2)
                                                                        cccccGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                               <  1:6245237/101‑9 (MQ=2)
                                                                        cccccGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                               >  1:1480986/1‑93 (MQ=2)
                                                                        cccccGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATc                              <  1:4404774/101‑1 (MQ=255)
                                                                         ccccGCGGGGGC.GTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATT.GCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                              <  2:5508728/101‑10 (MQ=2)
                                                                           ccGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGa                           <  2:5688203/101‑1 (MQ=255)
                                                                           ccGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGa                           >  2:6195168/1‑101 (MQ=255)
                                                                              cgGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                        >  1:8417623/1‑101 (MQ=255)
                                                                              cgGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                        >  1:7805909/1‑101 (MQ=255)
                                                                               gggggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..TTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCCCATCTGATGta                       >  1:1286911/1‑101 (MQ=255)
                                                                               gggggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                       >  2:5366149/1‑101 (MQ=255)
                                                                               gggggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                       >  2:1814854/1‑101 (MQ=255)
                                                                                ggggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                      <  1:5581431/101‑1 (MQ=255)
                                                                                 gggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTTCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                     >  1:8278466/1‑101 (MQ=255)
                                                                                  ggC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                    >  2:336789/1‑101 (MQ=255)
                                                                                   ggcgtgatccGAACCTCCCACGCGGTCCGCGCTTGGCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                    <  2:8519548/92‑1 (MQ=25)
                                                                                      gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                 <  2:5002235/101‑1 (MQ=255)
                                                                                       tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                >  1:3013337/1‑101 (MQ=255)
                                                                                        ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg               <  2:1443563/101‑1 (MQ=255)
                                                                                        ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg               >  1:7525048/1‑101 (MQ=255)
                                                                                          aTCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCNANNNAGGCGCATCTGCTGTATATGCCCGGt             >  1:1212440/1‑101 (MQ=255)
                                                                                           tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg            >  2:1871955/1‑101 (MQ=255)
                                                                                            cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga           >  1:5721678/1‑101 (MQ=255)
                                                                                             gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag          <  2:8990734/101‑1 (MQ=255)
                                                                                             gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag          >  2:6302377/1‑101 (MQ=255)
                                                                                             gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag          >  2:5150184/1‑101 (MQ=255)
                                                                                               accTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc        <  2:673548/99‑1 (MQ=255)
                                                                                                aCTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg       <  1:278130/101‑1 (MQ=255)
                                                                                                   cGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt    >  2:7143410/1‑101 (MQ=255)
                                                                                                   cGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt    <  1:3197586/101‑1 (MQ=255)
                                                                                                    gCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTg   <  1:6643031/101‑1 (MQ=255)
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TCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC.GTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCT.GC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGT  >  NC_014034/1395900‑1396100

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG