Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,025 C→T 15.9% intergenic (+837/‑94) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0250C→T15.9% 215.0 ‑0.9 ‑0.0496intergenic (+837/‑94)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (35/44):  ref base (209/207):  total (245/251)
Fisher's exact test strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.93e-01

Only 100 of 524 total aligned reads displayed.

CGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395925‑1396125
                                                                                                    |                                                                                                       
 gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                                                                                        <  2:8111220/101‑1 (MQ=255)
 gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                                                                                        >  2:421042/1‑101 (MQ=255)
  aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                                                                                                       <  2:9124223/101‑1 (MQ=255)
     cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cg                                                                                                   <  1:142827/101‑1 (MQ=255)
         gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTc                                                                                             <  1:8746220/101‑1 (MQ=255)
         gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTc                                                                                             <  2:644426/101‑1 (MQ=255)
          cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTcc                                                                                            >  2:317408/1‑100 (MQ=255)
          cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCg                                                                                            >  1:6480121/1‑101 (MQ=255)
          cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCg                                                                                            >  1:7296722/1‑101 (MQ=255)
              cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcc                                                                                        >  1:6763122/1‑101 (MQ=255)
                 ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGGTCCGCGCTTGCCTCACGCCCGC.CGC..GTCGGTccccc                                                                                     >  2:2934273/1‑101 (MQ=255)
                  gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcccccc                                                                                    <  2:4703270/101‑1 (MQ=255)
                   ccTACGGCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                   <  2:8801048/101‑1 (MQ=255)
                    cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgc                                                                                  <  1:855346/101‑1 (MQ=255)
                     tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                                 >  1:1251206/1‑101 (MQ=255)
                     tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                                 >  1:7614526/1‑101 (MQ=255)
                      aCGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCgg                                                                                >  1:6588421/1‑101 (MQ=255)
                          cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                            <  2:7811502/101‑1 (MQ=255)
                          cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                            <  1:4818303/101‑1 (MQ=255)
                            ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCgg                                                                          >  2:3649714/1‑100 (MQ=255)
                            ggCAGTGGACTGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                          <  1:3325985/101‑1 (MQ=255)
                             gCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGCCGTg                                                                         >  1:2899770/1‑101 (MQ=255)
                               aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                       <  1:4919727/101‑1 (MQ=255)
                               aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                       >  2:2132900/1‑101 (MQ=255)
                                gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      <  1:1771831/101‑1 (MQ=255)
                                gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGA.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      >  2:3197487/1‑101 (MQ=255)
                                gTGGACTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      <  1:9153011/101‑1 (MQ=255)
                                 tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     >  2:7956998/1‑101 (MQ=255)
                                 tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  2:3554479/101‑1 (MQ=255)
                                 tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  2:495620/101‑1 (MQ=255)
                                 gggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:1049657/100‑1 (MQ=255)
                                  tgcgcggTCCCTGCCCCCGCGGGGGCCTGGATCCAAACTCGGCCTCGATCCGATC.AGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  2:5301786/96‑1 (MQ=2)
                                  ggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    >  1:3857013/1‑101 (MQ=255)
                                  ggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGCGGATCg                                                                    >  2:6188057/1‑101 (MQ=255)
                                   gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  2:8898863/97‑1 (MQ=9)
                                   gACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                   <  2:3477225/101‑1 (MQ=255)
                                     cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  2:4889813/1‑101 (MQ=255)
                                     cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  2:6081721/1‑101 (MQ=255)
                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                >  2:7051158/1‑101 (MQ=2)
                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                <  2:4459135/101‑1 (MQ=2)
                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                >  2:3087942/1‑101 (MQ=2)
                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                               >  1:5990495/1‑101 (MQ=255)
                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                               <  1:121219/101‑1 (MQ=255)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                            <  2:4607404/101‑1 (MQ=255)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAAGgc                                                            >  2:5132238/1‑101 (MQ=255)
                                          cGCGCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCGCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                            <  1:8198508/101‑1 (MQ=255)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                                            >  2:3793456/1‑97 (MQ=2)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                           >  2:1869960/1‑101 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                                        >  2:525193/1‑93 (MQ=2)
                                               cccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                                        <  1:1655055/101‑9 (MQ=2)
                                               cccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATc                                                       <  2:5924046/101‑1 (MQ=255)
                                                 cccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacca                                                      <  2:1908269/101‑11 (MQ=2)
                                                 cccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTg                                                     <  1:8963838/101‑1 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGAt                                                   <  1:8371788/101‑1 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                                                 >  1:7805909/1‑101 (MQ=255)
                                                      gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGNNNNNGGATCGAAACAGGCGCATCTGATGta                                                >  1:5081803/1‑101 (MQ=255)
                                                       ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                               <  1:269557/101‑1 (MQ=255)
                                                         ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                             >  1:7470191/1‑101 (MQ=255)
                                                         ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                             <  1:5211697/101‑1 (MQ=255)
                                                         ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                             <  1:3571167/101‑1 (MQ=255)
                                                         ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                             >  2:336789/1‑101 (MQ=255)
                                                          gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                            <  2:7403376/101‑1 (MQ=255)
                                                           cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                           >  2:1802326/1‑101 (MQ=255)
                                                            gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                          <  1:6185745/101‑1 (MQ=255)
                                                            gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                          <  1:5832996/101‑1 (MQ=255)
                                                            gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                          <  2:4072565/101‑1 (MQ=255)
                                                             tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                         >  1:3013337/1‑101 (MQ=255)
                                                             tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                         <  2:7598465/101‑1 (MQ=255)
                                                                 tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                     <  2:1153832/101‑1 (MQ=255)
                                                                   gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                   >  2:6302377/1‑101 (MQ=255)
                                                                    aaaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgcgc                                  >  2:1371778/1‑98 (MQ=255)
                                                                    aaaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                  >  2:5396106/1‑101 (MQ=255)
                                                                     accTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                                 <  2:8603908/99‑1 (MQ=255)
                                                                     aaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                                 <  2:6789581/101‑1 (MQ=255)
                                                                      aCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                                <  1:6137702/101‑1 (MQ=255)
                                                                      aCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                                <  1:4409550/101‑1 (MQ=255)
                                                                       cTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACgg                               <  1:2102591/101‑1 (MQ=255)
                                                                         cccccTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                             <  1:1968351/99‑1 (MQ=255)
                                                                         cGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGAATATGCCCGGTGAGACGGCt                             >  1:5603814/1‑101 (MQ=255)
                                                                            ccTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                          <  1:3195914/101‑1 (MQ=255)
                                                                             cTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCg                         >  2:400373/1‑101 (MQ=255)
                                                                             cTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCg                         <  1:421050/101‑1 (MQ=255)
                                                                             cTCGAGCCGCGCTTGCCTCCCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCg                         <  2:7174791/101‑1 (MQ=255)
                                                                                gATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc                      >  1:6346827/1‑101 (MQ=255)
                                                                                gATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc                      >  1:6937142/1‑101 (MQ=255)
                                                                                gATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc                      >  2:5734570/1‑101 (MQ=255)
                                                                                 aTCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcc                     <  1:4312944/101‑1 (MQ=255)
                                                                                 aTCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcc                     >  1:7096394/1‑101 (MQ=255)
                                                                                   ccGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc                   <  2:1492697/101‑1 (MQ=255)
                                                                                     gcgcTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                 <  2:2465812/101‑1 (MQ=255)
                                                                                          tGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCggg            >  1:4034553/1‑101 (MQ=255)
                                                                                           cccTCACGCCTGC.GGC..GGCGCCCCCCCCGCGGGGGCGGGAATCGAACCAGGCGCATTTGATTTATATCCCCGGTGAGACGGCCGTCGCTCCCCCCGCgggg           <  2:8407956/100‑1 (MQ=255)
                                                                                             cTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc         >  1:6665342/1‑101 (MQ=255)
                                                                                             cTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc         <  2:5560110/101‑1 (MQ=255)
                                                                                               cACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCgg       >  2:282704/1‑100 (MQ=255)
                                                                                               cACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGt       >  1:2443479/1‑101 (MQ=255)
                                                                                                aCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGCTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTg      >  1:8026446/1‑101 (MQ=255)
                                                                                                  gCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGa    <  2:6653044/101‑1 (MQ=255)
                                                                                                   ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt   <  2:1040714/101‑1 (MQ=255)
                                                                                                   ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt   >  1:1145830/1‑101 (MQ=255)
                                                                                                    |                                                                                                       
CGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395925‑1396125

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG