Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,153 C→T 4.2% A12V (GCG→GTA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1530C→T 4.2% 27.2 0.0 ‑0.0393A12V (GCG→GTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/9):  ref base (179/197):  total (187/206)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.30e-01

Only 100 of 482 total aligned reads displayed.

TGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGG  >  NC_014034/1396053‑1396252
                                                                                                      |                                                                                                   
 ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgcg                                                                                                    >  2:1853852/1‑101 (MQ=255)
 ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcccg                                                                                                    >  2:621798/1‑101 (MQ=255)
     cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                >  1:4657888/1‑101 (MQ=255)
     cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                >  1:4511362/1‑101 (MQ=255)
     cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                <  1:2820664/101‑1 (MQ=255)
      gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                               >  1:1514114/1‑101 (MQ=255)
      gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                               <  2:7524724/101‑1 (MQ=255)
      gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                               <  2:4807307/101‑1 (MQ=255)
      gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                               >  1:7555895/1‑101 (MQ=255)
       aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGGgccgccc                                                                                              >  2:4044254/1‑100 (MQ=255)
       aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgccc                                                                                              >  2:6958737/1‑100 (MQ=255)
       aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgccc                                                                                              >  2:4611898/1‑100 (MQ=255)
       aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgccc                                                                                              >  1:8310594/1‑100 (MQ=255)
       aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgccc                                                                                              >  1:7936461/1‑100 (MQ=255)
              cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACCCTGCg                                                                                       >  2:8172816/1‑101 (MQ=255)
                  tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGc                                                                                   >  1:8095877/1‑101 (MQ=255)
                  tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGc                                                                                   >  2:4486296/1‑101 (MQ=255)
                    tGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTc                                                                                 <  1:5954975/101‑1 (MQ=255)
                      aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGGCGCCACGCTGGGGCGCTccc                                                                               >  2:4725764/1‑101 (MQ=255)
                       tGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTcccc                                                                              >  2:5098327/1‑101 (MQ=255)
                        gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                                             >  2:3117712/1‑101 (MQ=255)
                         tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTcccccc                                                                            >  2:7684470/1‑101 (MQ=255)
                         tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                            <  1:7143714/101‑1 (MQ=255)
                          gtatGCCCGTGAAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCTTC.ATCTGT.CAGGCTCGCGCCGCCACGCTGGGTCGCTCCCCCcg                                                                           <  2:1228824/100‑1 (MQ=37)
                          atatGCCCGGTGAGCCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcg                                                                           <  2:528259/101‑1 (MQ=255)
                          atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCCccccccc                                                                           >  2:3681759/1‑100 (MQ=255)
                          atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccccc                                                                           >  1:2318411/1‑100 (MQ=255)
                           tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CATGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcgc                                                                          <  1:6442044/101‑1 (MQ=255)
                            atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCCCCCCCcgcg                                                                         >  1:3430977/1‑101 (MQ=255)
                             tGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgg                                                                        <  2:8059091/101‑1 (MQ=255)
                               cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgggg                                                                      <  1:1099057/101‑1 (MQ=255)
                               cccGGTGAGACGGCTGTCCCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgggg                                                                      <  2:8674216/101‑1 (MQ=255)
                                 cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGc                                                                    <  2:7779460/101‑1 (MQ=255)
                                   gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGt                                                                  <  2:8842963/101‑1 (MQ=255)
                                   gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCCCCCCCCCCGGGGGCgg                                                                  >  1:3181683/1‑100 (MQ=255)
                                    tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                 <  2:6075539/91‑1 (MQ=9)
                                    tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCggg                                                                 >  1:1890643/1‑99 (MQ=255)
                                    tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTg                                                                 <  1:125178/101‑1 (MQ=255)
                                    ggagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTg                                                                 <  1:2934371/100‑1 (MQ=255)
                                     gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                <  1:2622984/92‑1 (MQ=2)
                                       gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              >  1:6314088/1‑101 (MQ=255)
                                        aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:6283731/101‑1 (MQ=255)
                                        aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:4185381/101‑1 (MQ=255)
                                         cGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  2:1043327/101‑1 (MQ=255)
                                           gCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  1:3292616/101‑1 (MQ=255)
                                            cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         <  2:6350360/101‑1 (MQ=255)
                                            cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         <  2:3937048/101‑1 (MQ=255)
                                            cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  2:3541357/1‑101 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTNCCCCCGCGGGGGCGTGGATCGAAAcc                                                       >  1:8265454/1‑101 (MQ=255)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATGGAAAccc                                                      >  2:1487161/1‑101 (MQ=255)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                      <  2:2651606/101‑1 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACCCg                                                     >  2:7288275/1‑101 (MQ=255)
                                                cGCGCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.TTCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                                     <  1:6558270/101‑1 (MQ=255)
                                                   gccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCg                                                  <  1:4742575/100‑1 (MQ=255)
                                                      ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCCGGCGaaa                                               >  1:5688316/1‑101 (MQ=255)
                                                           cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                          >  2:8269533/1‑101 (MQ=255)
                                                             ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                                        <  1:4053633/101‑1 (MQ=255)
                                                             ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                                        <  1:1802379/101‑1 (MQ=255)
                                                             ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                                        <  1:4898839/101‑1 (MQ=255)
                                                              gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCa                                       <  1:77103/101‑1 (MQ=255)
                                                              gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGCGGGCGTGGATCGAAACCCGGCGAAAAACGGCCa                                       <  1:6461569/101‑1 (MQ=255)
                                                               ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                      >  2:6284372/1‑101 (MQ=255)
                                                               ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                      >  1:8497010/1‑101 (MQ=255)
                                                               ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                      <  1:9113402/101‑1 (MQ=255)
                                                                gCGGGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                                     <  2:3329409/101‑1 (MQ=255)
                                                                 cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGc                                    >  1:3075988/1‑101 (MQ=255)
                                                                  gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCCGGCGCAAAACGGCCATGcc                                   >  1:2472511/1‑101 (MQ=255)
                                                                   tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                                  <  1:7832102/101‑1 (MQ=255)
                                                                   tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGAAAAACGGCCATGCCt                                  >  2:6535711/1‑101 (MQ=255)
                                                                    ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                                 >  2:5536787/1‑101 (MQ=255)
                                                                       tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgg                              <  2:2472438/101‑1 (MQ=255)
                                                                        cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCggg                             <  2:4930661/101‑1 (MQ=255)
                                                                         gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgt                            <  2:1348209/101‑1 (MQ=255)
                                                                           aaCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGCAAAACGGCCATGCCCCCGGgtgc                          >  1:8672730/1‑100 (MQ=255)
                                                                            aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc                         >  2:2213056/1‑101 (MQ=255)
                                                                            aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc                         <  1:6959022/101‑1 (MQ=255)
                                                                              ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                       <  1:3725093/101‑1 (MQ=255)
                                                                               tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                      >  1:3410288/1‑101 (MQ=255)
                                                                               tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTgg                      >  2:5891010/1‑101 (MQ=255)
                                                                               tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTgg                      >  2:1755341/1‑101 (MQ=255)
                                                                                ttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc                     <  2:2345013/101‑1 (MQ=255)
                                                                                   gTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGTGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGt                  <  1:614423/101‑1 (MQ=255)
                                                                                   gTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGt                  <  2:8479589/101‑1 (MQ=255)
                                                                                    tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTggc                 >  1:4278237/1‑99 (MQ=255)
                                                                                    tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                 >  2:9093514/1‑101 (MQ=255)
                                                                                     c.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                >  1:8985048/1‑101 (MQ=255)
                                                                                         cTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTc             >  1:6068341/1‑101 (MQ=255)
                                                                                          tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc            >  2:5706326/1‑101 (MQ=255)
                                                                                          tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc            <  2:3249375/101‑1 (MQ=255)
                                                                                           gg.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccc           >  1:205558/1‑101 (MQ=255)
                                                                                            g.CAGGCTCGCGCCGCCAGGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccc          <  2:1973420/101‑1 (MQ=255)
                                                                                              cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc         >  2:7835780/1‑101 (MQ=255)
                                                                                              cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc         >  1:5077135/1‑101 (MQ=255)
                                                                                               aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCTCTcccccc        >  1:6711101/1‑101 (MQ=255)
                                                                                               aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc        >  1:5286025/1‑101 (MQ=255)
                                                                                                ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcg       >  1:2146152/1‑101 (MQ=255)
                                                                                                 gCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcgc      >  1:3608080/1‑101 (MQ=255)
                                                                                                   tCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCgg    >  1:730006/1‑101 (MQ=255)
                                                                                                    cgcgCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggg   >  2:288661/1‑101 (MQ=255)
                                                                                                    cgcgCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggg   >  1:7469788/1‑101 (MQ=255)
                                                                                                      |                                                                                                   
TGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGG  >  NC_014034/1396053‑1396252

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG