Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,558 A→G 17.0% Q147R (CAG→CGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5580A→G17.0% 193.0 ‑1.7 ‑0.0477Q147R (CAG→CGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (47/34):  ref base (196/200):  total (243/234)
Fisher's exact test strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.45e-01

Only 100 of 506 total aligned reads displayed.

GTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396458‑1396658
                                                                                                      |                                                                                                    
gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCa                                                                                                      <  2:5563007/101‑1 (MQ=255)
  ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgc                                                                                                    >  1:6307151/1‑101 (MQ=255)
  ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgc                                                                                                    >  1:5027272/1‑101 (MQ=255)
  ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgc                                                                                                    >  1:3097025/1‑101 (MQ=255)
   gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgcc                                                                                                   >  2:3803535/1‑101 (MQ=255)
    aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgccg                                                                                                  >  2:2623600/1‑101 (MQ=255)
    aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgccg                                                                                                  <  1:3868307/101‑1 (MQ=255)
      cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgccgcc                                                                                                >  1:4279617/1‑101 (MQ=255)
      cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgccgcc                                                                                                <  1:4197814/101‑1 (MQ=255)
       gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGccgccc                                                                                               >  2:4786663/1‑101 (MQ=255)
         aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGccgcccgc                                                                                             <  1:1072603/101‑1 (MQ=255)
         aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGccgcccgc                                                                                             >  1:4089767/1‑101 (MQ=255)
           cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCgcgc                                                                                           >  2:1859483/1‑101 (MQ=255)
            ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGcc                                                                                          >  1:2517674/1‑101 (MQ=255)
            ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGcc                                                                                          >  1:6756228/1‑101 (MQ=255)
               ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAg                                                                                       <  2:7087912/101‑1 (MQ=255)
                 tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTc                                                                                     <  1:2243949/101‑1 (MQ=255)
                  gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTca                                                                                    >  2:2243882/1‑100 (MQ=255)
                  gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCCCCCGCGCCAAGTcc                                                                                    >  1:8615846/1‑100 (MQ=255)
                    aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                                                                                  >  1:176944/1‑101 (MQ=255)
                     aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                                 <  2:5159119/101‑1 (MQ=255)
                     aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                                 <  1:6429326/101‑1 (MQ=255)
                      ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcc                                                                                >  2:6224847/1‑101 (MQ=255)
                          gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                            <  2:2180935/101‑1 (MQ=255)
                           cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg                                                                           >  1:5430986/1‑101 (MQ=255)
                           cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg                                                                           <  1:6592832/101‑1 (MQ=255)
                                aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                      >  2:3488726/1‑101 (MQ=255)
                                       ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                <  1:7297312/91‑1 (MQ=2)
                                        tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                               <  2:1699555/92‑1 (MQ=2)
                                         gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                              <  2:836153/93‑1 (MQ=2)
                                         gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGGGGAt                                                              >  2:4879543/9‑101 (MQ=2)
                                         gcgTGCACGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:4219215/101‑1 (MQ=255)
                                         gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:2446602/101‑1 (MQ=255)
                                         gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:2016439/101‑1 (MQ=255)
                                         gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:7246719/101‑1 (MQ=255)
                                          cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            >  1:521068/1‑101 (MQ=255)
                                           gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                            <  1:4812546/95‑1 (MQ=2)
                                           gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGGGGGGATCGa                                                           >  1:3576672/1‑101 (MQ=255)
                                            acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                           <  1:769286/96‑1 (MQ=2)
                                            acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                           <  2:1406506/96‑1 (MQ=2)
                                            aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  2:4480472/101‑1 (MQ=255)
                                            aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  2:3613057/101‑1 (MQ=255)
                                            aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  2:2891993/101‑1 (MQ=255)
                                             cggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaa                                                          >  1:4447933/5‑101 (MQ=2)
                                             cggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaa                                                          <  1:1835376/97‑1 (MQ=2)
                                              ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                         <  1:8306271/98‑1 (MQ=2)
                                               gagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAc                                                        >  2:5476545/3‑101 (MQ=2)
                                               ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                       <  2:6668624/100‑1 (MQ=255)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.A.GTGGGACTGGCCAGCCGCCCGAGCCAAGCCGCTCCCCCACGGGGGGCGGGGTTCGAGACGac                                                     >  2:684679/1‑100 (MQ=25)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActg                                                      <  1:4913027/101‑3 (MQ=2)
                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                                     <  2:2377083/101‑4 (MQ=2)
                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                   >  2:5865567/1‑101 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                  <  1:325900/101‑1 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                   <  2:1165461/101‑6 (MQ=2)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGGCGCTCCCCCCGCCGGGGCGTGGATCGAAACCGTTtcg                                                 >  2:5055103/1‑101 (MQ=25)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                >  1:2130804/1‑101 (MQ=255)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                >  1:5501193/1‑101 (MQ=255)
                                                       ccccccGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                               >  1:5923005/1‑101 (MQ=255)
                                                       ccccccGCGGGGGCGTGGATCGAAACAGCTTTCC.ATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                >  1:8014537/1‑93 (MQ=2)
                                                       ccccccGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCATCCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                >  1:6670752/1‑93 (MQ=2)
                                                       ccccccGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                <  1:8502639/101‑9 (MQ=2)
                                                        cccccGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                               >  2:789928/1‑92 (MQ=2)
                                                        cccccGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                               <  1:7772580/101‑10 (MQ=2)
                                                              cgGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                                        <  1:5115135/101‑1 (MQ=255)
                                                               gggggCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTTTCGTCGCTTaccg                                       >  2:8665506/1‑99 (MQ=255)
                                                               gggggCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                       >  1:9103560/1‑101 (MQ=255)
                                                                ggggCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                      >  2:8058480/1‑101 (MQ=255)
                                                                    cGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                  <  1:2821326/101‑1 (MQ=255)
                                                                    cGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                  <  1:6051380/101‑1 (MQ=255)
                                                                    cGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                  >  1:4070500/1‑101 (MQ=255)
                                                                     gTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                 <  2:7250072/101‑1 (MQ=255)
                                                                     gTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                 >  1:1645361/1‑101 (MQ=255)
                                                                     gTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                 <  1:9041481/101‑1 (MQ=255)
                                                                      tGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                >  1:8919129/1‑101 (MQ=255)
                                                                      tGGATCGAAACTGCTTT.C.GTAGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                <  1:7135338/101‑1 (MQ=255)
                                                                       ggATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAACGATATAGTCGCTTACGACGCCTATc                               >  2:2736328/1‑101 (MQ=255)
                                                                       ggATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                               <  1:3331626/101‑1 (MQ=255)
                                                                       ggATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                               <  1:8146146/101‑1 (MQ=255)
                                                                       ggATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                               >  2:9185326/1‑101 (MQ=255)
                                                                           cGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                           >  1:4706084/1‑101 (MQ=255)
                                                                            gAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                          >  2:7501294/1‑101 (MQ=255)
                                                                            gAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                          <  2:176941/101‑1 (MQ=255)
                                                                             aaaCTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                         >  2:5651656/1‑101 (MQ=255)
                                                                              aaCTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc                        <  1:1982318/101‑1 (MQ=255)
                                                                                  gCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                    >  2:7186950/1‑101 (MQ=255)
                                                                                   cTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc                   >  2:5270094/1‑101 (MQ=255)
                                                                                   cTTT.C.GGGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTc                   <  2:3223857/101‑1 (MQ=255)
                                                                                    ttt.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                  >  2:4397492/1‑101 (MQ=255)
                                                                                        c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTTTCGTCGCTTACGACGCCTATCTGATTTCGGGGCGCTc               >  1:1368578/1‑101 (MQ=255)
                                                                                        c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc               >  2:8854292/1‑101 (MQ=255)
                                                                                        c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc               >  2:5406761/1‑101 (MQ=255)
                                                                                          gTGGGCCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc              <  2:637531/101‑1 (MQ=255)
                                                                                          gTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc              >  1:11379/1‑101 (MQ=255)
                                                                                             ggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc           <  1:7856676/101‑1 (MQ=255)
                                                                                                cTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc        >  1:3533386/1‑101 (MQ=255)
                                                                                                 tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccccaga       >  2:4071241/1‑98 (MQ=255)
                                                                                                 tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg       >  1:7568414/1‑101 (MQ=255)
                                                                                                    ccAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg    >  1:5653945/1‑101 (MQ=255)
                                                                                                     cAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggggg   <  1:699903/101‑1 (MQ=255)
                                                                                                      aGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc  >  1:2381020/1‑101 (MQ=255)
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GTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396458‑1396658

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG