Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,561 C→G 18.1% P148R (CCG→CGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5610C→G18.1% 200.3 ‑2.0 ‑0.0446P148R (CCG→CGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (47/34):  ref base (177/188):  total (224/222)
Fisher's exact test strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.65e-01

Only 100 of 493 total aligned reads displayed.

GATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396461‑1396661
                                                                                                      |                                                                                                    
gATCGAAACGGACCTGAAATTCCGCGGGAANCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgcc                                                                                                      <  1:4172817/101‑1 (MQ=255)
gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgcc                                                                                                      >  2:6075382/1‑101 (MQ=255)
 aTCGAAACGGACCTGAAATTCCGCGGGAAACGGGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgccg                                                                                                     <  1:4675933/101‑1 (MQ=255)
 aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgccg                                                                                                     >  2:2623600/1‑101 (MQ=255)
  acGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAgccgc                                                                                                    <  1:4985665/100‑1 (MQ=255)
    gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGccgccc                                                                                                  >  2:7242782/1‑101 (MQ=255)
    gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGccgccc                                                                                                  <  1:7296727/101‑1 (MQ=255)
      aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGccgcccgc                                                                                                <  1:1072603/101‑1 (MQ=255)
        cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCgcgc                                                                                              >  2:1859483/1‑101 (MQ=255)
        cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCCCCCgcgc                                                                                              >  2:4131479/1‑101 (MQ=255)
          gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCa                                                                                            >  1:1125857/1‑101 (MQ=255)
           aCCAGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGAATGGCCAGCCGCCCGCGCCaa                                                                                           >  1:7209757/1‑101 (MQ=255)
            ccTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAg                                                                                          <  2:7087912/101‑1 (MQ=255)
               gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTca                                                                                       >  2:2243882/1‑100 (MQ=255)
               gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCg                                                                                       >  1:2138127/1‑101 (MQ=255)
               gAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCCCCCGCGCCAAGTcc                                                                                       >  1:8615846/1‑100 (MQ=255)
                aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                                                                                      >  1:7078034/1‑101 (MQ=255)
                 aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                                                                                     >  1:176944/1‑101 (MQ=255)
                  aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                                    <  2:6306911/101‑1 (MQ=255)
                  aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                                    >  1:5092996/1‑101 (MQ=255)
                     ccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccc                                                                                 <  1:2363023/101‑1 (MQ=255)
                      cgctgtaaccgcgGCGGGGAGCGCGTCTCGCCCCCCGCGGGCGCGTGGATCGAAACTGCTTT.C.GTGGGACCGGCCAGCCGCCCGCGCCAAGTCGCTccccc                                                                                <  2:4676613/92‑1 (MQ=37)
                       gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                               <  2:286709/101‑1 (MQ=255)
                       gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                               <  2:2180935/101‑1 (MQ=255)
                       gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                               <  1:4803455/101‑1 (MQ=255)
                          ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg                                                                            >  1:5354201/1‑101 (MQ=255)
                               cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                                       <  1:8479119/101‑1 (MQ=255)
                                    cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTTGCCAGCCGCCGCCGCCAAGTCGCTCCCCCCGCGGGGGCGCGGa                                                                  >  2:8384907/1‑101 (MQ=37)
                                    cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  2:3463871/1‑101 (MQ=255)
                                     tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                  <  2:1699555/92‑1 (MQ=2)
                                      gcgTGCACGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:4219215/101‑1 (MQ=255)
                                      gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:2016439/101‑1 (MQ=255)
                                      gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:2446602/101‑1 (MQ=255)
                                       cgAGCACGTCGCTCCCCCCGGGGGGGCGTGTATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  1:7664366/101‑1 (MQ=255)
                                       cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTAGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  1:5890450/1‑101 (MQ=255)
                                        gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAtag                                                               >  2:3601700/7‑99 (MQ=2)
                                        gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              <  1:547543/101‑1 (MQ=255)
                                        gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  1:2892091/1‑101 (MQ=255)
                                         acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                              <  2:1406506/96‑1 (MQ=2)
                                          cggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaa                                                             <  1:1835376/97‑1 (MQ=2)
                                           ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                            >  1:666391/4‑101 (MQ=2)
                                           ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                            <  1:5187410/98‑1 (MQ=2)
                                           ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                            >  2:4458350/4‑101 (MQ=2)
                                           ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                            <  1:8306271/98‑1 (MQ=2)
                                            gagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAc                                                           <  2:7175971/99‑1 (MQ=2)
                                            gagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGCGGTGGACCGAAAc                                                           >  1:4234097/3‑101 (MQ=2)
                                            ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                          <  2:6668624/100‑1 (MQ=255)
                                             cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGa                                                         >  2:8667423/1‑101 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                        >  1:2074101/1‑101 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.A.GTGGGACTGGCCAGCCGCCCGAGCCAAGCCGCTCCCCCACGGGGGGCGGGGTTCGAGACGac                                                        >  2:684679/1‑100 (MQ=25)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActg                                                         >  2:3154658/1‑99 (MQ=2)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAtt                                                       <  1:641960/101‑1 (MQ=255)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                                        <  2:2377083/101‑4 (MQ=2)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgg                                                        >  2:5367929/1‑98 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                     <  1:325900/101‑1 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                     <  1:671691/101‑1 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                      <  2:1165461/101‑6 (MQ=2)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                                    >  2:379858/1‑101 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                   >  2:9188161/1‑101 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                                    >  2:4839835/1‑94 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                  <  1:3998487/101‑1 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACAGCTTTCC.ATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                   >  1:8014537/1‑93 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                   >  2:81110/1‑93 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                   >  1:3711349/1‑93 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                  >  2:789928/1‑92 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACAGCTTT.CAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                  <  1:7772580/101‑10 (MQ=2)
                                                         cgcgGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                             <  2:5236297/101‑1 (MQ=255)
                                                            gggggCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                          >  1:9103560/1‑101 (MQ=255)
                                                                  gTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                    >  1:3566926/1‑101 (MQ=255)
                                                                  gTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                    <  1:9041481/101‑1 (MQ=255)
                                                                  gTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                    >  1:1645361/1‑101 (MQ=255)
                                                                   tGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                   >  2:4996507/1‑101 (MQ=255)
                                                                   tGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                   >  1:8919129/1‑101 (MQ=255)
                                                                    ggATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAACGATATAGTCGCTTACGACGCCTATc                                  >  2:2736328/1‑101 (MQ=255)
                                                                    ggATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                  <  1:8146146/101‑1 (MQ=255)
                                                                    ggATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                  <  1:3331626/101‑1 (MQ=255)
                                                                     gATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATcc                                 >  2:5457019/1‑100 (MQ=255)
                                                                      aTCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                <  1:2515284/101‑1 (MQ=255)
                                                                      aTCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                                <  1:4078494/101‑1 (MQ=255)
                                                                       tCGAAACTGCTTC.C.GTGGGACTGGCCAGGCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGGCGCTTACGACGCCTATCTGa                               >  1:6509399/1‑101 (MQ=255)
                                                                        cGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                              <  2:5548506/101‑1 (MQ=255)
                                                                         gAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                             <  2:176941/101‑1 (MQ=255)
                                                                         gAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                             >  2:5589661/1‑101 (MQ=255)
                                                                         gAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                             >  2:7501294/1‑101 (MQ=255)
                                                                          aaaCTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                            <  1:2804915/101‑1 (MQ=255)
                                                                          aaaCTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                            >  1:4868024/1‑101 (MQ=255)
                                                                             cTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg                         >  2:5134484/1‑101 (MQ=255)
                                                                               gCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                       >  1:5625294/1‑101 (MQ=255)
                                                                                 ttt.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                     >  2:4397492/1‑101 (MQ=255)
                                                                                     c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTTTCGTCGCTTACGACGCCTATCTGATTTCGGGGCGCTc                  >  1:1368578/1‑101 (MQ=255)
                                                                                        tGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc                >  2:5709272/1‑101 (MQ=255)
                                                                                        tGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc                >  2:9038527/1‑101 (MQ=255)
                                                                                          ggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc              <  1:7856676/101‑1 (MQ=255)
                                                                                          ggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc              <  1:7395861/101‑1 (MQ=255)
                                                                                            ccTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg            <  1:6025353/100‑1 (MQ=255)
                                                                                             cTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc           >  1:3533386/1‑101 (MQ=255)
                                                                                                  cAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggggg      >  1:4389684/1‑101 (MQ=255)
                                                                                                   cgccgccCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc     <  2:5882984/100‑1 (MQ=255)
                                                                                                   aGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc     <  1:6252197/101‑1 (MQ=255)
                                                                                                    gccgccCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCg    <  1:6804108/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
GATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTT.C.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396461‑1396661

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG