Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,680 A→C 1.0% M188L (ATA→CTA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,6800A→C 1.0% 2.4 0.0 ‑0.1497M188L (ATA→CTA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (247/245):  total (250/247)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.90e-01

Only 100 of 520 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCC  >  NC_014034/1396581‑1396780
                                                                                                   |                                                                                                    
ccccGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGAt                                                                                                     <  1:2018129/101‑1 (MQ=255)
 cccGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATa                                                                                                    <  2:3576564/101‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATaa                                                                                                   <  1:9025658/101‑1 (MQ=255)
   cgcgGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAAc                                                                                                  <  2:9064179/101‑1 (MQ=255)
        gggCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGct                                                                                             >  2:5587205/1‑101 (MQ=255)
         ggCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGctc                                                                                            >  2:7388833/1‑101 (MQ=255)
          gCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGctct                                                                                           >  1:8319713/1‑101 (MQ=255)
           cGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCtt                                                                                          <  2:6410038/101‑1 (MQ=255)
           cGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCtt                                                                                          <  2:4279503/101‑1 (MQ=255)
            gTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTg                                                                                         <  1:4472399/101‑1 (MQ=255)
            gTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTg                                                                                         >  2:2648172/1‑101 (MQ=255)
            gTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCTAACTCGCTCTTg                                                                                         >  2:8525587/1‑101 (MQ=255)
            gTGGAGTGAAACGATTTCGTCGCTTACGACACCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGTGATAACTCGCTCTTg                                                                                         <  1:638837/101‑1 (MQ=38)
              ggATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCg                                                                                       >  2:514972/1‑101 (MQ=255)
              ggATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCg                                                                                       >  2:8237210/1‑101 (MQ=255)
               gATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGGTGAGTCGCTCTTGCgc                                                                                      >  2:3887013/1‑100 (MQ=255)
               gATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGa                                                                                      <  1:5584179/101‑1 (MQ=255)
                 tCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATc                                                                                    <  2:2736641/101‑1 (MQ=255)
                  cGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATcc                                                                                   <  2:3704697/101‑1 (MQ=255)
                  cGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATcc                                                                                   <  1:1390043/101‑1 (MQ=255)
                    aaaCGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGt                                                                                 >  2:7613773/1‑101 (MQ=255)
                    aaaCGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGt                                                                                 <  1:5797790/101‑1 (MQ=255)
                       cGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCAt                                                                              <  1:3994471/101‑1 (MQ=255)
                        gATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCAtc                                                                             <  2:7742987/101‑1 (MQ=255)
                         aTTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCAtcg                                                                            <  1:2800110/101‑1 (MQ=255)
                         aTTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCAtcg                                                                            >  2:8306396/1‑101 (MQ=255)
                         aTTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCAtcg                                                                            <  1:1604461/101‑1 (MQ=255)
                          tttCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCAtcgt                                                                           >  2:7832326/1‑101 (MQ=255)
                           ttCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCAtcgtc                                                                          <  2:2074039/101‑1 (MQ=255)
                             cgtcgCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGc                                                                        <  1:3029768/101‑1 (MQ=255)
                             cgtcgCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGc                                                                        >  2:6671073/1‑101 (MQ=255)
                             cgtcgCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGc                                                                        >  2:477406/1‑101 (MQ=255)
                                 gCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTccc                                                                    <  2:6890265/101‑1 (MQ=255)
                                 gCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTccc                                                                    >  1:3827347/1‑101 (MQ=255)
                                  cTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTcccc                                                                   <  2:1645314/101‑1 (MQ=255)
                                    tACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATGACCCACTCTTCCGCTCCGCCATCGTCGCTcccccc                                                                 >  2:240364/1‑101 (MQ=37)
                                    tACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTcccccc                                                                 >  2:4170602/1‑101 (MQ=255)
                                       gacgCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCcgcg                                                              >  1:3686242/1‑101 (MQ=255)
                                         cgCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCggg                                                            >  2:3993560/1‑101 (MQ=255)
                                          gCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCgggg                                                           <  2:789818/101‑1 (MQ=255)
                                          gCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCgggg                                                           >  1:7941067/1‑101 (MQ=255)
                                          gCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCgggg                                                           >  2:3025572/1‑101 (MQ=255)
                                            cTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGc                                                         <  1:1713824/101‑1 (MQ=255)
                                            cTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGc                                                         >  1:8574640/1‑101 (MQ=255)
                                              aTCTGATTTCGGGTTGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGTGCgg                                                       >  1:7042746/1‑100 (MQ=255)
                                              aTCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGt                                                       >  1:4859035/1‑101 (MQ=255)
                                               tCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTg                                                      >  1:6639307/1‑101 (MQ=255)
                                                  gATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  1:1807168/1‑101 (MQ=255)
                                                  gATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  1:5042403/1‑101 (MQ=255)
                                                    tttCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  1:2457094/1‑101 (MQ=255)
                                                    tttCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  2:5500986/101‑1 (MQ=255)
                                                     ttCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                >  1:6234156/1‑101 (MQ=255)
                                                     ttCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  2:808990/101‑1 (MQ=255)
                                                     ttCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  1:2720383/101‑1 (MQ=255)
                                                      tCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               <  1:2576831/101‑1 (MQ=255)
                                                       cGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              <  2:1072852/101‑1 (MQ=255)
                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                            >  1:8750685/1‑101 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGGGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGt                                        <  1:8226506/101‑1 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGt                                        <  2:5922770/101‑1 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGt                                        >  1:602200/1‑101 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGt                                        >  1:7723260/1‑101 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTccc                                     >  2:8886205/1‑101 (MQ=255)
                                                                 cccccGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCt                                    >  2:6062935/1‑101 (MQ=255)
                                                                 cccccGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCt                                    <  2:7158705/101‑1 (MQ=255)
                                                                  ccccGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTc                                   <  2:2280767/101‑1 (MQ=255)
                                                                  ccccGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTc                                   <  1:6758763/101‑1 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCat                                 <  1:3252870/101‑1 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCat                                 >  1:4210150/1‑101 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCat                                 <  1:4648841/101‑1 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCatat                               <  2:5260430/101‑1 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCatat                               <  1:533522/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATAtg                              <  1:7389157/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATAtg                              <  1:5651863/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATAtg                              <  2:1522758/101‑1 (MQ=255)
                                                                          gggCGTGGATCGCAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATAtgctg                           >  1:8188235/1‑101 (MQ=255)
                                                                           ggCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATAtgctgc                          <  1:7179443/101‑1 (MQ=255)
                                                                           ggCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATAtgctgc                          >  2:5667851/1‑101 (MQ=255)
                                                                            gCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGcc                         <  2:4617411/101‑1 (MQ=255)
                                                                            gCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGcc                         <  1:7997999/101‑1 (MQ=255)
                                                                             cGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCa                        >  2:7521449/1‑101 (MQ=255)
                                                                              ggggATCGACACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCAc                       <  2:1368546/99‑1 (MQ=255)
                                                                              gTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCAc                       >  2:6173206/1‑101 (MQ=255)
                                                                               tGGGTCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACg                      <  1:1923155/101‑1 (MQ=255)
                                                                               tGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACg                      >  1:3482051/1‑101 (MQ=255)
                                                                                ggATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGa                     >  1:75345/1‑101 (MQ=255)
                                                                                 gATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGAt                    >  2:1789722/1‑101 (MQ=255)
                                                                                 gATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGAt                    <  2:1741526/101‑1 (MQ=255)
                                                                                  aTCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATg                   <  2:6318913/101‑1 (MQ=255)
                                                                                    cGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGcc                 >  1:101209/1‑101 (MQ=255)
                                                                                      aaaCCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCt               <  2:7974719/101‑1 (MQ=255)
                                                                                       aaCCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCtt              >  1:9147802/1‑101 (MQ=255)
                                                                                         ccATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAg           >  2:3185411/1‑101 (MQ=255)
                                                                                          cATGCGGCGATAACTCGCTCATGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAg           <  1:7218707/101‑1 (MQ=255)
                                                                                           aTGCGGCTATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGt          <  2:3551102/101‑1 (MQ=255)
                                                                                           aTGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGt          >  2:8439850/1‑101 (MQ=255)
                                                                                            tGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTc         <  2:1212608/101‑1 (MQ=255)
                                                                                              cggcgATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGc       <  1:2273140/101‑1 (MQ=255)
                                                                                              cggcgATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGc       >  1:1255375/1‑101 (MQ=255)
                                                                                                 cgATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTcc    <  2:4709871/101‑1 (MQ=255)
                                                                                                   aTAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTcccc  <  2:3373453/101‑1 (MQ=255)
                                                                                                   |                                                                                                    
CCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATGCGGCGATAACTCGCTCTTGCGATCCGTCATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCC  >  NC_014034/1396581‑1396780

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG