Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,816 A→T 15.8% H233L (CAT→CTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8160A→T15.8% 291.3 ‑3.9 ‑0.0615H233L (CAT→CTT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (36/61):  ref base (260/256):  total (296/319)
Fisher's exact test strand distribution p-value = 1.98e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.73e-01

Only 100 of 639 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGC  >  NC_014034/1396716‑1396916
                                                                                                        |                                                                                                    
  gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATg                                                                                                    <  1:6123569/101‑1 (MQ=255)
   cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGc                                                                                                   <  2:3787107/101‑1 (MQ=255)
    gggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCa                                                                                                  <  1:158349/101‑1 (MQ=255)
      gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAgc                                                                                                >  2:8640483/1‑101 (MQ=255)
      gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAgc                                                                                                <  2:7594771/101‑1 (MQ=255)
      gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAgc                                                                                                <  1:2677464/101‑1 (MQ=255)
       ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAgcg                                                                                               <  2:7097784/101‑1 (MQ=255)
          gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCgg                                                                                            <  2:3481944/101‑1 (MQ=255)
            ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGAc                                                                                          >  1:1854631/1‑101 (MQ=255)
             gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACg                                                                                         >  1:453490/1‑101 (MQ=255)
              aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACgg                                                                                        >  1:7418814/1‑101 (MQ=255)
               tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGc                                                                                       <  1:6307630/101‑1 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                                     >  2:4116719/1‑101 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                                     >  1:3753105/1‑101 (MQ=255)
                   aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGCCGGCTTgg                                                                                   >  2:827134/1‑101 (MQ=255)
                      ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                <  1:9138684/101‑1 (MQ=255)
                           cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcc                                                                           <  2:5735185/101‑1 (MQ=255)
                            ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                          <  2:1920724/101‑1 (MQ=255)
                                 tatGCTGCCACGATGCCCTTGAGTCCCCCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                                     <  1:4226030/101‑1 (MQ=255)
                                    gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggg                                                                  >  1:3477266/1‑101 (MQ=255)
                                     ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgggg                                                                 <  2:2302231/101‑1 (MQ=255)
                                      tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                >  1:2420221/1‑101 (MQ=255)
                                      tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                                >  2:8029262/1‑101 (MQ=255)
                                       gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                               <  1:6920456/101‑1 (MQ=255)
                                          aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTg                                                            >  2:9053912/1‑101 (MQ=255)
                                          aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCCGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTg                                                            <  1:4755467/101‑1 (MQ=255)
                                           cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  2:5197562/101‑1 (MQ=255)
                                           cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:7011544/101‑1 (MQ=255)
                                            gATGCCCTTGAGTCGCTCCCGCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  1:7213715/101‑1 (MQ=255)
                                             aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:5421848/101‑1 (MQ=255)
                                             aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  2:2135840/101‑1 (MQ=255)
                                             aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  2:5465232/1‑101 (MQ=255)
                                              tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        >  2:3280588/1‑101 (MQ=255)
                                               gctcTTGAAGCGCTCCCGCCGCGGGGGCGTGGATCGAAACCGTG.TAGAT...GATCATGCAGGGCGGACGGCTTGGGCCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  1:7834293/98‑1 (MQ=37)
                                               gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGTCGTGGATCg                                                       <  2:4709185/101‑1 (MQ=255)
                                                gccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      <  2:4258867/100‑1 (MQ=255)
                                                catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:4350749/94‑1 (MQ=2)
                                                catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:4873732/94‑1 (MQ=2)
                                                 ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  2:7715131/101‑1 (MQ=255)
                                                 ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  1:1003452/1‑101 (MQ=255)
                                                 atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  2:1824412/95‑1 (MQ=2)
                                                  tcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      <  2:1424232/96‑1 (MQ=2)
                                                   ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  2:8832301/101‑1 (MQ=255)
                                                   ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   >  2:5921781/1‑101 (MQ=255)
                                                   ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  2:8494741/101‑1 (MQ=255)
                                                    tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                  >  2:6626252/1‑101 (MQ=255)
                                                    tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                  <  2:1692506/101‑1 (MQ=255)
                                                    gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  1:5059366/98‑1 (MQ=2)
                                                     gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                 >  2:4468054/1‑101 (MQ=255)
                                                     gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                 >  1:2841278/1‑101 (MQ=255)
                                                     gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                 <  1:6717539/101‑1 (MQ=255)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGagatcg                                                 >  1:8847118/1‑96 (MQ=2)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                 >  2:5654099/1‑99 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                               >  2:6533333/1‑97 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                             <  1:8640869/101‑1 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                             >  1:2224630/1‑101 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                             >  2:1191102/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                            >  2:2524060/1‑101 (MQ=255)
                                                          cccccccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggc                                              <  2:2634183/98‑6 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccagatcgg                                           >  2:7253479/1‑94 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGCCCag                                           >  2:3630515/1‑101 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                            >  1:5583578/1‑94 (MQ=2)
                                                             ccccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GAGCATGCGGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                         <  2:6255688/101‑1 (MQ=255)
                                                               ccccGCGGGGGCGTGGATCGAAACTATGCTGGCTC..GACCGAG.GTCACGGCGGGCTATGGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACGgggcgcaggc                                        >  1:8221391/1‑93 (MQ=2)
                                                               ccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                       <  1:5910105/101‑1 (MQ=255)
                                                                cccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                      <  1:994058/101‑1 (MQ=255)
                                                                cccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                      <  1:7107980/101‑1 (MQ=255)
                                                                 ccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                     <  2:2958265/101‑1 (MQ=255)
                                                                    cgGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                  >  1:5789863/1‑101 (MQ=255)
                                                                     gggggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                 >  2:6316160/1‑101 (MQ=255)
                                                                     gggggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                 >  2:4994138/1‑101 (MQ=255)
                                                                     gggggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                 >  1:7496803/1‑101 (MQ=255)
                                                                      ggggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                >  1:7638006/1‑101 (MQ=255)
                                                                      ggggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                >  2:158091/1‑101 (MQ=255)
                                                                          cGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                            <  1:8518689/101‑1 (MQ=255)
                                                                           gTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                           >  1:1108058/1‑101 (MQ=255)
                                                                            tGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                          >  2:3067344/1‑101 (MQ=255)
                                                                            tGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                          <  1:3942366/101‑1 (MQ=255)
                                                                            tGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                          >  1:3609585/1‑101 (MQ=255)
                                                                            tGGATCGAAACCATG.TGGAT...GATCATGCGGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCAGGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                          >  1:6520135/1‑101 (MQ=38)
                                                                             ggATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                         <  2:6079260/101‑1 (MQ=255)
                                                                               aTCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                       <  2:6423977/101‑1 (MQ=255)
                                                                                 cGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                     <  2:7467821/101‑1 (MQ=255)
                                                                                 cGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                     >  1:406052/1‑101 (MQ=255)
                                                                                   aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                   >  2:6856129/1‑101 (MQ=255)
                                                                                   aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                   >  1:2055935/1‑101 (MQ=255)
                                                                                    aaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                  >  2:815350/1‑101 (MQ=255)
                                                                                     aCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa                 >  2:5306853/1‑101 (MQ=255)
                                                                                      ccGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                <  1:6626512/101‑1 (MQ=255)
                                                                                      ccGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                <  1:1337896/101‑1 (MQ=255)
                                                                                         tg.tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc             <  1:3080732/101‑1 (MQ=255)
                                                                                            tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGc           >  1:3232944/1‑101 (MQ=255)
                                                                                            tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGc           >  1:489146/1‑101 (MQ=255)
                                                                                              gat...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc         >  2:6780829/1‑101 (MQ=255)
                                                                                               at...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcc        <  2:7021972/101‑1 (MQ=255)
                                                                                               at...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcc        <  2:8401368/101‑1 (MQ=255)
                                                                                                    gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc      <  1:8346277/101‑1 (MQ=255)
                                                                                                    gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc      <  2:4040306/101‑1 (MQ=255)
                                                                                                      tCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc    <  1:533302/101‑1 (MQ=255)
                                                                                                        aTGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCcgc  >  1:1724697/1‑101 (MQ=255)
                                                                                                        |                                                                                                    
CCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGC  >  NC_014034/1396716‑1396916

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG