BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,937	C→A	14.9%	S273R (AGC→AGA)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,937	0	C→A	14.9%	238.1 ‑1.3 ‑0.1	685	S273R (AGC→AGA)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (40/52): ref base (267/266): total (339/346)
Fisher's exact test strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.46e-01

Only 100 of 726 total aligned reads displayed.


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCG  >  NC_014034/1396837‑1397037


    tCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                           >  1:2791700/1‑101 (MQ=255)


        cccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCg.g                                                                                                      <  1:6826633/101‑1 (MQ=255)


              ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                              <  1:827147/101‑1 (MQ=255)


                ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                            >  1:8461889/1‑101 (MQ=255)


                ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                            <  2:5360711/101‑1 (MQ=255)


                 gCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCgg                                                                                           <  2:4941065/101‑1 (MQ=255)


                   gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.Ct                                                                                        >  1:7046678/1‑101 (MQ=255)


                    tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.Ct.t                                                                                      >  1:4377929/1‑101 (MQ=255)


                    tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACA.GCCT.GCGCG.GC.Cggaatcgcct                                                                                         >  2:7278150/1‑92 (MQ=38)


                    tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.Ct.t                                                                                      >  2:2153281/1‑101 (MQ=255)


                     ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.Tc                                                                                     <  2:75345/101‑1 (MQ=255)


                     ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.Tc                                                                                     >  1:6385334/1‑101 (MQ=255)


                     ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.Tc                                                                                     >  1:2439622/1‑101 (MQ=255)


                       aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCg                                                                                   >  1:7570068/1‑101 (MQ=255)


                          gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.Ct                                                                               <  2:101209/101‑1 (MQ=255)


                           aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTg                                                                              >  1:3776927/1‑101 (MQ=255)


                                ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTg.g                                                                      >  1:5879990/1‑101 (MQ=255)


                                 gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.Gt                                                                     <  1:6221110/101‑1 (MQ=255)


                                    cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGc                                                                  <  1:4493976/101‑1 (MQ=255)


                                         aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcccc                                                             >  2:7447212/1‑101 (MQ=255)


                                            gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                          <  1:659122/101‑1 (MQ=255)


                                               cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                       <  1:8148808/101‑1 (MQ=255)


                                                  gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                    >  2:6556613/1‑101 (MQ=255)


                                                     gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCgg                                                 >  2:1770435/1‑100 (MQ=255)


                                                      ccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                <  2:7637679/100‑1 (MQ=255)


                                                       cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:1837150/101‑1 (MQ=255)


                                                       cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:4828438/101‑1 (MQ=255)


                                                        tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                               >  2:8200495/10‑101 (MQ=0)


                                                        ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                               >  2:6640914/11‑101 (MQ=9)


                                                        attggccccggTCGATCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                              >  1:7177468/11‑101 (MQ=0)


                                                         ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:3930523/92‑1 (MQ=2)


                                                         ttggccccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:4210645/92‑1 (MQ=2)


                                                         gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                              >  1:5561122/10‑101 (MQ=9)


                                                         cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CGG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                              <  1:5134978/93‑1 (MQ=2)


                                                          tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:3962932/93‑1 (MQ=2)


                                                          tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  1:1236750/9‑101 (MQ=2)


                                                          cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:6063172/101‑1 (MQ=255)


                                                          cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:6965279/101‑1 (MQ=255)


                                                          cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  2:8595222/1‑101 (MQ=255)


                                                           ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:3416006/94‑1 (MQ=2)


                                                           ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:7778604/101‑1 (MQ=255)


                                                           cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:4763897/95‑1 (MQ=2)


                                                            tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  2:556132/1‑101 (MQ=255)


                                                            ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:3065514/6‑101 (MQ=2)


                                                            ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGac                                             >  2:2442617/6‑100 (MQ=2)


                                                            ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:3376433/96‑1 (MQ=2)


                                                             gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  1:77640/5‑101 (MQ=2)


                                                             cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:4099670/97‑1 (MQ=2)


                                                              cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  1:174529/97‑1 (MQ=2)


                                                              cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  2:5983736/97‑1 (MQ=2)


                                                              cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  2:5036707/97‑1 (MQ=9)


                                                               tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGcaa                                        >  1:2091687/3‑101 (MQ=2)


                                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  2:6814667/100‑1 (MQ=2)


                                                                ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         >  2:3272903/2‑101 (MQ=2)


                                                                 tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                      >  1:8050018/2‑100 (MQ=9)


                                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                     >  2:5844110/2‑99 (MQ=2)


                                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                     >  1:3423524/2‑99 (MQ=2)


                                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                     >  1:910463/1‑101 (MQ=255)


                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAccc                                     >  2:8765360/1‑99 (MQ=2)


                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATc                                    >  2:7145055/1‑101 (MQ=255)


                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                    >  1:8975475/1‑98 (MQ=2)


                                                                  gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                    <  1:7845048/101‑1 (MQ=255)


                                                                  gTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                     <  1:7344510/101‑3 (MQ=9)


                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                     >  2:672415/1‑100 (MQ=2)


                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                     <  2:5717482/101‑2 (MQ=2)


                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                     <  2:891981/101‑2 (MQ=2)


                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                   <  1:1628002/101‑5 (MQ=2)


                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                  >  2:726021/1‑96 (MQ=2)


                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                 >  1:3249739/1‑101 (MQ=255)


                                                                     ccTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                    <  2:9108524/100‑3 (MQ=2)


                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                   <  1:8538802/101‑1 (MQ=2)


                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCt                                <  2:1399019/101‑1 (MQ=255)


                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                 <  2:2348898/101‑7 (MQ=9)


                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                 >  2:1097683/1‑95 (MQ=9)


                                                                       tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                >  1:2320115/1‑94 (MQ=9)


                                                                       cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                               <  2:4282698/100‑1 (MQ=255)


                                                                       cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                               <  2:359820/100‑1 (MQ=255)


                                                                        ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                              >  2:3111111/1‑92 (MQ=2)


                                                                        ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                               <  2:4510027/101‑9 (MQ=9)


                                                                        ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                               <  2:8300755/101‑9 (MQ=9)


                                                                         cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                <  1:5695275/101‑4 (MQ=2)


                                                                         cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                             >  1:8779430/1‑101 (MQ=255)


                                                                           cccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCCTATGCGC.CGGA.CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACAACaccggggcc                           >  2:2979755/1‑93 (MQ=2)


                                                                            ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                             <  2:3756155/101‑7 (MQ=0)


                                                                               cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                       <  2:1244219/101‑1 (MQ=255)


                                                                               cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGCTgg                       >  2:2633654/1‑101 (MQ=255)


                                                                                gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                      <  1:1942063/101‑1 (MQ=255)


                                                                                  gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                    <  1:1013270/101‑1 (MQ=255)


                                                                                  gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                    >  2:1534138/1‑101 (MQ=255)


                                                                                     cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.GT.TCCGC.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                 >  1:6464517/1‑101 (MQ=255)


                                                                                     cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                 >  1:6688984/1‑101 (MQ=255)


                                                                                      gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                <  1:2698552/101‑1 (MQ=255)


                                                                                       tGGATCGAAACA.GCCT.GCGCG.GC.C.CA.TCGG.CT.TCCGC.CCG..CCGG.GGCGCCCCCCCCGCGGGGGCGGGGGTCGGAACATCAGCTCGGCGTTGGGCAGCCCGa              >  1:1538122/1‑101 (MQ=255)


                                                                                         gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc             >  1:532960/1‑101 (MQ=255)


                                                                                           tCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa           >  2:2015337/1‑101 (MQ=255)


                                                                                             gAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg         >  1:4092171/1‑101 (MQ=255)


                                                                                              aaaCA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt        >  2:8020223/1‑101 (MQ=255)


                                                                                                aCA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc      <  1:4976080/101‑1 (MQ=255)


                                                                                                aCA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc      <  2:6120379/101‑1 (MQ=255)


                                                                                                    gCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc   <  2:6358142/101‑1 (MQ=255)


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCG  >  NC_014034/1396837‑1397037

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG