Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,938 C→T 10.4% M274M (CTG→TTT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9380C→T10.4% 167.2 ‑4.3 ‑0.1686M274M (CTG→TTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (44/23):  ref base (277/283):  total (339/347)
Fisher's exact test strand distribution p-value = 1.38e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.63e-01

Only 100 of 730 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGC  >  NC_014034/1396838‑1397038
                                                                                                     |                                                                                                              
 gcgccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCt                                                                                                               <  2:6382550/98‑1 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                             <  1:3035352/101‑1 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                            >  1:2791700/1‑101 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.Gc                                                                                                      >  2:1213269/1‑101 (MQ=255)
           cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGc                                                                                                  <  2:2426604/101‑1 (MQ=255)
               ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                             <  2:5360711/101‑1 (MQ=255)
                gCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCgg                                                                                            <  2:4941065/101‑1 (MQ=255)
                 cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.c                                                                                          <  1:6686300/101‑1 (MQ=255)
                   tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.Ct.t                                                                                       >  1:4377929/1‑101 (MQ=255)
                   tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.Ct.t                                                                                       >  2:2153281/1‑101 (MQ=255)
                    ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.Tc                                                                                      >  1:6385334/1‑101 (MQ=255)
                    ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.Tc                                                                                      >  1:2439622/1‑101 (MQ=255)
                         gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGc.cc                                                                                >  2:3403766/1‑100 (MQ=255)
                          aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTg                                                                               >  2:1002341/1‑101 (MQ=255)
                             ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGt                                                                          <  1:7147526/101‑1 (MQ=255)
                               ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTg.g                                                                       >  2:6841430/1‑101 (MQ=255)
                               ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTg.g                                                                       >  1:5879990/1‑101 (MQ=255)
                                  ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGC.GGGGCCTGGATCGAAACA.GCCC.GCGCG.GG.CGCC.TCGG.CC.TCCCG.CTG..CGGg.ggccc                                                                   >  2:1361235/1‑97 (MQ=25)
                                   cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGc                                                                   <  1:4493976/101‑1 (MQ=255)
                                      agaTAGACCGCGCGGGCCTCCGCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG.GCCG.GCGCG.GC.CCCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcc                                                                <  1:9082571/101‑1 (MQ=37)
                                      agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCCC.CCG..CGTG.GTCGCTcc                                                                >  1:8952333/1‑101 (MQ=255)
                                        aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcccc                                                              >  2:7447212/1‑101 (MQ=255)
                                          aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                            >  1:7643621/1‑101 (MQ=255)
                                          aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                            <  1:8264603/101‑1 (MQ=255)
                                                 gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                     >  2:6556613/1‑101 (MQ=255)
                                                    gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCCCGGGGGCGt                                                  >  2:3580799/1‑101 (MQ=255)
                                                      ctcgcggttgcGTCGCTCCCCCCGCGGGGGCCTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                 <  1:6584840/91‑1 (MQ=0)
                                                      cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                <  1:4828438/101‑1 (MQ=255)
                                                      cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                <  1:1837150/101‑1 (MQ=255)
                                                       ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                >  2:6640914/11‑101 (MQ=9)
                                                       ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                >  2:106584/11‑101 (MQ=9)
                                                       ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                               >  2:3720304/1‑101 (MQ=255)
                                                       attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                               <  2:3065471/91‑1 (MQ=0)
                                                        ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  1:3930523/92‑1 (MQ=2)
                                                        ttggccccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  1:4210645/92‑1 (MQ=2)
                                                        tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  1:7522927/101‑1 (MQ=255)
                                                        gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                               >  1:5561122/10‑101 (MQ=9)
                                                        cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CGG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                               <  1:5134978/93‑1 (MQ=2)
                                                         tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:3962932/93‑1 (MQ=2)
                                                         tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             >  1:1236750/9‑101 (MQ=2)
                                                         tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:3666637/93‑1 (MQ=2)
                                                         tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  1:3232805/93‑1 (MQ=9)
                                                         cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             >  2:8595222/1‑101 (MQ=255)
                                                         cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:6965279/101‑1 (MQ=255)
                                                         cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:6063172/101‑1 (MQ=255)
                                                          ctcAGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                            <  2:7799474/101‑1 (MQ=255)
                                                          cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:4763897/95‑1 (MQ=2)
                                                           tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           >  2:556132/1‑101 (MQ=255)
                                                           ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  1:3376433/96‑1 (MQ=2)
                                                            cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:4099670/97‑1 (MQ=2)
                                                            cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:2336622/97‑1 (MQ=2)
                                                             cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         <  2:5983736/97‑1 (MQ=2)
                                                             cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         <  1:174529/97‑1 (MQ=2)
                                                             cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:5036707/97‑1 (MQ=9)
                                                              cgggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        >  2:4498074/4‑101 (MQ=2)
                                                               ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:7261113/100‑1 (MQ=2)
                                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                      >  2:5844110/2‑99 (MQ=2)
                                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                      >  1:3423524/2‑99 (MQ=2)
                                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                      <  2:7569744/101‑1 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAccc                                      >  2:8765360/1‑99 (MQ=2)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATc                                     >  2:7145055/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                     <  1:2093368/101‑4 (MQ=2)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                    >  2:1484327/1‑97 (MQ=2)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                    >  2:3266558/1‑97 (MQ=2)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                     >  1:8763255/1‑98 (MQ=9)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                      >  2:672415/1‑100 (MQ=2)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                      <  2:5717482/101‑2 (MQ=2)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                      <  2:891981/101‑2 (MQ=2)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                    <  1:1628002/101‑5 (MQ=2)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                   >  2:19931/1‑96 (MQ=9)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                   >  1:8047849/1‑96 (MQ=9)
                                                                    ccTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                     <  2:9108524/100‑3 (MQ=2)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccac                                 >  1:8429512/1‑94 (MQ=2)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                 >  1:2320115/1‑94 (MQ=9)
                                                                      cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                <  2:4282698/100‑1 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                               >  1:6797494/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                <  2:8300755/101‑9 (MQ=9)
                                                                       ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                <  2:8227756/101‑9 (MQ=9)
                                                                        cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                 <  1:5695275/101‑4 (MQ=2)
                                                                        cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                               <  2:7675210/101‑10 (MQ=9)
                                                                          cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                               >  2:1712587/1‑96 (MQ=0)
                                                                          cccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCCTATGCGC.CGGA.CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACAACaccggggcc                            >  2:2979755/1‑93 (MQ=2)
                                                                           ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                           >  1:812985/1‑101 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGCTgg                        >  2:2633654/1‑101 (MQ=255)
                                                                               gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                       <  1:1942063/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGAA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                       >  1:6989282/1‑101 (MQ=255)
                                                                                ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgggc                      >  2:7279790/1‑99 (MQ=255)
                                                                                 gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                     <  1:1013270/101‑1 (MQ=255)
                                                                                    cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.GT.TCCGC.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                  >  1:6464517/1‑101 (MQ=255)
                                                                                     gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                 <  1:2698552/101‑1 (MQ=255)
                                                                                      tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                >  1:7831947/1‑101 (MQ=255)
                                                                                      tGGATCGAAACA.GCCT.GCGCG.GC.C.CA.TCGG.CT.TCCGC.CCG..CCGG.GGCGCCCCCCCCGCGGGGGCGGGGGTCGGAACATCAGCTCGGCGTTGGGCAGCCCGa               >  1:1538122/1‑101 (MQ=255)
                                                                                        gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc              >  1:532960/1‑101 (MQ=255)
                                                                                           cGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt           <  1:7333994/101‑1 (MQ=255)
                                                                                            gAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg          <  1:8525972/101‑1 (MQ=255)
                                                                                            gAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg          >  1:4092171/1‑101 (MQ=255)
                                                                                             aaaCA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt         >  2:8020223/1‑101 (MQ=255)
                                                                                               aCA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc       <  1:4976080/101‑1 (MQ=255)
                                                                                               aCA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc       <  2:6120379/101‑1 (MQ=255)
                                                                                                cA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg      >  1:6233549/1‑101 (MQ=255)
                                                                                                     |                                                                                                              
CGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGC  >  NC_014034/1396838‑1397038

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG