BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,940	G→T	9.2%	L274L (CTG→TTT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,940	0	G→T	9.2%	144.9 ‑0.1 ‑1.3	676	L274L (CTG→CTT)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (32/31): ref base (304/308): total (336/340)
Fisher's exact test strand distribution p-value = 8.95e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.48e-02

Only 100 of 725 total aligned reads displayed.


CTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTC  >  NC_014034/1396840‑1397040


cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.g                                                                                                              <  1:3035352/101‑1 (MQ=255)


 cccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.gc                                                                                                             <  2:2297897/100‑1 (MQ=255)


             ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCg                                                                                              <  2:6715716/101‑1 (MQ=255)


               cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGc                                                                                            <  1:6686300/101‑1 (MQ=255)


                gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                           >  1:4068063/1‑101 (MQ=255)


                  ggATCGAAACCGGTCCAGAGATAGACCGCGCGGGCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.Tc                                                                                        >  1:6461932/1‑101 (MQ=255)


                  ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GT.CGCA.TCGGCT.Tc                                                                                        >  1:9078144/1‑101 (MQ=255)


                  ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.Tc                                                                                        >  1:5822777/1‑101 (MQ=255)


                  ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.Tc                                                                                        <  2:75345/101‑1 (MQ=255)


                  ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.Tc                                                                                        >  1:2439622/1‑101 (MQ=255)


                        aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTg                                                                                 >  1:3776927/1‑101 (MQ=255)


                         aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                              >  2:3057177/1‑101 (MQ=255)


                           ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..Cgg                                                                            >  2:6937016/1‑100 (MQ=255)


                           ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGt                                                                            <  1:7147526/101‑1 (MQ=255)


                               tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                       <  1:82360/101‑1 (MQ=255)


                                 cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGc                                                                     <  1:4493976/101‑1 (MQ=255)


                                   gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCTGC.CTG..CGTG.GCCGCTc                                                                   >  2:4063273/1‑101 (MQ=255)


                                   gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTc                                                                   >  2:5340527/1‑101 (MQ=255)


                                    agaTAGCCCGCTCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                  <  2:8615460/101‑1 (MQ=255)


                                      aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                >  2:7447212/1‑101 (MQ=255)


                                       tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                               <  2:1033384/101‑1 (MQ=255)


                                         gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                             <  2:1459281/101‑1 (MQ=255)


                                             gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                         <  1:6216117/101‑1 (MQ=255)


                                                cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                      >  1:8714685/1‑101 (MQ=255)


                                                  gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                    <  2:2138065/101‑1 (MQ=255)


                                                   ccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                   <  2:7637679/100‑1 (MQ=255)


                                                    cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:4828438/101‑1 (MQ=255)


                                                    cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:9201562/101‑1 (MQ=255)


                                                    cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  2:3940352/101‑1 (MQ=255)


                                                    cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:1837150/101‑1 (MQ=255)


                                                     ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  2:4642391/91‑1 (MQ=9)


                                                      ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:711445/92‑1 (MQ=2)


                                                      ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:6537636/92‑1 (MQ=2)


                                                      ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:3930523/92‑1 (MQ=2)


                                                      tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                >  1:7828909/1‑101 (MQ=255)


                                                      gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  2:4932210/92‑1 (MQ=9)


                                                      cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CGG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  1:5134978/93‑1 (MQ=2)


                                                       tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               >  1:656916/9‑101 (MQ=2)


                                                       tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               >  1:1236750/9‑101 (MQ=2)


                                                       tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:3232805/93‑1 (MQ=9)


                                                        ctcAGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  2:7799474/101‑1 (MQ=255)


                                                         ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGG.GTCGCTCCCCCCGCGGGGGCGTGGGTCg                                              >  2:5711929/6‑101 (MQ=2)


                                                          ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            >  1:8125942/6‑101 (MQ=2)


                                                          acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  1:8040445/6‑101 (MQ=9)


                                                           cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  1:174529/97‑1 (MQ=2)


                                                           cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  2:580651/97‑1 (MQ=2)


                                                            tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGcaa                                           >  1:2091687/3‑101 (MQ=2)


                                                            tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           >  1:5664195/3‑101 (MQ=2)


                                                            tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           >  1:7480403/3‑101 (MQ=2)


                                                             gcGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACATTGCTA.TGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  2:1359520/100‑1 (MQ=2)


                                                             gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  2:6814667/100‑1 (MQ=2)


                                                             cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         >  2:4495934/3‑100 (MQ=2)


                                                              tgTCGCTCCCCCCGCGGGGGCGTGGGTCGGAAC...GCTG.GGGCGCGC.CGCG.CCGGGTCTTTTT.CTG..CGCG.GTCGCTCCCCCCCCGGGGGCGCGGGTCGAAAcc                                         >  2:2855580/2‑100 (MQ=2)


                                                              tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         <  2:2420206/100‑2 (MQ=9)


                                                              tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         >  1:2773859/2‑100 (MQ=9)


                                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                        >  1:3423524/2‑99 (MQ=2)


                                                              cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  1:6384460/101‑1 (MQ=2)


                                                               ggcGCCCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                       <  2:6639047/99‑1 (MQ=255)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  1:8415003/101‑1 (MQ=2)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAccc                                        >  2:8765360/1‑99 (MQ=2)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATc                                       >  2:3397502/1‑101 (MQ=255)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                       <  1:7274893/101‑1 (MQ=255)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                       <  2:2564852/101‑4 (MQ=2)


                                                               gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                       <  2:4199334/101‑1 (MQ=255)


                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                        <  2:891981/101‑2 (MQ=2)


                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                      <  1:1628002/101‑5 (MQ=2)


                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                     <  1:7620382/101‑6 (MQ=2)


                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                     >  2:726021/1‑96 (MQ=2)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                     <  2:4987521/101‑6 (MQ=2)


                                                                  ccTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                       <  2:9108524/100‑3 (MQ=2)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                    <  2:6348510/101‑7 (MQ=2)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                    <  1:700078/101‑7 (MQ=2)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                   >  1:430382/1‑94 (MQ=9)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                   >  1:2320115/1‑94 (MQ=9)


                                                                     ccccccGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                 >  1:6797494/1‑101 (MQ=255)


                                                                     ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                  <  1:2698517/101‑9 (MQ=9)


                                                                     ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                  >  2:1985070/1‑93 (MQ=9)


                                                                     ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                  <  2:8300755/101‑9 (MQ=9)


                                                                     ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                  <  2:8227756/101‑9 (MQ=9)


                                                                      cccccGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgt                                 <  2:3255287/101‑10 (MQ=2)


                                                                        cccGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                              <  2:5455039/101‑1 (MQ=255)


                                                                          cgcgGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                               <  2:5943052/101‑8 (MQ=0)


                                                                           gcgGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                           <  2:3638506/101‑1 (MQ=255)


                                                                             gggggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                         >  1:8516486/1‑101 (MQ=255)


                                                                             gggggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                         <  1:1942063/101‑1 (MQ=255)


                                                                              ggggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgggc                        >  2:7279790/1‑99 (MQ=255)


                                                                               gggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                       <  1:1013270/101‑1 (MQ=255)


                                                                               gggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                       <  1:6038322/101‑1 (MQ=255)


                                                                               gggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                       <  1:882942/101‑1 (MQ=255)


                                                                                 gCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                     <  1:335921/101‑1 (MQ=255)


                                                                                    tGGATCGAAACA..GCCT.GCGCG.GC.C.CA.TCGGCT.TCCGC.CCG..CCGG.GGCGCCCCCCCCGCGGGGGCGGGGGTCGGAACATCAGCTCGGCGTTGGGCAGCCCGa                 >  1:1538122/1‑101 (MQ=255)


                                                                                     ggATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                 >  2:1651458/1‑101 (MQ=255)


                                                                                      gATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                >  1:532960/1‑101 (MQ=255)


                                                                                       aTCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg               <  2:3515309/101‑1 (MQ=255)


                                                                                           aaaCA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt           >  2:8020223/1‑101 (MQ=255)


                                                                                           aaaCA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt           >  2:1190333/1‑101 (MQ=255)


                                                                                            aaCA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc          >  1:4187229/1‑101 (MQ=255)


                                                                                             aCA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc         <  1:4976080/101‑1 (MQ=255)


                                                                                              cA..GCCG.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg        <  2:607930/101‑1 (MQ=255)


                                                                                                     t.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt   <  1:5514456/101‑1 (MQ=255)


CTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTC  >  NC_014034/1396840‑1397040

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG