BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,947	C→G	16.7%	R277G (CGC→GGT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,947	0	C→G	16.7%	240.4 ‑2.0 ‑1.2	694	R277G (CGC→GGC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (40/53): ref base (247/231): total (339/355)
Fisher's exact test strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.11e-02

Only 100 of 730 total aligned reads displayed.


CGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGC  >  NC_014034/1396847‑1397047


cgcgGGGGCGTGGATCGAAACCGGTCCACAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.Gc.c                                                                                                            <  1:6401186/101‑1 (MQ=255)


     gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.Tc                                                                                                      <  1:4095945/101‑1 (MQ=255)


      ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCg                                                                                                     <  2:6715716/101‑1 (MQ=255)


      ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCg                                                                                                     <  2:5360711/101‑1 (MQ=255)


       gCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCgg                                                                                                    <  2:4941065/101‑1 (MQ=255)


        cGTGGATCGAAACAGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGAGGGGGCGGGGATCGAAACA..GCCT.GCGCG.GA.CGGA.TCGGc                                                                                                   >  2:4218169/1‑101 (MQ=38)


         gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                                  <  1:3783600/101‑1 (MQ=255)


          tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                                <  1:4210677/101‑1 (MQ=255)


           ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.Tc                                                                                               <  2:75345/101‑1 (MQ=255)


                gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                         >  1:4001441/1‑101 (MQ=255)


                    ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGt                                                                                   <  1:7147526/101‑1 (MQ=255)


                     cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTg                                                                                  >  1:9073828/1‑101 (MQ=255)


                      ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGGg.g                                                                                >  2:8228939/1‑101 (MQ=255)


                        tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                              <  1:82360/101‑1 (MQ=255)


                            gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCTGC.CTG..CGTG.GCCGCTc                                                                          >  2:4063273/1‑101 (MQ=255)


                             agaTAGCCCGCTCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                         <  2:8615460/101‑1 (MQ=255)


                                tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                      <  2:1033384/101‑1 (MQ=255)


                                   aCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcgc                                                                   >  1:1941037/1‑101 (MQ=255)


                                    ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcgcg                                                                  >  2:798999/1‑101 (MQ=255)


                                             cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                         <  1:9201562/101‑1 (MQ=255)


                                             cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                         <  2:1908862/101‑1 (MQ=255)


                                               ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                       <  2:6537636/92‑1 (MQ=2)


                                               tcggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                       <  2:3063825/92‑1 (MQ=2)


                                               tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                       >  1:7828909/1‑101 (MQ=255)


                                               cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CGG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                        <  1:5134978/93‑1 (MQ=2)


                                                cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGGG.GTCGCTCCCCCCGCGGGGGGGGGGATc                                                      >  2:2934296/1‑101 (MQ=255)


                                                cctcAGACGTCGCCCCCCCCGCGGGGNNNNNGTTCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                      <  1:4385400/101‑1 (MQ=255)


                                                ccccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                         <  1:1831409/94‑1 (MQ=0)


                                                 gccggtggTCGCTCCCCCCGCGGGGGCGTGGATCGACACA..CCCG.GCGCC.GC.AGCG.AGTGCG.TCTGG.AAC..AGGGTGTCGCTCCCCCCGCCGGGGCGTGGATc                                                      <  1:6512965/94‑1 (MQ=2)


                                                 ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     <  2:7778604/101‑1 (MQ=255)


                                                  tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  2:4765277/1‑101 (MQ=255)


                                                  tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  2:556132/1‑101 (MQ=255)


                                                  ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     >  1:3065514/6‑101 (MQ=2)


                                                  gccccggTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    <  2:8769681/95‑1 (MQ=2)


                                                   ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   >  1:8125942/6‑101 (MQ=2)


                                                   cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   <  1:1611297/101‑1 (MQ=255)


                                                   cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   >  2:8979334/1‑101 (MQ=255)


                                                    cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   <  2:5036707/97‑1 (MQ=9)


                                                     tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGcaa                                                  >  1:2091687/3‑101 (MQ=2)


                                                     tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  1:5664195/3‑101 (MQ=2)


                                                     tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  1:7480403/3‑101 (MQ=2)


                                                     tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGGTCGaaa                                                  >  1:1492067/3‑101 (MQ=2)


                                                     cgggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 >  2:4498074/4‑101 (MQ=2)


                                                     cctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.TCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:7091222/98‑1 (MQ=9)


                                                      gcGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACATTGCTA.TGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  2:1359520/100‑1 (MQ=2)


                                                      gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  2:6814667/100‑1 (MQ=2)


                                                      ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   <  2:7261113/100‑1 (MQ=2)


                                                      aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                >  2:1189224/1‑101 (MQ=255)


                                                      aCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                <  1:6188881/101‑1 (MQ=255)


                                                       tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                <  2:2420206/100‑2 (MQ=9)


                                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                               >  1:173610/2‑99 (MQ=2)


                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  1:8740402/101‑1 (MQ=2)


                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcc                                                >  1:3376552/1‑100 (MQ=2)


                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcc                                                >  1:2312214/1‑100 (MQ=2)


                                                        ggcGCCCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                              <  2:6639047/99‑1 (MQ=255)


                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  1:8415003/101‑1 (MQ=2)


                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATc                                              >  2:3397502/1‑101 (MQ=255)


                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                              <  1:7274893/101‑1 (MQ=255)


                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                              <  1:4579230/101‑1 (MQ=255)


                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActaca                                             >  2:7627398/1‑97 (MQ=2)


                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                             >  2:3266558/1‑97 (MQ=2)


                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                               <  2:5717482/101‑2 (MQ=2)


                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                            <  1:7620382/101‑6 (MQ=2)


                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                            >  1:3523817/1‑96 (MQ=2)


                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                             >  2:3942392/1‑97 (MQ=9)


                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                           >  1:3249739/1‑101 (MQ=255)


                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                           <  1:5812228/101‑1 (MQ=255)


                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                           >  2:1462616/1‑95 (MQ=2)


                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                           <  2:6348510/101‑7 (MQ=2)


                                                            cTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                           >  1:2771859/1‑95 (MQ=9)


                                                              ccccccGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                        >  2:8384948/1‑92 (MQ=2)


                                                              ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                         >  2:9112219/1‑93 (MQ=9)


                                                              ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                         >  1:2659623/1‑93 (MQ=9)


                                                               cccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                        <  2:2632551/101‑10 (MQ=9)


                                                                ccccGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                      <  1:6785191/101‑1 (MQ=255)


                                                                 cccGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                     >  1:4839196/1‑101 (MQ=255)


                                                                   cgcgGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                      <  2:5943052/101‑8 (MQ=0)


                                                                    gcgGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                  <  2:3638506/101‑1 (MQ=255)


                                                                      gggggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                >  1:8516486/1‑101 (MQ=255)


                                                                      gggggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGAA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                >  1:6989282/1‑101 (MQ=255)


                                                                       ggggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgggc                               >  2:7279790/1‑99 (MQ=255)


                                                                        gggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                              <  1:1013270/101‑1 (MQ=255)


                                                                        gggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                              <  1:6617211/101‑1 (MQ=255)


                                                                        gggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                              <  1:882942/101‑1 (MQ=255)


                                                                            gTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                          <  2:6931857/101‑1 (MQ=255)


                                                                             gggATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                         <  2:7419924/100‑1 (MQ=255)


                                                                              ggATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                        >  2:1651458/1‑101 (MQ=255)


                                                                              ggATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                        >  1:6358391/1‑101 (MQ=255)


                                                                               gATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                       >  2:2279415/1‑101 (MQ=255)


                                                                               gATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                       >  1:532960/1‑101 (MQ=255)


                                                                                aTCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                      <  2:3515309/101‑1 (MQ=255)


                                                                                  cGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                    >  1:2434568/1‑101 (MQ=255)


                                                                                     atcA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                 <  1:5986429/99‑1 (MQ=255)


                                                                                      aCA..GCCT.GCACG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                >  2:2102330/1‑101 (MQ=255)


                                                                                       cA..GCCG.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg               <  2:607930/101‑1 (MQ=255)


                                                                                        a..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGt              <  1:8023462/101‑1 (MQ=255)


                                                                                              t.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt          <  1:5514456/101‑1 (MQ=255)


                                                                                                   cg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccc      >  1:1227867/1‑101 (MQ=255)


                                                                                                    g.gC.CTCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc     <  2:5822542/101‑1 (MQ=255)


                                                                                                       c.cGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcg   >  1:4986870/1‑101 (MQ=255)


CGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGC  >  NC_014034/1396847‑1397047

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG