Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,958 C→A 18.7% F280L (TTC→TTA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9580C→A18.7% 264.1 ‑1.0 ‑0.1690F280L (TTC→TTARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (39/54):  ref base (195/213):  total (330/360)
Fisher's exact test strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.37e-01

Only 100 of 729 total aligned reads displayed.

GGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1396858‑1397058
                                                                                                          |                                                                                                          
ggATCGAAACCGGTCCAGAGATAGACCGCGCGGTCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.Tc                                                                                                            >  1:6438986/1‑101 (MQ=255)
  aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCg                                                                                                          <  2:6797303/101‑1 (MQ=255)
      aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTg                                                                                                   >  2:1002341/1‑101 (MQ=255)
        aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..Cg                                                                                               <  1:5249878/101‑1 (MQ=255)
              ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCg                                                                                        >  2:8731556/1‑101 (MQ=255)
              ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCg                                                                                        >  1:2550256/1‑101 (MQ=255)
               cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGc                                                                                       <  1:4493976/101‑1 (MQ=255)
                  agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTcc                                                                                    <  2:453482/101‑1 (MQ=255)
                  agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTcc                                                                                    >  1:2394136/1‑101 (MQ=255)
                   gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCC..C.CTC..CGTG.GTCGCTccc                                                                                   >  1:2231997/1‑101 (MQ=255)
                    aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTcccc                                                                                  >  1:5028830/1‑101 (MQ=255)
                    aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTcccc                                                                                  >  2:3883614/1‑101 (MQ=255)
                        aCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCcgc                                                                              >  1:7414099/1‑101 (MQ=255)
                              cgGCCCCCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                        <  1:5678257/101‑1 (MQ=255)
                              cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                        <  2:4001326/101‑1 (MQ=255)
                                gACCTCCTCAGACGTCGCTCCCCCCGCGTGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                                      >  1:7577763/1‑101 (MQ=255)
                                 aCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                     >  1:6928251/1‑101 (MQ=255)
                                  cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:4271586/1‑101 (MQ=255)
                                   tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT..C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  2:8200495/10‑101 (MQ=0)
                                    gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG..T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  2:4932210/92‑1 (MQ=9)
                                     tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG..CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  1:1236750/9‑101 (MQ=2)
                                     tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG..CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  2:5773748/93‑1 (MQ=2)
                                      cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT..C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  1:840943/7‑101 (MQ=2)
                                      cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT..C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  1:1694852/95‑1 (MQ=2)
                                       tcAGACGTCGCTGCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               >  1:8079747/1‑101 (MQ=255)
                                       ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG..C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:6397868/96‑1 (MQ=2)
                                        gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT..C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               <  1:6315184/97‑1 (MQ=2)
                                        cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              <  2:3042734/101‑1 (MQ=255)
                                        cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  2:8538471/1‑101 (MQ=255)
                                         cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG..T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  2:7072468/5‑101 (MQ=9)
                                         cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG..T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  2:7774884/5‑101 (MQ=9)
                                           aCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           <  1:6188881/101‑1 (MQ=255)
                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.AGTGCG.TCTGGAA.CAG..GATG.G.CGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                           >  1:3386243/2‑100 (MQ=2)
                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG..CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                          >  2:5844110/2‑99 (MQ=2)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG..C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             >  1:18851/1‑101 (MQ=2)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcct                                                          <  1:8780067/101‑3 (MQ=255)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                          >  1:910463/1‑101 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT..C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                          >  1:7982959/1‑99 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                         >  1:4856249/1‑101 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG..CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                         <  2:5950203/101‑4 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG..CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                         >  1:8228048/1‑98 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG..CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                         >  1:8975475/1‑98 (MQ=2)
                                              gcGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                        <  2:3609477/100‑1 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG..C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                          <  2:891981/101‑2 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG..CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                                       >  1:8508117/1‑96 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT..C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                       <  2:4987521/101‑6 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                      <  1:5812228/101‑1 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                      >  1:3249739/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                      >  2:7247081/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                      >  2:2163257/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG..T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                       >  2:19931/1‑96 (MQ=9)
                                                ccttcccccGCGGGGGCGTGTATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TA..C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                       <  2:5350957/97‑6 (MQ=2)
                                                ccTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG..C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                         <  2:9108524/100‑3 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG..C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                        >  1:3131852/1‑101 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG..C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                        <  2:5619796/101‑1 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG..CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                                     <  1:2000097/101‑8 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG..T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                                      >  2:1097683/1‑95 (MQ=9)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                    >  2:1412237/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                    >  2:6569527/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG..CGCTGGACATG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                                    <  2:4368828/101‑9 (MQ=0)
                                                   ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                   >  1:6797494/1‑101 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG..T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                    <  2:8300755/101‑9 (MQ=9)
                                                   ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG..T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                    <  1:4730383/101‑9 (MQ=9)
                                                    cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                                  <  1:1767624/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG..C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                                   >  2:1712587/1‑96 (MQ=0)
                                                      cccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                                <  2:7522851/101‑1 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG..C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                  <  2:3241074/101‑7 (MQ=0)
                                                          cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                            <  2:1244219/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                           <  1:158091/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                           <  2:335958/101‑1 (MQ=255)
                                                            ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgggc                                          >  2:7279790/1‑99 (MQ=255)
                                                            ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                                          <  2:2394063/101‑1 (MQ=255)
                                                             gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                         <  1:1013270/101‑1 (MQ=255)
                                                             gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                         <  1:6617211/101‑1 (MQ=255)
                                                             gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                         >  2:1534138/1‑101 (MQ=255)
                                                               gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCATCAGCTCGTCGTTGGCCAGc                                       >  1:2808762/1‑101 (MQ=255)
                                                                 gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                     >  2:3449850/1‑101 (MQ=255)
                                                                 gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                     <  1:2698552/101‑1 (MQ=255)
                                                                  tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                    <  1:5355503/101‑1 (MQ=255)
                                                                   ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCCGCCGGa                                   >  2:6146303/1‑101 (MQ=255)
                                                                   ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGGCAGCCGGa                                   >  2:6696335/1‑101 (MQ=255)
                                                                       cGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                               <  1:7777412/101‑1 (MQ=255)
                                                                         aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                             >  1:3574573/1‑101 (MQ=255)
                                                                          aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                            >  1:4187229/1‑101 (MQ=255)
                                                                                 cT.GCGCG.GC.CGCC.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                      >  1:6550690/1‑101 (MQ=255)
                                                                                    gcgcG.GC.CGCA.TCGGCT.TCCT..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc                    >  1:7259857/1‑101 (MQ=255)
                                                                                    gcgcG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc                    <  1:404617/101‑1 (MQ=255)
                                                                                      gcg.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc                  <  2:6927966/101‑1 (MQ=255)
                                                                                        g.gC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc                <  1:556142/101‑1 (MQ=255)
                                                                                          gC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc               <  2:7609490/101‑1 (MQ=255)
                                                                                          gC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc               <  2:2738393/101‑1 (MQ=255)
                                                                                           c.cGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcg              <  2:7418490/101‑1 (MQ=255)
                                                                                             cGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc             <  1:6016707/101‑1 (MQ=255)
                                                                                             cGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc             <  2:4790656/101‑1 (MQ=255)
                                                                                              gCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg            <  1:7139032/101‑1 (MQ=255)
                                                                                                a.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg          >  1:385417/1‑101 (MQ=255)
                                                                                                    ggCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc       <  2:414125/101‑1 (MQ=255)
                                                                                                      cT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAACATCAGCTCCTCGCTGGTCAGCCGGGCGATGGCCGTCGCCCCCCCCCCgggggggg     >  2:2563821/1‑98 (MQ=25)
                                                                                                       t.tCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTg    <  2:7910444/101‑1 (MQ=255)
                                                                                                          ccG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGa  <  1:8681937/101‑1 (MQ=255)
                                                                                                          |                                                                                                          
GGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG..C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1396858‑1397058

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG