Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,959 C→T 32.4% R281C (CGC→TTC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9590C→T32.4% 0.0 ‑2.4 ‑0.0689R281C (CGC→TGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (117/106):  ref base (211/254):  total (329/360)
Fisher's exact test strand distribution p-value = 8.71e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 723 total aligned reads displayed.

GATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1396859‑1397059
                                                                                                           |                                                                                                        
   cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGc.c                                                                                                      <  2:7516233/101‑1 (MQ=255)
    gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGc.cc                                                                                                     >  2:3403766/1‑100 (MQ=255)
     aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCT.GC.CGCA.TCGGCT.TCCGC.CTg                                                                                                    >  1:7241645/1‑101 (MQ=255)
     aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTg                                                                                                    >  2:1002341/1‑101 (MQ=255)
     aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTg                                                                                                    >  1:1661907/1‑101 (MQ=255)
       aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..Cg                                                                                                >  1:4839554/1‑101 (MQ=255)
             ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCg                                                                                         >  2:8731556/1‑101 (MQ=255)
             ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCg                                                                                         >  1:2550256/1‑101 (MQ=255)
             ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.CCGGCT.TCCCC.CCG..ccgggccc                                                                                          >  2:4975920/1‑94 (MQ=37)
                 agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCCC.CCG..CGTG.GTCGCTcc                                                                                     >  1:8952333/1‑101 (MQ=255)
                     aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                 >  1:2164476/1‑101 (MQ=255)
                     aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                 <  2:7914570/101‑1 (MQ=255)
                        ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcgcg                                                                              <  1:1974533/101‑1 (MQ=255)
                               gACCTCCTCAGACGTCGCTCCCCCCGCGTGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                                       >  1:7577763/1‑101 (MQ=255)
                                aCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                      >  1:6928251/1‑101 (MQ=255)
                                aCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                      <  1:3977567/101‑1 (MQ=255)
                                 cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  1:6392546/101‑1 (MQ=255)
                                  tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  2:8200495/10‑101 (MQ=0)
                                  ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  2:106584/11‑101 (MQ=9)
                                  ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                    <  1:8321016/101‑1 (MQ=255)
                                  ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.ATG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                    >  1:4990518/1‑101 (MQ=255)
                                  attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                    >  2:7066877/11‑101 (MQ=0)
                                   ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  2:6537636/92‑1 (MQ=2)
                                   gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                    >  1:1879661/10‑101 (MQ=9)
                                   gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                    >  2:303996/10‑101 (MQ=9)
                                    tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  2:3962932/93‑1 (MQ=2)
                                    tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  1:3232805/93‑1 (MQ=9)
                                    cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  >  2:8595222/1‑101 (MQ=255)
                                     ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  2:7778604/101‑1 (MQ=255)
                                     cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  2:4763897/95‑1 (MQ=2)
                                      ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  1:3376433/96‑1 (MQ=2)
                                       cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  2:4099670/97‑1 (MQ=2)
                                        cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              <  2:3201197/97‑1 (MQ=2)
                                        cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  2:6787282/5‑101 (MQ=9)
                                         tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              >  1:5664195/3‑101 (MQ=2)
                                         cgggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             >  2:4498074/4‑101 (MQ=2)
                                           tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                            >  1:7955316/2‑100 (MQ=9)
                                           tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                            >  1:2773859/2‑100 (MQ=9)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcct                                                           <  1:8780067/101‑3 (MQ=255)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                           <  1:5923102/101‑1 (MQ=255)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                           >  1:910463/1‑101 (MQ=255)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGTAACA..ACT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                          <  1:1752065/101‑4 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             <  1:5325900/101‑1 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaacc                                                           >  2:3330347/1‑98 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                          >  1:8228048/1‑98 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                           >  1:576239/1‑99 (MQ=9)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                                          >  2:2541281/1‑98 (MQ=2)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                         <  1:8503765/101‑1 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                           <  2:891981/101‑2 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                       >  2:2163257/1‑101 (MQ=255)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                        >  2:19931/1‑96 (MQ=9)
                                               ccTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                          <  2:9108524/100‑3 (MQ=2)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                       <  2:6348510/101‑7 (MQ=2)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                     >  2:1412237/1‑101 (MQ=255)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                      >  1:2320115/1‑94 (MQ=9)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                      >  2:5727446/1‑94 (MQ=9)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                      >  2:7237235/1‑94 (MQ=9)
                                                 cccccccGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                     <  2:4282698/100‑1 (MQ=255)
                                                 cccccccGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                     <  2:359820/100‑1 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                       >  1:5499544/1‑99 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                       <  2:6103686/101‑3 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                    >  1:6797494/1‑101 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACA..ACT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                    <  1:8592940/101‑10 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                     <  2:2371519/101‑9 (MQ=9)
                                                  ccccccGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                     >  1:3481028/1‑93 (MQ=9)
                                                  ccccccGCGGGGGCGTGGATCGAAAC..GCTG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                     <  2:8300755/101‑9 (MQ=9)
                                                   cccccGCGGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                                      <  1:6156805/101‑4 (MQ=2)
                                                   cccccGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                                   <  1:4250481/101‑1 (MQ=255)
                                                    ccccGCGGGGGCGTGGATCGAAACATGGCT.GTGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                                                   <  1:3793565/101‑11 (MQ=2)
                                                     cccGCGGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                                    <  2:4897259/101‑6 (MQ=0)
                                                     cccGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                                 <  1:4468189/101‑1 (MQ=255)
                                                     cccGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                                 <  2:5455039/101‑1 (MQ=255)
                                                       cgcgGGGGCGTGGATCGAAACT.GCGT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                  <  2:5943052/101‑8 (MQ=0)
                                                         cgGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                             <  2:1244219/101‑1 (MQ=255)
                                                            gggCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                          <  1:1013270/101‑1 (MQ=255)
                                                            gggCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                          >  2:1534138/1‑101 (MQ=255)
                                                            gggCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                          <  1:6617211/101‑1 (MQ=255)
                                                               cGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CCG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTGGTCAgac                                       >  1:6280607/1‑99 (MQ=255)
                                                                gTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                      <  1:2698552/101‑1 (MQ=255)
                                                                gTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                      <  1:8060737/101‑1 (MQ=255)
                                                                 tGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                     >  2:5294870/1‑101 (MQ=255)
                                                                 tGGATCGAAACA.GCCT.G.CGCG.GC.C.CA.TCGGCT.TCCGC.CCG..CCGG.GGCGCCCCCCCCGCGGGGGCGGGGGTCGGAACATCAGCTCGGCGTTGGGCAGCCCGa                                    >  1:1538122/1‑101 (MQ=255)
                                                                    aTCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                                  <  2:7270999/101‑1 (MQ=255)
                                                                      cGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                <  1:7777412/101‑1 (MQ=255)
                                                                       gAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCCTTGGTCAGGCGGACGCTg                               >  2:3744144/1‑101 (MQ=255)
                                                                        aaaCA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TTCGC.CTG..CGTG.GTTGCTGCCCTCGCGGGGGCGTGGATCTAATCATCCCCTCGTCCTTGTTCAGCCgtggcaggt                              >  2:6914627/1‑93 (MQ=255)
                                                                        aaaCA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                              <  2:6673648/101‑1 (MQ=255)
                                                                              gCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                         <  1:4118707/101‑1 (MQ=255)
                                                                                cT.G.CGCG.GC.CGCC.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                       >  1:6550690/1‑101 (MQ=255)
                                                                                       cg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccc                  >  1:1227867/1‑101 (MQ=255)
                                                                                        g.gC.CTCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc                 <  2:5822542/101‑1 (MQ=255)
                                                                                          gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc                >  1:7392731/1‑101 (MQ=255)
                                                                                          gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc                <  2:2738393/101‑1 (MQ=255)
                                                                                          gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc                <  2:7609490/101‑1 (MQ=255)
                                                                                           c.cGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcg               <  2:7418490/101‑1 (MQ=255)
                                                                                             cGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc              <  2:166458/101‑1 (MQ=255)
                                                                                              gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg             <  1:7139032/101‑1 (MQ=255)
                                                                                                  tCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgggg          <  1:3057276/101‑1 (MQ=255)
                                                                                                       t.tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTg     <  1:4713839/101‑1 (MQ=255)
                                                                                                         tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTgg    <  1:5202468/101‑1 (MQ=255)
                                                                                                           |                                                                                                        
GATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1396859‑1397059

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG