Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,014 T→A 16.9% L299* (TTG→TAG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,0140T→A16.9% 268.3 ‑3.7 ‑0.9579L299* (TTG→TAG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (30/57):  ref base (216/233):  total (280/299)
Fisher's exact test strand distribution p-value = 2.51e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.16e-01

Only 100 of 601 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGC  >  NC_014034/1396914‑1397113
                                                                                                     |                                                                                                        
 gcgGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTg                                                                                                         <  2:9073406/101‑1 (MQ=255)
  cgGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGCTgg                                                                                                        >  2:2633654/1‑101 (MQ=255)
     gggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                                                                     <  1:6038322/101‑1 (MQ=255)
        cGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.Gcc                                                                                                 >  1:6688984/1‑101 (MQ=255)
        cGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.Gcc                                                                                                 >  2:8681552/1‑101 (MQ=255)
          tGGATCGAAACAGCCTGCGCGGCC.CATCGGCTTCCGCCCGCCGGGGCGCCCCCCCCGCGGGGGCGGGGGTCGGAACATCAGCTCG.GCGTTGGGCA.GCCCGa                                                                                              >  1:1538122/1‑101 (MQ=255)
           ggATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGa                                                                                              >  2:1651458/1‑101 (MQ=255)
           ggATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGGCA.GCCGGa                                                                                              >  2:6696335/1‑101 (MQ=255)
               cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GAt                                                                                         <  1:7777412/101‑1 (MQ=255)
               cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GAt                                                                                         <  1:7333994/101‑1 (MQ=255)
               cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GAt                                                                                         <  2:5603837/101‑1 (MQ=255)
                gAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATg                                                                                        >  2:6016472/1‑101 (MQ=255)
                gAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCCTTGGTCA.GGCGGAC.GCTg                                                                                        >  2:3744144/1‑101 (MQ=255)
                 aaaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGt                                                                                       >  1:1892150/1‑101 (MQ=255)
                   aCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTcc                                                                                     <  2:6120379/101‑1 (MQ=255)
                     aGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...Gt                                                                                <  1:8023462/101‑1 (MQ=255)
                        gggcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGc                                                                             >  2:3367182/3‑101 (MQ=255)
                        cTGCGCGGCCGCCTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGc                                                                             >  1:6550690/1‑101 (MQ=255)
                         tGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCt                                                                            <  1:5514456/101‑1 (MQ=255)
                         tGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCt                                                                            <  2:4067948/101‑1 (MQ=255)
                            gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTccc                                                                         <  2:6927966/101‑1 (MQ=255)
                             cgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTcccc                                                                        <  2:8509382/101‑1 (MQ=255)
                              ggCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTccccc                                                                       <  2:5823041/101‑1 (MQ=255)
                                ccGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCcg                                                                     >  1:1590511/1‑101 (MQ=255)
                                ccGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCcg                                                                     >  1:6176697/1‑101 (MQ=255)
                                ccGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCcg                                                                     >  1:4986870/1‑101 (MQ=255)
                                    aTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCggg                                                                 >  1:385417/1‑101 (MQ=255)
                                      cGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCggggg                                                               <  2:7375655/101‑1 (MQ=255)
                                       ggCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGc                                                              <  2:4839394/101‑1 (MQ=255)
                                          ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                           >  1:4662610/1‑101 (MQ=255)
                                           tCCGGCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                          <  2:1328537/101‑1 (MQ=255)
                                           tCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                          >  1:575817/1‑101 (MQ=255)
                                           tCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                          >  2:7501099/1‑101 (MQ=255)
                                            ggatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTg                                                           <  1:5195271/91‑1 (MQ=2)
                                            ggatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTg                                                           >  1:716007/11‑101 (MQ=2)
                                             cGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  1:3020364/101‑1 (MQ=255)
                                              gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  2:677068/101‑1 (MQ=255)
                                              ctggacGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGG.GCTCTGGTCACGGTTTGC.GACG.GA...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  1:4776140/96‑1 (MQ=0)
                                               tcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  1:8515331/94‑1 (MQ=2)
                                               tcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  1:4309583/94‑1 (MQ=2)
                                               tcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  2:180247/94‑1 (MQ=2)
                                               ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACA.GCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        >  1:8268584/9‑101 (MQ=2)
                                               ccTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  2:460725/1‑101 (MQ=255)
                                                cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  1:1149375/95‑1 (MQ=2)
                                                cccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  1:1264129/95‑1 (MQ=2)
                                                 ccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      <  2:7796954/96‑1 (MQ=2)
                                                 agcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACA.GCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  1:3516000/7‑101 (MQ=2)
                                                  gCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  1:6862585/101‑1 (MQ=255)
                                                  gCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  2:7084878/101‑1 (MQ=255)
                                                    gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                 <  2:6519875/101‑1 (MQ=255)
                                                    ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  2:2038052/99‑1 (MQ=2)
                                                     ttgTCGCACCCCCCGCGGGGGCGTGGACCGAAACATCAGCTCC.TCGTTGGTCT.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                <  1:240367/99‑1 (MQ=37)
                                                     tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                >  2:1550261/1‑101 (MQ=255)
                                                     cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                  <  2:3934611/100‑1 (MQ=2)
                                                      ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                               >  2:7885939/1‑101 (MQ=255)
                                                      cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACA.GCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                 >  1:2543574/2‑100 (MQ=2)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACA.GCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaa                                                >  2:4944464/1‑99 (MQ=2)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCg                                             >  2:8306648/1‑101 (MQ=255)
                                                        tCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACA.GCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaac                                               <  2:2499022/101‑4 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcg                                              >  2:3077136/1‑100 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                            >  1:3179930/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACA.GCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                             >  1:996200/1‑96 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCt                                          <  1:8595607/101‑1 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACA.GCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactga                                            >  2:3264180/1‑95 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                           <  1:1009362/101‑5 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                           >  1:7099793/1‑97 (MQ=2)
                                                             ccccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                          <  1:213551/101‑6 (MQ=2)
                                                             ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                        <  2:8951912/101‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                        <  2:8079398/101‑1 (MQ=255)
                                                               ccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAg                                      >  1:8786156/1‑101 (MQ=255)
                                                               ccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAg                                      >  2:5098177/1‑101 (MQ=255)
                                                                cccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatct                                       <  1:5640869/101‑9 (MQ=2)
                                                                cccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                     <  1:3403870/101‑1 (MQ=255)
                                                                 ccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatctc                                      <  1:3990648/101‑10 (MQ=2)
                                                                 ccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatctc                                      >  1:44520/1‑92 (MQ=2)
                                                                   gcgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGCGTGAt                                  >  2:3608066/1‑101 (MQ=255)
                                                                    cgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATg                                 <  1:4063388/101‑1 (MQ=255)
                                                                      ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                               <  2:2808663/101‑1 (MQ=255)
                                                                        ggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgc                             <  1:3630623/101‑1 (MQ=255)
                                                                           gTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                          >  2:1941506/1‑101 (MQ=255)
                                                                            tGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa                         >  1:5905638/1‑101 (MQ=255)
                                                                               aTCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGca                      >  2:3455143/1‑101 (MQ=255)
                                                                                tCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                     <  1:6781100/101‑1 (MQ=255)
                                                                                  gAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                   <  2:2292373/101‑1 (MQ=255)
                                                                                   aaaCATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                  >  1:2772373/1‑101 (MQ=255)
                                                                                        tCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgtc             >  2:6280266/1‑101 (MQ=255)
                                                                                         cAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGtcgtcg            >  2:5897028/1‑101 (MQ=255)
                                                                                            cTCG.TCGTTGGTCA.GCCTGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTc         >  1:6311732/1‑101 (MQ=255)
                                                                                            cTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCCCGACCACATgtcctgcctc         >  2:4171149/1‑94 (MQ=37)
                                                                                              cg.tcgtTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc       >  1:5316866/1‑101 (MQ=255)
                                                                                              cg.tcgtGGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGACCACATGTCGTCGCTccc       <  2:7202127/101‑1 (MQ=255)
                                                                                                 tcgtTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc     <  1:332857/101‑1 (MQ=255)
                                                                                                 tcgtTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc     <  2:4373766/101‑1 (MQ=255)
                                                                                                 tcgtTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc     >  2:6413751/1‑101 (MQ=255)
                                                                                                 tcgtTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc     <  1:749232/101‑1 (MQ=255)
                                                                                                  cgtTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc    >  2:5416706/1‑101 (MQ=255)
                                                                                                   gtTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCTCCcg   <  1:8979755/101‑1 (MQ=255)
                                                                                                   gtTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcg   >  1:4163967/1‑101 (MQ=255)
                                                                                                    ttGGTCA.GCCGGCC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgc  >  1:8163436/1‑101 (MQ=255)
                                                                                                    ttGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgc  <  2:2420148/101‑1 (MQ=255)
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CGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCA.GCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGC  >  NC_014034/1396914‑1397113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG