Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,066 T→G 7.6% T316T (ACT→ACG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,0660T→G 7.6% 77.3 ‑1.7 ‑0.9495T316T (ACT→ACGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/23):  ref base (236/221):  total (251/244)
Fisher's exact test strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.20e-01

Only 100 of 517 total aligned reads displayed.

GTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCC  >  NC_014034/1396966‑1397166
                                                                                                      |                                                                                                    
gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACt                                                                                                      >  1:4290046/1‑101 (MQ=255)
gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                      >  1:3945268/1‑101 (MQ=255)
 tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                                                                     >  2:1550261/1‑101 (MQ=255)
  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                    >  2:4706444/1‑101 (MQ=255)
  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                    >  1:3246545/1‑101 (MQ=255)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcc                                                                                                   <  1:1534185/101‑1 (MQ=255)
    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGc                                                                                                    <  2:2006173/101‑1 (MQ=2)
    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGc                                                                                                    >  1:3814493/1‑101 (MQ=2)
     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcg                                                                                                   >  1:5591983/1‑100 (MQ=2)
     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                                                                                 >  1:6042138/1‑101 (MQ=255)
     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                                                                                 >  1:4054362/1‑101 (MQ=255)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgg                                                                                                  >  1:3063924/1‑99 (MQ=2)
       ccccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCt                                                                                               <  1:8515024/99‑1 (MQ=255)
       cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCt                                                                                               <  2:6464268/101‑1 (MQ=255)
        tCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                                                                                <  1:1009362/101‑5 (MQ=2)
        cccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                                                                              <  1:1770488/100‑1 (MQ=255)
         ccccgcGCGGTGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGCAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                                                                             <  2:2605406/101‑1 (MQ=255)
         ccccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                                                                               >  2:1484314/1‑96 (MQ=2)
           ccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAg                                                                                           <  1:3685809/101‑1 (MQ=255)
           ccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAg                                                                                           >  1:3456119/1‑101 (MQ=255)
           ccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAg                                                                                           <  1:159873/101‑1 (MQ=255)
               gcgGGGGCGTGGATCGAAACATCAACTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGAt                                                                                       >  2:4370844/1‑101 (MQ=255)
                cgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATg                                                                                      >  2:5459384/1‑101 (MQ=255)
                cgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATg                                                                                      >  1:7150121/1‑101 (MQ=255)
                 gggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                                                                     <  1:3400152/101‑1 (MQ=255)
                 gggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                                                                     <  2:3882676/101‑1 (MQ=255)
                 gggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                                                                     <  2:708477/101‑1 (MQ=255)
                      cGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcgg                                                                                >  2:1342810/1‑100 (MQ=255)
                      cGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcgc                                                                                <  2:365371/101‑1 (MQ=255)
                       gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                                                                               >  2:1941959/1‑101 (MQ=255)
                       gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                                                                               >  2:7830579/1‑101 (MQ=255)
                       gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                                                                               >  2:8251132/1‑101 (MQ=255)
                       gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                                                                               <  2:3014559/101‑1 (MQ=255)
                        tGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa                                                                              >  1:5905638/1‑101 (MQ=255)
                         ggATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                                                                             >  2:5646514/1‑101 (MQ=255)
                          gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                                                            <  2:6797223/101‑1 (MQ=255)
                           aTCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGca                                                                           <  2:4187111/101‑1 (MQ=255)
                            tCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                                                                          <  2:3182933/101‑1 (MQ=255)
                             cGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcaca                                                                         <  2:4990358/101‑1 (MQ=255)
                              gAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                                                                        >  1:7751661/1‑101 (MQ=255)
                               aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                                                                       >  1:8556471/1‑101 (MQ=255)
                                 aCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc                                                                     <  1:1213301/101‑1 (MQ=255)
                                 aCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc                                                                     >  2:6636895/1‑101 (MQ=255)
                                  cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg                                                                    >  1:510123/1‑101 (MQ=255)
                                  cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGGGGACCAAACCTGCGGCCTTGGGGGAGGGGGGGGGAGACCAGgtcg                                                                    >  1:6756735/1‑101 (MQ=19)
                                   aTCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgt                                                                   >  2:8567564/1‑101 (MQ=255)
                                    tCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgtc                                                                  >  2:1720340/1‑101 (MQ=255)
                                     cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGtcgtcg                                                                 <  2:8520881/101‑1 (MQ=255)
                                      aGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGc                                                                <  1:5678280/101‑1 (MQ=255)
                                       gCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt                                                               >  2:6101161/1‑101 (MQ=255)
                                       gCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt                                                               >  2:5986330/1‑101 (MQ=255)
                                        cTCG.TCGTTGGTCAGCCTGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTc                                                              >  1:6311732/1‑101 (MQ=255)
                                        cTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTc                                                              >  1:4123467/1‑101 (MQ=255)
                                          cg.tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc                                                            >  2:7165474/1‑101 (MQ=255)
                                          cg.tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc                                                            >  2:7430677/1‑101 (MQ=255)
                                           g.tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccc                                                           <  2:7643294/101‑1 (MQ=255)
                                           g.tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccc                                                           >  1:7930744/1‑101 (MQ=255)
                                             tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc                                                          <  1:3608174/101‑1 (MQ=255)
                                             tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc                                                          <  1:749232/101‑1 (MQ=255)
                                                ttGGTCAGCCGGCC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgc                                                       >  1:8163436/1‑101 (MQ=255)
                                                        ccGGAC.GATGTCCGTCGCTCGCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGt                                               <  1:7247385/101‑1 (MQ=255)
                                                          ggAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTgg                                             >  1:831373/1‑101 (MQ=255)
                                                          ggAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCCCCGGGGCGTgg                                             >  2:8398842/1‑101 (MQ=255)
                                                           gAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTggc                                            >  1:47477/1‑100 (MQ=255)
                                                           gAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGa                                            <  2:4856089/101‑1 (MQ=255)
                                                           gAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  2:2900497/1‑101 (MQ=255)
                                                            aC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:4861498/101‑1 (MQ=255)
                                                             c.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  2:4058032/101‑1 (MQ=255)
                                                               gATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:5295054/101‑1 (MQ=255)
                                                                 tGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  2:9015585/1‑101 (MQ=255)
                                                                  ggccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:2153343/99‑1 (MQ=255)
                                                                  gTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:9054334/101‑1 (MQ=255)
                                                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                    <  2:3593219/101‑1 (MQ=255)
                                                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                    >  1:1972849/1‑101 (MQ=255)
                                                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                    >  2:6324524/1‑101 (MQ=255)
                                                                     cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGt                                   >  1:8855315/1‑101 (MQ=255)
                                                                     cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGt                                   <  2:3456015/101‑1 (MQ=255)
                                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTc                                  >  2:5804981/1‑101 (MQ=255)
                                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                <  2:2219211/101‑1 (MQ=255)
                                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACGTCCa                                >  2:8003097/1‑101 (MQ=255)
                                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGc                              >  2:2443829/1‑101 (MQ=255)
                                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGcc                             >  2:5243986/1‑100 (MQ=255)
                                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                             <  1:1412269/101‑1 (MQ=255)
                                                                            ccccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCCCacgtc                            >  1:455285/1‑97 (MQ=255)
                                                                             cccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTcg                           <  2:7259553/101‑1 (MQ=255)
                                                                                 cgcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGcc                       <  1:8731961/101‑1 (MQ=255)
                                                                                 cgcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGcc                       <  1:760442/101‑1 (MQ=255)
                                                                                  gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                      >  2:2766180/1‑101 (MQ=255)
                                                                                   cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                     <  2:8816369/101‑1 (MQ=255)
                                                                                     ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                   <  1:2659948/101‑1 (MQ=255)
                                                                                         cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt               >  2:2339456/1‑101 (MQ=255)
                                                                                           tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc             <  2:1118744/101‑1 (MQ=255)
                                                                                           tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc             <  1:6909261/101‑1 (MQ=255)
                                                                                            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg            <  2:3752996/101‑1 (MQ=255)
                                                                                               tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc         <  1:2452528/101‑1 (MQ=255)
                                                                                                 gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGt       <  2:3284748/101‑1 (MQ=255)
                                                                                                   aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTgc     <  1:4414966/101‑1 (MQ=255)
                                                                                                   aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTgc     <  1:6494290/101‑1 (MQ=255)
                                                                                                     cTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCCCTCCCCCCGCGGGGGCGTGGATCGTAACGTCCAGCTCGCGCCACATGTCGTCGACCGTgcgc   <  1:6569787/101‑1 (MQ=255)
                                                                                                     cTGCCGGCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCCCCACATGTCTTCCACAGTgcgc   >  2:6216444/1‑101 (MQ=38)
                                                                                                      |                                                                                                    
GTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCC  >  NC_014034/1396966‑1397166

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG