Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,147 C→A 4.3% H343Q (CAC→CAA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1470C→A 4.3% 36.5 ‑1.4 ‑0.2439H343Q (CAC→CAARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/12):  ref base (213/207):  total (220/219)
Fisher's exact test strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.95e-01

Only 100 of 484 total aligned reads displayed.

CGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGC  >  NC_014034/1397047‑1397247
                                                                                                     |                                                                                                     
 gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                                      <  2:6478228/101‑1 (MQ=255)
 gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                                      >  1:180528/1‑101 (MQ=255)
  ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                                     <  1:8399205/101‑1 (MQ=255)
  ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                                     >  1:729774/1‑101 (MQ=255)
   gggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATg                                                                                                    >  1:8667026/1‑101 (MQ=255)
   gggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATg                                                                                                    <  2:1604928/101‑1 (MQ=255)
      cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                                 >  2:2339456/1‑101 (MQ=255)
       gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                                <  2:105036/101‑1 (MQ=255)
        tGGATCGCAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                               <  2:6233310/101‑1 (MQ=255)
        tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                               >  1:7495061/1‑101 (MQ=255)
        tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGGCTTc                                                                                               >  2:1973692/1‑101 (MQ=255)
          gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                                             >  2:1446932/1‑101 (MQ=255)
           aTCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc                                                                                            >  2:302242/1‑101 (MQ=255)
           aTCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc                                                                                            >  2:1346898/1‑101 (MQ=255)
            tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                                           >  1:3462900/1‑101 (MQ=255)
             cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                                         <  1:6556873/101‑1 (MQ=255)
               aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                                       <  2:5777371/101‑1 (MQ=255)
                   tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                                   <  1:7727674/101‑1 (MQ=255)
                   tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                                   <  1:8538856/101‑1 (MQ=255)
                    gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCg                                                                                  >  1:4499126/1‑101 (MQ=255)
                     ccGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGt                                                                                 <  1:1346930/101‑1 (MQ=255)
                       gTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCg                                                                               >  2:558847/1‑101 (MQ=255)
                        tCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGc                                                                              <  2:5010418/101‑1 (MQ=255)
                          ttGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGTGCCGTCGCTc                                                                            >  2:8153964/1‑101 (MQ=255)
                           tGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                                           >  1:5544229/1‑101 (MQ=255)
                            gAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                                          >  1:3068302/1‑101 (MQ=255)
                              gTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                        >  1:630404/1‑101 (MQ=255)
                              gTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                        <  1:6700967/101‑1 (MQ=255)
                              gTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                        <  2:656934/101‑1 (MQ=255)
                               tGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                       >  2:2210889/1‑101 (MQ=255)
                                gATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                      <  1:1223404/101‑1 (MQ=255)
                                gATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                      <  2:7210558/101‑1 (MQ=255)
                                 aTGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgc                                                                     <  1:7145578/101‑1 (MQ=255)
                                   ggggCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                   >  2:4533263/1‑101 (MQ=255)
                                   ggggCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                   >  2:2462409/1‑101 (MQ=255)
                                           aGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:2709797/101‑1 (MQ=255)
                                           aGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                           >  1:1727071/1‑101 (MQ=255)
                                              ggctgcggtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:4213161/94‑1 (MQ=0)
                                              acaTGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:3945153/101‑1 (MQ=255)
                                               gctgcggtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       >  2:3595601/7‑101 (MQ=0)
                                               gctgcggtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  1:6637572/95‑1 (MQ=0)
                                                ctgcggtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      <  1:5975737/96‑1 (MQ=2)
                                                aTGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:1106612/1‑101 (MQ=255)
                                                aTGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:9141579/1‑101 (MQ=255)
                                                 tGTCGTCGCTCCCCCCCGCGGGGGCGGGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GGGCGCCGCCGCTCCCCCCCCGGGGGCGGGGATCGa                                                      >  1:6932666/1‑101 (MQ=37)
                                                 tGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  2:5167294/101‑1 (MQ=255)
                                                 tGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  1:3626170/101‑1 (MQ=255)
                                                  gtcgtcGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  1:2525109/1‑101 (MQ=255)
                                                  gtcgtcGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  1:164079/1‑101 (MQ=255)
                                                     gtcgCT.CCCCCCGCGGGGGCGTGGGTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACgg                                                 >  2:4375449/1‑100 (MQ=255)
                                                     gtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgg                                                 >  1:785791/1‑100 (MQ=255)
                                                      tcgCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                                <  1:5242345/101‑1 (MQ=255)
                                                        gCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                              >  1:3825268/1‑101 (MQ=255)
                                                        gCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                              >  2:4745415/1‑101 (MQ=255)
                                                         cT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                             >  1:5347348/1‑101 (MQ=255)
                                                          t.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                                            <  2:3246446/101‑1 (MQ=255)
                                                          t.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                                            >  1:1463999/1‑101 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                           >  1:5665970/1‑101 (MQ=255)
                                                             cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                                          <  1:3591340/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                                       <  1:2163319/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                                       >  2:1794073/1‑101 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                                       <  2:831360/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacg                                       <  2:4142086/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCaag                                       <  1:1942019/101‑3 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacga                                      >  1:4004451/1‑101 (MQ=255)
                                                                  gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                                     >  2:8915637/1‑101 (MQ=255)
                                                                  gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                                     <  1:5897263/101‑1 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                                    >  2:2808579/1‑101 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                                    <  1:7431001/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGc                                   >  2:6700341/1‑101 (MQ=255)
                                                                       ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCt                                <  2:7418958/101‑1 (MQ=255)
                                                                        gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                               <  1:2994366/101‑1 (MQ=255)
                                                                         ggTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGc                              <  1:8963442/100‑1 (MQ=255)
                                                                           tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACGGGTCTGCCACCACGCCCTGccc                            >  2:8790886/1‑99 (MQ=255)
                                                                            ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc                           >  2:7907358/1‑101 (MQ=255)
                                                                             gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa                          >  1:2406443/1‑101 (MQ=255)
                                                                             gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa                          <  1:8310250/101‑1 (MQ=255)
                                                                              gtCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg                         <  2:7751328/100‑1 (MQ=255)
                                                                              aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAg                         <  1:5805216/101‑1 (MQ=255)
                                                                               tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa                        <  1:4983960/101‑1 (MQ=255)
                                                                                 gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc                      >  2:8688348/1‑101 (MQ=255)
                                                                                 gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc                      >  2:7629314/1‑101 (MQ=255)
                                                                                  aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc                     >  1:6487641/1‑101 (MQ=255)
                                                                                  aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc                     >  2:6137166/1‑101 (MQ=255)
                                                                                   aaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcg                    <  1:1022614/101‑1 (MQ=255)
                                                                                    aCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgc                   <  2:2551059/101‑1 (MQ=255)
                                                                                     cGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgcc                  >  2:5282492/1‑100 (MQ=255)
                                                                                      gTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCgcgc                 >  2:633226/1‑101 (MQ=255)
                                                                                      gTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCgcgc                 >  1:2785630/1‑101 (MQ=255)
                                                                                       tCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGcc                >  2:3794725/1‑101 (MQ=255)
                                                                                        ccAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg               <  2:6932381/101‑1 (MQ=255)
                                                                                        ccAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCg               <  1:8087313/101‑1 (MQ=255)
                                                                                         cAGCTCGCGCCGCATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGt              <  2:3392662/101‑1 (MQ=255)
                                                                                          aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTCCACCAGATCCGCGCCGTc             >  1:4717219/1‑101 (MQ=255)
                                                                                            cTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGc           >  1:6153364/1‑101 (MQ=255)
                                                                                            cTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGc           >  2:8390667/1‑101 (MQ=255)
                                                                                             tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCt          >  2:6861750/1‑101 (MQ=255)
                                                                                               gcgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTcc        >  1:5004771/1‑101 (MQ=255)
                                                                                                 gcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTcccc      <  2:1148622/101‑1 (MQ=255)
                                                                                                    acaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcg   <  1:3300705/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
CGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGC  >  NC_014034/1397047‑1397247

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG