Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,162 G→C 4.8% V348V (GTG→GCC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1620G→C 4.8% 33.6 ‑1.4 ‑0.2393V348V (GTG→GTCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/12):  ref base (192/182):  total (199/194)
Fisher's exact test strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.74e-01

Only 100 of 441 total aligned reads displayed.

AAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397062‑1397262
                                                                                                      |                                                                                                     
 aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgc                                                                                                      <  2:8515976/101‑1 (MQ=255)
   cTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCCCT.CCCCCCGCGGGGGCGTGGATCGTAACGTCCAGCTCGCGCCACATGTCGTCGACC.GTgcgc                                                                                                    <  1:6569787/101‑1 (MQ=255)
    tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                                                   <  1:1769472/101‑1 (MQ=255)
    tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                                                   <  1:8538856/101‑1 (MQ=255)
    tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                                                   <  1:7727674/101‑1 (MQ=255)
      ccgatctGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGt                                                                                                 <  2:2585190/95‑1 (MQ=255)
           ttGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                                                            >  1:4810341/1‑101 (MQ=255)
            tGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                                                           <  2:6311492/101‑1 (MQ=255)
            tGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                                                           <  1:259042/101‑1 (MQ=255)
             gAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                                                          <  1:3609724/101‑1 (MQ=255)
              aGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                                                         <  2:575807/101‑1 (MQ=255)
               gTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                                        >  1:630404/1‑101 (MQ=255)
               gTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                                        <  1:6700967/101‑1 (MQ=255)
               gTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                                        <  1:4062277/101‑1 (MQ=255)
                 gATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                      >  1:6124142/1‑101 (MQ=255)
                 gATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                      >  1:3392766/1‑101 (MQ=255)
                 gATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                      <  1:1223404/101‑1 (MQ=255)
                  aTGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgc                                                                                     <  1:7145578/101‑1 (MQ=255)
                  aTGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgc                                                                                     >  1:815935/1‑101 (MQ=255)
                  aTGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgc                                                                                     >  2:6548619/1‑101 (MQ=255)
                    ggggCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                                   >  2:2462409/1‑101 (MQ=255)
                    ggggCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                                   >  2:4533263/1‑101 (MQ=255)
                      ggCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgggg                                                                                 >  1:2066808/1‑101 (MQ=255)
                      ggCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGACGCTCCCCCCGCgggg                                                                                 >  1:7536435/1‑101 (MQ=255)
                       gcgcGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCggggg                                                                                <  1:5260952/101‑1 (MQ=255)
                          cgAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGt                                                                             <  2:1848390/101‑1 (MQ=255)
                           gAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                            >  2:1686376/1‑101 (MQ=255)
                            aGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                           <  2:4123348/101‑1 (MQ=255)
                            aGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                           <  2:3945475/101‑1 (MQ=255)
                              cacaTGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         <  2:5646356/101‑1 (MQ=255)
                               ggctgcggtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  1:3567531/94‑1 (MQ=0)
                               acaTGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        >  1:8478819/1‑101 (MQ=255)
                               acaAGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTGCCCCCGCGGGGGCGTGGATc                                                                        >  1:8812446/1‑101 (MQ=255)
                                gctgcggtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       >  2:3595601/7‑101 (MQ=0)
                                caTGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  1:8330450/101‑1 (MQ=255)
                                 ctgcggtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                      <  1:5975737/96‑1 (MQ=2)
                                 aTGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                      >  2:8820309/1‑101 (MQ=255)
                                 aTGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                      >  1:9141579/1‑101 (MQ=255)
                                  tGTCGTCGCTCCCCCCCGCGGGGGCGGGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GGGCGCCGCCGCTCCCCCCCCGGGGGCGGGGATCGa                                                                      >  1:6932666/1‑101 (MQ=37)
                                   gtcgtcGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                    >  1:164079/1‑101 (MQ=255)
                                   gcggtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                    <  2:7044102/98‑1 (MQ=2)
                                    tcgtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   <  1:2470071/101‑1 (MQ=255)
                                    cggtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   >  1:6640275/3‑101 (MQ=2)
                                     cgtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAGACg                                                                  >  1:8053316/1‑101 (MQ=255)
                                     cgtcgCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                  >  1:7311597/1‑101 (MQ=255)
                                      gtcgCT.CCCCCCGCGGGGGCGTGGGTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACgg                                                                 >  2:4375449/1‑100 (MQ=255)
                                        cgCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                                               <  1:2907073/101‑1 (MQ=255)
                                         gCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                              <  1:1446979/101‑1 (MQ=255)
                                         gCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                              >  2:4745415/1‑101 (MQ=255)
                                         gCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTc                                                              >  1:1119094/1‑101 (MQ=255)
                                          cT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                             <  1:3067443/101‑1 (MQ=255)
                                          cT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCACCCGCGGGGGCGTGGATCGAAACGTGTCt                                                             <  1:1720393/101‑1 (MQ=255)
                                          cT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                             <  2:8052976/101‑1 (MQ=255)
                                             ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                                           >  1:1479171/1‑101 (MQ=255)
                                               ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                                         <  2:8163087/101‑1 (MQ=255)
                                                cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.c                                                       <  2:2164414/101‑1 (MQ=255)
                                                 ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cg                                                      <  1:5098346/101‑1 (MQ=255)
                                                 ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cg                                                      <  1:4533398/101‑1 (MQ=255)
                                                    cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgacg                                                   <  1:7431001/101‑1 (MQ=255)
                                                     gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGc                                                  <  1:587911/101‑1 (MQ=255)
                                                     gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGc                                                  >  2:6700341/1‑101 (MQ=255)
                                                       gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGccc                                                <  2:3052671/101‑1 (MQ=255)
                                                        ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                               <  2:7418958/101‑1 (MQ=255)
                                                           gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCa                                            <  2:5949264/101‑1 (MQ=255)
                                                           gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCa                                            >  2:6431881/1‑101 (MQ=255)
                                                           gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCa                                            >  1:5716131/1‑101 (MQ=255)
                                                              gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCa                                         <  2:6176458/101‑1 (MQ=255)
                                                              gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCa                                         <  1:7830912/101‑1 (MQ=255)
                                                               gtCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAg                                        <  2:3249640/100‑1 (MQ=255)
                                                               gtCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAg                                        <  2:7751328/100‑1 (MQ=255)
                                                                 cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCTGCCA.CGCCGCCCTGCACCAGac                                      >  1:2375067/1‑100 (MQ=255)
                                                                  gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATc                                     <  2:8734370/101‑1 (MQ=255)
                                                                  gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATc                                     >  2:7629314/1‑101 (MQ=255)
                                                                   aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                    >  2:2319754/1‑101 (MQ=255)
                                                                   aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                    >  1:6487641/1‑101 (MQ=255)
                                                                    aaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                   <  1:1022614/101‑1 (MQ=255)
                                                                        tCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGcc                               >  2:3794725/1‑101 (MQ=255)
                                                                         ccAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCg                              <  2:5187919/101‑1 (MQ=255)
                                                                           aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCATGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                            >  1:4986309/1‑101 (MQ=255)
                                                                             ctggcgcCACATGGCTTCTGCC.GTCCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGc                          <  2:5436539/98‑1 (MQ=37)
                                                                              tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCt                         <  1:2751183/101‑1 (MQ=255)
                                                                              tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCt                         >  2:6861750/1‑101 (MQ=255)
                                                                                  gcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccc                     <  2:1148622/101‑1 (MQ=255)
                                                                                  gcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccc                     <  1:5459569/101‑1 (MQ=255)
                                                                                    cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                   >  2:5067835/1‑101 (MQ=255)
                                                                                    cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                   <  2:1462619/101‑1 (MQ=255)
                                                                                    cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                   <  1:3714183/101‑1 (MQ=255)
                                                                                     acaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccccccc                  >  1:299999/1‑100 (MQ=255)
                                                                                      caTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATGCGCGCCGTCGCTCCCCCcgc                 >  1:5613923/1‑101 (MQ=255)
                                                                                      caTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCCCCCCCcgc                 >  2:1057850/1‑101 (MQ=255)
                                                                                         gTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGAAGCCCTGCACCAGATCGAGGACGGCGCTCCCGCCACggg              >  2:8933063/1‑101 (MQ=25)
                                                                                              cGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGt         <  1:9016007/101‑1 (MQ=255)
                                                                                               gACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg        >  2:6631608/1‑101 (MQ=255)
                                                                                               gACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg        >  2:1288169/1‑101 (MQ=255)
                                                                                                    gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc    >  2:2812547/1‑101 (MQ=255)
                                                                                                    gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc    >  2:3361429/1‑101 (MQ=255)
                                                                                                    gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCAGCGCCGTCGCTCCCCCCGCGGGGGAGTGGATc    >  2:8324677/1‑101 (MQ=255)
                                                                                                      gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCg   <  1:4308337/101‑1 (MQ=2)
                                                                                                      gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCg   <  1:3240430/101‑1 (MQ=2)
                                                                                                      gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa  <  1:1973752/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
AAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397062‑1397262

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG