Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,201 G→T 1.9% V361V (GTG→GCT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2010G→T 1.9% 8.0 ‑1.8 ‑0.7408V361V (GTG→GTTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (204/194):  total (206/202)
Fisher's exact test strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.82e-01

Only 100 of 438 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACG  >  NC_014034/1397101‑1397301
                                                                                                    |                                                                                                       
tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                                                                                         <  1:5242345/101‑1 (MQ=255)
tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                                                                                         >  1:3922479/1‑101 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.c                                                                                                      >  1:1119094/1‑101 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.c                                                                                                      >  1:7916135/1‑101 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.Ct                                                                                                     >  2:7773054/1‑101 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.Ct                                                                                                     <  1:16328/101‑1 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.Ct                                                                                                     <  1:5922016/101‑1 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGTGT.Ct                                                                                                     <  2:8052976/101‑1 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTg                                                                                                    <  1:6647658/101‑1 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGc                                                                                                   >  2:383122/1‑101 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGc                                                                                                   <  2:8367238/101‑1 (MQ=255)
        cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.c                                                                                               <  1:9053380/101‑1 (MQ=255)
         ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cg                                                                                              <  1:8873344/101‑1 (MQ=255)
           gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cgac                                                                                            <  2:8785751/101‑1 (MQ=255)
           gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cgac                                                                                            <  2:1438019/101‑1 (MQ=255)
            cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cgacg                                                                                           >  2:2808579/1‑101 (MQ=255)
              ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGcc                                                                                         <  2:2246970/101‑1 (MQ=255)
              ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGT.CTGCCA.CGACGcc                                                                                         >  2:4727847/1‑101 (MQ=255)
               gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGccc                                                                                        <  2:6438737/101‑1 (MQ=255)
               gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGccc                                                                                        <  2:4463131/101‑1 (MQ=255)
                ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCt                                                                                       >  1:5461253/1‑101 (MQ=255)
                 gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTg                                                                                      <  2:8639853/101‑1 (MQ=255)
                    tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAc                                                                                   <  2:6878335/101‑1 (MQ=255)
                     ggATCGAAACGTTCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAcc                                                                                  >  1:6534122/1‑101 (MQ=255)
                     ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAcc                                                                                  >  2:7907358/1‑101 (MQ=255)
                     ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAcc                                                                                  >  1:8519518/1‑101 (MQ=255)
                      gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCa                                                                                 <  1:8310250/101‑1 (MQ=255)
                      gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCa                                                                                 >  1:2406443/1‑101 (MQ=255)
                       gtCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAg                                                                                <  2:3249640/100‑1 (MQ=255)
                       aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTTGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCC.CGACGCCCTGCACCAg                                                                                >  2:7280733/1‑101 (MQ=255)
                       aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAg                                                                                <  1:5805216/101‑1 (MQ=255)
                           aaaCGGCCCGCTCGCTCCACATTTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATcc                                                                            <  2:2513854/101‑1 (MQ=37)
                            aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                           <  1:1022614/101‑1 (MQ=255)
                            aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                           <  2:8889504/101‑1 (MQ=255)
                             aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgc                                                                          <  2:2551059/101‑1 (MQ=255)
                             aCGTCCAGCCCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgc                                                                          <  1:2928004/101‑1 (MQ=255)
                              cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                         >  1:1730559/1‑101 (MQ=255)
                              cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                         >  2:8690513/1‑101 (MQ=255)
                              cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcc                                                                         >  2:5282492/1‑100 (MQ=255)
                               gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCgcgc                                                                        >  2:633226/1‑101 (MQ=255)
                                tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                       >  1:1299490/1‑101 (MQ=255)
                                   aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCATGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTc                                                                   >  1:4986309/1‑101 (MQ=255)
                                      tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCt                                                                <  1:2751183/101‑1 (MQ=255)
                                      tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCt                                                                <  1:7139506/101‑1 (MQ=255)
                                           ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                           <  2:1744325/101‑1 (MQ=255)
                                            cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccccc                                                          >  1:7442715/1‑101 (MQ=255)
                                             acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcg                                                         <  1:3300705/101‑1 (MQ=255)
                                                  tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAGACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgggg                                                    >  2:6259679/1‑101 (MQ=255)
                                                    ttCGACCGTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGATCCCCCCGCGGGGGc                                                  >  2:6696957/1‑101 (MQ=255)
                                                     tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                 >  2:3908683/1‑101 (MQ=255)
                                                      cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                >  2:5752823/1‑100 (MQ=255)
                                                      cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                >  1:2214901/1‑100 (MQ=255)
                                                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           >  1:3066065/1‑101 (MQ=255)
                                                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           >  2:3361429/1‑101 (MQ=255)
                                                           cttcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  2:6595853/98‑1 (MQ=255)
                                                             gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  1:4308337/101‑1 (MQ=2)
                                                             gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         <  1:1973752/101‑1 (MQ=255)
                                                               ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  2:1224887/99‑1 (MQ=2)
                                                               gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  1:1823574/1‑101 (MQ=255)
                                                               gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:3043191/101‑1 (MQ=255)
                                                               gcCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  2:8206163/101‑1 (MQ=255)
                                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                     >  1:5699109/1‑101 (MQ=255)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                   >  1:4056741/1‑101 (MQ=255)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                   <  1:7822697/101‑1 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                  >  2:2366669/1‑101 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                  <  1:2623190/101‑1 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                  <  1:6137403/101‑1 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                <  1:8688753/101‑1 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                               >  1:4701392/1‑101 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                               >  1:288452/1‑101 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                             <  2:6153127/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCCcg                            >  2:5058480/1‑101 (MQ=255)
                                                                           cccGCGGGGGCGTGGATCGAAACCTATGAGGCCT.GGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacatcagcttga                            <  2:7539059/101‑10 (MQ=2)
                                                                             cgcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                         <  2:1890764/101‑1 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                       <  1:4396070/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                      >  2:4945525/1‑101 (MQ=255)
                                                                                 ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                     <  1:7377340/101‑1 (MQ=255)
                                                                                  gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                    >  1:3581950/1‑101 (MQ=255)
                                                                                  gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                    <  2:4113956/101‑1 (MQ=255)
                                                                                  gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                    >  1:3808433/1‑101 (MQ=255)
                                                                                   ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGCACGCGCGCACgg                   >  1:3218882/1‑101 (MQ=255)
                                                                                    gCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGc                  >  2:1144593/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                >  2:6634966/1‑101 (MQ=255)
                                                                                        ggatgaaaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCGCGCACGCGCGAACGGCGTCa              <  2:299996/95‑1 (MQ=255)
                                                                                        ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa              <  2:5482176/101‑1 (MQ=255)
                                                                                        ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa              >  1:6390869/1‑101 (MQ=255)
                                                                                            cGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc          >  2:3630567/1‑101 (MQ=255)
                                                                                             gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc         <  2:7647234/101‑1 (MQ=255)
                                                                                             gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc         >  1:9198797/1‑101 (MQ=255)
                                                                                             gAAACGCGT.CGGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACTGCGCACGCGCGAACGGCGCCAGcccc         >  2:5914714/1‑101 (MQ=37)
                                                                                              aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg        >  2:9094745/1‑101 (MQ=255)
                                                                                              aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg        <  1:475670/101‑1 (MQ=255)
                                                                                              aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg        >  2:1494724/1‑101 (MQ=255)
                                                                                              aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg        >  2:3730137/1‑101 (MQ=255)
                                                                                                aCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg      <  1:8137083/101‑1 (MQ=255)
                                                                                                aCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg      <  1:3397606/101‑1 (MQ=255)
                                                                                                 cGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGa     <  2:3077080/101‑1 (MQ=255)
                                                                                                  gtgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGGGTCAGCCCCGCGaa    >  2:4246329/1‑101 (MQ=255)
                                                                                                   tgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc   <  1:5068004/101‑1 (MQ=255)
                                                                                                   tgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCCAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc   <  1:6862022/101‑1 (MQ=255)
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TCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACG  >  NC_014034/1397101‑1397301

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG