Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,202 T→G 2.0% S362A (TCT→GCT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2020T→G 2.0% 10.2 ‑1.8 ‑1.0408S362A (TCT→GCT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (204/196):  total (206/202)
Fisher's exact test strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.20e-02

Only 100 of 438 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGT  >  NC_014034/1397102‑1397302
                                                                                                    |                                                                                                       
 gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.c                                                                                                       >  1:1119094/1‑101 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.Ct                                                                                                      <  1:16328/101‑1 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.Ct                                                                                                      >  2:7773054/1‑101 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGTGT.Ct                                                                                                      <  2:8052976/101‑1 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGc                                                                                                    <  2:8367238/101‑1 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGc                                                                                                    >  2:383122/1‑101 (MQ=255)
      ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa                                                                                                  <  1:7145359/101‑1 (MQ=255)
      ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa                                                                                                  <  1:5307823/101‑1 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.c                                                                                                <  1:9053380/101‑1 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.c                                                                                                <  1:6864101/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cg                                                                                               <  1:8873344/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cg                                                                                               <  1:5098346/101‑1 (MQ=255)
         cgcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cga                                                                                              >  1:4004451/1‑101 (MQ=255)
          gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cgac                                                                                             <  2:1438019/101‑1 (MQ=255)
          gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cgac                                                                                             <  2:8785751/101‑1 (MQ=255)
           cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cgacg                                                                                            >  2:2808579/1‑101 (MQ=255)
            gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGc                                                                                           >  1:6066649/1‑101 (MQ=255)
            gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGc                                                                                           <  1:587911/101‑1 (MQ=255)
            gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGc                                                                                           >  1:499110/1‑101 (MQ=255)
             ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGcc                                                                                          <  2:2246970/101‑1 (MQ=255)
             ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGT.CTGCCA.CGACGcc                                                                                          >  2:4727847/1‑101 (MQ=255)
              gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGccc                                                                                         <  2:6438737/101‑1 (MQ=255)
              gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGccc                                                                                         <  2:4463131/101‑1 (MQ=255)
                gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTg                                                                                       <  2:8639853/101‑1 (MQ=255)
                   tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAc                                                                                    <  2:6878335/101‑1 (MQ=255)
                    ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAcc                                                                                   >  1:8519518/1‑101 (MQ=255)
                    ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAcc                                                                                   >  2:7907358/1‑101 (MQ=255)
                    ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAcc                                                                                   >  1:6213211/1‑101 (MQ=255)
                     gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCa                                                                                  <  1:7830912/101‑1 (MQ=255)
                     gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCa                                                                                  >  1:2406443/1‑101 (MQ=255)
                      gtCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAg                                                                                 <  2:3249640/100‑1 (MQ=255)
                      aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTTGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCC.CGACGCCCTGCACCAg                                                                                 >  2:7280733/1‑101 (MQ=255)
                          aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATcc                                                                             <  1:7405313/101‑1 (MQ=255)
                          aaaCGGCCCGCTCGCTCCACATTTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATcc                                                                             <  2:2513854/101‑1 (MQ=37)
                           aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                            >  1:5749385/1‑101 (MQ=255)
                           aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                            <  2:8889504/101‑1 (MQ=255)
                           aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                            <  1:1022614/101‑1 (MQ=255)
                            aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgc                                                                           <  2:2551059/101‑1 (MQ=255)
                            aCGTCCAGCCCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgc                                                                           <  1:2928004/101‑1 (MQ=255)
                             cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                          >  2:8690513/1‑101 (MQ=255)
                             cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                          >  1:1730559/1‑101 (MQ=255)
                             cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcc                                                                          >  2:5282492/1‑100 (MQ=255)
                              gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCgcgc                                                                         >  2:633226/1‑101 (MQ=255)
                               tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                        >  1:1299490/1‑101 (MQ=255)
                                ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                      >  1:4493751/1‑101 (MQ=255)
                                  aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTCCACCAGATCCGCGCC.GTc                                                                    >  1:4717219/1‑101 (MQ=255)
                                     tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCt                                                                 <  1:2751183/101‑1 (MQ=255)
                                          ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                            <  2:1744325/101‑1 (MQ=255)
                                            acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcg                                                          <  1:3300705/101‑1 (MQ=255)
                                                 tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAGACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgggg                                                     >  2:6259679/1‑101 (MQ=255)
                                                   ttCGACCGTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGATCCCCCCGCGGGGGc                                                   >  2:6696957/1‑101 (MQ=255)
                                                    tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                  >  2:3908683/1‑101 (MQ=255)
                                                     cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                 >  2:5752823/1‑100 (MQ=255)
                                                     cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                 >  1:2214901/1‑100 (MQ=255)
                                                     cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCGGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                 >  1:6558027/1‑100 (MQ=255)
                                                      gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                >  1:8140085/1‑101 (MQ=255)
                                                         cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  1:40738/1‑101 (MQ=255)
                                                          gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  1:3066065/1‑101 (MQ=255)
                                                          gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  2:3361429/1‑101 (MQ=255)
                                                          cttcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:6595853/98‑1 (MQ=255)
                                                            gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  1:1973752/101‑1 (MQ=255)
                                                             cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:4370970/101‑1 (MQ=255)
                                                              ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  2:1224887/99‑1 (MQ=2)
                                                              gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  1:3043191/101‑1 (MQ=255)
                                                              gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  1:1823574/1‑101 (MQ=255)
                                                              gcCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  2:8206163/101‑1 (MQ=255)
                                                               ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                       <  1:6414000/101‑1 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                   <  1:2623190/101‑1 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                   >  2:2366669/1‑101 (MQ=255)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                 <  1:8688753/101‑1 (MQ=255)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                                >  1:288452/1‑101 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                              <  1:745814/101‑1 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                              <  2:6153127/101‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCCcg                             >  2:5058480/1‑101 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACCTATGAGGCCT.GGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacatcagcttga                             <  2:7539059/101‑10 (MQ=2)
                                                                            cgcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                          <  2:1890764/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                       >  2:4945525/1‑101 (MQ=255)
                                                                                 gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                     <  2:4113956/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                     >  1:3581950/1‑101 (MQ=255)
                                                                                 gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                     >  1:3808433/1‑101 (MQ=255)
                                                                                  ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGCACGCGCGCACgg                    >  1:3218882/1‑101 (MQ=255)
                                                                                   gCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGc                   >  2:1144593/1‑101 (MQ=255)
                                                                                     gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                 >  2:6634966/1‑101 (MQ=255)
                                                                                      tGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCTCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                >  1:4834733/1‑101 (MQ=255)
                                                                                       ggatgaaaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCGCGCACGCGCGAACGGCGTCa               <  2:299996/95‑1 (MQ=255)
                                                                                       ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa               <  2:5482176/101‑1 (MQ=255)
                                                                                       ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa               >  1:5665655/1‑101 (MQ=255)
                                                                                       ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa               >  1:7940272/1‑101 (MQ=255)
                                                                                       ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa               >  1:8318774/1‑101 (MQ=255)
                                                                                           cGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc           >  2:3630567/1‑101 (MQ=255)
                                                                                            gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc          <  2:7647234/101‑1 (MQ=255)
                                                                                            gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc          >  1:9198797/1‑101 (MQ=255)
                                                                                            gAAACGCGT.CGGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACTGCGCACGCGCGAACGGCGCCAGcccc          >  2:5914714/1‑101 (MQ=37)
                                                                                             aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg         >  2:9094745/1‑101 (MQ=255)
                                                                                             aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg         >  2:1494724/1‑101 (MQ=255)
                                                                                             aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg         >  2:3730137/1‑101 (MQ=255)
                                                                                               aCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg       <  1:3397606/101‑1 (MQ=255)
                                                                                                cGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGa      <  2:3077080/101‑1 (MQ=255)
                                                                                                 gtgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGGGTCAGCCCCGCGaa     >  2:4246329/1‑101 (MQ=255)
                                                                                                   gt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg   >  1:6661132/1‑101 (MQ=255)
                                                                                                    |                                                                                                       
CGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGT  >  NC_014034/1397102‑1397302

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG