Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,211 A→C 2.6% T365P (ACG→CCA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2110A→C 2.6% 12.7 ‑2.1 ‑0.3385T365P (ACG→CCG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/8):  ref base (203/171):  total (206/179)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.56e-01

Only 100 of 429 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCC  >  NC_014034/1397111‑1397311
                                                                                                      |                                                                                                     
cgcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cga                                                                                                       >  1:2414933/1‑101 (MQ=255)
 gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cgac                                                                                                      >  2:8915637/1‑101 (MQ=255)
  cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCa.cgacg                                                                                                     <  1:7431001/101‑1 (MQ=255)
   gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGc                                                                                                    >  2:6700341/1‑101 (MQ=255)
   gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGc                                                                                                    <  1:587911/101‑1 (MQ=255)
   gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGc                                                                                                    >  1:6066649/1‑101 (MQ=255)
    ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGcc                                                                                                   <  2:8107493/101‑1 (MQ=255)
    ggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGcc                                                                                                   >  2:3668943/1‑101 (MQ=255)
    ggggCGTGGATCGAAACGTCCAGCGCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGcc                                                                                                   <  2:3397789/101‑1 (MQ=255)
     gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGccc                                                                                                  <  1:1104229/101‑1 (MQ=255)
       gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTg                                                                                                <  2:8639853/101‑1 (MQ=255)
         gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCa                                                                                              <  2:5949264/101‑1 (MQ=255)
         gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCa                                                                                              <  1:4605265/101‑1 (MQ=255)
          tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACGGGT.CTGCCA.CCACGCCCTGccc                                                                                             >  2:8790886/1‑99 (MQ=255)
           ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAcc                                                                                            >  2:7907358/1‑101 (MQ=255)
           ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAcc                                                                                            >  2:8234557/1‑101 (MQ=255)
           ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAcc                                                                                            >  1:6213211/1‑101 (MQ=255)
             gtCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAg                                                                                          <  2:3249640/100‑1 (MQ=255)
             aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTTGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCC.CGACGCCCTGCACCAg                                                                                          >  2:7280733/1‑101 (MQ=255)
               cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGAt                                                                                        <  1:8538519/101‑1 (MQ=255)
                  aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                                     <  1:1022614/101‑1 (MQ=255)
                  aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                                     <  1:6728520/101‑1 (MQ=255)
                    cGTCCAGCTCTCGCCACATGTCTTCGCCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                   <  2:3068203/101‑1 (MQ=255)
                    cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                   >  1:1730559/1‑101 (MQ=255)
                    cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                   >  2:8690513/1‑101 (MQ=255)
                    cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACACGATCcgcg                                                                                   >  2:3834066/1‑101 (MQ=255)
                      tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                 >  1:1299490/1‑101 (MQ=255)
                      tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                 >  2:3794725/1‑101 (MQ=255)
                      tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                 <  2:2224563/101‑1 (MQ=255)
                       ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                               <  2:6932381/101‑1 (MQ=255)
                       ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                               <  1:8087313/101‑1 (MQ=255)
                          gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCg                                                                            <  2:1259294/101‑1 (MQ=255)
                           cTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                           >  2:8390667/1‑101 (MQ=255)
                              gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcc                                                                        >  1:5004771/1‑101 (MQ=255)
                               cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                       <  2:4493616/101‑1 (MQ=255)
                                gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                      <  1:5459569/101‑1 (MQ=255)
                                gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                      <  2:1148622/101‑1 (MQ=255)
                                   acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccccc                                                                   >  1:299999/1‑100 (MQ=255)
                                   acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcg                                                                   <  1:3300705/101‑1 (MQ=255)
                                   acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcg                                                                   <  2:3341444/101‑1 (MQ=255)
                                    caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgc                                                                  >  1:4954317/1‑101 (MQ=255)
                                    caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCCCCCCCcgc                                                                  >  2:1057850/1‑101 (MQ=255)
                                         cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggggg                                                             >  2:2801789/1‑101 (MQ=255)
                                           tCGACCGTGCGCCGTCGCTCACCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                           >  1:8223162/1‑101 (MQ=255)
                                            cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCGGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                          >  1:6558027/1‑100 (MQ=255)
                                             gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                         <  1:7689737/101‑1 (MQ=255)
                                                 gTGCGCCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  2:66314/101‑1 (MQ=255)
                                                   gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:4039281/1‑101 (MQ=2)
                                                   gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  1:1973752/101‑1 (MQ=255)
                                                    cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  1:3061887/101‑1 (MQ=255)
                                                    cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:6336835/101‑1 (MQ=255)
                                                    cgcCGTCGCTCCCCCCGCGGGGGCATGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:4986149/101‑1 (MQ=255)
                                                     gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  1:1823574/1‑101 (MQ=255)
                                                      ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                <  1:6414000/101‑1 (MQ=255)
                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                               >  1:5699109/1‑101 (MQ=255)
                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                               <  2:2550154/101‑1 (MQ=255)
                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGCGGCGTGGATAGACacac                                              >  2:2332267/1‑101 (MQ=255)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                           >  2:4158980/1‑101 (MQ=255)
                                                            caccccccGCGGGGGCGTGGATCGAAACCTGT.CTGCCG.CGACGCCGTGCGCCAGATCTGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                          <  2:5734566/99‑1 (MQ=25)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                          <  1:8688753/101‑1 (MQ=255)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                                         >  1:412004/1‑101 (MQ=255)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                                         >  1:288452/1‑101 (MQ=255)
                                                             cccccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                                         <  1:8908717/100‑1 (MQ=255)
                                                               cccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                       <  2:6153127/101‑1 (MQ=255)
                                                                 cccGCGGGGGCGTGGATCGAAACCTATGAGGCCT.GGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacatcagcttga                                      <  2:7539059/101‑10 (MQ=2)
                                                                   cgcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                   <  2:6260234/101‑1 (MQ=255)
                                                                   cgcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                   <  1:4727996/101‑1 (MQ=255)
                                                                   cgcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                   <  2:1890764/101‑1 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGNAACACCGCGCAcgcgcg                                  >  2:2898527/1‑101 (MQ=255)
                                                                      gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                >  1:5113323/1‑101 (MQ=255)
                                                                      gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                >  1:7905846/1‑101 (MQ=255)
                                                                      gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                >  1:2758334/1‑101 (MQ=255)
                                                                       ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                               <  1:7377340/101‑1 (MQ=255)
                                                                       ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                               <  2:510115/101‑1 (MQ=255)
                                                                        gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                              >  1:3581950/1‑101 (MQ=255)
                                                                        gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                              >  1:3808433/1‑101 (MQ=255)
                                                                         ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                             <  2:1457448/101‑1 (MQ=255)
                                                                         ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGCACGCGCGCACgg                             >  1:3218882/1‑101 (MQ=255)
                                                                          gCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGc                            <  1:6937301/101‑1 (MQ=255)
                                                                          gCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGc                            <  2:7789752/101‑1 (MQ=255)
                                                                           cGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                           >  2:5804636/1‑101 (MQ=255)
                                                                            gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGCACGGCgc                          >  2:4834036/1‑100 (MQ=255)
                                                                            gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                          >  2:5319261/1‑101 (MQ=255)
                                                                            gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                          >  1:4010162/1‑101 (MQ=255)
                                                                                aTCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                      >  2:9137209/1‑101 (MQ=255)
                                                                                aTCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                      >  2:2379654/1‑101 (MQ=255)
                                                                                aTCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                      <  2:1696390/101‑1 (MQ=255)
                                                                                   gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                   <  2:6521826/101‑1 (MQ=255)
                                                                                   gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                   <  2:7647234/101‑1 (MQ=255)
                                                                                   gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                   >  1:9190964/1‑101 (MQ=255)
                                                                                    aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCCGCCCcg                  >  2:5462562/1‑101 (MQ=255)
                                                                                    aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                  >  2:1494724/1‑101 (MQ=255)
                                                                                      aCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                <  1:3397606/101‑1 (MQ=255)
                                                                                        gtgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa              >  1:223600/1‑101 (MQ=255)
                                                                                         tgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc             <  1:437174/101‑1 (MQ=255)
                                                                                             cTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc          <  2:47477/101‑1 (MQ=255)
                                                                                              tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg         >  1:2689786/1‑101 (MQ=255)
                                                                                               gCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc        <  2:4354110/101‑1 (MQ=255)
                                                                                                      acgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc  <  2:6660872/101‑1 (MQ=255)
                                                                                                      acgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc  <  1:8370823/101‑1 (MQ=255)
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CGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCC  >  NC_014034/1397111‑1397311

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG