Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,217 T→A 2.0% C367S (TGC→AAT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2170T→A 2.0% 12.2 ‑1.9 ‑0.2404C367S (TGC→AGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (214/179):  total (217/187)
Fisher's exact test strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.95e-01

Only 100 of 421 total aligned reads displayed.

GGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGG  >  NC_014034/1397117‑1397317
                                                                                                     |                                                                                                     
    tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACGGGTCT.GCCACCACGCCCTGccc                                                                                                   >  2:8790886/1‑99 (MQ=255)
    tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCAc                                                                                                   <  2:4059204/101‑1 (MQ=255)
     ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCAcc                                                                                                  >  2:8234557/1‑101 (MQ=255)
      gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCa                                                                                                 <  1:7830912/101‑1 (MQ=255)
      gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCa                                                                                                 >  1:2406443/1‑101 (MQ=255)
      gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCa                                                                                                 <  2:6176458/101‑1 (MQ=255)
       aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAg                                                                                                <  1:6216683/101‑1 (MQ=255)
        tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGa                                                                                               <  1:4983960/101‑1 (MQ=255)
         cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGAt                                                                                              >  1:8200762/1‑101 (MQ=255)
         cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGAt                                                                                              <  2:481825/101‑1 (MQ=255)
          gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATc                                                                                             >  2:6300530/1‑101 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATcc                                                                                            <  1:8367394/101‑1 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATcc                                                                                            <  2:3721314/101‑1 (MQ=255)
           aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATcc                                                                                            >  2:4395944/1‑101 (MQ=255)
            aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCcg                                                                                           <  2:7712889/101‑1 (MQ=255)
            aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCcg                                                                                           <  1:1022614/101‑1 (MQ=255)
             aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCcgc                                                                                          >  1:6428487/1‑101 (MQ=255)
              cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCcgcg                                                                                         >  1:8912603/1‑101 (MQ=255)
              cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCcgcg                                                                                         >  2:8690513/1‑101 (MQ=255)
              cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCcgcc                                                                                         >  2:5282492/1‑100 (MQ=255)
                tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGcc                                                                                       <  2:2224563/101‑1 (MQ=255)
                 ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.g                                                                                     <  2:6932381/101‑1 (MQ=255)
                     ctggcgcCACATGGCTTCTGCCGTCCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                                 <  2:5436539/98‑1 (MQ=37)
                          gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                            <  2:1148622/101‑1 (MQ=255)
                           ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                                           <  2:1744325/101‑1 (MQ=255)
                            cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccccc                                                                          >  2:5067835/1‑101 (MQ=255)
                            cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccccc                                                                          <  2:1462619/101‑1 (MQ=255)
                               aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                       >  2:3257553/1‑101 (MQ=255)
                                 gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggg                                                                     >  1:721962/1‑101 (MQ=255)
                                 gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGAAGCCCTGCACCAGATCGAGGAC.GGCGCTCCCGCCACggg                                                                     >  2:8933063/1‑101 (MQ=25)
                                   cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggggg                                                                   >  2:2801789/1‑101 (MQ=255)
                                     tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                                 >  2:3908683/1‑101 (MQ=255)
                                      cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                                >  2:5752823/1‑100 (MQ=255)
                                      cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCGGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                                >  1:6558027/1‑100 (MQ=255)
                                       gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                               >  2:1288169/1‑101 (MQ=255)
                                       gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                               >  2:3579016/1‑101 (MQ=255)
                                         ccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCACGCGGGGGCGTGGa                                                             <  1:1631818/101‑1 (MQ=255)
                                          cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  2:6066412/101‑1 (MQ=255)
                                          cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                            >  1:8518668/1‑101 (MQ=255)
                                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:5909007/101‑1 (MQ=255)
                                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                           >  1:3063267/1‑101 (MQ=255)
                                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                           >  2:3361429/1‑101 (MQ=255)
                                           cttcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:6595853/98‑1 (MQ=255)
                                            tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:4017903/101‑1 (MQ=255)
                                              cgcCGTCGCTCCCCCCGCGGGGGCATGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        <  2:4986149/101‑1 (MQ=255)
                                               gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  1:3043191/101‑1 (MQ=255)
                                               gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       >  1:1823574/1‑101 (MQ=255)
                                                ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                      <  2:1025133/101‑1 (MQ=255)
                                                ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                      <  2:2406370/101‑1 (MQ=255)
                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                     <  1:2900595/101‑1 (MQ=255)
                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                     <  2:2550154/101‑1 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATAGAAacac                                                    <  1:8716343/101‑1 (MQ=255)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                   >  2:5627111/1‑101 (MQ=255)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                  >  2:2366669/1‑101 (MQ=255)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                  <  2:8556086/101‑1 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                 >  1:4160121/1‑101 (MQ=255)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                                <  1:3245947/101‑1 (MQ=255)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                                               >  1:38418/1‑101 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgcc                                              >  2:2908022/1‑100 (MQ=255)
                                                        ccccccGCGGGGGCCTGGATCGAAACCTATGTGGGCTGGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacatcagct                                               <  2:7361699/101‑7 (MQ=2)
                                                         cccccGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                             <  2:9137352/101‑1 (MQ=255)
                                                             cgcgGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                         <  2:1890764/101‑1 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                       <  1:5753056/101‑1 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                       <  1:4396070/101‑1 (MQ=255)
                                                                gggggCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                      >  1:1094619/1‑101 (MQ=255)
                                                                gggggCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                      >  1:2758334/1‑101 (MQ=255)
                                                                 ggggCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                     <  2:3825159/101‑1 (MQ=255)
                                                                   ggCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                   >  1:1987994/1‑101 (MQ=255)
                                                                   ggCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                   <  2:464578/101‑1 (MQ=255)
                                                                   ggCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGCACGCGCGCACgg                                   >  1:3218882/1‑101 (MQ=255)
                                                                     cGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                 >  2:7547247/1‑101 (MQ=255)
                                                                      gTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                                >  2:6634966/1‑101 (MQ=255)
                                                                      gTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                                >  2:7949990/1‑101 (MQ=255)
                                                                        ggATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                              <  2:6385085/101‑1 (MQ=255)
                                                                        ggATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                              >  1:5665655/1‑101 (MQ=255)
                                                                           tCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcc                           >  1:1743957/1‑101 (MQ=255)
                                                                             gAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                         >  1:4731390/1‑101 (MQ=255)
                                                                             gAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                         >  1:3634492/1‑101 (MQ=255)
                                                                              aaaCGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                        >  1:7416454/1‑101 (MQ=255)
                                                                                aCGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                      <  1:3397606/101‑1 (MQ=255)
                                                                                 cGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGa                     <  2:3077080/101‑1 (MQ=255)
                                                                                  gtgtCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGGGTCAGCCCCGCGaa                    >  2:4246329/1‑101 (MQ=255)
                                                                                  gtgtCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa                    >  1:223600/1‑101 (MQ=255)
                                                                                   tgtCT.GCCACGACGCGCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                   <  2:6688716/101‑1 (MQ=255)
                                                                                   tgtCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                   <  1:5068004/101‑1 (MQ=255)
                                                                                   tgtCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCCAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                   <  1:6862022/101‑1 (MQ=255)
                                                                                    gtCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                  >  1:8065802/1‑101 (MQ=255)
                                                                                    gtCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                  >  1:4675557/1‑101 (MQ=255)
                                                                                      cT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                >  2:1599019/1‑101 (MQ=255)
                                                                                       t.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg               >  1:2689786/1‑101 (MQ=255)
                                                                                         gCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc              >  2:8382665/1‑101 (MQ=255)
                                                                                         gCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc              >  1:5430542/1‑101 (MQ=255)
                                                                                              gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc         <  2:785778/101‑1 (MQ=255)
                                                                                                cgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc       <  1:6700609/101‑1 (MQ=255)
                                                                                                cgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc       <  1:8567949/101‑1 (MQ=255)
                                                                                                cgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc       <  2:721952/101‑1 (MQ=255)
                                                                                                 gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg      <  1:5132671/101‑1 (MQ=255)
                                                                                                 gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg      >  1:608449/1‑101 (MQ=255)
                                                                                                   ccTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgcg    >  1:9175367/1‑101 (MQ=255)
                                                                                                     tGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg  >  1:7634564/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCACGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGG  >  NC_014034/1397117‑1397317

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG