Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,218 G→A 2.0% C367Y (TGC→AAT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2180G→A 2.0% 9.1 ‑1.9 ‑0.6406C367Y (TGC→TAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (216/181):  total (219/187)
Fisher's exact test strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.32e-01

Only 100 of 426 total aligned reads displayed.

GCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGA  >  NC_014034/1397118‑1397318
                                                                                                      |                                                                                                      
gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTg                                                                                                        >  2:6625118/1‑101 (MQ=255)
gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTg                                                                                                        <  2:8639853/101‑1 (MQ=255)
  gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCa                                                                                                      >  2:6431881/1‑101 (MQ=255)
  gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCa                                                                                                      >  1:5716131/1‑101 (MQ=255)
   tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCAc                                                                                                     <  2:4059204/101‑1 (MQ=255)
     gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCa                                                                                                   <  1:8310250/101‑1 (MQ=255)
      aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAg                                                                                                  <  1:5805216/101‑1 (MQ=255)
       tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGa                                                                                                 <  1:4983960/101‑1 (MQ=255)
        cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGT.CTGCCA.CGCCGCCCTGCACCAGac                                                                                                >  1:2375067/1‑100 (MQ=255)
         gAACCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATc                                                                                               <  2:1972789/101‑1 (MQ=255)
          aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATcc                                                                                              >  2:4395944/1‑101 (MQ=255)
          aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATcc                                                                                              <  2:3721314/101‑1 (MQ=255)
          aaaCGGCCCGCTCGCTCCACATTTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATcc                                                                                              <  2:2513854/101‑1 (MQ=37)
           aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                                             <  1:1022614/101‑1 (MQ=255)
            aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgc                                                                                            <  2:2551059/101‑1 (MQ=255)
             cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                           >  2:8690513/1‑101 (MQ=255)
                 cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.gc                                                                                      >  1:7790085/1‑100 (MQ=255)
                    cTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                                   >  2:8390667/1‑101 (MQ=255)
                     tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCt                                                                                  >  2:6861750/1‑101 (MQ=255)
                       gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcc                                                                                <  2:2375000/101‑1 (MQ=255)
                          ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                                             >  1:3393208/1‑101 (MQ=255)
                          ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                                             >  2:6656457/1‑101 (MQ=255)
                           cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccccc                                                                            >  2:5067835/1‑101 (MQ=255)
                            acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccccc                                                                           >  1:299999/1‑100 (MQ=255)
                              aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                         >  2:3257553/1‑101 (MQ=255)
                                gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggg                                                                       >  2:5693811/1‑101 (MQ=255)
                                  cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggggg                                                                     >  2:2801789/1‑101 (MQ=255)
                                     cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                                  >  2:8794744/1‑101 (MQ=255)
                                     cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                                  <  1:9016007/101‑1 (MQ=255)
                                     cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                                  <  1:6637155/101‑1 (MQ=255)
                                      gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                                 <  2:1106594/101‑1 (MQ=255)
                                      gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                                 >  2:3579016/1‑101 (MQ=255)
                                        ccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCACGCGGGGGCGTGGa                                                               <  1:1631818/101‑1 (MQ=255)
                                          gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                             >  2:3361429/1‑101 (MQ=255)
                                          gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                             >  1:3063267/1‑101 (MQ=255)
                                          gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCAGCGCC.GTCGCTCCCCCCGCGGGGGAGTGGATc                                                             >  2:8324677/1‑101 (MQ=255)
                                           tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  1:4017903/101‑1 (MQ=255)
                                            gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  2:631763/101‑1 (MQ=2)
                                             cgcCGTCGCTCCCCCCGCGGGGGCATGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  2:4986149/101‑1 (MQ=255)
                                              gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  2:3916603/1‑101 (MQ=255)
                                                cgctgccccccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac.a                                                      <  2:2306123/94‑1 (MQ=255)
                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac.a                                                      <  2:9022469/101‑1 (MQ=255)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac.ac                                                     >  1:6489635/1‑101 (MQ=255)
                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAAC.ACcg                                                   >  2:1659603/1‑101 (MQ=255)
                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcg                                                   <  1:6137403/101‑1 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcgc                                                  >  1:4160121/1‑101 (MQ=255)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcgcg                                                 <  1:3245947/101‑1 (MQ=255)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCgcgc                                                >  1:288452/1‑101 (MQ=255)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCgcgc                                                >  1:38418/1‑101 (MQ=255)
                                                       ccccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCgcgcc                                               >  2:2908022/1‑100 (MQ=255)
                                                        cccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCAc                                              <  2:9137352/101‑1 (MQ=255)
                                                        cccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCAc                                              <  1:745814/101‑1 (MQ=255)
                                                         ccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCAcg                                             <  1:878234/101‑1 (MQ=255)
                                                          cccGCGGGGGCGTGGATCGAAACCTATGAGGCCT.GGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac.atcagcttga                                             <  2:7539059/101‑10 (MQ=2)
                                                              cgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGa                                        <  1:4396070/101‑1 (MQ=255)
                                                               gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGaa                                       >  1:1094619/1‑101 (MQ=255)
                                                               gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGaa                                       >  1:2758334/1‑101 (MQ=255)
                                                               gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGaa                                       >  1:7905846/1‑101 (MQ=255)
                                                                ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAAc                                      <  2:7928454/101‑1 (MQ=255)
                                                                ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAAc                                      >  2:6180291/1‑101 (MQ=255)
                                                                ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAAc                                      <  2:3825159/101‑1 (MQ=255)
                                                                ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAAc                                      <  1:7377340/101‑1 (MQ=255)
                                                                  ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACgg                                    >  1:1987994/1‑101 (MQ=255)
                                                                  ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCCCGCACGCGCGCACgg                                    >  1:3218882/1‑101 (MQ=255)
                                                                  ggCGTGGATCGAAACGGGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACgg                                    <  2:1489738/101‑1 (MQ=255)
                                                                   gCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGc                                   <  1:6937301/101‑1 (MQ=255)
                                                                   gCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGc                                   <  2:7789752/101‑1 (MQ=255)
                                                                     gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGCACGGCgc                                 >  2:4834036/1‑100 (MQ=255)
                                                                     gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGt                                 >  2:5319261/1‑101 (MQ=255)
                                                                      tGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTc                                >  2:1168123/1‑101 (MQ=255)
                                                                      tGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTc                                >  2:6132462/1‑101 (MQ=255)
                                                                       ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCa                               <  1:8417945/101‑1 (MQ=255)
                                                                          tCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcc                            >  1:1743957/1‑101 (MQ=255)
                                                                            gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                          >  1:4731390/1‑101 (MQ=255)
                                                                            gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                          >  1:3634492/1‑101 (MQ=255)
                                                                            gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                          >  1:9190964/1‑101 (MQ=255)
                                                                            gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                          >  2:1824709/1‑101 (MQ=255)
                                                                            gAAACGCGT.CGGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACTGCGCACGCGCGAACGGCGCCAGcccc                          >  2:5914714/1‑101 (MQ=37)
                                                                             aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCCGCCCcg                         >  2:5462562/1‑101 (MQ=255)
                                                                             aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCcg                         >  2:6948432/1‑101 (MQ=255)
                                                                             aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCcg                         <  2:7698096/101‑1 (MQ=255)
                                                                             aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCcg                         >  2:8164766/1‑101 (MQ=255)
                                                                               aCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                       <  1:8137083/101‑1 (MQ=255)
                                                                                cGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGa                      <  2:3077080/101‑1 (MQ=255)
                                                                                cGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGa                      >  1:6740257/1‑101 (MQ=255)
                                                                                 gtgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGGGTCAGCCCCGCGaa                     >  2:4246329/1‑101 (MQ=255)
                                                                                  tgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                    <  1:5068004/101‑1 (MQ=255)
                                                                                   gt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                   >  1:1861308/1‑101 (MQ=255)
                                                                                   gt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                   >  1:4675557/1‑101 (MQ=255)
                                                                                    t.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt                  <  1:6360763/101‑1 (MQ=255)
                                                                                      cTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                 <  2:47477/101‑1 (MQ=255)
                                                                                       tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                >  1:2689786/1‑101 (MQ=255)
                                                                                        gCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc               >  1:5430542/1‑101 (MQ=255)
                                                                                              gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc          >  1:8595202/1‑101 (MQ=255)
                                                                                              gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc          >  2:5772592/1‑101 (MQ=255)
                                                                                              gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGCCAGCCCCGCGAACGTCGCTcccc          >  2:1374758/1‑101 (MQ=255)
                                                                                               acgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc         <  2:7252060/101‑1 (MQ=255)
                                                                                                 gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg       <  1:5132671/101‑1 (MQ=255)
                                                                                                  cccTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgc      <  1:8533677/101‑1 (MQ=255)
                                                                                                    cTGCACCAGATCCGCGCC.GTCGCTCCCCCCCCGGGGGCG.GGGTCCAAACAACCCCGCCCCCGCGACCGCCGCCACCCCCCCGACCGCCCCCcccccccggg    >  1:2234542/1‑97 (MQ=255)
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GCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGA  >  NC_014034/1397118‑1397318

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG