Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,222 C→G 2.0% T368T (ACC→ATG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2220C→G 2.0% 8.4 ‑1.9 ‑0.0400T368T (ACC→ACGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (213/176):  total (216/184)
Fisher's exact test strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.11e-01

Only 100 of 423 total aligned reads displayed.

GGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGT  >  NC_014034/1397122‑1397322
                                                                                                      |                                                                                                     
ggATCGAAACGTTCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCAcc                                                                                                       >  1:6534122/1‑101 (MQ=255)
 gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCa                                                                                                      <  1:8310250/101‑1 (MQ=255)
  aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAg                                                                                                     >  2:1631771/1‑101 (MQ=255)
    cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGAt                                                                                                   <  2:481825/101‑1 (MQ=255)
    cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGCCT.GCCC.CTGCGCCCTGCCCCCGac                                                                                                   >  2:4245914/1‑100 (MQ=25)
     gAACCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATc                                                                                                  <  2:1972789/101‑1 (MQ=255)
     gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATc                                                                                                  >  2:6300530/1‑101 (MQ=255)
       aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCcg                                                                                                >  1:5749385/1‑101 (MQ=255)
       aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCcg                                                                                                <  2:7712889/101‑1 (MQ=255)
       aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCcg                                                                                                <  1:1022614/101‑1 (MQ=255)
        aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCcgc                                                                                               <  2:2551059/101‑1 (MQ=255)
         cGTCCAGCTCTCGCCACATGTCTTCGCCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCcgcg                                                                                              <  2:3068203/101‑1 (MQ=255)
         cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCcgcg                                                                                              >  2:8690513/1‑101 (MQ=255)
         cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCcgcg                                                                                              >  1:1730559/1‑101 (MQ=255)
         cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCcgcc                                                                                              >  2:5282492/1‑100 (MQ=255)
           tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                            <  2:2224563/101‑1 (MQ=255)
           tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                            >  1:5103842/1‑101 (MQ=255)
           tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                            >  1:1299490/1‑101 (MQ=255)
            ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                                          <  1:8087313/101‑1 (MQ=255)
            ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                                          >  1:7119899/1‑101 (MQ=255)
             cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.gc                                                                                         >  1:7790085/1‑100 (MQ=255)
             cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.Gt                                                                                         >  2:437166/1‑101 (MQ=255)
              aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTCCACCAGATCCGCGCC.GTc                                                                                        >  1:4717219/1‑101 (MQ=255)
                ctggcgcCACATGGCTTCTGCCGTCCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                                      <  2:5436539/98‑1 (MQ=37)
                cTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                                      >  1:6153364/1‑101 (MQ=255)
                cTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                                      >  2:2751101/1‑101 (MQ=255)
                 tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCt                                                                                     <  1:2751183/101‑1 (MQ=255)
                  cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTc                                                                                    >  2:7830026/1‑101 (MQ=255)
                   gcgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcc                                                                                   >  1:5004771/1‑101 (MQ=255)
                     gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                                 <  1:5459569/101‑1 (MQ=255)
                     gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                                 >  2:4982801/1‑101 (MQ=255)
                      ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                                                >  1:8592028/1‑101 (MQ=255)
                        acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccccc                                                                              >  1:299999/1‑100 (MQ=255)
                        acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcg                                                                              <  1:3300705/101‑1 (MQ=255)
                          aTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcgcg                                                                            >  2:3257553/1‑101 (MQ=255)
                             tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgggg                                                                         <  1:1941566/101‑1 (MQ=255)
                              cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggggg                                                                        >  1:6826796/1‑101 (MQ=255)
                                 cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                                     >  2:8794744/1‑101 (MQ=255)
                                  gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                                    >  2:6631608/1‑101 (MQ=255)
                                     cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:5749152/101‑1 (MQ=255)
                                     cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:5893929/101‑1 (MQ=255)
                                     cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 >  1:40738/1‑101 (MQ=255)
                                      gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:1463952/101‑1 (MQ=255)
                                      gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  1:3063267/1‑101 (MQ=255)
                                        gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCT.GACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  2:4039281/1‑101 (MQ=2)
                                         cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  1:4370970/101‑1 (MQ=255)
                                           ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                           <  1:2210956/101‑1 (MQ=255)
                                           ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                           <  2:2406370/101‑1 (MQ=255)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                          <  2:9022469/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                        <  2:7119595/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                        >  2:5627111/1‑101 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                       <  1:2623190/101‑1 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                       >  2:2366669/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCgcgc                                                    >  1:2844620/1‑101 (MQ=255)
                                                   ccccccGCGGGGGCCTGGATCGAAACCTATGTGGGCT.GGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacatcagct                                                    <  2:7361699/101‑7 (MQ=2)
                                                    cccccGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                                  <  2:9137352/101‑1 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                                  <  1:745814/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCCcg                                                 >  2:5058480/1‑101 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcg                                                 <  1:878234/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcg                                                 >  2:6869288/1‑101 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGNAACACCGCGCAcgcgcg                                             >  2:2898527/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                           >  1:7905846/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                          <  1:3636505/101‑1 (MQ=255)
                                                            ggggCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                          >  2:6180291/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                          <  2:7928454/101‑1 (MQ=255)
                                                            ggggCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                          <  1:7377340/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                        <  2:464578/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGCACGCGCGCACgg                                        >  1:3218882/1‑101 (MQ=255)
                                                                cGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                      >  2:7547247/1‑101 (MQ=255)
                                                                cGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                      <  1:2319821/101‑1 (MQ=255)
                                                                 gTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                                     >  2:3679409/1‑101 (MQ=255)
                                                                 gTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                                     >  2:6683560/1‑101 (MQ=255)
                                                                  tGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                                    >  2:1168123/1‑101 (MQ=255)
                                                                  tGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                                    >  2:6132462/1‑101 (MQ=255)
                                                                   ggATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                   <  2:6385085/101‑1 (MQ=255)
                                                                   ggATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                   <  1:8417945/101‑1 (MQ=255)
                                                                   ggATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                   <  1:3908798/101‑1 (MQ=255)
                                                                   ggATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                   >  1:6390869/1‑101 (MQ=255)
                                                                     aTCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGCCAGc                                 >  1:6015073/1‑101 (MQ=255)
                                                                        gAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                              >  1:9198797/1‑101 (MQ=255)
                                                                         aaaCGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGCACGGCGTCAGACCcg                             >  2:3795028/1‑101 (MQ=255)
                                                                         aaaCGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                             >  2:8164766/1‑101 (MQ=255)
                                                                           aCGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                           <  1:3397606/101‑1 (MQ=255)
                                                                              tgtCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                        >  2:3838767/1‑101 (MQ=255)
                                                                               tgtcgtccA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                        <  2:3462796/95‑1 (MQ=255)
                                                                               gtCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                       >  1:6661132/1‑101 (MQ=255)
                                                                                    gCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                   >  2:8382665/1‑101 (MQ=255)
                                                                                      cA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc                 >  1:4463654/1‑101 (MQ=255)
                                                                                         cgacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccc               >  2:3360038/1‑101 (MQ=255)
                                                                                          gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc              >  2:1787743/1‑101 (MQ=255)
                                                                                          gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGCCAGCCCCGCGAACGTCGCTcccc              >  2:1374758/1‑101 (MQ=255)
                                                                                           acgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc             <  2:8518288/101‑1 (MQ=255)
                                                                                              cccTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgc          <  1:8533677/101‑1 (MQ=255)
                                                                                                 tGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg       >  1:5279962/1‑101 (MQ=255)
                                                                                                  gCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGCACGTCGCTCCCTCCGCGGGa      >  2:832313/1‑101 (MQ=255)
                                                                                                  gCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa      >  1:9129535/1‑101 (MQ=255)
                                                                                                    aCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc    <  1:1794126/101‑1 (MQ=255)
                                                                                                    aCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc    >  1:4876629/1‑101 (MQ=255)
                                                                                                     ccAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCGCGCACGCGCGGACGGCGTCAGCCCCGCCGACGGCGCTCCCCCCCCGGGAGCg   >  2:108133/1‑101 (MQ=37)
                                                                                                      cAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt  >  1:1089665/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
GGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCT.GCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGT  >  NC_014034/1397122‑1397322

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG