Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,223 A→T 2.0% R369* (AGA→TGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2230A→T 2.0% 12.5 ‑1.9 ‑0.3401R369* (AGA→TGA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (214/176):  total (218/183)
Fisher's exact test strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.75e-01

Only 100 of 420 total aligned reads displayed.

GATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTG  >  NC_014034/1397123‑1397323
                                                                                                      |                                                                                                     
gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCa                                                                                                       <  1:8310250/101‑1 (MQ=255)
gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCa                                                                                                       <  2:6176458/101‑1 (MQ=255)
 gtCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAg                                                                                                      <  2:3249640/100‑1 (MQ=255)
  tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGa                                                                                                     <  1:4983960/101‑1 (MQ=255)
   cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGAt                                                                                                    <  1:8538519/101‑1 (MQ=255)
   cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGAt                                                                                                    <  2:481825/101‑1 (MQ=255)
   cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGC.CTGCCC.CTGCGCCCTGCCCCCGac                                                                                                    >  2:4245914/1‑100 (MQ=25)
    gAACCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATc                                                                                                   <  2:1972789/101‑1 (MQ=255)
      aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                                                 <  1:1022614/101‑1 (MQ=255)
        cGTCCAGCTCTCGCCACATGTCTTCGCCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                               <  2:3068203/101‑1 (MQ=255)
        cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                               >  2:8690513/1‑101 (MQ=255)
          tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                             >  1:1299490/1‑101 (MQ=255)
          tCCAGCTCGCGCCACAGTTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                             <  1:1686423/101‑1 (MQ=255)
           ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                                           <  1:8087313/101‑1 (MQ=255)
           ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                                           >  1:7119899/1‑101 (MQ=255)
            cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.gc                                                                                          >  1:7790085/1‑100 (MQ=255)
            cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.Gt                                                                                          >  2:437166/1‑101 (MQ=255)
               ctggcgcCACATGGCTTCTGCCGTCCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                                       <  2:5436539/98‑1 (MQ=37)
                tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCt                                                                                      >  2:6861750/1‑101 (MQ=255)
                   cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                                   <  1:9159126/101‑1 (MQ=255)
                    gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                                  >  2:4982801/1‑101 (MQ=255)
                     ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                                                 >  2:6656457/1‑101 (MQ=255)
                       acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccccc                                                                               >  1:299999/1‑100 (MQ=255)
                       acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcg                                                                               <  2:3341444/101‑1 (MQ=255)
                            tCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCgggg                                                                          <  1:1941566/101‑1 (MQ=255)
                             cTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCggggg                                                                         >  1:6826796/1‑101 (MQ=255)
                                cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                                      >  2:8794744/1‑101 (MQ=255)
                                    cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:5749152/101‑1 (MQ=255)
                                    cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:5893929/101‑1 (MQ=255)
                                    cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:6066412/101‑1 (MQ=255)
                                     gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  1:3063267/1‑101 (MQ=255)
                                     cttcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  2:6595853/98‑1 (MQ=255)
                                      tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:4017903/101‑1 (MQ=255)
                                       gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                >  1:5022455/1‑101 (MQ=2)
                                       gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                >  2:4039281/1‑101 (MQ=2)
                                        cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              <  2:6336835/101‑1 (MQ=255)
                                          ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                            <  1:2210956/101‑1 (MQ=255)
                                          ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                            <  2:2406370/101‑1 (MQ=255)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                           >  1:5699109/1‑101 (MQ=255)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                           <  2:2550154/101‑1 (MQ=255)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                           <  2:9022469/101‑1 (MQ=255)
                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                            <  2:7024973/101‑1 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                          >  1:6489635/1‑101 (MQ=255)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                         >  2:5627111/1‑101 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAACACcg                                                        >  2:1659603/1‑101 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                        <  2:8556086/101‑1 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                        >  2:2366669/1‑101 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                                        <  1:2623190/101‑1 (MQ=255)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                       >  1:4160121/1‑101 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                                      <  1:3245947/101‑1 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                                      >  1:2730158/1‑101 (MQ=255)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                                                     >  1:4701392/1‑101 (MQ=255)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCgcgc                                                     >  1:2844620/1‑101 (MQ=255)
                                                   cccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                                   <  2:9137352/101‑1 (MQ=255)
                                                   cccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                                   <  1:745814/101‑1 (MQ=255)
                                                    ccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCCcg                                                  >  2:5058480/1‑101 (MQ=255)
                                                    ccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcg                                                  <  1:878234/101‑1 (MQ=255)
                                                     cccGCGGGGGCGTGGATCGAAACCTATGAGGCCT.GGCGGCCCGGTCGTGGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacatcagcttga                                                  <  2:7539059/101‑10 (MQ=2)
                                                       cgcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgc                                               <  2:6260234/101‑1 (MQ=255)
                                                        gcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgcgcg                                              <  2:815922/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGa                                             <  1:4396070/101‑1 (MQ=255)
                                                          gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGaa                                            >  1:7905846/1‑101 (MQ=255)
                                                           ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                           <  1:3636505/101‑1 (MQ=255)
                                                           ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                           <  2:7928454/101‑1 (MQ=255)
                                                           ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAAc                                           <  1:7377340/101‑1 (MQ=255)
                                                             ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                         <  2:464578/101‑1 (MQ=255)
                                                             ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGCACGCGCGCACgg                                         >  1:3218882/1‑101 (MQ=255)
                                                             ggCGTGGATCGAAACGGGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                                         <  2:1489738/101‑1 (MQ=255)
                                                              gCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGc                                        <  2:7789752/101‑1 (MQ=255)
                                                               cGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCg                                       <  1:2319821/101‑1 (MQ=255)
                                                                gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                                      >  2:3679409/1‑101 (MQ=255)
                                                                 tGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                                     >  2:1168123/1‑101 (MQ=255)
                                                                  ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                    >  1:6390869/1‑101 (MQ=255)
                                                                  ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                    <  1:8417945/101‑1 (MQ=255)
                                                                  ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                                    >  2:2186086/1‑101 (MQ=255)
                                                                       gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                               >  2:1824709/1‑101 (MQ=255)
                                                                       gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                               <  2:7647234/101‑1 (MQ=255)
                                                                        aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg                              <  1:475670/101‑1 (MQ=255)
                                                                          aCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgcg                            <  1:3397606/101‑1 (MQ=255)
                                                                             tgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                         >  2:3838767/1‑101 (MQ=255)
                                                                              gt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                        >  2:2857930/1‑101 (MQ=255)
                                                                              gt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                        >  1:6661132/1‑101 (MQ=255)
                                                                                   gCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                    >  1:5430542/1‑101 (MQ=255)
                                                                                   gCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                    >  2:8382665/1‑101 (MQ=255)
                                                                                    ccA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt                   <  2:5249176/101‑1 (MQ=255)
                                                                                         gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc               >  1:8595202/1‑101 (MQ=255)
                                                                                         gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGCCAGCCCCGCGAACGTCGCTcccc               >  2:1374758/1‑101 (MQ=255)
                                                                                           cgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc             <  2:7253571/101‑1 (MQ=255)
                                                                                            gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg            >  2:340059/1‑101 (MQ=255)
                                                                                            gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg            <  1:5132671/101‑1 (MQ=255)
                                                                                            gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg            >  1:608449/1‑101 (MQ=255)
                                                                                                tGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg        <  1:5804871/101‑1 (MQ=255)
                                                                                                 gCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGCACGTCGCTCCCTCCGCGGGa       >  2:832313/1‑101 (MQ=255)
                                                                                                 gCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa       <  2:2730076/101‑1 (MQ=255)
                                                                                                   aCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc     <  1:1794126/101‑1 (MQ=255)
                                                                                                    ccAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCGCGCACGCGCGGACGGCGTCAGCCCCGCCGACGGCGCTCCCCCCCCGGGAGCg    >  2:108133/1‑101 (MQ=37)
                                                                                                     cAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt   >  1:1089665/1‑101 (MQ=255)
                                                                                                     cAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt   <  2:3808324/101‑1 (MQ=255)
                                                                                                      aGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGCGCGTg  >  1:3865773/1‑101 (MQ=255)
                                                                                                      aGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg  >  1:991374/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
GATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTG  >  NC_014034/1397123‑1397323

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG