Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,228 C→A 2.2% S370S (TCC→TCA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2280C→A 2.2% 9.2 ‑1.2 ‑0.0369S370S (TCC→TCARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/6):  ref base (200/160):  total (203/166)
Fisher's exact test strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.77e-01

Only 100 of 409 total aligned reads displayed.

AAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCG  >  NC_014034/1397128‑1397328
                                                                                                      |                                                                                                      
aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATcc                                                                                                        >  1:6487641/1‑101 (MQ=255)
aaaCGGCCCGCTCGCTCCACATTTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATcc                                                                                                        <  2:2513854/101‑1 (MQ=37)
 aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                                                       <  1:1022614/101‑1 (MQ=255)
 aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                                                       <  2:7712889/101‑1 (MQ=255)
  aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgc                                                                                                      <  2:2551059/101‑1 (MQ=255)
   cGTCCAGCTCTCGCCACATGTCTTCGCCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                                     <  2:3068203/101‑1 (MQ=255)
     tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                                   <  2:2224563/101‑1 (MQ=255)
      ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                                                 >  1:7119899/1‑101 (MQ=255)
      ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.g                                                                                                 <  2:5187919/101‑1 (MQ=255)
       cAGCTCGCGCCGCATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.Gt                                                                                                <  2:3392662/101‑1 (MQ=255)
        aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTc                                                                                               >  2:7540034/1‑101 (MQ=255)
        aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTCCACCAGATCCGCGCC.GTc                                                                                               >  1:4717219/1‑101 (MQ=255)
          cTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                                             >  2:2751101/1‑101 (MQ=255)
              cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                                         <  2:4493616/101‑1 (MQ=255)
               gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccc                                                                                        >  1:3363333/1‑101 (MQ=255)
                 cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccccc                                                                                      <  2:5028662/101‑1 (MQ=255)
                  acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccccc                                                                                     >  1:299999/1‑100 (MQ=255)
                  acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcg                                                                                     <  2:3341444/101‑1 (MQ=255)
                      gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGAAGCCCTGCACCAGATCGAGGAC.GGCGCTCCCGCCACggg                                                                                 >  2:8933063/1‑101 (MQ=25)
                          tCGACCGTGCGCCGTCGCTCACCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCg                                                                             >  1:8223162/1‑101 (MQ=255)
                           cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGt                                                                            <  1:6637155/101‑1 (MQ=255)
                            gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                                           >  2:6631608/1‑101 (MQ=255)
                            gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                                           >  2:3579016/1‑101 (MQ=255)
                            gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                                           <  2:1106594/101‑1 (MQ=255)
                             gggctcgatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCT.GGCGGCCCGGTCGTAGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  2:3232621/92‑1 (MQ=0)
                               cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  1:40738/1‑101 (MQ=255)
                               cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCACCCCCCGCGGGGGCGTGGAt                                                                        >  1:7252364/1‑101 (MQ=255)
                                gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  1:3066065/1‑101 (MQ=255)
                                  gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:631763/101‑1 (MQ=2)
                                   cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:4370970/101‑1 (MQ=255)
                                    ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  2:1224887/99‑1 (MQ=2)
                                    gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  2:3916603/1‑101 (MQ=255)
                                    gcCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  2:8206163/101‑1 (MQ=255)
                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.Acc                                                              <  2:7119595/101‑1 (MQ=255)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAAC.ACcg                                                             >  2:1659603/1‑101 (MQ=255)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcg                                                             >  2:2366669/1‑101 (MQ=255)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcg                                                             <  1:2623190/101‑1 (MQ=255)
                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcgcg                                                           >  1:2730158/1‑101 (MQ=255)
                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcgcg                                                           <  1:8688753/101‑1 (MQ=255)
                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcgcg                                                           <  1:3245947/101‑1 (MQ=255)
                                            tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCgcgc                                                          >  2:8410346/1‑101 (MQ=255)
                                            tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAC.ACCgcgc                                                          >  1:2844620/1‑101 (MQ=255)
                                            cccccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCgcgc                                                          <  1:8908717/100‑1 (MQ=255)
                                             ccccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGAGCa                                                         >  2:4970009/1‑101 (MQ=255)
                                               ccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCAcg                                                       >  2:6869288/1‑101 (MQ=255)
                                                   gcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGNAAC.ACCGCGCAcgcgcg                                                   >  2:2898527/1‑101 (MQ=255)
                                                     gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGaa                                                 >  1:7905846/1‑101 (MQ=255)
                                                     gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGaa                                                 >  1:5113323/1‑101 (MQ=255)
                                                     gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGaa                                                 >  2:8653795/1‑101 (MQ=255)
                                                      ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAAc                                                <  2:3825159/101‑1 (MQ=255)
                                                       gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACg                                               <  1:8555732/101‑1 (MQ=255)
                                                       gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACg                                               <  2:4113956/101‑1 (MQ=255)
                                                       gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACg                                               >  2:8777870/1‑101 (MQ=255)
                                                        ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACgg                                              >  1:1987994/1‑101 (MQ=255)
                                                        ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCCCGCACGCGCGCACgg                                              >  1:3218882/1‑101 (MQ=255)
                                                        ggCGTGGATCGAAACGGGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACgg                                              <  2:1489738/101‑1 (MQ=255)
                                                          cGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCg                                            >  2:5804636/1‑101 (MQ=255)
                                                           gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCgg                                           >  2:6202339/1‑100 (MQ=255)
                                                           gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGt                                           >  2:6683560/1‑101 (MQ=255)
                                                           gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGt                                           >  2:7949990/1‑101 (MQ=255)
                                                            tGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTc                                          >  2:6132462/1‑101 (MQ=255)
                                                            tGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTc                                          >  2:1423765/1‑101 (MQ=255)
                                                             ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCa                                         <  2:5482176/101‑1 (MQ=255)
                                                             ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCa                                         <  1:8417945/101‑1 (MQ=255)
                                                               aTCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGc                                       >  2:9137209/1‑101 (MQ=255)
                                                               aTCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGCCAGc                                       >  1:6015073/1‑101 (MQ=255)
                                                                  gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                                    >  2:1824709/1‑101 (MQ=255)
                                                                  gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                                    <  1:1659650/101‑1 (MQ=255)
                                                                  gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                                    <  2:6521826/101‑1 (MQ=255)
                                                                  gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                                    >  1:5013492/1‑101 (MQ=255)
                                                                  gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                                    >  1:3634492/1‑101 (MQ=255)
                                                                   aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCcg                                   >  2:3730137/1‑101 (MQ=255)
                                                                   aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCcg                                   >  1:7416454/1‑101 (MQ=255)
                                                                      cGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGa                                >  1:6740257/1‑101 (MQ=255)
                                                                         gt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                             >  1:1861308/1‑101 (MQ=255)
                                                                         gt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                             >  1:8065802/1‑101 (MQ=255)
                                                                          t.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGt                            <  1:6360763/101‑1 (MQ=255)
                                                                            cTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTc                           <  2:5347164/101‑1 (MQ=255)
                                                                             tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                          >  1:1769066/1‑101 (MQ=255)
                                                                              gCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                         <  2:4354110/101‑1 (MQ=255)
                                                                                cA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc                       >  1:4463654/1‑101 (MQ=255)
                                                                                cA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc                       <  2:5715924/101‑1 (MQ=255)
                                                                                   cgacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccc                     >  1:398438/1‑101 (MQ=255)
                                                                                   cgacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccc                     >  2:1015660/1‑101 (MQ=255)
                                                                                   cgacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccc                     >  2:4766633/1‑101 (MQ=255)
                                                                                    gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc                    <  2:785778/101‑1 (MQ=255)
                                                                                     acgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc                   <  2:8359064/101‑1 (MQ=255)
                                                                                       gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg                 <  1:5282676/101‑1 (MQ=255)
                                                                                         ccTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcgcg               >  1:9175367/1‑101 (MQ=255)
                                                                                          cTGCACCAGATCCGCGCC.GTCGCTCCCCCCCCGGGGGCG.GGGTCCAAACAACCCCGCCCCCGCGACCGCCGCCACCCCCCCGACCGCCCCCcccccccggg              >  1:2234542/1‑97 (MQ=255)
                                                                                           tGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCggg             >  1:7634564/1‑101 (MQ=255)
                                                                                             cACCAGATCCGCGCC.GTCGCTCCCCCCGCGTGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg           >  1:5710963/1‑101 (MQ=255)
                                                                                              aCCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc          >  1:4876629/1‑101 (MQ=255)
                                                                                                cAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt        >  1:1089665/1‑101 (MQ=255)
                                                                                                 aGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg       >  2:2027486/1‑101 (MQ=255)
                                                                                                  gATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCCCGGGAGCGTgg      >  2:600362/1‑101 (MQ=255)
                                                                                                     ccGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc   <  1:383130/101‑1 (MQ=255)
                                                                                                     ccGCGCC.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc   <  2:729761/101‑1 (MQ=255)
                                                                                                      cgcgCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg  <  1:1288201/101‑1 (MQ=255)
                                                                                                      cgcgCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg  <  1:5538890/101‑1 (MQ=255)
                                                                                                      |                                                                                                      
AAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCG  >  NC_014034/1397128‑1397328

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG