Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,229 G→A 2.2% A371T (GCG→ACA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2290G→A 2.2% 10.4 ‑1.9 ‑0.0372A371T (GCG→ACG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (200/162):  total (202/170)
Fisher's exact test strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.28e-01

Only 100 of 403 total aligned reads displayed.

AACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGA  >  NC_014034/1397129‑1397329
                                                                                                      |                                                                                                      
aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                                                        >  1:5749385/1‑101 (MQ=255)
aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcg                                                                                                        <  1:1022614/101‑1 (MQ=255)
 aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgc                                                                                                       <  2:2551059/101‑1 (MQ=255)
  cGTCCAGCTCTCGCCACATGTCTTCGCCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                                                      <  2:3068203/101‑1 (MQ=255)
  cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCcgcc                                                                                                      >  2:5282492/1‑100 (MQ=255)
    tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                                                    >  2:3794725/1‑101 (MQ=255)
      cAGCTCGCGCCGCATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.Gt                                                                                                 <  2:3392662/101‑1 (MQ=255)
      cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.Gt                                                                                                 >  2:437166/1‑101 (MQ=255)
       aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTc                                                                                                >  2:7745349/1‑101 (MQ=255)
       aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCATGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTc                                                                                                >  1:4986309/1‑101 (MQ=255)
         ctggcgcCACATGGCTTCTGCCGTCCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                                              <  2:5436539/98‑1 (MQ=37)
         cTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGc                                                                                              >  2:2751101/1‑101 (MQ=255)
             cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccc                                                                                          <  1:9159126/101‑1 (MQ=255)
               ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccc                                                                                        >  2:6656457/1‑101 (MQ=255)
                cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTcccccc                                                                                       >  2:5067835/1‑101 (MQ=255)
                 acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTccccccc                                                                                      >  1:299999/1‑100 (MQ=255)
                 acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCcg                                                                                      <  2:3341444/101‑1 (MQ=255)
                     gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGAAGCCCTGCACCAGATCGAGGAC.GGCGCTCCCGCCACggg                                                                                  >  2:8933063/1‑101 (MQ=25)
                        ttCGACCGTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGATCCCCCCGCGGGGGc                                                                               >  2:6696957/1‑101 (MQ=255)
                          cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCGGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCgg                                                                             >  1:6558027/1‑100 (MQ=255)
                           gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                                            >  2:3579016/1‑101 (MQ=255)
                           gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTg                                                                            >  1:8140085/1‑101 (MQ=255)
                            gggctcgatcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCT.GGCGGCCCGGTCGTAGATCC.CGCCGGTCGCTCCCCCCGCGGGGGCGTg                                                                            <  2:3232621/92‑1 (MQ=0)
                              cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         <  2:5749152/101‑1 (MQ=255)
                              cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         <  2:5893929/101‑1 (MQ=255)
                               gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:1463952/101‑1 (MQ=255)
                               gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        >  1:3063267/1‑101 (MQ=255)
                               cttcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:6595853/98‑1 (MQ=255)
                                 gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  1:3331843/101‑1 (MQ=2)
                                 gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  1:3240430/101‑1 (MQ=2)
                                 gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTG.CTGACACCGCCAACAAATATGTGGTCAACACT.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       <  1:4308337/101‑1 (MQ=2)
                                  cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  2:6336835/101‑1 (MQ=255)
                                  cgcCGTCGCTCCCCCCGCGGGGGCATGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  2:4986149/101‑1 (MQ=255)
                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                   <  2:2406370/101‑1 (MQ=255)
                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                   <  1:8020563/101‑1 (MQ=255)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.Acc                                                               <  2:4004336/101‑1 (MQ=255)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.Acc                                                               <  1:7822697/101‑1 (MQ=255)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.Acc                                                               >  1:4056741/1‑101 (MQ=255)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.Acc                                                               >  2:5627111/1‑101 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcg                                                              >  2:2366669/1‑101 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcg                                                              <  1:2623190/101‑1 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcgc                                                             >  2:4158980/1‑101 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcgcg                                                            >  1:2730158/1‑101 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACcgcg                                                            <  1:8688753/101‑1 (MQ=255)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCgcgc                                                           >  2:8410346/1‑101 (MQ=255)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCgcgc                                                           >  1:4701392/1‑101 (MQ=255)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAC.ACCgcgc                                                           >  1:2844620/1‑101 (MQ=255)
                                           cccccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCgcgc                                                           <  1:8908717/100‑1 (MQ=255)
                                             cccccGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCAc                                                         <  2:6153127/101‑1 (MQ=255)
                                                 cgcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCAcgcgc                                                     <  2:1890764/101‑1 (MQ=255)
                                                 cgcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCAcgcgc                                                     <  2:6260234/101‑1 (MQ=255)
                                                  gcgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGNAAC.ACCGCGCAcgcgcg                                                    >  2:2898527/1‑101 (MQ=255)
                                                   cgGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGa                                                   <  1:4396070/101‑1 (MQ=255)
                                                    gggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGaa                                                  >  2:8653795/1‑101 (MQ=255)
                                                     ggggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAAc                                                 <  1:7377340/101‑1 (MQ=255)
                                                      gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACg                                                <  1:8555732/101‑1 (MQ=255)
                                                      gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACg                                                >  1:3581950/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACg                                                >  1:3808433/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACg                                                >  2:8777870/1‑101 (MQ=255)
                                                       ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACgg                                               <  2:464578/101‑1 (MQ=255)
                                                       ggCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACgg                                               >  1:1987994/1‑101 (MQ=255)
                                                         cGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCg                                             >  2:7547247/1‑101 (MQ=255)
                                                          gTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGCACGGCgc                                            >  2:4834036/1‑100 (MQ=255)
                                                           tGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTc                                           >  2:1168123/1‑101 (MQ=255)
                                                            ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCa                                          <  1:3908798/101‑1 (MQ=255)
                                                            ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCa                                          >  2:2186086/1‑101 (MQ=255)
                                                            ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCa                                          <  1:8417945/101‑1 (MQ=255)
                                                            ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCa                                          >  1:6390869/1‑101 (MQ=255)
                                                            ggATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCa                                          >  1:5665655/1‑101 (MQ=255)
                                                              aTCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGc                                        >  2:9137209/1‑101 (MQ=255)
                                                                 gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                                     <  1:1659650/101‑1 (MQ=255)
                                                                 gAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGcccc                                     >  2:38335/1‑101 (MQ=255)
                                                                  aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCcg                                    >  2:6948432/1‑101 (MQ=255)
                                                                  aaaCGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCcg                                    <  1:475670/101‑1 (MQ=255)
                                                                       tgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                               <  1:5068004/101‑1 (MQ=255)
                                                                       tgt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCCAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc                               <  1:6862022/101‑1 (MQ=255)
                                                                        gt.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg                              >  1:1861308/1‑101 (MQ=255)
                                                                            tGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCg                           >  1:1769066/1‑101 (MQ=255)
                                                                             gCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGc                          >  1:5430542/1‑101 (MQ=255)
                                                                                  cgacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccc                      >  1:7629254/1‑101 (MQ=255)
                                                                                   gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccc                     >  2:1787743/1‑101 (MQ=255)
                                                                                   gacgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGCCAGCCCCGCGAACGTCGCTcccc                     >  2:1374758/1‑101 (MQ=255)
                                                                                    acgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc                    <  2:7252060/101‑1 (MQ=255)
                                                                                    acgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTccccc                    <  2:8359064/101‑1 (MQ=255)
                                                                                     cgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc                   <  1:6700609/101‑1 (MQ=255)
                                                                                     cgCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcccccc                   <  2:6041901/101‑1 (MQ=255)
                                                                                      gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg                  >  1:608449/1‑101 (MQ=255)
                                                                                      gCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCcg                  <  1:5132671/101‑1 (MQ=255)
                                                                                         cTGCACCAGATCCGCGCC.GTCGCTCCCCCCCCGGGGGCG.GGGTCCAAACAACCCCGCCCCCGCGACCGCCGCCACCCCCCCGACCGCCCCCcccccccggg               >  1:2234542/1‑97 (MQ=255)
                                                                                            cACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg            >  2:7887387/1‑101 (MQ=255)
                                                                                             cccAGATCCGCGCC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc           <  1:7165778/100‑1 (MQ=255)
                                                                                              ccAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAC.ACCGCGCACGCGCGGACGGCGTCAGCCCCGCCGACGGCGCTCCCCCCCCGGGAGCg          >  2:108133/1‑101 (MQ=37)
                                                                                               cAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt         >  2:8147508/1‑101 (MQ=255)
                                                                                               cAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt         >  1:1089665/1‑101 (MQ=255)
                                                                                                aGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg        >  1:991374/1‑101 (MQ=255)
                                                                                                 gATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg       <  2:7442391/101‑1 (MQ=255)
                                                                                                    ccGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc    <  2:164079/101‑1 (MQ=255)
                                                                                                    ccGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc    >  1:308834/1‑101 (MQ=255)
                                                                                                     cgcgCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg   <  1:1288201/101‑1 (MQ=255)
                                                                                                      gcgcC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa  >  2:8215816/1‑101 (MQ=255)
                                                                                                      |                                                                                                      
AACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGT.CTGCCA.CGACGCCCTGCACCAGATCCGCGCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGA  >  NC_014034/1397129‑1397329

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG