Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,318 A→G 3.3% G400G (GGA→GGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,3180A→G 3.3% 20.8 ‑1.6 ‑0.2450G400G (GGA→GGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/12):  ref base (183/248):  total (190/260)
Fisher's exact test strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.98e-01

Only 100 of 477 total aligned reads displayed.

GCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAA  >  NC_014034/1397218‑1397418
                                                                                                      |                                                                                                     
 cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg                                                                                                      >  2:7887387/1‑101 (MQ=255)
 cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg                                                                                                      >  1:1921821/1‑101 (MQ=255)
  aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                                                                     <  1:1794126/101‑1 (MQ=255)
   ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCGC.GCACGCGCGGACGGC.GTCAGCCCCGCCGACGGCGCTCCCCCCCCGGGAGCg                                                                                                    >  2:108133/1‑101 (MQ=37)
    cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt                                                                                                   <  2:3808324/101‑1 (MQ=255)
     ggccctcgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTg                                                                                                  <  2:8290004/92‑1 (MQ=2)
      gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCCCGGGAGCGTgg                                                                                                 >  2:600362/1‑101 (MQ=255)
         ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                                              <  1:7950330/101‑1 (MQ=255)
         ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                                              <  1:3286275/101‑1 (MQ=255)
          cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                                             <  1:5538890/101‑1 (MQ=255)
          cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                                             <  1:1288201/101‑1 (MQ=255)
             gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                                                          >  1:7554328/1‑101 (MQ=255)
             gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                                                          <  1:8665532/101‑1 (MQ=255)
             gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                                                          >  1:4236439/1‑101 (MQ=255)
             gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                                                          <  1:410364/101‑1 (MQ=255)
              ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                                                                         <  2:2214834/101‑1 (MQ=255)
               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                                        <  1:448205/101‑1 (MQ=255)
                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                                                     >  2:5893385/1‑101 (MQ=255)
                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                                                     >  2:6525195/1‑101 (MQ=255)
                   gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                                    >  2:573638/1‑101 (MQ=255)
                   gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                                    >  2:6634524/1‑101 (MQ=255)
                      cgccccGCGGGGGCGGGGCTCGAAACACCGC.GCACGCGCGAACGGC.TCCAGACCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.Ac                                                                                <  1:8933484/99‑1 (MQ=37)
                      ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.Ac                                                                                >  2:765181/1‑101 (MQ=255)
                      ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.Ac                                                                                <  2:2735265/101‑1 (MQ=255)
                      ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.Ac                                                                                <  1:8795149/101‑1 (MQ=255)
                         cccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGc                                                                             >  2:3904127/1‑101 (MQ=255)
                             cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTc                                                                         >  1:480070/1‑101 (MQ=255)
                             cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTc                                                                         <  1:302245/101‑1 (MQ=255)
                              gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                        >  1:918399/1‑101 (MQ=255)
                               ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAANGGCTTT.ACAGCCTTcgc                                                                       >  2:4050083/1‑101 (MQ=255)
                                gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgcg                                                                      >  1:5005368/1‑101 (MQ=255)
                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                 <  2:7416130/101‑1 (MQ=255)
                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                 <  1:8325040/101‑1 (MQ=255)
                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                 >  1:5270235/1‑101 (MQ=255)
                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                 <  1:38335/101‑1 (MQ=255)
                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                >  1:1052614/1‑101 (MQ=255)
                                       gATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                               <  1:8143991/101‑1 (MQ=255)
                                       gATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                               >  2:6890246/1‑101 (MQ=255)
                                        aTCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAGACGGCTTT.ACAGCCTTCGAGGCCCACGc                                                              >  2:8952557/1‑101 (MQ=255)
                                         tCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGcc                                                             <  1:7040047/101‑1 (MQ=255)
                                         tCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGcc                                                             >  1:1583385/1‑101 (MQ=255)
                                          cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                            >  2:753659/1‑101 (MQ=255)
                                          cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                            >  1:9087708/1‑101 (MQ=255)
                                           gAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAg                                                           <  1:4745564/101‑1 (MQ=255)
                                            aaaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAga                                                          >  2:2047677/1‑101 (MQ=255)
                                            aaaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAga                                                          <  1:2801888/101‑1 (MQ=255)
                                             aaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgac                                                         <  1:2027547/101‑1 (MQ=255)
                                               cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgg                                                       <  1:3916718/101‑1 (MQ=255)
                                                acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgga                                                      <  2:180525/101‑1 (MQ=255)
                                                  cgc.gCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggacg                                                    <  2:5286453/101‑1 (MQ=255)
                                                   gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGt                                                   >  2:3473746/1‑101 (MQ=255)
                                                    c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTc                                                  <  2:1593028/101‑1 (MQ=255)
                                                    c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTc                                                  <  1:6069676/101‑1 (MQ=255)
                                                    c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTc                                                  <  1:8234907/101‑1 (MQ=255)
                                                      gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                                                 <  2:480062/101‑1 (MQ=255)
                                                         cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                                              <  1:3525031/101‑1 (MQ=255)
                                                            gcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccc                                           >  1:7409086/1‑101 (MQ=255)
                                                             cgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc                                          >  2:4153652/1‑101 (MQ=255)
                                                              gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                         <  2:7095036/101‑1 (MQ=255)
                                                               aaCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                                        <  2:9198375/101‑1 (MQ=255)
                                                                 cGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgcg                                      >  1:6465857/1‑101 (MQ=255)
                                                                  ggC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg                                     >  2:8581137/1‑101 (MQ=255)
                                                                    c.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                                   <  2:3063168/101‑1 (MQ=255)
                                                                    c.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                                   >  2:1856561/1‑101 (MQ=255)
                                                                      gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg                                  >  2:5138173/1‑101 (MQ=255)
                                                                       tCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGc                                 <  1:6259918/101‑1 (MQ=255)
                                                                           attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACTGA.CCCGGCAACGCCCTATGCGCTGGACGTGC.TCGCTCCCCCCGCGGGGGCGTGGa                            <  2:4369846/91‑1 (MQ=2)
                                                                             ccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGAt                           <  2:2844522/101‑1 (MQ=255)
                                                                              cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                          <  2:3230222/101‑1 (MQ=255)
                                                                              cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                          <  1:3669052/101‑1 (MQ=255)
                                                                               gcgAGCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                         <  1:2350917/101‑1 (MQ=255)
                                                                               gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                         >  1:8421890/1‑101 (MQ=255)
                                                                                cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                        <  1:4945685/101‑1 (MQ=255)
                                                                                cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                        <  2:4954157/101‑1 (MQ=255)
                                                                                cgAACGGCGCTTCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                        <  2:1674781/101‑1 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       >  1:5921175/1‑101 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       <  2:5613716/101‑1 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       <  1:6913209/101‑1 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       <  2:1346070/101‑1 (MQ=255)
                                                                                   aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                     <  2:4717070/101‑1 (MQ=255)
                                                                                   aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                     <  1:1423797/101‑1 (MQ=255)
                                                                                     gTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                   >  1:3389187/1‑101 (MQ=255)
                                                                                     gTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                   >  1:7721871/1‑101 (MQ=255)
                                                                                       cccgccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 <  1:2186148/97‑1 (MQ=255)
                                                                                       cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 >  1:5107164/1‑101 (MQ=255)
                                                                                       cGCGCCCCCCGCGGGGGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 >  1:7238240/1‑101 (MQ=255)
                                                                                        gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                <  2:8421527/101‑1 (MQ=255)
                                                                                        gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                <  2:3581842/101‑1 (MQ=255)
                                                                                           ccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAAt             >  1:5767669/1‑101 (MQ=255)
                                                                                           ccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAAt             <  2:6212972/101‑1 (MQ=255)
                                                                                           ccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAAt             <  1:6697225/101‑1 (MQ=255)
                                                                                             ccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATgg           <  2:5095686/101‑1 (MQ=255)
                                                                                              cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc          <  2:8182871/101‑11 (MQ=2)
                                                                                              cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc          <  2:8721430/101‑11 (MQ=2)
                                                                                                 gcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGatat       <  2:4614899/101‑1 (MQ=255)
                                                                                                  cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg      <  1:6634764/101‑1 (MQ=255)
                                                                                                    ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc    >  1:2471289/1‑101 (MQ=255)
                                                                                                    ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc    <  2:1964083/101‑1 (MQ=255)
                                                                                                      aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa  >  2:4005408/1‑101 (MQ=255)
                                                                                                      aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa  >  2:2676906/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
GCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAA  >  NC_014034/1397218‑1397418

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG