Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,349 C→G 1.4% R411G (CGC→GGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,3490C→G 1.4% 4.0 ‑1.4 ‑0.1440R411G (CGC→GGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (189/245):  total (190/250)
Fisher's exact test strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.85e-01

Only 100 of 467 total aligned reads displayed.

GGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGG  >  NC_014034/1397249‑1397449
                                                                                                     |                                                                                                    
ggggCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAANGGCTTT.ACAGCCTTcgc                                                                                                      >  2:4050083/1‑101 (MQ=255)
ggggCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgc                                                                                                      <  2:3865664/101‑1 (MQ=255)
 gggCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgcg                                                                                                     >  1:5005368/1‑101 (MQ=255)
    cGTGGATCGAAACCCCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGccc                                                                                                  <  2:1616156/101‑1 (MQ=255)
    cGTGGATCGAAACACCGCGCACGCGCGAACGTCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGccc                                                                                                  <  1:8154313/101‑1 (MQ=255)
    cGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGccc                                                                                                  <  2:6454771/101‑1 (MQ=255)
    cGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGccc                                                                                                  >  2:7728669/1‑101 (MQ=255)
     gTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGcccc                                                                                                 >  1:1810588/1‑101 (MQ=255)
      tGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                                                >  1:1640537/1‑101 (MQ=255)
      tGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                                                <  2:8213065/101‑1 (MQ=255)
       ggATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                                               <  2:288449/101‑1 (MQ=255)
       ggATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                                               >  1:1052614/1‑101 (MQ=255)
        gATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                                                              >  1:6553488/1‑101 (MQ=255)
        gATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                                                              >  2:4749733/1‑101 (MQ=255)
        gATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                                                              >  2:6890246/1‑101 (MQ=255)
          tCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGcc                                                                                            <  1:3579124/101‑1 (MQ=255)
          tCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGcc                                                                                            <  1:8003437/101‑1 (MQ=255)
           cGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                                                           <  2:8649941/101‑1 (MQ=255)
           cGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                                                           <  2:6553228/101‑1 (MQ=255)
            gAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAg                                                                                          <  1:5244170/101‑1 (MQ=255)
             aaaCGCCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAga                                                                                         <  2:1849445/101‑1 (MQ=255)
             aaaCACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAga                                                                                         <  1:2801888/101‑1 (MQ=255)
             aaaCACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAga                                                                                         >  2:878498/1‑101 (MQ=255)
              aaCACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAACCGGCTTT.ACAGCCTTCGCGCCCCACGCCAgac                                                                                        >  1:194572/1‑101 (MQ=255)
              aaCACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgac                                                                                        >  1:7621737/1‑101 (MQ=255)
              aaCACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgac                                                                                        <  2:3219855/101‑1 (MQ=255)
               acacCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacg                                                                                       <  1:7773387/101‑1 (MQ=255)
                cacCGGGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgg                                                                                      <  1:2908120/101‑1 (MQ=255)
                cacCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgg                                                                                      <  1:3916718/101‑1 (MQ=255)
                  ccGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggac                                                                                    <  1:3257653/101‑1 (MQ=255)
                    gcgcACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGt                                                                                  >  2:7503188/1‑101 (MQ=255)
                         cgcgcgAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                                                                             <  1:3525031/101‑1 (MQ=255)
                         cgcgcgAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                                                                             <  2:1727018/101‑1 (MQ=255)
                              gAACGGCGTCAGCCCGGCGAACGTCGCTCCCCCCGCGGGAGCTTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                                                        <  1:4689635/101‑1 (MQ=255)
                              gAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                                                        <  2:803759/101‑1 (MQ=255)
                              gAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                                                        <  1:2339523/101‑1 (MQ=255)
                              gAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                                                        >  1:3365379/1‑101 (MQ=255)
                               aaCGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                                                                       <  1:7312652/101‑1 (MQ=255)
                                aCGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGGGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgc                                                                      >  1:4763638/1‑101 (MQ=255)
                                     gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg                                                                 >  1:553459/1‑101 (MQ=255)
                                     gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg                                                                 >  2:5138173/1‑101 (MQ=255)
                                          ccccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTgg                                                            >  1:6301687/1‑101 (MQ=255)
                                          ccccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGCgg                                                            >  1:4192263/1‑101 (MQ=255)
                                            ccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                          >  1:9044691/1‑101 (MQ=255)
                                             cgcgAGCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:9154310/101‑1 (MQ=255)
                                             cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         >  1:5095855/1‑101 (MQ=255)
                                             cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:5710756/101‑1 (MQ=255)
                                             cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:3679518/101‑1 (MQ=255)
                                             cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:2099381/101‑1 (MQ=255)
                                              gcgGACGGCGCCCCCCCCGCGGGAGCGTGGATCGAAAAGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  2:1805346/101‑1 (MQ=38)
                                              gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGGTCg                                                        >  1:454071/1‑101 (MQ=255)
                                              gcgAACGTCGCTCCCCCCGCCGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGTTCg                                                        <  1:8820714/101‑1 (MQ=255)
                                               cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                       <  2:4954157/101‑1 (MQ=255)
                                               cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                       <  1:4945685/101‑1 (MQ=255)
                                                gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      >  2:4671314/1‑101 (MQ=255)
                                                gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      >  1:5921175/1‑101 (MQ=255)
                                                gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      >  1:8293242/1‑101 (MQ=255)
                                                  aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    >  1:6006766/1‑101 (MQ=255)
                                                  aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    <  1:1423797/101‑1 (MQ=255)
                                                   cGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCTAAAct                                                   >  2:1454892/1‑101 (MQ=255)
                                                   cGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                   >  2:2428237/1‑101 (MQ=255)
                                                       gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                                               >  1:8519316/1‑101 (MQ=255)
                                                        cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTCttga                                              >  2:206168/1‑99 (MQ=255)
                                                        cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa                                              >  1:665082/1‑101 (MQ=255)
                                                        cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa                                              <  2:4193995/101‑1 (MQ=255)
                                                          ccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAAt                                            >  2:6648460/1‑101 (MQ=255)
                                                            ccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATgg                                          >  2:5918080/1‑101 (MQ=255)
                                                            ccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATgg                                          <  1:2187685/101‑1 (MQ=255)
                                                             cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc                                         >  1:8353188/1‑91 (MQ=2)
                                                                gcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGatat                                      <  2:2695772/101‑1 (MQ=255)
                                                                 cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg                                     <  2:7058709/101‑1 (MQ=255)
                                                                 cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg                                     <  1:5772827/101‑1 (MQ=255)
                                                                  gggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATgg                                    >  2:1928761/1‑101 (MQ=255)
                                                                  gggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATgg                                    >  1:9119851/1‑101 (MQ=255)
                                                                    gAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCa                                  <  2:7621410/101‑1 (MQ=255)
                                                                     aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa                                 >  2:4005408/1‑101 (MQ=255)
                                                                      gCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaaa                                <  1:5319445/101‑1 (MQ=255)
                                                                        gTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAAc                              >  2:3570282/1‑101 (MQ=255)
                                                                         tGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAAcc                             >  2:4815368/1‑101 (MQ=255)
                                                                          ggATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCg                            <  1:7503512/101‑1 (MQ=255)
                                                                            aTCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTc                          <  2:7311597/101‑1 (MQ=255)
                                                                            aTCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTc                          <  1:4094412/101‑1 (MQ=255)
                                                                             tCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTcc                         >  1:675526/1‑101 (MQ=255)
                                                                             tCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTcc                         <  2:3065966/101‑1 (MQ=255)
                                                                             tCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTcc                         <  2:3422494/101‑1 (MQ=255)
                                                                                 aaCGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGa                     >  2:8380978/1‑101 (MQ=255)
                                                                                  aCGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGAc                    <  2:5067031/101‑1 (MQ=255)
                                                                                    ggCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACAt                  <  1:6929816/101‑1 (MQ=255)
                                                                                    ggCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACAt                  <  2:1269943/101‑1 (MQ=255)
                                                                                    ggCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACAt                  <  1:2462482/101‑1 (MQ=255)
                                                                                      cTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGt                >  2:6164516/1‑101 (MQ=255)
                                                                                       ttt.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTc               <  2:5305153/101‑1 (MQ=255)
                                                                                         t.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGc             >  1:1216869/1‑101 (MQ=255)
                                                                                         atctaGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCg              <  2:2245985/97‑1 (MQ=255)
                                                                                           aCAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCt            >  2:4419052/1‑101 (MQ=255)
                                                                                             aGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTcc          <  1:6180530/101‑1 (MQ=255)
                                                                                                cTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTccccc       >  1:2521000/1‑101 (MQ=255)
                                                                                                 ttCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTcccccc      <  2:8543748/101‑1 (MQ=255)
                                                                                                 ttCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTcccccc      <  2:1043540/101‑1 (MQ=255)
                                                                                                  tCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTcccccag     <  2:5509653/101‑3 (MQ=255)
                                                                                                     |                                                                                                    
GGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGG  >  NC_014034/1397249‑1397449

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG