Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,467 C→T 13.6% T450I (ACC→ATC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4670C→T13.6% 177.8 ‑1.9 0.0513T450I (ACC→ATC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (34/36):  ref base (256/187):  total (290/223)
Fisher's exact test strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 550 total aligned reads displayed.

CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACG  >  NC_014034/1397367‑1397567
                                                                                                    |                                                                                                       
cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                                                         <  1:1144611/101‑1 (MQ=255)
cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                                                         >  1:8013531/1‑101 (MQ=255)
 gTCGCTCCCCCCGCGGGGGCGTGGATCGACACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                                                                        <  1:5795327/101‑1 (MQ=255)
 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                                                                        <  2:9167458/101‑1 (MQ=255)
 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                                                                        >  1:818041/1‑101 (MQ=255)
 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                                                                        <  1:6324764/101‑1 (MQ=255)
  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCg                                                                                                       >  1:3822197/1‑101 (MQ=255)
   cGCTCCCCCCGCGGGGGTGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGTGGCGTGGATCGAAACACCG.t                                                                                                     >  1:3620055/1‑101 (MQ=255)
   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgc                                                                                                      >  1:3537442/1‑100 (MQ=255)
   cGCCCCCCCCGCGGGGGGGTGGATCGAAACTCTTCAATGGATATTGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.t                                                                                                     <  1:2932217/101‑1 (MQ=255)
    gCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.tc                                                                                                    >  1:1911839/1‑101 (MQ=255)
    gCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.tc                                                                                                    >  2:4668087/1‑101 (MQ=255)
      tCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.tcat                                                                                                  >  1:405027/1‑101 (MQ=255)
       ccccccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.tcatc                                                                                                 >  1:163760/1‑101 (MQ=255)
       ccccccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.ccatc                                                                                                 >  1:6738516/1‑101 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acc                                                                                             <  1:6094855/101‑1 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acc                                                                                             <  1:8346796/101‑1 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acc                                                                                             <  1:5687636/101‑1 (MQ=255)
           ccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acca                                                                                            <  2:4876469/101‑1 (MQ=255)
               gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                        >  2:3158522/1‑101 (MQ=255)
               gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                        >  2:1379627/1‑101 (MQ=255)
               gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                        >  2:8233893/1‑101 (MQ=255)
               gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                        >  2:7443413/1‑101 (MQ=255)
               gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                        >  1:959993/1‑101 (MQ=255)
                ggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCaa                                                                                       >  1:7283244/1‑101 (MQ=255)
                 gggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.c                                                                                     <  2:5523765/101‑1 (MQ=255)
                  ggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.cc                                                                                    >  2:346332/1‑101 (MQ=255)
                     gTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGcc                                                                                 >  1:2556846/1‑101 (MQ=255)
                      tGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGccc                                                                                >  2:7085772/1‑101 (MQ=255)
                       ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                               <  2:2758009/101‑1 (MQ=255)
                       ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                               >  2:8372700/1‑101 (MQ=255)
                       ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                               >  2:8046940/1‑101 (MQ=255)
                       ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                               >  1:6943218/1‑101 (MQ=255)
                        gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTg                                                                              <  2:5767434/101‑1 (MQ=255)
                          tCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGca                                                                            >  1:646113/1‑101 (MQ=255)
                          tCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGca                                                                            >  1:2059720/1‑101 (MQ=255)
                           cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcac                                                                           <  2:5270051/101‑1 (MQ=255)
                            gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcagc                                                                          >  2:930906/1‑99 (MQ=255)
                            gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacc                                                                          <  2:3832240/101‑1 (MQ=255)
                             aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacca                                                                         <  2:132548/101‑1 (MQ=255)
                              aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcaccac                                                                        >  1:7725226/1‑101 (MQ=255)
                                ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                      <  2:7554004/101‑1 (MQ=255)
                                 tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCgg                                                                     >  1:6161013/1‑101 (MQ=255)
                                  ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                    >  1:4410627/1‑101 (MQ=255)
                                   ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                   <  2:684703/101‑1 (MQ=255)
                                    tCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCg                                                                  >  2:3655039/1‑101 (MQ=255)
                                     cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGc                                                                 >  1:4218450/1‑101 (MQ=255)
                                         ggATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGGGTGGATCGACACACCG.TCCTC.ACCACGCCA.CCGCCCTGGCCCACCGCGCGCGccc                                                             >  2:2982721/1‑101 (MQ=25)
                                         ggATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTccc                                                             >  2:2532590/1‑101 (MQ=255)
                                          gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCG.TCATC.ACCACCCAA.CCGCGCTGCAGCACGGGTCGCTgccg                                                            >  2:4291757/1‑100 (MQ=37)
                                           atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTccccc                                                           >  2:193088/1‑101 (MQ=255)
                                             atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                         >  1:7086145/1‑101 (MQ=255)
                                              tGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcgc                                                        >  2:237378/1‑101 (MQ=255)
                                                gCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgg                                                      <  1:65521/101‑1 (MQ=255)
                                                 cAAACCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggg                                                     <  2:1563573/101‑1 (MQ=255)
                                                 cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggg                                                     >  2:2129828/1‑101 (MQ=255)
                                                  aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgggg                                                    >  1:1269270/1‑101 (MQ=255)
                                                   aaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggggg                                                   >  2:1800603/1‑101 (MQ=255)
                                                    aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGc                                                  <  1:5206832/101‑1 (MQ=255)
                                                    aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGc                                                  >  1:5523992/1‑101 (MQ=255)
                                                      gcGTCGGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGt                                                <  2:454063/100‑1 (MQ=255)
                                                        gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTgg                                              >  1:3726822/1‑101 (MQ=255)
                                                        gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTgg                                              >  1:1407227/1‑101 (MQ=255)
                                                          cgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:6452681/92‑1 (MQ=0)
                                                          cgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTgg                                              >  2:1191513/10‑101 (MQ=0)
                                                          ccGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:2726202/101‑1 (MQ=255)
                                                            gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  1:1442290/101‑1 (MQ=255)
                                                             cgACATGTCGCTCCCACCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGCCGCTCCCCCCGCGGGGGCGCGGATCGa                                         >  2:5968364/1‑101 (MQ=255)
                                                              gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  1:909260/1‑101 (MQ=255)
                                                              gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  1:3334851/1‑101 (MQ=255)
                                                              gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  2:405019/101‑1 (MQ=255)
                                                              gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  2:5005208/101‑1 (MQ=255)
                                                               aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:1015678/101‑1 (MQ=255)
                                                                cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                      <  1:2346627/101‑1 (MQ=255)
                                                                  cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                      <  2:5629895/100‑1 (MQ=2)
                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                 >  2:8686612/1‑101 (MQ=255)
                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                 <  2:665072/101‑1 (MQ=255)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.ACATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCg                                >  1:4764809/1‑101 (MQ=255)
                                                                         ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                             <  1:4619806/101‑1 (MQ=255)
                                                                           ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                           >  2:7323479/1‑101 (MQ=255)
                                                                             ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                         <  1:8256977/101‑1 (MQ=255)
                                                                              cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                        <  2:6132174/101‑1 (MQ=255)
                                                                                cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                      <  1:3473850/101‑1 (MQ=255)
                                                                                cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                      <  2:7820274/101‑1 (MQ=255)
                                                                                cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                      >  2:3580923/1‑101 (MQ=255)
                                                                                   gggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                   <  1:8581522/101‑1 (MQ=255)
                                                                                    ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                  <  1:7830359/101‑1 (MQ=255)
                                                                                      cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                >  1:1774069/1‑101 (MQ=255)
                                                                                      cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                <  2:8516106/101‑1 (MQ=255)
                                                                                      cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                <  2:7218210/101‑1 (MQ=255)
                                                                                       gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg               <  2:418744/101‑1 (MQ=255)
                                                                                        tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc              <  1:4834196/101‑1 (MQ=255)
                                                                                        tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc              >  2:8994469/1‑101 (MQ=255)
                                                                                        tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCACCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc              <  1:5968599/101‑1 (MQ=255)
                                                                                         ggATCGAAACACCG.TCATC.CCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg             <  1:1357456/101‑1 (MQ=255)
                                                                                            tCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccg          <  1:7443737/101‑1 (MQ=255)
                                                                                              gAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCcgcg        >  2:6307050/1‑101 (MQ=255)
                                                                                                  cacCG.GCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTgc    >  1:8845799/1‑100 (MQ=255)
                                                                                                   acCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc   >  1:6651072/1‑101 (MQ=255)
                                                                                                   acCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc   <  1:8704628/101‑1 (MQ=255)
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CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACG  >  NC_014034/1397367‑1397567

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG