Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,478 A→C 14.0% T454P (ACG→CCG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4780A→C14.0% 181.0 ‑2.4 ‑1.3502T454P (ACG→CCG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (34/37):  ref base (255/176):  total (289/213)
Fisher's exact test strand distribution p-value = 9.17e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.08e-02

Only 100 of 546 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCC  >  NC_014034/1397378‑1397578
                                                                                                      |                                                                                                      
ccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acca                                                                                                        <  2:4876469/101‑1 (MQ=255)
  gcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACg                                                                                                      <  2:308831/101‑1 (MQ=255)
     ggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCaa                                                                                                   >  1:3688781/1‑101 (MQ=255)
       ggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.cc                                                                                                <  1:1374790/101‑1 (MQ=255)
          gTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGcc                                                                                             >  2:225388/1‑101 (MQ=255)
            ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.CCCACGCAA.CCGCCC.t                                                                                          >  1:9183226/1‑101 (MQ=255)
             gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.Tg                                                                                         >  1:6608859/1‑101 (MQ=255)
             gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.Tg                                                                                         <  2:5767434/101‑1 (MQ=255)
             gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.Tg                                                                                         <  1:2773951/101‑1 (MQ=255)
                 gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcagc                                                                                     >  2:930906/1‑99 (MQ=255)
                 gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcacc                                                                                     >  1:1715596/1‑101 (MQ=255)
                  aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcacca                                                                                    <  2:132548/101‑1 (MQ=255)
                   aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcaccac                                                                                   >  1:684713/1‑101 (MQ=255)
                   aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcaccac                                                                                   <  1:5918315/101‑1 (MQ=255)
                    aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCaccacc                                                                                  <  1:4022396/101‑1 (MQ=255)
                     ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCg                                                                                 <  2:7554004/101‑1 (MQ=255)
                     ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCg                                                                                 <  2:2471216/101‑1 (MQ=255)
                      tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCgg                                                                                >  1:2635579/1‑101 (MQ=255)
                      tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCgg                                                                                >  1:3780128/1‑101 (MQ=255)
                       ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGt                                                                               <  2:3885730/101‑1 (MQ=255)
                        ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTc                                                                              <  2:9087286/101‑1 (MQ=255)
                        ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTc                                                                              >  2:7140101/1‑101 (MQ=255)
                          cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGc                                                                            >  2:8457779/1‑101 (MQ=255)
                           aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGcc                                                                           >  1:8001787/1‑100 (MQ=255)
                           aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCt                                                                           >  1:1419674/1‑101 (MQ=255)
                            aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTc                                                                          >  1:6075116/1‑101 (MQ=255)
                            aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTc                                                                          >  1:7218514/1‑101 (MQ=255)
                             tGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTcc                                                                         <  1:4773753/101‑1 (MQ=255)
                              ggATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.CCATC.CCCACGAGA.CCCCCC.GGCACCACCTGCGGCTccc                                                                        >  1:7337387/1‑101 (MQ=21)
                               gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTcccc                                                                       >  1:2013500/1‑101 (MQ=255)
                                atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTccccc                                                                      <  1:1318630/101‑1 (MQ=255)
                                atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTccccc                                                                      >  2:8650405/1‑101 (MQ=255)
                                 tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTcccccc                                                                     >  2:2695185/1‑101 (MQ=255)
                                    ggCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCcgcg                                                                  >  1:8769259/1‑101 (MQ=255)
                                     gCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCgg                                                                 <  2:7434387/101‑1 (MQ=255)
                                      cAAACCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCggg                                                                <  2:1563573/101‑1 (MQ=255)
                                       aaacCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCgggg                                                               <  2:7802534/101‑1 (MQ=255)
                                             gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTgg                                                         >  2:6397171/1‑101 (MQ=255)
                                             gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TAATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCACCGCGGGTGCGTgg                                                         >  1:3557090/1‑101 (MQ=255)
                                              ccgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTg                                                          <  2:3506456/91‑1 (MQ=0)
                                               cgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTgg                                                         >  1:1875214/10‑101 (MQ=0)
                                                gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAACCCGCAA.TCGCCCGGGCTCGATC.GCCGCTCCCCCCCCCGGGGCGTGGa                                                        >  1:4263691/9‑101 (MQ=0)
                                                 gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     <  2:1911779/101‑1 (MQ=255)
                                                   gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   <  2:5005208/101‑1 (MQ=255)
                                                   gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   >  1:3334851/1‑101 (MQ=255)
                                                    aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:6784595/101‑1 (MQ=255)
                                                    aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:1237239/101‑1 (MQ=255)
                                                    aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  1:1015678/101‑1 (MQ=255)
                                                    aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  1:69595/1‑101 (MQ=255)
                                                     cgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   >  2:65333/4‑101 (MQ=2)
                                                     cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                 <  2:8013191/101‑1 (MQ=255)
                                                      aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaaca                                                >  1:1148475/1‑101 (MQ=255)
                                                       cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                 >  1:449326/2‑101 (MQ=2)
                                                       cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                 <  1:6479691/100‑1 (MQ=2)
                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaccg                                                >  1:1549039/1‑98 (MQ=2)
                                                        gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacg                                                <  1:5759719/101‑2 (MQ=2)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacgg                                               >  1:4192816/1‑99 (MQ=2)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggg                                              >  1:5130801/1‑98 (MQ=2)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                            >  2:730194/1‑101 (MQ=255)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                            <  2:5970668/101‑1 (MQ=255)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                            <  1:6132699/101‑1 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgt                                            <  1:6374660/101‑6 (MQ=2)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAcccag                                          >  2:8331490/1‑99 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGa                                          >  2:4474457/1‑101 (MQ=255)
                                                              ccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcc                                          >  2:8858643/1‑94 (MQ=2)
                                                              ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                        >  1:8673905/1‑101 (MQ=255)
                                                              ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                        <  2:4056626/101‑1 (MQ=255)
                                                                ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaggtgtccag                                        >  2:8186866/1‑91 (MQ=2)
                                                                ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                        <  1:8188686/101‑10 (MQ=2)
                                                                ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                      <  1:8392014/101‑1 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                  <  2:6160776/101‑1 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                  <  1:6660129/101‑1 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                 <  1:3473850/101‑1 (MQ=255)
                                                                        gggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                              >  1:463298/1‑101 (MQ=255)
                                                                         ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                             <  1:7830359/101‑1 (MQ=255)
                                                                         ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                             >  1:2512001/1‑101 (MQ=255)
                                                                          gCGTGGATCGAAACACCG.TCATC.ACCCCGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                            <  2:7043953/101‑1 (MQ=255)
                                                                          gCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                            <  1:969503/101‑1 (MQ=255)
                                                                          gCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                            >  2:3602167/1‑101 (MQ=255)
                                                                           gtgggACCGAAACCCCG.TCAAC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                           <  2:5594951/98‑1 (MQ=38)
                                                                            gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                          <  2:418744/101‑1 (MQ=255)
                                                                             tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                         <  2:139668/101‑1 (MQ=255)
                                                                             tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                         >  2:4340321/1‑101 (MQ=255)
                                                                              ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                        <  1:8262569/101‑1 (MQ=255)
                                                                               gATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                       >  1:5585539/1‑101 (MQ=255)
                                                                                 tccaatcacCG.TCAGC.CCCACGCAC.CCGCCC.TGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccg                     <  2:3218783/95‑1 (MQ=25)
                                                                                  cGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                    >  1:2959027/1‑101 (MQ=255)
                                                                                      acacCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCCCAGCGCCgcgccg                >  2:2778097/1‑99 (MQ=255)
                                                                                        acCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc              >  2:5440617/1‑101 (MQ=255)
                                                                                        acCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc              <  2:5269321/101‑1 (MQ=255)
                                                                                         ccG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACg             <  2:3688672/101‑1 (MQ=255)
                                                                                         ccG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACg             >  2:2035298/1‑101 (MQ=255)
                                                                                           g.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc           >  1:7699059/1‑101 (MQ=255)
                                                                                              catc.aCCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc         >  1:4847879/1‑101 (MQ=255)
                                                                                               atc.aCCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCt        >  1:2326270/1‑101 (MQ=255)
                                                                                                   accacGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccc     <  1:7498079/101‑1 (MQ=255)
                                                                                                   accacGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccc     >  1:5392608/1‑101 (MQ=255)
                                                                                                    ccacGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccc    <  1:8994891/101‑1 (MQ=255)
                                                                                                     cacGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc   <  2:1777056/101‑1 (MQ=255)
                                                                                                     cacGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc   <  2:4635216/101‑1 (MQ=255)
                                                                                                      |                                                                                                      
CCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCC  >  NC_014034/1397378‑1397578

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG