Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,493 A→T 15.0% T459S (ACC→TCG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4930A→T15.0% 195.7 ‑1.7 ‑1.7464T459S (ACC→TCC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (33/36):  ref base (222/170):  total (255/209)
Fisher's exact test strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.88e-02

Only 100 of 519 total aligned reads displayed.

TCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1397393‑1397593
                                                                                                        |                                                                                                    
 cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcac                                                                                                     >  1:1339493/1‑101 (MQ=255)
 cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcac                                                                                                     >  1:7761620/1‑101 (MQ=255)
 cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcac                                                                                                     >  2:2501690/1‑101 (MQ=255)
  gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcagc                                                                                                    >  2:930906/1‑99 (MQ=255)
  gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcacc                                                                                                    >  1:8057133/1‑101 (MQ=255)
  gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcacc                                                                                                    <  2:3832240/101‑1 (MQ=255)
   aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcacca                                                                                                   <  2:8065462/101‑1 (MQ=255)
   aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGcacca                                                                                                   >  2:4151486/1‑101 (MQ=255)
     aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCaccacc                                                                                                 <  1:4022396/101‑1 (MQ=255)
      ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCg                                                                                                <  2:7554004/101‑1 (MQ=255)
       tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCgg                                                                                               >  1:6161013/1‑101 (MQ=255)
       tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCgg                                                                                               >  1:3780128/1‑101 (MQ=255)
       tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCgg                                                                                               >  2:65521/1‑101 (MQ=255)
        ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGt                                                                                              >  1:1193621/1‑101 (MQ=255)
         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTc                                                                                             <  1:3185531/101‑1 (MQ=255)
         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTc                                                                                             <  2:684703/101‑1 (MQ=255)
             aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTc                                                                                         >  1:6075116/1‑101 (MQ=255)
              tGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTcc                                                                                        <  1:4773753/101‑1 (MQ=255)
               ggATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.CCATC.CCCACGAGA.CCCCCC.GGCACCACCTGCGGCTccc                                                                                       >  1:7337387/1‑101 (MQ=21)
                gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTcccc                                                                                      >  1:8202788/1‑101 (MQ=255)
                gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACACCG.TCATC.ACCACCCAA.CCGCGC.TGCAGCACGGGTCGCTgccg                                                                                      >  2:4291757/1‑100 (MQ=37)
                    tGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCcgc                                                                                  >  1:6372160/1‑101 (MQ=255)
                     ggCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCcgcg                                                                                 >  1:8769259/1‑101 (MQ=255)
                      gCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCgg                                                                                <  1:65521/101‑1 (MQ=255)
                      gCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCgg                                                                                <  2:7434387/101‑1 (MQ=255)
                            ccGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGt                                                                          >  2:851958/1‑101 (MQ=255)
                                cgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                        <  2:6452681/92‑1 (MQ=0)
                                 gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTGGCTCCCCCCGCGGGGGCGTGGa                                                                       >  2:2917844/9‑101 (MQ=0)
                                 gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAACCCGCAA.TCGCCCGGGCTCGATC.GCCGCTCCCCCCCCCGGGGCGTGGa                                                                       >  1:4263691/9‑101 (MQ=0)
                                  gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  1:2428310/101‑1 (MQ=255)
                                   taccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  2:8200855/95‑1 (MQ=2)
                                   taccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:3467146/95‑1 (MQ=2)
                                   taccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     >  2:5666869/7‑101 (MQ=2)
                                    gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  <  2:405019/101‑1 (MQ=255)
                                    gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  <  2:5005208/101‑1 (MQ=255)
                                     aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  2:2706548/1‑101 (MQ=255)
                                     aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  1:69595/1‑101 (MQ=255)
                                     aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:1015678/101‑1 (MQ=255)
                                     aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:3532513/101‑1 (MQ=255)
                                       aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaaca                                                               >  1:1388241/1‑101 (MQ=255)
                                        tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacac                                                              >  2:6269906/1‑100 (MQ=255)
                                        cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                                >  1:449326/2‑101 (MQ=2)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaccg                                                               >  1:1549039/1‑98 (MQ=2)
                                         gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacg                                                               <  1:5759719/101‑2 (MQ=2)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacgg                                                              >  1:4192816/1‑99 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggg                                                             >  1:5130801/1‑98 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                                           <  1:7899589/101‑1 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                                           <  2:708770/101‑1 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                                           >  2:8686612/1‑101 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                                           >  1:142674/1‑101 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCg                                                          >  1:7567991/1‑101 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCg                                                          >  2:1267404/1‑101 (MQ=255)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAcccag                                                         >  2:8331490/1‑99 (MQ=255)
                                              cccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                                          <  2:234203/100‑7 (MQ=0)
                                               ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                                       >  1:8673905/1‑101 (MQ=255)
                                                cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTGCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                        <  1:6462571/101‑9 (MQ=2)
                                                cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                        >  1:9189293/1‑93 (MQ=2)
                                                cccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCa                                                      <  1:2081135/101‑1 (MQ=255)
                                                 ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                                       <  1:1824545/101‑10 (MQ=2)
                                                 ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                     >  2:4415936/1‑101 (MQ=255)
                                                 ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                     <  1:2573271/101‑1 (MQ=255)
                                                  cccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCG.GGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccagc                                                      <  2:913537/101‑11 (MQ=2)
                                                    cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                                  <  2:6132174/101‑1 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACACCG.TCATC.CCCACGCAC.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                                <  2:3518349/101‑1 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                                >  1:28899/1‑101 (MQ=255)
                                                         gggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                                             >  1:463298/1‑101 (MQ=255)
                                                           gCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                                           <  1:969503/101‑1 (MQ=255)
                                                           gCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                                           >  2:3602167/1‑101 (MQ=255)
                                                            cGTGGATCGAAACCCCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                          <  2:7282940/101‑1 (MQ=255)
                                                            cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                          >  1:2003166/1‑101 (MQ=255)
                                                             gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                                         >  1:1674260/1‑101 (MQ=255)
                                                             gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                                         >  2:529179/1‑101 (MQ=255)
                                                              tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                                        <  2:139668/101‑1 (MQ=255)
                                                              tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                                        >  2:8994469/1‑101 (MQ=255)
                                                               ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                                       >  2:1926632/1‑101 (MQ=255)
                                                               ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                                       <  1:8262569/101‑1 (MQ=255)
                                                               ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGCCCCGCCTCCgcg                                       >  2:485533/1‑101 (MQ=255)
                                                                gATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                                      >  1:5585539/1‑101 (MQ=255)
                                                                  tccaatcacCG.TCAGC.CCCACGCAC.CCGCCC.TGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccg                                    <  2:3218783/95‑1 (MQ=25)
                                                                      aaCACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCt                                <  2:4598236/101‑1 (MQ=255)
                                                                         acCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc                             >  1:6651072/1‑101 (MQ=255)
                                                                          ccG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACg                            >  1:3674345/1‑101 (MQ=255)
                                                                          ccG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACg                            <  2:3688672/101‑1 (MQ=255)
                                                                          ccG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACg                            >  2:2035298/1‑101 (MQ=255)
                                                                               catc.aCCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc                        >  1:4847879/1‑101 (MQ=255)
                                                                                atc.aCCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCt                       <  1:5919686/101‑1 (MQ=255)
                                                                                    accacGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccc                    >  1:5392608/1‑101 (MQ=255)
                                                                                     ccacGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccc                   <  1:8994891/101‑1 (MQ=255)
                                                                                      cacGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc                  <  2:1777056/101‑1 (MQ=255)
                                                                                      cacGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc                  >  1:6797670/1‑101 (MQ=255)
                                                                                       acGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc                 >  2:5530502/1‑101 (MQ=255)
                                                                                          cAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGGGCTGACGCCGCTCCCCCcgcg              >  2:5202466/1‑101 (MQ=255)
                                                                                          cAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgcg              >  2:7818327/1‑101 (MQ=255)
                                                                                          cAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgcg              >  1:8381531/1‑101 (MQ=255)
                                                                                          cAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCAGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgcg              >  2:5899784/1‑101 (MQ=255)
                                                                                                 ccc.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCg        >  1:7099717/1‑101 (MQ=255)
                                                                                                 ccc.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCg        <  1:2718576/101‑1 (MQ=255)
                                                                                                 ccc.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCg        >  2:1575672/1‑101 (MQ=255)
                                                                                                   c.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg      >  2:1344320/1‑101 (MQ=255)
                                                                                                     tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTgg     <  2:7962395/101‑1 (MQ=255)
                                                                                                        |                                                                                                    
TCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCC.TGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1397393‑1397593

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG