Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,542 T→A 7.7% M475K (ATG→AAA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5420T→A 7.7% 111.7 ‑1.9 ‑0.3575M475K (ATG→AAG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/26):  ref base (269/250):  total (291/284)
Fisher's exact test strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.07e-01

Only 100 of 602 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCC  >  NC_014034/1397442‑1397642
                                                                                                     |                                                                                                         
 accGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATg                                                                                                          <  1:1663417/100‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAATCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCCTgg                                                                                                         <  1:7230939/101‑1 (MQ=255)
   cgcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc                                                                                                        <  2:6132174/101‑1 (MQ=255)
     cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c..c                                                                                                   <  2:5755081/101‑1 (MQ=255)
     cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c..c                                                                                                   <  2:3726713/101‑1 (MQ=255)
      gggggCGTGGATCGAAACACCGTCATCACCACTCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..Cg                                                                                                  <  1:4671463/101‑1 (MQ=255)
         ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCt                                                                                               >  1:2512001/1‑101 (MQ=255)
           cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCa                                                                                             <  2:7218210/101‑1 (MQ=255)
           cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCa                                                                                             >  1:2003166/1‑101 (MQ=255)
             tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgc                                                                                           <  1:4834196/101‑1 (MQ=255)
              ggATCGAAACACCGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcg                                                                                          <  1:1357456/101‑1 (MQ=255)
               gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcgc                                                                                         >  2:1358094/1‑101 (MQ=255)
                 tccaatcacCGTCAGCCCCACGCACCCGCCCTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGcgccg                                                                                       <  2:3218783/95‑1 (MQ=25)
                 tCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGcgccg                                                                                       <  1:7443737/101‑1 (MQ=255)
                  cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGcgccgc                                                                                      >  1:2959027/1‑101 (MQ=255)
                   gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGTGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGcgccgcc                                                                                     >  2:5229607/1‑100 (MQ=255)
                   gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                     >  1:8629417/1‑101 (MQ=255)
                      acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.g                                                                                 >  1:5670027/1‑101 (MQ=255)
                        acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                              <  1:8238073/101‑1 (MQ=255)
                        acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                              >  1:2656178/1‑101 (MQ=255)
                        acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                              >  1:6651072/1‑101 (MQ=255)
                           gTCATCCCCACGCAACCGCCGTGCACCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                           <  2:8478439/101‑1 (MQ=255)
                           gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                           <  1:6525455/101‑1 (MQ=255)
                              atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATGATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGcc                                                                        >  1:4561001/1‑100 (MQ=255)
                                 accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                     <  1:5282345/101‑1 (MQ=255)
                                 accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                     >  1:5392608/1‑101 (MQ=255)
                                 accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                     <  1:4419179/101‑1 (MQ=255)
                                  ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                    <  1:8994891/101‑1 (MQ=255)
                                   cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCTAAACAAC.CCGGTCATGGC.C..CGCCTCACAGCCGCGCC.GA.TGCCGCTccccc                                                                   >  2:1267569/1‑101 (MQ=37)
                                   cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                   <  2:1777056/101‑1 (MQ=255)
                                    ccGCAACCGCCCTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                  <  2:7075912/100‑1 (MQ=255)
                                    acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                  <  1:7929186/101‑1 (MQ=255)
                                     cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                 <  2:3537335/101‑1 (MQ=255)
                                             cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCg                                                         >  2:1575672/1‑101 (MQ=255)
                                                ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                     <  2:8689713/91‑1 (MQ=2)
                                                 gaacaggatgTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  2:7626390/92‑1 (MQ=2)
                                                  catcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AAGGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGa                                                     <  1:7961883/93‑1 (MQ=0)
                                                  caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  2:456967/101‑1 (MQ=255)
                                                  caccacCGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:759854/101‑1 (MQ=255)
                                                  agccgccGGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACGGCGTAGATCTCGGT.C..TGGGTGCGC.CCGCGGT.TG.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:7771251/96‑1 (MQ=2)
                                                  aacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  2:225647/93‑1 (MQ=2)
                                                   atcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:3408173/94‑1 (MQ=0)
                                                   accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTGGCTCCCCCCGCGGGGGCGTGGATc                                                   >  2:3850774/1‑101 (MQ=255)
                                                    gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  >  1:988784/6‑101 (MQ=0)
                                                    cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCC.CCGATCCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  <  1:5893626/95‑1 (MQ=2)
                                                    cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  <  2:147275/95‑1 (MQ=2)
                                                    ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  <  2:5322311/101‑1 (MQ=255)
                                                    acaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  <  1:920632/100‑1 (MQ=255)
                                                        gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  1:8390896/99‑1 (MQ=2)
                                                        atgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                             <  2:4353875/99‑2 (MQ=2)
                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                             >  1:3672999/1‑100 (MQ=2)
                                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                             >  2:8806686/1‑101 (MQ=255)
                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                            >  2:6447517/1‑101 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                         <  1:2216633/101‑6 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                          >  2:6927780/1‑100 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                           >  1:876087/1‑99 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                           <  1:3774263/101‑3 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                          <  1:4954045/101‑1 (MQ=255)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                          <  1:6865029/101‑1 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGaaaagggccg                                        >  2:5670227/1‑94 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGATCGAAAcggggcgaa                                      >  1:1503410/1‑93 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                       <  2:6719935/101‑5 (MQ=2)
                                                               cccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                        <  2:7860802/100‑6 (MQ=0)
                                                                ccccccGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                     <  2:2017199/101‑10 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                      >  2:8063310/1‑101 (MQ=255)
                                                                 cccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                      <  2:560585/101‑8 (MQ=0)
                                                                 cccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                      <  2:1915050/101‑8 (MQ=0)
                                                                 cccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                      <  2:5851702/101‑8 (MQ=0)
                                                                 cccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGa                                     <  2:3399870/101‑1 (MQ=255)
                                                                  ccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                    >  1:2759294/1‑94 (MQ=2)
                                                                  ccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                    <  1:5141768/101‑8 (MQ=2)
                                                                   cccGCGGGGGCGTGGATCGAAACCCC.CCGAACCTGTCGG..CGGC.GAACGCCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                   <  2:5490346/101‑9 (MQ=0)
                                                                   cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                   <  1:1810780/101‑9 (MQ=0)
                                                                   cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                   <  1:7090621/101‑9 (MQ=0)
                                                                   cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                   <  1:5588757/101‑9 (MQ=0)
                                                                    ccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                  <  2:8226873/101‑1 (MQ=255)
                                                                        gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCgg                              >  2:7510270/1‑100 (MQ=255)
                                                                        gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                              <  2:9040339/101‑1 (MQ=255)
                                                                           ggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                           <  2:4701243/101‑1 (MQ=255)
                                                                           ggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                           >  2:736854/1‑101 (MQ=255)
                                                                           ggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                           <  1:1229637/101‑1 (MQ=255)
                                                                             cGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                         <  2:2556773/101‑1 (MQ=255)
                                                                             cGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                         <  2:7929976/101‑1 (MQ=255)
                                                                             cGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGAccc                         >  1:4004538/1‑101 (MQ=255)
                                                                              gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                        <  2:6608599/101‑1 (MQ=255)
                                                                               tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                       >  1:6316664/1‑101 (MQ=255)
                                                                                  aTCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                    <  1:5999456/101‑1 (MQ=255)
                                                                                  aTCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                    >  2:4893927/1‑101 (MQ=255)
                                                                                    cGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGACACATCCGCGGATCGGCGTGAcccgccccgc                  >  1:622756/1‑101 (MQ=255)
                                                                                    cGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                  >  1:3515207/1‑101 (MQ=255)
                                                                                     gAACCACC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                 <  2:2958929/101‑1 (MQ=255)
                                                                                      aaaCAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                >  2:1103476/1‑101 (MQ=255)
                                                                                      aaaCAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                >  1:4291602/1‑101 (MQ=255)
                                                                                      aaaCAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                >  1:1037424/1‑101 (MQ=255)
                                                                                         caac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGGGCGGCCCGCCCCCCCCAtg             >  2:4224299/1‑100 (MQ=37)
                                                                                          aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg            <  2:5107770/101‑1 (MQ=255)
                                                                                          aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg            >  1:2433033/1‑101 (MQ=255)
                                                                                              ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCTCGGATCGGCGTGACCCGCCCCGCCCATCGGTc         >  2:5009819/1‑101 (MQ=255)
                                                                                              ccGACCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc         <  2:7756938/101‑1 (MQ=255)
                                                                                                   cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc    <  2:2724631/101‑1 (MQ=255)
                                                                                                     |                                                                                                         
CCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCC  >  NC_014034/1397442‑1397642

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG