Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,550 C→G 5.5% L478V (CTC→GTC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5500C→G 5.5% 56.8 ‑2.0 ‑0.0561L478V (CTC→GTC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/19):  ref base (268/249):  total (284/277)
Fisher's exact test strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 596 total aligned reads displayed.

GGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGG  >  NC_014034/1397450‑1397650
                                                                                                        |                                                                                                      
gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGcc                                                                                                        <  2:9067097/101‑1 (MQ=255)
gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGcc                                                                                                        >  1:8907842/1‑101 (MQ=255)
 ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGG.C..CGCCt                                                                                                       >  2:7668925/1‑101 (MQ=255)
 ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCt                                                                                                       <  1:2706630/101‑1 (MQ=255)
 ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCt                                                                                                       >  1:2512001/1‑101 (MQ=255)
  gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTc                                                                                                      >  1:3790639/1‑101 (MQ=255)
   gtgggACCGAAACCCCGTCAACACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCa                                                                                                     <  2:5594951/98‑1 (MQ=38)
   cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCa                                                                                                     >  1:2003166/1‑101 (MQ=255)
   cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCa                                                                                                     <  2:8516106/101‑1 (MQ=255)
      ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcg                                                                                                  >  1:1500884/1‑101 (MQ=255)
       gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcgc                                                                                                 >  1:5585539/1‑101 (MQ=255)
       gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcgc                                                                                                 >  2:1358094/1‑101 (MQ=255)
           gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGTGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGcgccgcc                                                                                             >  2:5229607/1‑100 (MQ=255)
           gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                             >  2:6307050/1‑101 (MQ=255)
             aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCt                                                                                           >  1:4682910/1‑101 (MQ=255)
                acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                                      <  1:8238073/101‑1 (MQ=255)
                acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                                      >  1:6651072/1‑101 (MQ=255)
                   gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                   <  1:6525455/101‑1 (MQ=255)
                   ctcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                   <  2:4218332/100‑1 (MQ=255)
                     catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGc                                                                                 >  2:3357301/1‑101 (MQ=255)
                     catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGc                                                                                 >  1:4847879/1‑101 (MQ=255)
                      atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATGATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGcc                                                                                >  1:4561001/1‑100 (MQ=255)
                         accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                             <  1:4419179/101‑1 (MQ=255)
                         accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                             <  1:5282345/101‑1 (MQ=255)
                         accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                             <  1:7498079/101‑1 (MQ=255)
                           cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCTAAACAAC.CCGGTCATGGC.C..CGCCTCACAGCCGCGCC.GA.TGCCGCTccccc                                                                           >  2:1267569/1‑101 (MQ=37)
                           cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                           <  2:1777056/101‑1 (MQ=255)
                            acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                          <  1:7929186/101‑1 (MQ=255)
                              gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                        <  1:1612413/101‑1 (MQ=255)
                                    gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                  >  2:6489857/1‑101 (MQ=255)
                                     cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCg                                                                 >  2:1575672/1‑101 (MQ=255)
                                     cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCg                                                                 >  1:7099717/1‑101 (MQ=255)
                                       cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                               >  2:8229668/1‑101 (MQ=255)
                                        tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  2:7962395/101‑1 (MQ=255)
                                         gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTggc                                                             >  2:1138544/1‑100 (MQ=255)
                                          catcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AAGGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  1:7961883/93‑1 (MQ=0)
                                          agccgccGGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACGGCGTAGATCTCGGT.C..TGGGTGCGC.CCGCGGT.TG.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  1:7771251/96‑1 (MQ=2)
                                          aacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:245186/93‑1 (MQ=2)
                                          aacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:225647/93‑1 (MQ=2)
                                           cgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:2664082/95‑1 (MQ=0)
                                           accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTGGCTCCCCCCGCGGGGGCGTGGATc                                                           >  2:3850774/1‑101 (MQ=255)
                                           accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           >  1:5023549/1‑101 (MQ=255)
                                            cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  2:147275/95‑1 (MQ=2)
                                               ccGGCCGCTCCCCCCGCGGGGGCGTGGATCGAACCACC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  2:5653395/101‑1 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATGGAAACAt                                                    <  1:8680354/101‑1 (MQ=2)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                    <  1:6876012/101‑1 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                   <  1:965371/101‑1 (MQ=255)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                 <  1:2216633/101‑6 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                  >  1:9191029/1‑100 (MQ=2)
                                                     gccccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                 <  1:3546170/98‑1 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                <  2:6738245/101‑7 (MQ=2)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                  >  1:2993132/1‑98 (MQ=2)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                  <  2:3110522/101‑4 (MQ=2)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATccc                                                 >  2:5750117/1‑100 (MQ=255)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                <  2:4350410/101‑4 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAcggggcgaa                                              >  2:8705216/1‑93 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                               >  2:4692742/1‑97 (MQ=2)
                                                       cccccccGCGGGGGCGTGGATCGAACCACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                               <  2:8862902/100‑5 (MQ=0)
                                                        ccccccGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                             <  2:2017199/101‑10 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                               >  1:3468573/1‑95 (MQ=0)
                                                        ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                              >  2:8119571/1‑101 (MQ=255)
                                                         cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                             <  2:7803891/101‑7 (MQ=2)
                                                         cccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                              <  2:5851702/101‑8 (MQ=0)
                                                         cccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                              <  2:1915050/101‑8 (MQ=0)
                                                           cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                           <  1:1810780/101‑9 (MQ=0)
                                                           cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                           <  2:6884914/101‑9 (MQ=0)
                                                               cgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                       >  2:7052353/1‑101 (MQ=255)
                                                                gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCgg                                      >  2:7510270/1‑100 (MQ=255)
                                                                gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                      <  1:5893620/101‑1 (MQ=255)
                                                                gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                      >  1:844823/1‑101 (MQ=255)
                                                                gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                      >  1:7304258/1‑101 (MQ=255)
                                                                   ggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                   >  2:736854/1‑101 (MQ=255)
                                                                     cGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                 <  2:2877294/101‑1 (MQ=255)
                                                                     cGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGAccc                                 >  1:4004538/1‑101 (MQ=255)
                                                                       tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                               >  1:5650903/1‑101 (MQ=255)
                                                                       tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                               >  1:6316664/1‑101 (MQ=255)
                                                                        ggATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCcccgcc                              >  2:1012637/1‑101 (MQ=255)
                                                                        ggATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                              >  2:7886270/1‑101 (MQ=255)
                                                                        ggATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                              >  1:5388558/1‑101 (MQ=255)
                                                                          aTCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                            <  2:3510741/101‑1 (MQ=255)
                                                                          aTCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                            <  1:3679918/101‑1 (MQ=255)
                                                                          aTCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                            <  1:5999456/101‑1 (MQ=255)
                                                                            cGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGACACATCCGCGGATCGGCGTGAcccgccccgc                          >  1:622756/1‑101 (MQ=255)
                                                                            cGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                          >  1:5157100/1‑101 (MQ=255)
                                                                             gAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                         <  2:530789/101‑1 (MQ=255)
                                                                              aaaCAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                        >  1:4291602/1‑101 (MQ=255)
                                                                              aaaCAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                        >  1:1037424/1‑101 (MQ=255)
                                                                               aacaac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                       >  2:7145974/1‑101 (MQ=255)
                                                                                  aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                    <  2:6654708/101‑1 (MQ=255)
                                                                                  aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                    >  1:2433033/1‑101 (MQ=255)
                                                                                  aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                    <  2:5107770/101‑1 (MQ=255)
                                                                                      ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCTCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                 >  2:5009819/1‑101 (MQ=255)
                                                                                      ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                 <  1:119759/101‑1 (MQ=255)
                                                                                       cGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTcc                >  2:3695128/1‑100 (MQ=255)
                                                                                       cGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                >  1:7677695/1‑101 (MQ=255)
                                                                                        gATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGc               >  2:4863792/1‑101 (MQ=255)
                                                                                          tCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc             >  2:5462437/1‑101 (MQ=255)
                                                                                            aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc           >  1:1345103/1‑101 (MQ=255)
                                                                                               gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc        >  2:456388/1‑101 (MQ=255)
                                                                                                        cTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg  <  1:2786701/101‑1 (MQ=255)
                                                                                                        |                                                                                                      
GGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGG  >  NC_014034/1397450‑1397650

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG