Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,553 A→C 7.9% S479R (AGC→CGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5530A→C 7.9% 96.5 ‑2.2 ‑0.0549S479R (AGC→CGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/27):  ref base (262/241):  total (280/269)
Fisher's exact test strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.29e-01

Only 100 of 580 total aligned reads displayed.

CGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397453‑1397653
                                                                                                        |                                                                                                      
gtgggACCGAAACCCCGTCAACACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCa                                                                                                        <  2:5594951/98‑1 (MQ=38)
 gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAg                                                                                                       >  1:1674260/1‑101 (MQ=255)
 gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAg                                                                                                       >  2:529179/1‑101 (MQ=255)
  tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgc                                                                                                      <  2:139668/101‑1 (MQ=255)
   ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcg                                                                                                     >  1:1500884/1‑101 (MQ=255)
   ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGCC.C..CGCCTCCgcg                                                                                                     >  2:485533/1‑101 (MQ=255)
        gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                                >  2:6307050/1‑101 (MQ=255)
        gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                                >  1:2370326/1‑101 (MQ=255)
             acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                                         <  1:8704628/101‑1 (MQ=255)
             acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                                         <  1:8238073/101‑1 (MQ=255)
             acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                                         >  2:5440617/1‑101 (MQ=255)
                gTCATCCCCACGCAACCGCCGTGCACCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                      <  2:8478439/101‑1 (MQ=255)
                      accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                                <  1:7498079/101‑1 (MQ=255)
                       ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                               >  1:2505034/1‑101 (MQ=255)
                         acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                             <  1:7929186/101‑1 (MQ=255)
                           gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                           >  2:2882073/1‑101 (MQ=255)
                            cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..AGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                          >  2:5899784/1‑101 (MQ=255)
                                 gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                     >  2:6489857/1‑101 (MQ=255)
                                  cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCg                                                                    <  1:2718576/101‑1 (MQ=255)
                                  cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCg                                                                    >  1:7099717/1‑101 (MQ=255)
                                    cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                  >  2:8229668/1‑101 (MQ=255)
                                     tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  2:7962395/101‑1 (MQ=255)
                                     ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  2:8689713/91‑1 (MQ=2)
                                      gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  1:9199506/101‑1 (MQ=255)
                                       gcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  2:1311343/94‑1 (MQ=0)
                                       catcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AAGGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  1:7961883/93‑1 (MQ=0)
                                       agccgccGGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACGGCGTAGATCTCGGT.C..TGGGTGCGC.CCGCGGT.TG.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  1:7771251/96‑1 (MQ=2)
                                         gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  1:988784/6‑101 (MQ=0)
                                          caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  2:3790530/101‑1 (MQ=255)
                                          aggatcgcGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:8926549/94‑1 (MQ=2)
                                           accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:4036974/101‑1 (MQ=255)
                                            ccGGCCGCTCCCCCCGCGGGGGCGTGGATCGAACCACC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  2:5653395/101‑1 (MQ=255)
                                             cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         <  1:3692384/101‑1 (MQ=255)
                                             atgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                        <  2:4353875/99‑2 (MQ=2)
                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                        <  1:4926427/101‑2 (MQ=2)
                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                        >  2:1229605/1‑101 (MQ=255)
                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                        <  2:1500837/101‑1 (MQ=255)
                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                        <  2:5106995/101‑1 (MQ=255)
                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                        >  2:8806686/1‑101 (MQ=255)
                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                        >  1:7044748/1‑101 (MQ=255)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       <  1:2182647/101‑1 (MQ=2)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       <  1:3795137/101‑1 (MQ=255)
                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                      <  1:636466/101‑1 (MQ=2)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                     <  1:46087/101‑2 (MQ=2)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                      <  1:5591827/101‑3 (MQ=2)
                                                  gccccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                    <  1:3546170/98‑1 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                   <  2:6738245/101‑7 (MQ=2)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                   <  1:6045845/101‑7 (MQ=2)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                     >  1:2993132/1‑98 (MQ=2)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATccc                                                    >  2:5750117/1‑100 (MQ=255)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                                   <  2:106751/101‑1 (MQ=255)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                                   <  1:5089037/101‑1 (MQ=255)
                                                    tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                  >  2:4692742/1‑97 (MQ=2)
                                                     ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                  >  2:7470411/1‑95 (MQ=0)
                                                     ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                  >  1:3468573/1‑95 (MQ=0)
                                                     ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                 >  2:8119571/1‑101 (MQ=255)
                                                      cccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                <  2:7803891/101‑7 (MQ=2)
                                                       gcccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                               <  1:4739566/100‑8 (MQ=0)
                                                       ccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                               <  1:5188436/101‑8 (MQ=2)
                                                       ccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                               <  1:5440802/101‑1 (MQ=255)
                                                        cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                              <  2:1882051/101‑9 (MQ=0)
                                                        cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                              <  2:2070985/101‑9 (MQ=0)
                                                        cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                              <  2:6884914/101‑9 (MQ=0)
                                                          cgcgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                            >  1:6592994/1‑101 (MQ=255)
                                                            cgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                          >  2:7052353/1‑101 (MQ=255)
                                                             gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                         >  2:5218640/1‑101 (MQ=255)
                                                             gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                         <  2:9040339/101‑1 (MQ=255)
                                                             gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                         >  1:7304258/1‑101 (MQ=255)
                                                             gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                         >  1:844823/1‑101 (MQ=255)
                                                              ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                        >  2:3869220/1‑101 (MQ=255)
                                                                ggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                      >  1:1824953/1‑101 (MQ=255)
                                                                   gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                   >  1:5909767/1‑101 (MQ=255)
                                                                   gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCGCGGATCGGCGTGAcccc                                   >  2:3166254/1‑100 (MQ=255)
                                                                    tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                  >  1:4424096/1‑101 (MQ=255)
                                                                    tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATAGGCGTGAcccgc                                  >  2:1982920/1‑101 (MQ=255)
                                                                     ggATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                 >  1:6240506/1‑101 (MQ=255)
                                                                     ggATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                 >  2:7886270/1‑101 (MQ=255)
                                                                        tCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                              <  2:3525582/101‑1 (MQ=255)
                                                                          gAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                            >  2:7688762/1‑101 (MQ=255)
                                                                          gAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGCGACccaccccgcc                            >  2:7316263/1‑101 (MQ=255)
                                                                           aaaCAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                           >  1:4310151/1‑101 (MQ=255)
                                                                           aaaCAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                           >  1:1037424/1‑101 (MQ=255)
                                                                            aacaac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                          <  2:5007286/101‑1 (MQ=255)
                                                                            aacaac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                          >  2:7145974/1‑101 (MQ=255)
                                                                             acacc.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                         <  2:3364163/101‑1 (MQ=255)
                                                                              caac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGGGCGGCCCGCCCCCCCCAtg                        >  2:4224299/1‑100 (MQ=37)
                                                                               aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                       <  2:6654708/101‑1 (MQ=255)
                                                                               aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                       >  1:6763104/1‑101 (MQ=255)
                                                                                   ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                    >  1:5310013/1‑101 (MQ=255)
                                                                                   ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                    <  1:119759/101‑1 (MQ=255)
                                                                                    cGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                   >  1:7677695/1‑101 (MQ=255)
                                                                                      aTCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                 >  1:5676605/1‑101 (MQ=255)
                                                                                        cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCCCTcc               >  2:2322565/1‑101 (MQ=255)
                                                                                        cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc               <  2:2724631/101‑1 (MQ=255)
                                                                                         aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc              >  1:1345103/1‑101 (MQ=255)
                                                                                            gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc           >  2:456388/1‑101 (MQ=255)
                                                                                             c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg          <  2:2584656/101‑1 (MQ=255)
                                                                                             c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCcg          <  2:1675867/101‑1 (MQ=255)
                                                                                                     cTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg     <  1:2786701/101‑1 (MQ=255)
                                                                                                       cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc   <  1:1993470/101‑1 (MQ=255)
                                                                                                        |                                                                                                      
CGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397453‑1397653

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG