Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,686 T→C 1.4% L523P (CTG→GCG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6860T→C 1.4% 3.1 ‑3.3 ‑0.0411L523P (CTG→CCG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/6):  ref base (182/223):  total (182/229)
Fisher's exact test strand distribution p-value = 3.63e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.96e-01

Only 100 of 474 total aligned reads displayed.

CGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397586‑1397786
                                                                                                    |                                                                                                    
cGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttct                                                                                                      >  2:3603827/1‑101 (MQ=255)
 gTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctg                                                                                                     <  1:4403339/101‑1 (MQ=255)
 gTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctg                                                                                                     >  2:4924541/1‑101 (MQ=255)
 gTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgtactg                                                                                                     <  1:1856614/101‑1 (MQ=255)
    gATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctgttc                                                                                                  <  2:7862408/101‑1 (MQ=255)
    gATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctgttc                                                                                                  <  1:1800656/101‑1 (MQ=255)
    gATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctgttc                                                                                                  >  2:7282967/1‑101 (MQ=255)
       cGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCgg                                                                                               <  2:7085860/101‑1 (MQ=255)
        gaaccttCCGCGGATCGNCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGt                                                                                              <  2:1089647/95‑1 (MQ=255)
        gAAACATCCGCGGATCGGCGTGACCCGCCCCGCGCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGt                                                                                              >  1:6637643/1‑101 (MQ=255)
        gAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGt                                                                                              >  2:86322/1‑101 (MQ=255)
           aCATCCGCGGATCGGCGTGACTCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAAc                                                                                           <  2:8845393/101‑1 (MQ=255)
           aCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAAc                                                                                           <  2:7714719/101‑1 (MQ=255)
           aCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAAc                                                                                           <  2:2594064/101‑1 (MQ=255)
            cATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                                          <  2:3841996/101‑1 (MQ=255)
            cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                                          <  2:8357704/101‑1 (MQ=255)
            cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                                          <  2:4547339/101‑1 (MQ=255)
            cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                                          >  1:8368276/1‑101 (MQ=255)
             aTCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAg                                                                                         <  2:5909532/101‑1 (MQ=255)
                cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTc                                                                                      <  1:4815806/101‑1 (MQ=255)
                cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTc                                                                                      <  2:6868880/101‑1 (MQ=255)
                  cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGc                                                                                    >  1:1882824/1‑101 (MQ=255)
                  cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGc                                                                                    >  1:5520441/1‑101 (MQ=255)
                   ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCt                                                                                   <  2:4668188/101‑1 (MQ=255)
                      tCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccc                                                                                <  2:8202439/101‑1 (MQ=255)
                      tCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTATCAGGTCGCTccc                                                                                <  2:2003105/101‑1 (MQ=255)
                       gcgCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTcccc                                                                               <  2:1322310/99‑1 (MQ=255)
                       cGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTcccc                                                                               <  1:4615856/101‑1 (MQ=255)
                         gctggACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTcccccc                                                                             <  2:5059245/97‑1 (MQ=255)
                          ggggACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCcg                                                                            <  2:1388209/98‑1 (MQ=255)
                           gTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCcgc                                                                           <  1:4302102/101‑1 (MQ=255)
                             ggctcgccccgcccAGCGCTCGCTCGCCCCGGCGGGGCGTGGATCGAAGCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCgg                                                                         <  1:2424080/97‑1 (MQ=255)
                                 cgccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGgggc                                                                     >  2:7989939/1‑101 (MQ=255)
                                     ccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:1012344/101‑1 (MQ=255)
                                       gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  1:500337/101‑1 (MQ=255)
                                       gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               >  2:6276588/1‑101 (MQ=255)
                                        cccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              >  1:3947183/1‑101 (MQ=255)
                                        cccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              >  1:8543814/1‑101 (MQ=255)
                                         ccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:8660074/101‑1 (MQ=255)
                                         ccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  2:5219790/1‑101 (MQ=255)
                                          cATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  1:6931668/1‑101 (MQ=255)
                                            tCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  2:1186237/101‑1 (MQ=255)
                                             cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         <  2:5309829/101‑1 (MQ=255)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACtt                                                       >  1:358343/1‑101 (MQ=255)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGt                                                     >  2:1828383/1‑101 (MQ=255)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGt                                                     >  2:6512705/1‑101 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTc                                                    <  2:169637/101‑1 (MQ=255)
                                                  cccccccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTc                                                    <  1:2212728/98‑1 (MQ=255)
                                                    tCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                                  <  1:1450705/101‑1 (MQ=255)
                                                    cccccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                                  <  2:5520234/100‑1 (MQ=255)
                                                     ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                                                 <  1:3360142/101‑1 (MQ=255)
                                                       ccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcg                                               >  2:1427289/1‑101 (MQ=255)
                                                            cgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                          <  1:7886603/101‑1 (MQ=255)
                                                             gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                         >  1:7626717/1‑101 (MQ=255)
                                                             gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                         >  1:9119606/1‑101 (MQ=255)
                                                             gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                         >  2:6985303/1‑101 (MQ=255)
                                                             gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                         >  2:8511128/1‑101 (MQ=255)
                                                             gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                         >  2:2421535/1‑101 (MQ=255)
                                                               gggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                       <  2:7526796/101‑1 (MQ=255)
                                                                ggCGTGGATCGAAACTACAGTGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcgtca                                      <  1:8807091/101‑1 (MQ=255)
                                                                 gCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAg                                     <  1:6055548/101‑1 (MQ=255)
                                                                  cGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                                    >  1:1271598/1‑101 (MQ=255)
                                                                  cGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                                    <  1:6276828/101‑1 (MQ=255)
                                                                   gTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTTGTCTGACGCGCTCATCAGcc                                   >  1:4135861/1‑100 (MQ=255)
                                                                   gTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                                   >  1:7825757/1‑101 (MQ=255)
                                                                   gTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                                   >  1:1538891/1‑101 (MQ=255)
                                                                   gTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCCCTCATCAgga                                   >  2:3240331/1‑99 (MQ=255)
                                                                    tGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                  >  2:6718648/1‑101 (MQ=255)
                                                                    tGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                  <  1:8063650/101‑1 (MQ=255)
                                                                    tGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                  >  1:8196884/1‑101 (MQ=255)
                                                                     ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                 <  2:6013579/101‑1 (MQ=255)
                                                                     ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                 >  2:5743897/1‑101 (MQ=255)
                                                                       aTCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTc                               <  1:8458143/101‑1 (MQ=255)
                                                                          gAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                            <  1:3695237/101‑1 (MQ=255)
                                                                          gAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                            >  1:5713994/1‑101 (MQ=255)
                                                                            aaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt                          <  2:2656096/101‑1 (MQ=255)
                                                                             aCTGCAGGGTTCCGGTCGGGTTCTGTTCCGGTAGCAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt                         <  2:9172046/101‑1 (MQ=37)
                                                                              cTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc                        <  1:323510/101‑1 (MQ=255)
                                                                                 cAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAttacgc                     >  2:2912456/1‑97 (MQ=255)
                                                                                 cAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt                     >  1:7404892/1‑101 (MQ=255)
                                                                                  agagTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc                    <  1:5612752/101‑1 (MQ=255)
                                                                                    agTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                  >  2:4102171/1‑101 (MQ=255)
                                                                                    agTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                  >  2:5592839/1‑101 (MQ=255)
                                                                                    agTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                  <  1:1667382/101‑1 (MQ=255)
                                                                                    agTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                  >  1:5288703/1‑101 (MQ=255)
                                                                                      ttCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTc                <  1:5854256/101‑1 (MQ=255)
                                                                                          ggTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc            <  2:1538844/101‑1 (MQ=255)
                                                                                          ggTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc            <  2:222533/101‑1 (MQ=255)
                                                                                          ggTCTAGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGCCTGACGCGCTCATCAGCACGTTAAGATTCAGTGGCTctccc            >  2:402324/1‑101 (MQ=37)
                                                                                            tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg          <  2:3334747/101‑1 (MQ=255)
                                                                                            tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg          <  1:6962156/101‑1 (MQ=255)
                                                                                             cTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcgc         >  1:6477817/1‑101 (MQ=255)
                                                                                                gttctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggg      >  1:1071294/1‑101 (MQ=255)
                                                                                                 ttctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCgggg     >  2:1815752/1‑101 (MQ=255)
                                                                                                   ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGGGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc   >  2:4326643/1‑101 (MQ=255)
                                                                                                   ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc   >  1:2767369/1‑101 (MQ=255)
                                                                                                   ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc   >  1:5160915/1‑101 (MQ=255)
                                                                                                   ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc   <  1:7242179/101‑1 (MQ=255)
                                                                                                   ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc   <  1:7052638/101‑1 (MQ=255)
                                                                                                    tgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCg  >  1:3099440/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397586‑1397786

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG