Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,870 G→A 1.1% intergenic (+174/+1724) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,8700G→A 1.1% 3.7 ‑1.0 ‑0.6461intergenic (+174/+1724)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (213/242):  total (215/246)
Fisher's exact test strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.31e-01

Only 100 of 496 total aligned reads displayed.

CTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAA  >  NC_014034/1397770‑1397970
                                                                                                     |                                                                                                     
cTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCg                                                                                                       >  2:3559045/1‑101 (MQ=255)
  ccccccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGc                                                                                                     <  2:3413139/101‑1 (MQ=255)
  ccccccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGc                                                                                                     <  2:9038360/101‑1 (MQ=255)
      ccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAccc                                                                                                 >  2:8261962/1‑100 (MQ=255)
      ccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCt                                                                                                 <  1:7831589/101‑1 (MQ=255)
         cgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAAC.CCGGCACGTC.ac                                                                                             >  2:1934332/1‑100 (MQ=255)
          gggggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGc                                                                                            >  2:6412456/1‑101 (MQ=255)
           ggggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGcc                                                                                           <  1:3027044/101‑1 (MQ=255)
            gggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGccc                                                                                          >  1:2316544/1‑101 (MQ=255)
            gggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGccc                                                                                          >  2:8684354/1‑101 (MQ=255)
            gggCGTGGAGCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGccc                                                                                          <  2:1556472/101‑1 (MQ=255)
             ggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGcccc                                                                                         <  2:5130632/101‑1 (MQ=255)
             ggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGcccc                                                                                         <  2:4793839/101‑1 (MQ=255)
             ggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGcccc                                                                                         <  2:8816779/101‑1 (MQ=255)
              gCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCcccg                                                                                        <  2:5588254/101‑1 (MQ=255)
                gTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCcccgcc                                                                                      <  2:2290402/101‑1 (MQ=255)
                gTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCcccgcc                                                                                      >  1:4875846/1‑101 (MQ=255)
                 tGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCcccgccc                                                                                     <  1:8321251/101‑1 (MQ=255)
                  ggATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCcccgcccg                                                                                    >  2:8170112/1‑101 (MQ=255)
                    aTCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCt                                                                                  >  1:822180/1‑101 (MQ=255)
                    aTCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCt                                                                                  <  2:5834370/101‑1 (MQ=255)
                      cGAAACGGTGTCCAGCGTCAGATGCAGCGGCGTCGAATGGACATGGCGCTCCCCCCGCGGGGGCGGGGACCGAGAC.CCGGGAGCTCGAGCcgcgccgggga                                                                                 >  2:4258437/1‑96 (MQ=255)
                      cGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGt                                                                                >  1:4667401/1‑101 (MQ=255)
                       gAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGtc                                                                               >  1:7690706/1‑101 (MQ=255)
                       gAAACGGTGTCCAGCGTCACATGCAGCGGCGGCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGtc                                                                               <  2:7633824/101‑1 (MQ=255)
                        aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGtct                                                                              >  1:4476699/1‑101 (MQ=255)
                        aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGtct                                                                              >  1:9108237/1‑101 (MQ=255)
                        aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGtct                                                                              >  2:78761/1‑101 (MQ=255)
                         aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGtctc                                                                             <  1:2830504/101‑1 (MQ=255)
                           cGGTGTCCAGCGTCACATGCAGCGGCNNNNNATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCAc                                                                           <  1:978171/101‑1 (MQ=255)
                           cGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCAc                                                                           >  1:3399178/1‑101 (MQ=255)
                            ggTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATTGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCAcc                                                                          >  2:5322077/1‑101 (MQ=255)
                            ggTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCAcc                                                                          >  1:5052908/1‑101 (MQ=255)
                            ggTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCAcc                                                                          >  1:606277/1‑101 (MQ=255)
                             gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCa                                                                         >  2:4637657/1‑101 (MQ=255)
                             gtgtCCAGCGTCACAGGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCTCAGATCGAAAC.CTGGGACGTC.TGCCCTGCCCGCTCtccgtcca                                                                         >  2:8515153/1‑95 (MQ=25)
                               gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAAc                                                                       <  1:1037975/101‑1 (MQ=255)
                               gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAAc                                                                       <  1:151050/101‑1 (MQ=255)
                                  cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGt                                                                    <  1:6902723/101‑1 (MQ=255)
                                     cggcacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                 <  2:1189135/98‑1 (MQ=255)
                                     cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                 <  2:7067150/101‑1 (MQ=255)
                                      gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                >  1:7382780/1‑101 (MQ=255)
                                      gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                >  1:7933055/1‑101 (MQ=255)
                                      gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                <  2:4721914/101‑1 (MQ=255)
                                       gcacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                               <  2:630898/100‑1 (MQ=255)
                                        cacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTcc                                                              >  2:2651916/1‑101 (MQ=255)
                                        cacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTcc                                                              >  2:2029863/1‑101 (MQ=255)
                                         acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                             <  1:123182/101‑1 (MQ=255)
                                            tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                          <  2:723919/101‑1 (MQ=255)
                                             gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                         >  2:7519113/1‑101 (MQ=255)
                                             gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                         <  1:4282823/101‑1 (MQ=255)
                                              cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                        >  2:8395475/1‑101 (MQ=255)
                                                   gcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggggg                                                   <  2:1401223/101‑1 (MQ=255)
                                                    cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGTATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                  <  1:5186153/101‑1 (MQ=255)
                                                     gTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCg                                                 >  1:3758906/1‑101 (MQ=255)
                                                       cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                               <  1:1567453/101‑1 (MQ=255)
                                                       cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                               >  2:9200098/1‑101 (MQ=255)
                                                         cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  2:2462463/101‑1 (MQ=255)
                                                         cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  2:1764377/1‑101 (MQ=255)
                                                          aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  1:6456042/1‑101 (MQ=255)
                                                           tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:8859049/101‑1 (MQ=255)
                                                           tCGCCATGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  2:315780/101‑1 (MQ=255)
                                                            cGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  1:6201132/101‑1 (MQ=255)
                                                             gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         <  1:7064791/101‑1 (MQ=255)
                                                             gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         <  1:8502935/101‑1 (MQ=255)
                                                              gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGA.AGCCAAGGCCGCAGCAGCGGCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         >  2:6765456/5‑101 (MQ=2)
                                                              ccATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  1:2103676/1‑101 (MQ=255)
                                                                aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      <  2:449318/101‑1 (MQ=255)
                                                                aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      >  2:2204797/1‑101 (MQ=255)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                    <  1:4172024/101‑1 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTa                                  >  2:3977850/1‑101 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                 <  1:4744252/101‑1 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                <  1:361544/101‑1 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                >  1:5664809/1‑101 (MQ=255)
                                                                         cccgcGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCCTGGATCGAAACTCTATggga                             >  1:7216693/1‑101 (MQ=255)
                                                                           cccGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggagg                           <  2:4333387/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggc                          <  2:4938099/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggc                          <  1:2480450/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggc                          >  2:1265062/1‑101 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCTCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggcta                        <  1:6564037/101‑1 (MQ=255)
                                                                                 ggggCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                     >  1:3214868/1‑101 (MQ=255)
                                                                                  gggCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                    >  1:1886537/1‑101 (MQ=255)
                                                                                  gggCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                    <  2:7415691/101‑1 (MQ=255)
                                                                                  gggCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                    <  2:2773200/101‑1 (MQ=255)
                                                                                     cGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggc                 >  2:6578512/1‑101 (MQ=255)
                                                                                     cGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggc                 >  2:2553291/1‑101 (MQ=255)
                                                                                      gTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                >  1:3877012/1‑101 (MQ=255)
                                                                                      gTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                >  1:5504635/1‑101 (MQ=255)
                                                                                       tGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta               >  1:2202/1‑101 (MQ=255)
                                                                                       tGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta               >  1:5776834/1‑101 (MQ=255)
                                                                                       tGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta               <  2:3753352/101‑1 (MQ=255)
                                                                                        ggATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc              <  2:6531889/101‑1 (MQ=255)
                                                                                            cGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc          >  1:6799134/1‑101 (MQ=255)
                                                                                             gAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc         <  1:7465070/101‑1 (MQ=255)
                                                                                             gAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc         <  2:5437105/101‑1 (MQ=255)
                                                                                              aaaC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt        <  2:3011837/101‑1 (MQ=255)
                                                                                                   ccGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa    <  1:2720560/101‑1 (MQ=255)
                                                                                                   ccGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa    >  2:6193141/1‑101 (MQ=255)
                                                                                                    cGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa   <  2:7986077/101‑1 (MQ=255)
                                                                                                     ggCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa  >  2:56562/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTC.AGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAA  >  NC_014034/1397770‑1397970

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG