Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,894 T→G 1.2% intergenic (+198/+1700) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,8940T→G 1.2% 3.2 ‑0.4 ‑0.4427intergenic (+198/+1700)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (2/4):  ref base (184/236):  total (186/241)
Fisher's exact test strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.44e-01

Only 100 of 466 total aligned reads displayed.

AAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397794‑1397994
                                                                                                    |                                                                                                    
aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtct                                                                                                      >  1:9108237/1‑101 (MQ=255)
aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtct                                                                                                      >  1:4667987/1‑101 (MQ=255)
 aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGtctc                                                                                                     <  1:2830504/101‑1 (MQ=255)
   cGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCAc                                                                                                   >  1:3399178/1‑101 (MQ=255)
     gtgtCCAGCGTCCCATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                                                 >  1:6552522/1‑101 (MQ=255)
     gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                                                 >  2:7894662/1‑101 (MQ=255)
     gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                                                 >  2:2642157/1‑101 (MQ=255)
       gtCCAGCGTCACATGCAGCGGCGTCGCATCGCTATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                                               <  2:6760022/101‑1 (MQ=255)
       gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                                               <  1:1037975/101‑1 (MQ=255)
        tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                              <  2:8520843/101‑1 (MQ=255)
         ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                                             >  1:7892723/1‑101 (MQ=255)
          cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                                            <  1:6902723/101‑1 (MQ=255)
              gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                                        >  1:5001571/1‑101 (MQ=255)
               tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCGGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                                       <  2:1403549/101‑1 (MQ=255)
               tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                                       <  1:3063205/101‑1 (MQ=255)
               gcacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                                       <  2:630898/100‑1 (MQ=255)
                cacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcc                                                                                      >  2:4672719/1‑101 (MQ=255)
                 acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTTACCAACAGTCGCTacc                                                                                     >  2:7501923/1‑101 (MQ=255)
                  caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                                                    >  2:72698/1‑101 (MQ=255)
                   aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                                                   <  2:6405804/101‑1 (MQ=255)
                   aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                                                   <  2:7690380/101‑1 (MQ=255)
                    tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                                  >  2:3323678/1‑101 (MQ=255)
                    tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                                  <  2:2256894/101‑1 (MQ=255)
                    tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                                  <  2:5334719/101‑1 (MQ=255)
                     gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                                 >  1:3271014/1‑101 (MQ=255)
                      cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                                                >  2:8395475/1‑101 (MQ=255)
                      cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                                                <  1:4803892/101‑1 (MQ=255)
                         cggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggg                                                                             >  2:250513/1‑101 (MQ=255)
                         cggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCCCCCCCCGCggg                                                                             >  2:35198/1‑101 (MQ=255)
                               cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                                       >  2:9200098/1‑101 (MQ=255)
                                gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  1:6764780/101‑1 (MQ=255)
                                 cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  2:1764377/1‑101 (MQ=255)
                                 cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:5125085/1‑101 (MQ=255)
                                  aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCCCCGGGGCGTgggt                                                                    >  2:5763913/1‑99 (MQ=255)
                                   tCGCCATGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:315780/101‑1 (MQ=255)
                                    cGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  1:7429799/1‑101 (MQ=255)
                                     gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 <  1:7064791/101‑1 (MQ=255)
                                       cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               >  2:6522727/1‑101 (MQ=255)
                                        aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  2:9027780/101‑1 (MQ=255)
                                        aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  2:8101526/1‑101 (MQ=255)
                                         tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                             >  2:2379927/1‑101 (MQ=255)
                                         tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                             >  1:4304996/1‑101 (MQ=255)
                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                            <  1:4172024/101‑1 (MQ=255)
                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                            <  1:3851227/101‑1 (MQ=255)
                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                            >  1:6002987/1‑101 (MQ=255)
                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                                           >  2:8802439/1‑101 (MQ=255)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTa                                                          >  2:3977850/1‑101 (MQ=255)
                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                                         <  2:831866/101‑1 (MQ=255)
                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                                         <  1:4744252/101‑1 (MQ=255)
                                              cTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                                        <  2:286447/101‑1 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                                                      <  2:2720232/101‑1 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                                                      >  1:6196304/1‑101 (MQ=255)
                                                  ccccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                                                    >  1:394473/1‑101 (MQ=255)
                                                   cccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggagg                                                   <  2:1197381/101‑1 (MQ=255)
                                                    ccGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggc                                                  <  2:2067414/101‑1 (MQ=255)
                                                     cgcgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggct                                                 <  1:1234801/101‑1 (MQ=255)
                                                       cgGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                               >  1:1565268/1‑101 (MQ=255)
                                                        gggggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                                              >  1:727807/1‑101 (MQ=255)
                                                          gggCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                                            >  1:1886537/1‑101 (MQ=255)
                                                            gCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggagg                                          <  1:8106079/101‑1 (MQ=255)
                                                              gTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                        >  1:5504635/1‑101 (MQ=255)
                                                              gTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                        <  1:8491709/101‑1 (MQ=255)
                                                              gTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                        <  2:1316266/101‑1 (MQ=255)
                                                               tGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                       <  1:4506013/101‑1 (MQ=255)
                                                               tGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                       <  2:3753352/101‑1 (MQ=255)
                                                               tGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                       >  1:5776834/1‑101 (MQ=255)
                                                               tGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                       >  1:2202/1‑101 (MQ=255)
                                                                ggATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                                      <  1:2380000/101‑1 (MQ=255)
                                                                   tCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGCGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCa                                   <  2:4429317/101‑1 (MQ=255)
                                                                    cGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc                                  >  1:3686617/1‑101 (MQ=255)
                                                                     gAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc                                 <  2:5437105/101‑1 (MQ=255)
                                                                      aaaCCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                                <  2:3011837/101‑1 (MQ=255)
                                                                        accgcgCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTc                              <  2:7579396/96‑1 (MQ=255)
                                                                        aCCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTc                              <  2:4551583/101‑1 (MQ=255)
                                                                        aCCCGGCACCTCAGCCCCGCCCACTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTc                              <  1:8296454/101‑1 (MQ=255)
                                                                          ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa                            <  1:2720560/101‑1 (MQ=255)
                                                                           cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGCGGCTGGGGAGGCTACGCACCTTCaaa                           >  2:4914264/1‑101 (MQ=255)
                                                                           cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                           <  1:6410894/101‑1 (MQ=255)
                                                                             gCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAg                         <  2:423472/101‑1 (MQ=255)
                                                                               aCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTc                       <  2:5086793/101‑1 (MQ=255)
                                                                               aCCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTc                       <  2:1881800/101‑1 (MQ=255)
                                                                                 cTCAGCCCCGCCCGCTGTCTGGCCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCTCCTTCAAAAGTCGc                     <  1:5630551/101‑1 (MQ=255)
                                                                                 cTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGc                     <  1:1468533/101‑1 (MQ=255)
                                                                                   cAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTc                   >  1:2554180/1‑101 (MQ=255)
                                                                                       cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc               <  1:1345849/101‑1 (MQ=255)
                                                                                       cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc               <  1:2090110/101‑1 (MQ=255)
                                                                                       cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc               <  2:6937207/101‑1 (MQ=255)
                                                                                       cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc               <  2:6173010/101‑1 (MQ=255)
                                                                                        ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccccc              <  1:1788070/101‑1 (MQ=255)
                                                                                        ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccccc              >  2:552400/1‑101 (MQ=255)
                                                                                            ccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCgg          >  2:5991288/1‑101 (MQ=255)
                                                                                              gcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAAGCACCTTCAAAAGTCGCTCCCCCCGCgggg        >  2:2169227/1‑101 (MQ=255)
                                                                                                tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGc      <  1:9200520/101‑1 (MQ=255)
                                                                                                tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGc      <  1:7709404/101‑1 (MQ=255)
                                                                                                tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGc      >  2:1592169/1‑101 (MQ=255)
                                                                                                tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCCCGGGGGc      >  1:7366324/1‑101 (MQ=255)
                                                                                                 gTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCg     >  1:864594/1‑101 (MQ=255)
                                                                                                 gTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCg     >  1:894380/1‑101 (MQ=255)
                                                                                                   ctcACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTg   >  2:7099442/1‑101 (MQ=255)
                                                                                                   ctcACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTg   >  2:480721/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
AAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397794‑1397994

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG