Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,398,422 C→G 100% intergenic (+726/+1172) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,398,4220C→G100.0% 1494.2 401intergenic (+726/+1172)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (193/208):  ref base (0/0):  total (193/208)

Only 100 of 413 total aligned reads displayed.

GTTGGGGAGCGAGAACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGCCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAACGCGAG  >  NC_014034/1398325‑1398519
                                                                                                 |                                                                                                 
 ttGGGGAGCGAGAACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGt                                                                                               <  2:6532558/101‑1 (MQ=255)
  tGGGGAGCGAGAACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTg                                                                                              >  1:1696297/1‑101 (MQ=255)
     ggAGCGAGAACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTg                                                                                           >  2:9052572/1‑101 (MQ=255)
      gAGCGAGAACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGt                                                                                          >  2:8562400/1‑101 (MQ=255)
      gAGCGAGAACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGt                                                                                          <  2:1581501/101‑1 (MQ=255)
       aGCGAGAACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGtt                                                                                         <  2:2379058/101‑1 (MQ=255)
         cGAGAACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGGGCTGTTcc                                                                                       >  1:6757743/1‑101 (MQ=255)
          gagaACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCt                                                                                      <  1:4187136/101‑1 (MQ=255)
          gagaACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCt                                                                                      >  1:7182241/1‑101 (MQ=255)
          gagaACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCt                                                                                      <  1:2071177/101‑1 (MQ=255)
          gagaACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCt                                                                                      >  2:2038335/1‑101 (MQ=255)
          gagaACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCt                                                                                      <  2:4175156/101‑1 (MQ=255)
            gaACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGGGCTGTTCCTgg                                                                                    >  2:1312387/1‑100 (MQ=255)
             aaCCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGcg                                                                                   >  1:98684/1‑101 (MQ=255)
              aCCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTGATACGGGACGATGGCGGGGCTGTTCCTGcgg                                                                                  >  1:5866116/1‑101 (MQ=255)
              aCCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGcgg                                                                                  >  1:8740154/1‑101 (MQ=255)
               ccGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGcgga                                                                                 <  2:4909835/101‑1 (MQ=255)
               ccGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGcgga                                                                                 <  1:1031052/101‑1 (MQ=255)
                 ggCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGcggacc                                                                               <  2:7766625/101‑1 (MQ=255)
                  gCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGcggaccg                                                                              >  1:1276765/1‑101 (MQ=255)
                   cTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGcggaccgg                                                                             <  1:2373444/101‑1 (MQ=255)
                     aCGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGcggaccggac                                                                           <  1:1319290/101‑1 (MQ=255)
                     aCGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGcggaccggac                                                                           >  2:2892102/1‑101 (MQ=255)
                         tcggtTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACgggg                                                                       >  2:7145947/1‑101 (MQ=255)
                          cggtTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGgt                                                                      <  1:6297059/101‑1 (MQ=255)
                          cggtTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGgt                                                                      <  2:4503471/101‑1 (MQ=255)
                           ggtTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACggggcg                                                                     >  2:1423232/1‑99 (MQ=255)
                           ggtTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGgtg                                                                     >  1:2548794/1‑101 (MQ=255)
                            gtTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACggggggg                                                                    >  2:435794/1‑98 (MQ=255)
                            gtTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGgggt                                                                    >  1:685068/1‑101 (MQ=255)
                            gtTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGgggt                                                                    >  1:837960/1‑101 (MQ=255)
                             ttCGAGACCAGTGTCGCCGACTGTCGCCACGCGGATGCCGCCCCCCGCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTc                                                                   <  1:5993544/101‑1 (MQ=255)
                              tCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCg                                                                  <  1:8258672/101‑1 (MQ=255)
                               cGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCgg                                                                 >  2:5443353/1‑101 (MQ=255)
                               cGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCgg                                                                 >  1:3465280/1‑101 (MQ=255)
                                 agaCCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGgcc                                                               <  2:6757472/101‑1 (MQ=255)
                                    ccAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGgcctgc                                                            <  1:2111160/101‑1 (MQ=255)
                                     cAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGgcctgcc                                                           >  1:1407580/1‑101 (MQ=255)
                                     cAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGgcctgcc                                                           >  1:9011727/1‑101 (MQ=255)
                                      aGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGGGTCGgcctgcct                                                          >  2:9147194/1‑101 (MQ=255)
                                         gtCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCg                                                       >  1:741684/1‑101 (MQ=255)
                                           cgcgGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCggg                                                     >  2:87280/1‑101 (MQ=255)
                                            gcgGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGt                                                    >  2:8390304/1‑101 (MQ=255)
                                             cgGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTc                                                   >  1:2161261/1‑101 (MQ=255)
                                             cgGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTc                                                   <  1:3332926/101‑1 (MQ=255)
                                             cgGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTc                                                   >  2:3180205/1‑101 (MQ=255)
                                              ggACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCg                                                  >  2:537868/1‑101 (MQ=255)
                                               gACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGc                                                 <  1:359755/101‑1 (MQ=255)
                                               gACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGc                                                 <  1:3200726/101‑1 (MQ=255)
                                                aCTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCa                                                >  1:5169291/1‑101 (MQ=255)
                                                  tGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCaaa                                              <  1:8778478/101‑1 (MQ=255)
                                                  tGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCaaa                                              <  1:7772474/101‑1 (MQ=255)
                                                  tGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCaaa                                              >  1:1788146/1‑101 (MQ=255)
                                                   gTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCCTACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGGGTGGGCCGGCCGGGggggggccaaa                                             >  1:2940362/1‑93 (MQ=255)
                                                   gTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCaaaa                                             <  2:3381513/101‑1 (MQ=255)
                                                    tCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAAc                                            <  1:8024907/101‑1 (MQ=255)
                                                      gCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGCCCGGACGGGGGGTCGGCCTGCCTGCGGGTCGCAAAACgg                                          >  1:387865/1‑101 (MQ=255)
                                                       ccACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGa                                         <  2:2626469/101‑1 (MQ=255)
                                                           gcgGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATgg                                     <  2:1713264/101‑1 (MQ=255)
                                                            cgGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGa                                    >  2:8208886/1‑101 (MQ=255)
                                                            cgGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGa                                    >  2:1231683/1‑101 (MQ=255)
                                                             ggATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGaa                                   <  1:9064909/101‑1 (MQ=255)
                                                             ggATGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCGGTTCCTGCGGACCGGACGGGGTGGCGGCCTGCCTGCGGGTCGCAAAACGGAATGGaa                                   <  1:1838984/101‑1 (MQ=255)
                                                                tGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATg                                <  2:3037347/101‑1 (MQ=255)
                                                                tGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATg                                <  2:7288414/101‑1 (MQ=255)
                                                                tGCCGCCCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACCGAATGGAAATg                                <  2:7869735/101‑1 (MQ=255)
                                                                    gccCCCCTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAgcg                            <  1:4062033/101‑1 (MQ=255)
                                                                      cccccTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGcgcg                          <  1:7146251/101‑1 (MQ=255)
                                                                        cccTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGC.GGTCGCAAAACGGAATGGAAATGAGCGCGAcc                       <  2:7635169/101‑1 (MQ=255)
                                                                         ccTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGAcc                       >  1:3091394/1‑101 (MQ=255)
                                                                         ccTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGAcc                       <  2:5016813/101‑1 (MQ=255)
                                                                          cTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCg                      <  2:8094743/101‑1 (MQ=255)
                                                                          cTCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCg                      <  2:6399848/101‑1 (MQ=255)
                                                                           tCGGCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCgg                     >  1:6410390/1‑101 (MQ=255)
                                                                             ggCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCg                   >  1:4749204/1‑101 (MQ=255)
                                                                             ggCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCg                   >  1:4602588/1‑101 (MQ=255)
                                                                              gCCCTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGt                  >  1:567575/1‑101 (MQ=255)
                                                                               cgcTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTg                 <  2:8843473/99‑1 (MQ=255)
                                                                                ccTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTgc                <  1:2892200/101‑1 (MQ=255)
                                                                                ccTCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTgc                >  2:5836819/1‑101 (MQ=255)
                                                                                  tCATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTgcgc              >  1:7977078/1‑101 (MQ=255)
                                                                                   cATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGcc             >  1:7031916/1‑101 (MQ=255)
                                                                                   cATACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGcc             >  2:3991419/1‑101 (MQ=255)
                                                                                     tACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTc           >  1:2783583/1‑101 (MQ=255)
                                                                                     tACGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTc           <  1:8556376/101‑1 (MQ=255)
                                                                                     tACGGGACGACGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACTGGCGTGCGCCTc           <  1:6531128/101‑1 (MQ=255)
                                                                                      aCGGGACGATGGCGGTGCTGTTCCTGCGGACCGGCCGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCCCg          >  1:5391854/1‑101 (MQ=255)
                                                                                      aCGGGACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCg          >  2:1650281/1‑101 (MQ=255)
                                                                                        gggACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGaa        <  1:4457144/101‑1 (MQ=255)
                                                                                        gggACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGaa        >  2:4655888/1‑101 (MQ=255)
                                                                                         ggACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAAc       <  1:4987351/101‑1 (MQ=255)
                                                                                         ggACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAAc       >  2:2520903/1‑101 (MQ=255)
                                                                                         ggACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAAAGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAAc       <  1:6077699/101‑1 (MQ=255)
                                                                                          gACGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGGGCGCCTCGAAcg      >  1:6955918/1‑101 (MQ=255)
                                                                                           gcGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAAcgc     <  2:3590104/100‑1 (MQ=255)
                                                                                           aCGATGGCGGTGCTGTTCCTGCGGACCGGCCGGGGTGTCGGCCTGTCTGCGTGTCGCAAAACGGGATGGACATGAGCTCGACCGGCGTGTGCCtttgaggc     >  2:7426878/1‑94 (MQ=255)
                                                                                            cGATGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAAcgcg    >  2:6086428/1‑101 (MQ=255)
                                                                                              aTGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAACGCGAg  <  1:1430131/101‑1 (MQ=255)
                                                                                              aTGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAACGCGAg  >  2:7071094/1‑101 (MQ=255)
                                                                                              aTGGCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAACGCGAg  >  2:5633864/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
GTTGGGGAGCGAGAACCGGCTACGGTCGGTTCGAGACCAGTGTCGCGGACTGTCGCCACGCGGATGCCGCCCCCCTCGGCCCTCATACGGGACGATGCCGGTGCTGTTCCTGCGGACCGGACGGGGTGTCGGCCTGCCTGCGGGTCGCAAAACGGAATGGAAATGAGCGCGACCGGCGTGCGCCTCGAACGCGAG  >  NC_014034/1398325‑1398519

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG