Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,386 C→A 20.4% intergenic (‑165/‑793) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3860C→A20.4% 300.2 0.0 0.0534intergenic (‑165/‑793)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (51/58):  ref base (198/227):  total (249/285)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 579 total aligned reads displayed.

GTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCT  >  NC_014034/1434286‑1434486
                                                                                                       |                                                                                                      
gTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..Gtctc                                                                                                        <  1:6979202/101‑1 (MQ=255)
 tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                       <  1:1755017/101‑1 (MQ=255)
  atatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCg                                                                                                      >  1:5782060/1‑101 (MQ=255)
  atatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GGCTCCg                                                                                                      >  2:1425957/1‑101 (MQ=255)
    atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGct                                                                                                    <  1:4695790/101‑1 (MQ=255)
     tGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctc                                                                                                   >  1:1143794/1‑101 (MQ=255)
     tGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctc                                                                                                   >  1:1576154/1‑101 (MQ=255)
     tGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctc                                                                                                   >  1:682962/1‑101 (MQ=255)
      gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                                  <  1:848547/101‑1 (MQ=255)
          cGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCtt                                                                                              <  1:9049247/101‑1 (MQ=255)
          cGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCtt                                                                                              <  2:6712086/101‑1 (MQ=255)
           gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                             <  2:712647/101‑1 (MQ=255)
           gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTCCTTc                                                                                             >  2:5823208/1‑101 (MQ=255)
             ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCAc                                                                                           <  1:4828454/101‑1 (MQ=255)
              tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                          <  1:8741047/101‑1 (MQ=255)
               aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                        <  2:5000470/101‑1 (MQ=255)
                 cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCt                                                                                      >  1:6301398/1‑101 (MQ=255)
                    ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCTCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.g                                                                                  >  1:3371110/1‑101 (MQ=255)
                    ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.g                                                                                  <  2:3756836/101‑1 (MQ=255)
                      aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTc                                                                                <  2:3687396/101‑1 (MQ=255)
                       tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCa                                                                               <  1:4037232/101‑1 (MQ=255)
                        cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCaa                                                                              >  2:1041980/1‑101 (MQ=255)
                         gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAAc                                                                             >  2:8528440/1‑101 (MQ=255)
                         gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAAc                                                                             <  2:7940092/101‑1 (MQ=255)
                          aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGAT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAAcc                                                                            >  2:6086804/1‑101 (MQ=255)
                            aCAGGCTGCTTTGGGCGGAACCCCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCtt                                                                          <  2:1628571/101‑1 (MQ=255)
                            aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCtt                                                                          <  2:9149676/101‑1 (MQ=255)
                            aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGGCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCCCG.GCTGC.GTCAACCtt                                                                          >  1:6568961/1‑101 (MQ=255)
                                gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCg                                                                      <  1:5649584/101‑1 (MQ=255)
                                  tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCt                                                                    <  2:2307424/101‑1 (MQ=255)
                                  tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCt                                                                    <  2:4574614/101‑1 (MQ=255)
                                  tgcgtggggCGGAACACCTGATCCTGCTGGGTCCCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCt                                                                    <  2:7515828/95‑1 (MQ=255)
                                       tGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTccccc                                                               <  2:6974644/101‑1 (MQ=255)
                                              aaCACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCgggg                                                        <  1:5103982/101‑1 (MQ=255)
                                                cacCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                      <  2:7241718/101‑1 (MQ=255)
                                                   cTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                   >  2:6222430/1‑101 (MQ=255)
                                                   cTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                   <  2:5379915/101‑1 (MQ=255)
                                                    tGATCCTGCTGGGTCGCTCCCCCCCCCGGGGGGGGGGATCGAACC..GCCTCCGCTCTGCTT.ACC.GCCGCGGTCACCCTTGTCGCTCCCCCCCCGGGGGCggg                                                   >  2:1141811/1‑99 (MQ=255)
                                                    tGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                                  >  1:7167006/1‑101 (MQ=255)
                                                      gcgggctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTg                                                   >  1:4549741/11‑101 (MQ=2)
                                                      aTCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGac                                                >  2:225014/1‑100 (MQ=255)
                                                      aTCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:302348/101‑1 (MQ=255)
                                                      aTCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:3032801/101‑1 (MQ=255)
                                                       tCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:2474972/101‑1 (MQ=255)
                                                       tCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:2666743/101‑1 (MQ=255)
                                                        ccTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  1:4491071/101‑1 (MQ=255)
                                                        ccTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  2:4788773/101‑1 (MQ=255)
                                                        ccTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  1:3796571/101‑1 (MQ=255)
                                                        ccTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  2:2689756/101‑1 (MQ=255)
                                                         ggctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                >  2:4926267/8‑101 (MQ=2)
                                                         ggctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:6010675/94‑1 (MQ=2)
                                                         ctgctgGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  1:4433366/1‑101 (MQ=255)
                                                          gctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                               >  1:2038027/7‑101 (MQ=2)
                                                          gctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:3269516/95‑1 (MQ=2)
                                                           ctatcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  2:1800390/96‑1 (MQ=2)
                                                            gatcttCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                          <  1:8201281/96‑1 (MQ=255)
                                                            ctgGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                          >  2:7609350/1‑101 (MQ=255)
                                                             tgGGTCGCT.CCCCCCGCGGGGGCGTGGATCGACAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                         <  2:6752980/101‑1 (MQ=255)
                                                              tcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  1:3545041/99‑1 (MQ=2)
                                                              gggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                        <  1:5887868/101‑1 (MQ=255)
                                                               ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                       <  2:5517771/101‑1 (MQ=255)
                                                               ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                       <  2:7847782/101‑1 (MQ=255)
                                                               cgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                          >  1:7677790/2‑101 (MQ=2)
                                                               cgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAac                                          >  2:6381122/2‑101 (MQ=2)
                                                                gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                         >  1:5303036/1‑100 (MQ=2)
                                                                gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                      <  1:6222669/101‑1 (MQ=255)
                                                                gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                      <  1:7824828/101‑1 (MQ=255)
                                                                 tCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacagatc                                     >  2:8200932/1‑97 (MQ=255)
                                                                  cGCT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                       <  1:7530842/101‑4 (MQ=2)
                                                                   gCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                   <  2:1530220/101‑1 (MQ=255)
                                                                    cT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                     >  2:4191838/1‑96 (MQ=2)
                                                                    cT.CCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                     >  2:188997/1‑96 (MQ=2)
                                                                     t.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                 >  2:3326647/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                   >  1:8569130/1‑94 (MQ=2)
                                                                       ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                >  1:9128754/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                >  2:2358207/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                >  1:7881878/1‑101 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                               <  2:2895605/101‑1 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGG.ATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                <  2:837130/101‑11 (MQ=0)
                                                                          cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                             <  2:5292689/101‑1 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAAC..GGCTCCGCTCTGCTTCACG.GCTGC.GGCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                            <  2:9000106/101‑1 (MQ=255)
                                                                                 gggGGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                      >  1:8805415/1‑101 (MQ=255)
                                                                                 gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                      >  1:7367438/1‑101 (MQ=255)
                                                                                 gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                      <  2:5675891/101‑1 (MQ=255)
                                                                                  ggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                     <  1:5414358/101‑1 (MQ=255)
                                                                                   gCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                    <  2:3954443/101‑1 (MQ=255)
                                                                                      tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                 >  1:414131/1‑101 (MQ=255)
                                                                                       ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                <  1:604482/101‑1 (MQ=255)
                                                                                       ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCCCGGCGGCGTGGATCGAAACACTGAGTGCATGGCGCGGGGGGGCAg                >  1:1404722/1‑101 (MQ=25)
                                                                                         aTCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc              >  1:2245958/1‑101 (MQ=255)
                                                                                           cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt            >  1:1968616/1‑101 (MQ=255)
                                                                                           cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt            >  1:107803/1‑101 (MQ=255)
                                                                                            gAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt           <  2:2103229/101‑1 (MQ=255)
                                                                                             aaaC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg          <  1:79879/101‑1 (MQ=255)
                                                                                             aaaC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg          >  1:6346989/1‑101 (MQ=255)
                                                                                             aaaC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg          >  2:3225394/1‑101 (MQ=255)
                                                                                              aaC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc         <  2:8805010/101‑1 (MQ=255)
                                                                                                   gTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt      <  2:352748/101‑1 (MQ=255)
                                                                                                   gTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt      <  2:4445949/101‑1 (MQ=255)
                                                                                                    tctcCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc     >  2:6479084/1‑101 (MQ=255)
                                                                                                       |                                                                                                      
GTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGC.GTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCT  >  NC_014034/1434286‑1434486

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG